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Article ; Online: A METHOD FOR RANKING DECISIONAL ALTERNATIVES USING INTUITIONISTIC FUZZY SETS

Dorin I. Lixandroiu

Journal of Smart Economic Growth, Vol 2, Iss

2017 Volume 2

Abstract: This paper proposes a new method for ranking the alternatives represented under the form of IFS. The method is analysed in comparison with other techniques used for ordering introduced by Xu (2007), Szmidt and Kacprzyk (2008). The order is determined ... ...

Abstract	This paper proposes a new method for ranking the alternatives represented under the form of IFS. The method is analysed in comparison with other techniques used for ordering introduced by Xu (2007), Szmidt and Kacprzyk (2008). The order is determined based on the distance to the ideal solution (the optimal point) and on the distance to the points and corresponding to the maximum level of non-membership, and of indeterminacy, respectively. The proposed method is applied in a multi-attribute decision model.
Keywords	Social Sciences ; H ; Economics as a science ; HB71-74
Language	English
Publishing date	2017-09-01T00:00:00Z
Publisher	Journal of Smart Economic Growth
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Androgenic ability and plant regeneration potential in some tomato varieties

Adriana BĂDULESCU / Anamaria M. DUMITRU / Andreea E. MANOLESCU / Dorin I. SUMEDREA / Carmen F. POPESCU

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Vol 50, Iss

2022 Volume 1

Abstract: Aiming to evaluate the in vitro regeneration potential, five varieties of tomatoes (Solanum lycopersicum) were studied for their response in anther culture. Anther explants at an early stage of microspore development were inoculated onto three culture ... ...

Abstract	Aiming to evaluate the in vitro regeneration potential, five varieties of tomatoes (Solanum lycopersicum) were studied for their response in anther culture. Anther explants at an early stage of microspore development were inoculated onto three culture media. The first differentiation processes were recorded during the first three weeks of culture, in darkness. The statistical analysis of the data recorded during the anther culture showed significant differences between genotypes regarding their specific response to culture conditions and the significant influence of the initiation medium composition in triggering the differentiation processes. Under the tested conditions were induced: the embryogenic potential in three genotypes (ʻȘtefănești 22ʼ, ʻCostate 21ʼ and ʻChihlimbarʼ) and the indirect organogenesis in two genotypes (ʻArgeș 20ʼ and ʻCostate 21ʼ). Morphological characteristics of anther-derived plants from ʻArgeș 20ʼ variety, grown in greenhouse conditions (growth rate, features of leaf, flower, and fruit), as well as analyses with nine SSR markers (banding patterns, the coefficient of genetic similarity, and the polymorphism information content) in DNA samples from each regenerant and the donor variety, provided clear evidence of the occurrence of spontaneous genetic variation during in vitro anther culture, and of the existence of somaclonal variation in regenerated plants. The amplified products obtained with SSR primers revealed a total number of scorable bands of 160 and a mean percentage of polymorphic bands of 21.09%. Two out of the nine tested primers, SSR63 and SLM6-7 proved to be efficient in detecting genetic differences not only among regenerants but also between them and the donor genotype.
Keywords	androgenic induction ; molecular markers ; phenotypic traits ; tissue culture ; variability ; Forestry ; SD1-669.5 ; Agriculture (General) ; S1-972
Subject code	580
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	AcademicPres
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Androgenic ability and plant regeneration potential in some tomato varieties

Adriana BĂDULESCU / Anamaria M. DUMITRU / Andreea E. MANOLESCU / Dorin I. SUMEDREA / Carmen F. POPESCU

