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  1. AU="Dongho Lee"
  2. AU=Houser Steven R.
  3. AU="Morgom M.M."
  4. AU="Jordana-Comajuncosa, Rosa"
  5. AU="Kaushansky, Alexis"
  6. AU="Bhatjiwale, Mohinish"
  7. AU="Velu, Chinavenmeni S"
  8. AU=Trayanova Natalia A
  9. AU=Jimeno-Gonzlez Silvia
  10. AU=Bussolino F
  11. AU="Almulla, Hanan"
  12. AU="Chen, Wenmei"
  13. AU=Zeng Weiqing

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  1. Artikel ; Online: Regulation of pseurotin A biosynthesis by GliZ and zinc in Aspergillus fumigatus

    Hyewon Seo / Suzie Kang / Dongho Lee / Cheol-Won Yun

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Band 12

    Abstract: Abstract Recently, we reported that zinc regulates gliotoxin biosynthesis via ZafA, which is a zinc-responsive transcriptional activator. From an HPLC analysis of culture media of Aspergillus fumigatus, we found a trend of decreasing gliotoxin production ...

    Abstract Abstract Recently, we reported that zinc regulates gliotoxin biosynthesis via ZafA, which is a zinc-responsive transcriptional activator. From an HPLC analysis of culture media of Aspergillus fumigatus, we found a trend of decreasing gliotoxin production but increasing pseurotin A and fumagillin production in proportion to the zinc concentration. The expression of the genes involved in pseurotin A biosynthesis was upregulated under high zinc concentrations. Furthermore, upregulated expression of pseurotin A biosynthetic genes and higher production of pseurotin A were observed in the zafA deletion strain. Interestingly, the deletion of gliZ, a transcriptional activator of gliotoxin biosynthesis genes, resulted in upregulated expression of pseurotin A biosynthetic genes and increased production of pseurotin A. We detected upregulation of fumR expression in the gliZ and zafA deletion mutants. The overexpression of gliZ observed in the zafA deletion mutant resulted in the failure of the mutant to increase pseurotin A production, which is a phenotype of the zafA deletion mutant. These results suggest that ZafA sequentially regulates pseurotin A biosynthesis through GliZ. Finally, we found through a murine virulence test that the gliZ and fumR double-deletion mutants showed a delayed death rate compared with the single-deletion mutants of either gliZ or fumR. Taken together, these results suggested that the biosynthesis of gliotoxin and pseurotin A are regulated in opposite ways by zinc utilization and that each secondary metabolite is synthesized when the synthesis of another secondary metabolite fails to protect it against the defense system of the host.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2023-02-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Special-Length-Priority Algorithm to Minimize Reinforcing Bar-Cutting Waste for Sustainable Construction

    Dongho Lee / Seunghyun Son / Doyeong Kim / Sunkuk Kim

    Sustainability, Vol 12, Iss 5950, p

    2020  Band 5950

    Abstract: Reinforcing bars (rebar), which have the most embodied carbon dioxide (CO 2 ) per unit weight in built environments, generate a significant amount of cutting waste during the construction phase. Excessive cutting waste not only increases the construction ...

    Abstract Reinforcing bars (rebar), which have the most embodied carbon dioxide (CO 2 ) per unit weight in built environments, generate a significant amount of cutting waste during the construction phase. Excessive cutting waste not only increases the construction cost but also contributes to a significant amount of CO 2 emissions. The objective of this paper is to propose a special-length-priority cutting waste minimization (CWM) algorithm for rebar, for sustainable construction. In the proposed algorithms, the minimization method by special and stock lengths was applied. The minimization by special length was performed first, and then the combination by stock length was performed for the remaining rebar. As a result of verifying the proposed algorithms through a case application, it was confirmed that the quantity of rebar was reduced by 6.04% compared with the actual quantity used. In the case building, a CO 2 emissions reduction of 406.6 ton-CO 2 and a cost savings of USD 119,306 were confirmed. When the results of this paper are applied in practice, they will be used as a tool for sustainable construction management as well as for construction cost reduction.
    Schlagwörter rebar work ; cutting waste ; minimization ; sustainable construction ; CO 2 emission ; cutting stock problem ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
    Thema/Rubrik (Code) 690 ; 670
    Sprache Englisch
    Erscheinungsdatum 2020-07-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Antihyperuricemic Effect of Dendropanax morbifera Leaf Extract in Rodent Models

