Article: Evaluation of a ddPCR Commercial Assay for the Absolute Quantification of the Monkeypox Virus West Africa in Clinical Samples.
Diagnostics (Basel, Switzerland)
2023 Volume 13, Issue 7
Abstract: Background: Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family : Methods: The ddPCR reaction was assessed as a duplex assay using : Results: Regarding the clinical samples, compared to routine ... ...
| Abstract | Background: Monkeypox virus (MPXV) is a double-stranded DNA virus belonging to the orthopoxvirus genus in the family Methods: The ddPCR reaction was assessed as a duplex assay using Results: Regarding the clinical samples, compared to routine molecular diagnostics, the ddPCR duplex assay showed 100% of specificity for all biological matrices and 100% sensitivity (10/10) for lesions, 100% (10/10) for rectal swabs, 90% (9/10) for pharyngeal swabs, and 60% (6/10) for whole blood. Conclusion: Overall, our data showed that the commercial ddPCR assay allowed the DNA detection of MPXV in 87.5% (35/40) of our cohort, highlighting useful technical indications for the different specimens with a potential greatest performance for skin lesions and rectal swabs. |
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| Language | English |
| Publishing date | 2023-04-04 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2662336-5 |
| ISSN | 2075-4418 |
| ISSN | 2075-4418 |
| DOI | 10.3390/diagnostics13071349 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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