Notulae botanicae Horti Agrobotanici Cluj-Napoca. 2022, v. 50, no. 1

2022

Abstract	Aiming to evaluate the in vitro regeneration potential, five varieties of tomatoes (Solanum lycopersicum) were studied for their response in anther culture. Anther explants at an early stage of microspore development were inoculated onto three culture media. The first differentiation processes were recorded during the first three weeks of culture, in darkness. The statistical analysis of the data recorded during the anther culture showed significant differences between genotypes regarding their specific response to culture conditions and the significant influence of the initiation medium composition in triggering the differentiation processes. Under the tested conditions were induced: the embryogenic potential in three genotypes (ʻȘtefănești 22ʼ, ʻCostate 21ʼ and ʻChihlimbarʼ) and the indirect organogenesis in two genotypes (ʻArgeș 20ʼ and ʻCostate 21ʼ). Morphological characteristics of anther-derived plants from ʻArgeș 20ʼ variety, grown in greenhouse conditions (growth rate, features of leaf, flower, and fruit), as well as analyses with nine SSR markers (banding patterns, the coefficient of genetic similarity, and the polymorphism information content) in DNA samples from each regenerant and the donor variety, provided clear evidence of the occurrence of spontaneous genetic variation during in vitro anther culture, and of the existence of somaclonal variation in regenerated plants. The amplified products obtained with SSR primers revealed a total number of scorable bands of 160 and a mean percentage of polymorphic bands of 21.09%. Two out of the nine tested primers, SSR63 and SLM6-7 proved to be efficient in detecting genetic differences not only among regenerants but also between them and the donor genotype.
Keywords	DNA ; Solanum lycopersicum ; anther culture ; flowers ; fruits ; genetic similarity ; genotype ; greenhouses ; leaves ; organogenesis ; somaclonal variation ; statistical analysis ; tomatoes
Language	English
Publishing place	AcademicPres
Document type	Article
ZDB-ID	2271404-2
ISSN	1842-4309 ; 0255-965X
ISSN (online)	1842-4309
ISSN	0255-965X
DOI	10.15835/nbha50112413
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Comparison of some DNA extraction methods from monovarietal must and wines

Anca P. ONACHE / Adriana BĂDULESCU / Anamaria M. DUMITRU / Dorin I. SUMEDREA / Carmen F. POPESCU

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Vol 49, Iss

2021 Volume 2

Abstract: The methods applied for DNA extraction from must and wine samples with monovarietal origin are presented and discussed aiming to prove the quality of extracted DNA and its good properties for further use in molecular tests. In the present research were ... ...

Abstract	The methods applied for DNA extraction from must and wine samples with monovarietal origin are presented and discussed aiming to prove the quality of extracted DNA and its good properties for further use in molecular tests. In the present research were compared four different DNA extraction methods from must and wine samples obtained from eleven V. vinifera varieties (five grapevine varieties for white wines and six grapevine varieties for red wines, respectively). Taking into consideration the amounts of obtained DNA, the concentrations and purities of the final DNA extracts, were stood out two modified methods. For all must samples, very efficient was the second method, which allowed obtaining a mean value of 87.9 ng µl-1 for the DNA concentration with 1.55 purity. Among the tested procedures, for monovarietal wine samples, the fourth method proved to be the most efficient which brought a mean value of 64.7 ng μl-1 for DNA concentration with 1.66 purity. This method adequate for wine samples involves two CTAB solution treatments and the RNase treatment applied before DNA resuspension. The DNA from must and wine extracts and the DNA from leaves of the corresponding grapevine varieties were amplified with five specific microsatellite primers (VVS2, VVMD27, VVMD32, VrZAG79 and VrZAG62). The aspects of pattern profiles were analysed in parallel and proved that the extracted DNA was suitable for amplification with these specific V. vinifera primers. The two selected extraction procedures are considered good for research purposes and ensure obtaining of good-quality extracted DNA from musts and one-year old wines.
Keywords	DNA quality ; grapevine ; spectrophotometry ; SSR markers ; traceability ; Forestry ; SD1-669.5 ; Agriculture (General) ; S1-972
Subject code	612
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	AcademicPres
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Comparison of some DNA extraction methods from monovarietal must and wines

ONACHE, Anca P. / BĂDULESCU, Adriana / DUMITRU, Anamaria M. / SUMEDREA, Dorin I. / POPESCU, Carmen F.