    Dongho Lee / Jin-Kyoung Kim / Yongjae Han / Kwang Il Park

    Evidence-Based Complementary and Alternative Medicine, Vol

    2021  Band 2021

    Abstract: Dendropanax morbifera is a well-known traditional medicine used in China and Korea to treat intestinal disorders, urosis, diuresis, and chronic glomerulonephritis. Hyperuricemia is a metabolic disorder characterized by a high uric acid level in serum due ...

    Abstract Dendropanax morbifera is a well-known traditional medicine used in China and Korea to treat intestinal disorders, urosis, diuresis, and chronic glomerulonephritis. Hyperuricemia is a metabolic disorder characterized by a high uric acid level in serum due to an imbalance between uric acid production and excretion and causes gout. Recently, the prevalence of hyperuricemia worldwide has been continuously increasing. Xanthine oxidase (XOD) inhibitors (allopurinol (ALP) and febuxostat) and uricosuric agents (benzbromarone and probenecid) are used to treat hyperuricemia clinically. However, because these drugs are poorly tolerated and cause side effects, such as kidney diseases, hepatotoxicity, gastrointestinal symptoms, and hypersensitivity syndrome, only a limited number of drugs are available. We investigated the antihyperuricemic effects of Dendropanax morbifera leaf ethanol extract (DMLE) and its underlying mechanisms of action through in vitro and in vivo studies. We evaluated uric acid levels in serum and urine, and xanthine oxidase (XOD) inhibition activity in the serum and liver tissue of a hyperuricemic rat model of potassium oxonate (PO)-induced hyperuricemic rats. In vitro study, XOD-inhibitory activity was the lowest among the test substances at the IC50 of ALP. However, the IC50 of DMLE-70 was significantly low compared with that of other DMLEs (p<0.05). In PO-induced hyperuricemic rats, uric acid (UA) levels in serum and urine were significantly reduced in all DMLE-70 and allopurinol-treated (ALT) groups than in the PC group (p<0.05). UA levels in urine were lower than those in serum in all DME groups. In PO-induced hyperuricemic rats, DMEE-200 reduced UA concentration in serum and increased UA excretion in the urine. These findings suggest that DMLE exerts antihyperuricemic and uricosuric effects on promoting UA excretion by enhanced secretion and inhibition of UA reabsorption in the kidneys. Thus, DMLE may be a potential treatment for hyperuricemia and gout.
    Schlagwörter Other systems of medicine ; RZ201-999
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2021-01-01T00:00:00Z
    Verlag Hindawi Limited
    Dokumenttyp Artikel ; Online
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  4. Artikel ; Online: Throughput screening of Bacillus subtilis strains that abundantly secrete surfactin in vitro identifies effective probiotic candidates

    Dongho Lee / Taehoon Oh / Bongseok Kang / Jong Seok Ahn / Yangrae Cho

    PLoS ONE, Vol 17, Iss

    2022  Band 11

    Abstract: Since the prohibition of antibiotics as animal growth promoters, demand for effective probiotic strains has steadily increased. The goal is to maintain productivity and mitigate environmental concerns in the livestock industry. There are many probiotic ... ...

