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Article ; Online: Epidemiological data and antifungal susceptibility in invasive fungal infections - a Romanian infectious diseases tertiary hospital’s experience. Preliminary report

Agrosoaie Radu / Streinu-Cercel Adrian / Azoicai Doina / Bejan Codrina / Dorobat Olga / Mihai Alexandru / Popoiu Mona / Rafila Alexandru

Romanian Journal of Laboratory Medicine, Vol 25, Iss 4, Pp 345-

2017 Volume 353

Abstract: Introduction: Invasive fungal infections have stood as an important research subject for the past 20 years, being considered as a crucial effect of advancing healthcare services. Low identification rates of invasive fungal infections in blood cultures ... ...

Abstract	Introduction: Invasive fungal infections have stood as an important research subject for the past 20 years, being considered as a crucial effect of advancing healthcare services. Low identification rates of invasive fungal infections in blood cultures and low sensibility of biomarkers determine empiric treatments which lead to a change in epidemiological data and antifungal susceptibility. The aim: The epidemiological evaluation of invasive fungal infections and the assessment of antifungal resistance related to this condition. Methods and material: An “antifungal stewardship” retrospective study was developed between January 2010 and April 2016. An epidemiological analysis was performed on 79 cases with proven invasive fungal infections in bloodstream, catheter, and cerebrospinal fluid. We considered: age, gender, HIV status, place of residence, and first option in medical practice of antifungal treatment. The laboratory analysis was performed by the Microbiology Laboratory at “Prof. Dr. Matei Bals” National Institute for Infectious Diseases, Bucharest. Minimum inhibitory concentrations (MIC’s) of 15 isolates were identified using colorimetric micro broth dilution panel YEASTONE ®YO10 and compared with susceptibilities obtained by VITEK2®C system. Candida parapsilosis ATCC 22019 was used as reference. Results: The incidence of invasive fungal infections was 3.7 on 1000 hospitalized patients. The age of the study population ranged between 12 and 83 years, and most were male (59%). The majority of subjects were from an urban area (84%), and 27% of them were HIV positive. The results obtained in VITEK2C® were similar with those from YEASTONE® YO10 for fluconazole, voriconazole, amphotericin B (100%), without any minor, major or very major errors. The fluconazole was the first option of treatment, followed by voriconazole, caspofungin, anidulafungin. In 37% of cases the first treatment option was replaced with a secondary antifungal therapy accordingly with antifungal breakpoints obtained by Vitek ®. Conclusions: ...
Keywords	invasive fungal infections ; antifungal stewardship ; vitek2® ; yeastone®yo10 ; Medicine ; R
Subject code	630
Language	English
Publishing date	2017-10-01T00:00:00Z
Publisher	Sciendo
Document type	Article ; Online
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Article ; Online: OXA-48-Carbapenemase-Producing Klebsiella pneumoniae infections - the first cases diagnosed in Romanian National Institute of Infectious Diseases

Popescu Cristina / Popescu Gabriel Adrian / Dorobăț Olga / Rafilă Alexandru / Tănase Diana / Mikula Claudia / Weissensteiner Gudrun / Huhulescu Steliana

Romanian Journal of Laboratory Medicine, Vol 25, Iss 1, Pp 55-

2017 Volume 61

Abstract: We report first description of clinical cases of OXA-48 carbapenemase-producing Klebsiella pneumoniae originating from patients hospitalized in the most important Infectious Diseases Hospital from Romania, between December 2012 and March 2013. All ... ...

Abstract	We report first description of clinical cases of OXA-48 carbapenemase-producing Klebsiella pneumoniae originating from patients hospitalized in the most important Infectious Diseases Hospital from Romania, between December 2012 and March 2013. All strains were isolated from patients who were previously admitted in surgical wards. None of the patients had been admitted in a hospital outside of Romania.
Keywords	klebsiella pneumoniae ; oxa-48 carbapenemase ; esbl ; risk factors for resistance ; Medicine ; R
Language	English
Publishing date	2017-03-01T00:00:00Z
Publisher	Sciendo
Document type	Article ; Online
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Article ; Online: Emergence of Carbapenemase-producing Enterobacteriaceae, a Public Health Threat

Rafila Alexandru / Talapan Daniela / Dorobăţ Olga Mihaela / Popescu Gabriel Adrian / Piţigoi Daniela / Florea Dragoş / Buicu Florin Corneliu

Romanian Journal of Laboratory Medicine, Vol 23, Iss 3, Pp 295-

a Romanian Infectious Disease Hospital Based Study / Emergenţa Enterobacteriaceaelor producătoare de carbapenemaze, o ameninţare pentru sănătatea publică: un studiu realizat într-un spital romanesc de boli infectioase

2015 Volume 301

Abstract: Introducere: Infecţiile produse de Enterobacteriaceae producătoare de carbapenemaze sunt tot mai frecvent întâlnite în practica medicală şi limitează serios antibioticoterapia. Am evaluat emergenţa enterobacteriilor rezistente la carbapeneme din ... ...

Abstract	Introducere: Infecţiile produse de Enterobacteriaceae producătoare de carbapenemaze sunt tot mai frecvent întâlnite în practica medicală şi limitează serios antibioticoterapia. Am evaluat emergenţa enterobacteriilor rezistente la carbapeneme din tulpinile izolate de la pacienţii internaţi în Institutul Naţional de Boli Infecţioase (INBI) din Bucureşti şi identificarea diferitelor tipuri de carbapenemaze, utilizând metode fenotipice.
Keywords	enterobacteriaceae ; resistance ; carbapenemase ; Medicine ; R
Language	English
Publishing date	2015-08-01T00:00:00Z
Publisher	Sciendo
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Article: Incidenţa şi pattern-ul de rezistenţă bacteriilor patogene izolate din tractul respirator inferior.