Notulae botanicae Horti Agrobotanici Cluj-Napoca. 2021, v. 49, no. 2

2021

Abstract	The methods applied for DNA extraction from must and wine samples with monovarietal origin are presented and discussed aiming to prove the quality of extracted DNA and its good properties for further use in molecular tests. In the present research were compared four different DNA extraction methods from must and wine samples obtained from eleven V. vinifera varieties (five grapevine varieties for white wines and six grapevine varieties for red wines, respectively). Taking into consideration the amounts of obtained DNA, the concentrations and purities of the final DNA extracts, were stood out two modified methods. For all must samples, very efficient was the second method, which allowed obtaining a mean value of 87.9 ng µl-1 for the DNA concentration with 1.55 purity. Among the tested procedures, for monovarietal wine samples, the fourth method proved to be the most efficient which brought a mean value of 64.7 ng μl-1 for DNA concentration with 1.66 purity. This method adequate for wine samples involves two CTAB solution treatments and the RNase treatment applied before DNA resuspension. The DNA from must and wine extracts and the DNA from leaves of the corresponding grapevine varieties were amplified with five specific microsatellite primers (VVS2, VVMD27, VVMD32, VrZAG79 and VrZAG62). The aspects of pattern profiles were analysed in parallel and proved that the extracted DNA was suitable for amplification with these specific V. vinifera primers. The two selected extraction procedures are considered good for research purposes and ensure obtaining of good-quality extracted DNA from musts and one-year old wines.
Keywords	DNA ; Vitis ; microsatellite repeats ; ribonucleases
Language	English
Publishing place	AcademicPres
Document type	Article
ZDB-ID	2271404-2
ISSN	1842-4309 ; 0255-965X
ISSN (online)	1842-4309
ISSN	0255-965X
DOI	10.15835/nbha49212349
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A molecular understanding of magnesium aluminium silicate - drug, drug - polymer, magnesium aluminium silicate - polymer nanocomposite complex interactions in modulating drug release: Towards zero order release.

Totea, A M / Dorin, I / Laity, P R / Sabin, Juan / Conway, B R / Waters, L / Asare-Addo, K

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

2020 Volume 154, Page(s) 270–282

Abstract: This study reports the use of ITC in understanding the thermodynamics occurring for a controlled release system in which complexation has been exploited. In this study, a model drug, propranolol hydrochloride (PPN) was complexed with magnesium aluminium ... ...

Abstract	This study reports the use of ITC in understanding the thermodynamics occurring for a controlled release system in which complexation has been exploited. In this study, a model drug, propranolol hydrochloride (PPN) was complexed with magnesium aluminium silicate (MAS) and these complexes were used in combination with polyethylene oxide (PEO) as a hydrophilic carrier at various concentrations to sustain the release of PPN. DSC, XRPD, ATR-FTIR and SEM/EDX were successfully used in characterising the produced complexes. 2D- SAXS data patterns for MAS and the produced complexes were shown to be symmetric and circular with the particles showing no preferred orientation at the nanometre scale. ITC studies showed differences between PPN adsorption onto MAS compared with PPN adsorption onto a MAS-PEO mixture. At both temperatures studied the binding affinity K
Language	English
Publishing date	2020-07-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1065368-5
ISSN	1873-3441 ; 0939-6411
ISSN (online)	1873-3441
ISSN	0939-6411
DOI	10.1016/j.ejpb.2020.07.027
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Article ; Online: New varieties of tomato - morphological aspects and molecular characterisation with RAPD and SSR markers

Adriana BĂDULESCU / Carmen F. POPESCU / Anamaria M. DUMITRU / Dorin I. SUMEDREA

Notulae Scientia Biologicae, Vol 12, Iss

2020 Volume 4

Abstract: This study presents the main morphological features and the first molecular investigations of four new tomato varieties (Solanum lycopersicum), aiming to obtain their complete characterisation. Evaluation with the standard descriptors for tomato revealed ...