    Abstract Since the prohibition of antibiotics as animal growth promoters, demand for effective probiotic strains has steadily increased. The goal is to maintain productivity and mitigate environmental concerns in the livestock industry. There are many probiotic animal-diet supplements available, over 2,000 products in the Republic of Korea alone, with little explanation about the desirable properties of each probiotic strain. The purpose of this study was to describe the underlying logic and methods used to select two novel strains of probiotic candidates. To economically screen these candidates, the abundance of surfactin secreted was used as an in vitro marker. We used a modified oil-misting method to screen ~2,000 spore-forming bacteria for novel strains of Bacillus subtilis. Of these, 18 strains were initially selected based on the semiquantitative criterion that they secreted more surfactin than B. subtilis ATCC21322 on Luria-Berani (LB) agar plates. The whole genome sequence was determined for two of the 18 strains to verify their identity. A phylogeny of 1,162 orthologous genes, genome contents, and genome organization confirmed them as novel strains. The surfactin profiles produced by these two strains consisted of at least four isoforms similar to standard surfactin and enhanced cellulase activities up to 50%. Four fractionated individual isoforms of surfactin suppressed inflammation induced by lipopolysaccharides. The half-maximal inhibitory concentration (IC50) was about 20 μM for each isoform. Both selected strains were susceptible to seven important antibiotics. Our results implied that an abundant secretion of surfactin was a useful biomarker in vitro and could be utilized for mining probiotic candidates through high-throughput screening of environmental samples.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2022-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
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  5. Artikel ; Online: Citromycin Isolated from the Antarctic Marine-Derived Fungi, Sporothrix sp., Inhibits Ovarian Cancer Cell Invasion via Suppression of ERK Signaling

    He Yun Choi / Ji-Hye Ahn / Haeun Kwon / Joung Han Yim / Dongho Lee / Jung-Hye Choi

    Marine Drugs, Vol 20, Iss 275, p

    2022  Band 275

    Abstract: Recently, microorganisms and their metabolites in the Antarctic marine environment have attracted attention as useful sources for novel therapeutics, including anticancer drugs. Here, we investigated the effects of citromycin, isolated from the Antarctic ...

    Abstract Recently, microorganisms and their metabolites in the Antarctic marine environment have attracted attention as useful sources for novel therapeutics, including anticancer drugs. Here, we investigated the effects of citromycin, isolated from the Antarctic marine-derived fungus, Sporothrix sp., on human ovarian cancer cells. Citromycin inhibited the migration and invasion of human ovarian cancer SKOV3 and A2780 cells, but had no cytotoxic activity against them. Additionally, it inhibited the expression of epithelial–mesenchymal transition (EMT) markers and the activation of matrix metalloproteinase (MMP)-2 and MMP9. Moreover, extracellular signal-regulated kinase (ERK)-1/2 signaling was inhibited after citromycin treatment, and the ectopic expression of ERK negated the anti-invasive activity of citromycin. Our findings suggest that citromycin inhibits the migration and invasion of human ovarian cancer cells by downregulating the expression levels of EMT markers and MMP-2/9 via inhibition of the ERK1/2 pathway.
    Schlagwörter citromycin ; Sporothrix sp ; ovarian cancer ; invasion ; MMP2 and MMP9 ; ERK1/2 pathway ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2022-04-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Identification of the Antidepressant Function of the Edible Mushroom Pleurotus eryngii

    Yong-Sung Park / Subin Jang / Hyunkoo Lee / Suzie Kang / Hyewon Seo / Seoyeong Yeon / Dongho Lee / Cheol-Won Yun

    Journal of Fungi, Vol 7, Iss 190, p

    2021  Band 190

    Abstract: Pleurotus eryngii produces various functional molecules that mediate physiological functions in humans. Recently, we observed that P. eryngii produces molecules that have antidepressant functions. An ethanol extract of the fruiting body of P. eryngii was ...