Abstract	This study presents the main morphological features and the first molecular investigations of four new tomato varieties (Solanum lycopersicum), aiming to obtain their complete characterisation. Evaluation with the standard descriptors for tomato revealed specific and distinct traits for each analysed variety. The molecular analyses for variety identification started with testing three methods for DNA extraction. With an optimized method, which doesn’t need liquid nitrogen for plant tissue disruption, good quality DNA was obtained, in adequate quantities, and well preserved when stored at -20 °C. To highlight the genetic differences among the analysed tomato varieties, nine RAPD primers and ten SSR primers were tested. Of these, the optimal amplification protocols for five RAPD primers and five SSR primers were established. The amplified products obtained with RAPD primers revealed an average number of bands per primer of 8.8 and a total rate of polymorphism of 59.1%; with OPB10 primer was seen the highest number of DNA bands (11), and with OPA07 primer was registered the highest degree of genetic variability among the studied varieties (77.7%). Two SSR markers (SSR 20 and SSR T107) amplified monomorphic banding patterns corresponding to 170 base pairs and 250 base pairs, respectively, for all varieties; with SSR T7, SSR T62, and SSR T70 primers were generated multiple amplification bands, with a different distribution of the bands into the agarose gel for each analysed tomato variety.
Keywords	description ; genotypes ; molecular markers ; variability ; Agriculture (General) ; S1-972 ; Science (General) ; Q1-390
Subject code	630
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Society of Land Measurements and Cadastre from Transylvania (SMTCT)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Real time calorimetric characterisation of clay - drug complex dispersions and particles.

Totea, A M / Dorin, I / Gavrilov, G / Laity, P R / Conway, B R / Waters, L / Asare-Addo, K

International journal of pharmaceutics: X

2018 Volume 1, Page(s) 100003

Abstract: Isothermal titration calorimetry (ITC) along with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX) and high-performance liquid chromatography ...

Abstract	Isothermal titration calorimetry (ITC) along with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX) and high-performance liquid chromatography (HPLC) were employed to investigate the process of adsorption of propranolol hydrochloride (PPN) onto magnesium aluminium silicate (MAS) and to characterise the MAS-PPN particles formed upon complexation. The composition of MAS was confirmed by infrared (IR) spectroscopy and a calcimeter. The calorimetric results confirmed the binding between PPN and MAS at various pHs and temperatures. The overall change in enthalpy was found to be exothermic with a comparatively small entropic contribution to the total change in Gibbs free energy. These findings suggest that the binding process was enthalpically driven and entropically unfavourable (lower affinity) suggesting hydrogen bonding and electrostatic interactions dominating the interaction. The variation of pH and temperature did not have a great impact on the thermodynamics of the binding process, as observed from the similarity in enthalpy (ΔH), entropy (ΔS) or Gibbs free energy (ΔG). A slight reduction in the binding affinity (K
Language	English
Publishing date	2018-12-21
Publishing country	Netherlands
Document type	Journal Article
ISSN	2590-1567
ISSN (online)	2590-1567
DOI	10.1016/j.ijpx.2018.100003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Magnesium Aluminium Silicate-Metformin Hydrochloride Complexes - The Use of Isothermal Calorimetry for Probing Clay and Drug Nanocomplexations.

Totea, Ana-Maria / Dorin, Irina / Laity, Peter R / Conway, Barbara R / Waters, Laura / Asare-Addo, Kofi

Current drug delivery

2021 Volume 18, Issue 9, Page(s) 1280–1291

Abstract: Background: Studying complexation between a wide variety of drugs and clay is of high importance in expanding the knowledge about controlled drug delivery and its exploitation. This study reports the use of isothermal calorimetry (ITC) in understanding ... ...