    Abstract Pleurotus eryngii produces various functional molecules that mediate physiological functions in humans. Recently, we observed that P. eryngii produces molecules that have antidepressant functions. An ethanol extract of the fruiting body of P. eryngii was obtained, and the extract was purified by XAD-16 resin using an open column system. The ethanol eluate was separated by HPLC, and the fraction with an antidepressant function was identified. Using LC-MS, the molecular structure of the HPLC fraction with antidepressant function was identified as that of tryptamine, a functional molecule that is a tryptophan derivative. The antidepressant effect was identified from the ethanol extract, XAD-16 column eluate, and HPLC fraction by a serotonin receptor binding assay and a cell-based binding assay. Furthermore, a forced swimming test (FST) showed that the mice treated with purified fractions of P. eryngii exhibited decreased immobility time compared with nontreated mice. From these results, we suggest that the extract of P. eryngii has an antidepressant function and that it may be employed as an antidepressant health supplement.
    Schlagwörter antidepressants ; P. eryngii ; tryptamine ; mushrooms ; fungi ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2021-03-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Detection of Aberrant Glycosylation of Serum Haptoglobin for Gastric Cancer Diagnosis Using a Middle-Up-Down Glycoproteome Platform

    Seunghyup Jeong / Unyong Kim / Myungjin Oh / Jihyeon Nam / Sehoon Park / Yoonjin Choi / Dongho Lee / Jaehan Kim / Hyunjoo An

    Journal of Personalized Medicine, Vol 11, Iss 575, p

    2021  Band 575

    Abstract: Gastric cancer is a frequently occurring cancer and is the leading cause of cancer-related deaths. Recent studies have shown that aberrant glycosylation of serum haptoglobin is closely related to gastric cancer and has enormous potential for use in ... ...

    Abstract Gastric cancer is a frequently occurring cancer and is the leading cause of cancer-related deaths. Recent studies have shown that aberrant glycosylation of serum haptoglobin is closely related to gastric cancer and has enormous potential for use in diagnosis. However, there is no platform with high reliability and high reproducibility to comprehensively analyze haptoglobin glycosylation covering microheterogeneity to macroheterogeneity for clinical applications. In this study, we developed a middle-up-down glycoproteome platform for fast and accurate monitoring of haptoglobin glycosylation. This platform utilizes an online purification of LC for sample desalting, and an in silico haptoglobin glycopeptide library constructed by combining peptides and N-glycans to readily identify glycopeptides. In addition, site-specific glycosylation with glycan heterogeneity can be obtained through only a single MS analysis. Haptoglobin glycosylation in clinical samples consisting of healthy controls ( n = 47) and gastric cancer patients ( n = 43) was extensively investigated using three groups of tryptic glycopeptides: GP1 (including Asn184), GP2 (including Asn207 and Asn211), and GP3 (including Asn241). A total of 23 individual glycopeptides were determined as potential biomarkers ( p < 0.00001). In addition, to improve diagnostic efficacy, we derived representative group biomarkers with high AUC values (0.929 to 0.977) through logistic regression analysis for each GP group. It has been found that glycosylation of haptoglobin is highly associated with gastric cancer, especially the glycosite Asn241. Our assay not only allows to quickly and easily obtain information on glycosylation heterogeneity of a target glycoprotein but also makes it an efficient tool for biomarker discovery and clinical diagnosis.
    Schlagwörter gastric cancer ; middle-up-down ; haptoglobin ; glycopeptide ; biomarker ; mass spectrometry ; Medicine ; R
    Thema/Rubrik (Code) 610
    Sprache Englisch
    Erscheinungsdatum 2021-06-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel: Progress on ternary oxide-based photoanodes for use in photoelectrochemical cells for solar water splitting

    Lee, Dong Ki / Dongho Lee / Margaret A. Lumley / Kyoung-Shin Choi

    Chemical Society reviews. 2019 Apr. 1, v. 48, no. 7

    2019  

    Abstract: Solar water splitting using photoelectrochemical cells (PECs) has emerged as one of the most promising routes to produce hydrogen as a clean and renewable fuel source. Among various semiconductors that have been considered as photoelectrodes for use in ... ...