Abstract	Background: Studying complexation between a wide variety of drugs and clay is of high importance in expanding the knowledge about controlled drug delivery and its exploitation. This study reports the use of isothermal calorimetry (ITC) in understanding the complexation process occurring between magnesium aluminium silicate (MAS) and metformin hydrochloride (MET), as a potentially controlled release drug delivery system. Objectives: To fully characterise and understand the complexes formed between MAS and MET and how that might impact on controlled release systems. Methods: MAS and MET complex dispersions and particles were formulated and analysed using ITC, DSC, XRPD, ATR-FTIR, SEM/EDX, digital microscopy and 2D-SAXS. Results: The calorimetric results confirmed the binding between MET and MAS at various pHs (5, 7 and 9) and temperatures (25 ºC and 37 ºC). The overall change in enthalpy was found to be exothermic with a comparatively small entropic contribution to the total change in Gibbs free energy, implying that the binding was an enthalpically driven process. These findings suggest that the binding process was dominated by hydrogen bonding and electrostatic interactions. pH and temperature variation did not have a great impact on the binding, as observed from the similarity in enthalpy (ΔH), entropy (ΔS) or Gibbs free energy (ΔG), with the reaction being only slightly more exothermic at pH 5 and at 37 ºC. 2D-SAXS was able to differentiate between MAS particulates and MAS-MET complexes when analysed in their liquid form suggesting the importance of appropriate methodology and instrumentation used in characterisation. Conclusion: ITC was successfully used in understanding the complexation process occurring between MAS and MET. Care and consideration however should thus be taken in the accurate determination and characterisation techniques for the formation of complexes for controlled release using MAS.
MeSH term(s)	Aluminum Compounds ; Calorimetry ; Clay ; Magnesium ; Magnesium Compounds ; Metformin ; Pharmaceutical Preparations ; Scattering, Small Angle ; Silicates ; Thermodynamics ; X-Ray Diffraction
Chemical Substances	Aluminum Compounds ; Magnesium Compounds ; Pharmaceutical Preparations ; Silicates ; aluminum magnesium silicate (6M3P64V0NC) ; Metformin (9100L32L2N) ; Magnesium (I38ZP9992A) ; Clay (T1FAD4SS2M)
Language	English
Publishing date	2021-04-19
Publishing country	United Arab Emirates
Document type	Journal Article
ZDB-ID	2185284-4
ISSN	1875-5704 ; 1567-2018
ISSN (online)	1875-5704
ISSN	1567-2018
DOI	10.2174/1567201818666210402125244
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Article ; Online: Thermodynamics of clay-drug complex dispersions: Isothermal titration calorimetry and high-performance liquid chromatography.

Totea, Ana-Maria / Sabin, Juan / Dorin, Irina / Hemming, Karl / Laity, Peter R / Conway, Barbara R / Waters, Laura / Asare-Addo, Kofi

Journal of pharmaceutical analysis

2019 Volume 10, Issue 1, Page(s) 78–85

Abstract: An understanding of the thermodynamics of the complexation process utilized in sustaining drug release in clay matrices is of great importance. Several characterisation techniques as well as isothermal calorimetry were utilized in investigating the ... ...

Abstract	An understanding of the thermodynamics of the complexation process utilized in sustaining drug release in clay matrices is of great importance. Several characterisation techniques as well as isothermal calorimetry were utilized in investigating the adsorption process of a model cationic drug (diltiazem hydrochloride, DIL) onto a pharmaceutical clay system (magnesium aluminium silicate, MAS). X-ray powder diffraction (XRPD), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and optical microscopy confirmed the successful formation of the DIL-MAS complexes. Drug quantification from the complexes demonstrated variable behaviour in the differing media used with DIL degrading to desacetyl diltiazem hydrochloride (DC-DIL) in the 2 M HCl media. Here also, the authors report for the first time two binding processes that occurred for DIL and MAS. A competitor binding model was thus proposed and the thermodynamics obtained suggested their binding processes to be enthalpy driven and entropically unfavourable. This information is of great importance for a formulator as care and consideration should be given with appropriate media selection as well as the nature of binding in complexes.
Language	English
Publishing date	2019-12-05
Publishing country	China
Document type	Journal Article
ZDB-ID	2630174-X
ISSN	2214-0883 ; 2095-1779
ISSN (online)	2214-0883
ISSN	2095-1779
DOI	10.1016/j.jpha.2019.12.001
Database	MEDical Literature Analysis and Retrieval System OnLINE

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