    Abstract Solar water splitting using photoelectrochemical cells (PECs) has emerged as one of the most promising routes to produce hydrogen as a clean and renewable fuel source. Among various semiconductors that have been considered as photoelectrodes for use in PECs, oxide-based photoanodes are particularly attractive because of their stability in aqueous media in addition to inexpensive and facile processing compared to other types of semiconductors. However, they typically suffer from poor charge carrier separation and transport. In the past few years, there has been tremendous progress in developing ternary oxide-based photoelectrodes, specifically, photoanodes. The use of ternary oxides provides more opportunities to tune the composition and electronic structure of the photoelectrode compared to binary oxides, thus providing more freedom to tune the photoelectrochemical properties. In this article, we outline the important characteristics to analyze when evaluating photoanodes and review the major recent progress made on the development of ternary oxide-based photoanodes. For each system, we highlight the favorable and unfavorable features and summarize the strategies utilized to address the challenges associated with each material. Finally, by combining our analyses of all the photoanodes surveyed in this review, we provide possible future research directions for each compound and an outlook for constructing more efficient oxide-based PECs. Overall, this review will provide a critical overview of current ternary oxide-based photoanodes and will serve as a platform for the design of future oxide-based PECs.
    Schlagwörter hydrogen ; oxides ; renewable energy sources ; semiconductors
    Sprache Englisch
    Erscheinungsverlauf 2019-0401
    Umfang p. 2126-2157.
    Erscheinungsort The Royal Society of Chemistry
    Dokumenttyp Artikel
    ZDB-ID 1472875-8
    ISSN 1460-4744 ; 0306-0012
    ISSN (online) 1460-4744
    ISSN 0306-0012
    DOI 10.1039/c8cs00761f
    Datenquelle NAL Katalog (AGRICOLA)

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  9. Artikel ; Online: Metabolomic Elucidation of the Effect of Sucrose on the Secondary Metabolite Profiles in Melissa officinalis by Ultraperformance Liquid Chromatography–Mass Spectrometry

    Sooah Kim / Jungyeon Kim / Nahyun Kim / Dongho Lee / Hojoung Lee / Dong-Yup Lee / Kyoung Heon Kim

    ACS Omega, Vol 5, Iss 51, Pp 33186-

    2020  Band 33195

    Schlagwörter Chemistry ; QD1-999
    Sprache Englisch
    Erscheinungsdatum 2020-12-01T00:00:00Z
    Verlag American Chemical Society
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  10. Artikel ; Online: Dianthiamides A–E, Proline-Containing Orbitides from Dianthus chinensis

    Jin Woo Lee / Jun Gu Kim / Jae Sang Han / Yong Beom Cho / Yu Jin Lee / Dongho Lee / Dae Hwan Shin / Jin Tae Hong / Mi Kyeong Lee / Bang Yeon Hwang

    Molecules, Vol 26, Iss 7275, p

    2021  Band 7275

    Abstract: Orbitides are plant-derived small cyclic peptides with a wide range of biological activities. Phytochemical investigation of the whole plants of Dianthus chinensis was performed with the aim to discover new bioactive orbitides. Five undescribed proline- ... ...

    Abstract Orbitides are plant-derived small cyclic peptides with a wide range of biological activities. Phytochemical investigation of the whole plants of Dianthus chinensis was performed with the aim to discover new bioactive orbitides. Five undescribed proline-containing orbitides, dianthiamides A–E ( 1 – 5 ), were isolated from a methanolic extract of Dianthus chinensis . Their structures were elucidated by extensive analysis of 1D and 2D NMR and HRESI–TOF–MS as well as ESI–MS/MS fragmentation data. The absolute configuration of the amino acid residues of compounds 1 – 5 was determined by Marfey’s method. All compounds were tested for their cytotoxic activity, and dianthiamide A ( 1 ) exhibited weak activity against A549 cell line with IC 50 value of 47.9 μM.
    Schlagwörter Dianthus chinensis ; Caryophyllaceae ; orbitide ; structure elucidation ; cytotoxic activity ; Organic chemistry ; QD241-441
    Sprache Englisch
    Erscheinungsdatum 2021-11-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
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