Dorobăţ, Olga Mihaela / Moisoiu, Adriana / Tălăpan, Daniela

Pneumologia (Bucharest, Romania)

2007 Volume 56, Issue 1, Page(s) 7–15

Abstract: The aim of present study was to evaluate the incidence and antibiotic susceptibility of pathogens in LRTI for patients in ICU/Surgery and pneumological wards from Marius Nasta Institute. A number of 938 strains isolated between September 1st 2004 and ... ...

Title translation	Incidence and resistance patterns of pathogens from lower respiratory tract infections (LRTI).
Abstract	The aim of present study was to evaluate the incidence and antibiotic susceptibility of pathogens in LRTI for patients in ICU/Surgery and pneumological wards from Marius Nasta Institute. A number of 938 strains isolated between September 1st 2004 and September 1st 2005 were identified by standard procedures and antimicrobial resistance was determined following CLSI approved standard. Imipenem-EDTA Double Disk Synergy test and Etest were used for detection of metallo-beta-lactamase producing isolates of P.aeruginosa. There were isolated 744 Gram-negative strains: H. influenzae 34.6%, P. aeruginosa 17.7%, H. parainfluenzae 15.9%, K. pneumoniae 8.6% and another spp. and 194 Gram-positive strains: 54.1% S.aureus and 45.9% S.pneumoniae. Among H. influenzae and H. parainfluenzae isolates, the highest resistance rate was to trimethoprim/sulfametoxazole (SXT 30.9% and 31.1%), followed by ampicillin (AMP 11.6% and 13.4%), chloramphenicol (C 4.5% and 5.1%) and clarithromycin (1.6% and 13.6%). P. aeruginosa strains showed a resistance rate between 7.9% to amikacin and 38.3% to cefoperazone. The resistance to imipenem (IPM) and meropenem (MEM) was close: 28.2% and 26.0%. From 36 P. aeruginosa IPM and multidrug resistant strains tested, 8 were probably producing metallo-beta-lactamase. For S. aureus the highest resistance rate was to penicillin 93.3% followed by erythromycin (E 45.7%), oxacillin 41.9% and CIP (33.3%); all strains were susceptible to vancomycin, teicoplanin and linezolid. From S. pneumoniae strains 13.4% were high resistant to penicillin and 39.3% were intermediate resistant. The resistance rate for other antibacterial agents was 64.0% to SXT, 18.8% to E, 8.3% to C and all strains were susceptible to levofloxacin. K. pneumoniae strains were resistant to cefepime (11.3%), CIP (7.8%) and there was no resistant strain to IPM and MEM.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Critical Care ; Drug Resistance, Multiple, Bacterial ; Gram-Negative Bacteria/drug effects ; Gram-Negative Bacteria/enzymology ; Gram-Negative Bacteria/isolation & purification ; Gram-Positive Bacteria/drug effects ; Gram-Positive Bacteria/enzymology ; Gram-Positive Bacteria/isolation & purification ; Humans ; Microbial Sensitivity Tests/methods ; Pulmonary Medicine ; Respiratory Tract Infections/microbiology ; Retrospective Studies ; Romania ; Surgery Department, Hospital ; beta-Lactamases/biosynthesis
Chemical Substances	Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
Language	Romanian
Publishing date	2007-01
Publishing country	Romania
Document type	Comparative Study ; English Abstract ; Journal Article ; Multicenter Study
ZDB-ID	2064988-5
ISSN	2247-059X ; 2067-2993 ; 1223-3056
ISSN (online)	2247-059X
ISSN	2067-2993 ; 1223-3056
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Article: Bacterii izolate din lichidul pleural si rezistenţa lor la antibiotice.

Dorobăţ, Olga-Mihaela / Moisoiu, Adriana / Tălăpan, Daniela

Pneumologia (Bucharest, Romania)

2006 Volume 55, Issue 2, Page(s) 47–51

Abstract: The aim of this study is to evaluate the frequency of microorganisms isolated from pleural fluids and their resistance to antimicrobial agents. A total of 272 pleural fluids were studied between July 2004 - July 2005 from the patients hospitalized in ICU/ ...

Title translation	Bacteria isolated from pleural fluid and their resistance to antimicrobials.
Abstract	The aim of this study is to evaluate the frequency of microorganisms isolated from pleural fluids and their resistance to antimicrobial agents. A total of 272 pleural fluids were studied between July 2004 - July 2005 from the patients hospitalized in ICU/surgery (127) and respiratory diseases wards (145) at Marius Nasta Institute. The laboratory investigations included: direct microscopy, cultures for aerobic and anaerobic bacteria, identification, disk diffusion method according with CLSI recommendations for resistance and Etest for detection of metallo-beta-lactamase producing isolates of Pseudomonas aeruginosa. Microorganisms were isolated from 159 samples (58.4%), 48 pleural fluids were positive only in microscopy (17.6%). The most frequent isolated strain was P. aeruginosa (49.6%), followed by Staphylococcus aureus (12.8%) and Enterobacteriaceae (11.2%) polymicrobial infections were mostly due to combinations of Pseudomonas with Enterobacteriaceae. For P. aeruginosa the resistance rate was higher than 71% for all beta-lactams. For aminoglycosides the lower resistance rate was to amikacin (18.0%). For quinolones, resistance of P. aeruginosa was 67.8% to ciprofloxacin. P. aeruginosa isolated from patients hospitalized in ICU/surgery were more resistant to some antimicrobials than the strains isolated in the respiratory diseases wards: resistance to amikacin was 24.5% versus 10% respectively. From 21 P. aeruginosa imipenem and multidrug resistant strains tested, 3 were probably producing of metallo-beta-lactamase. S. aureus showed 47.1% oxacillin resistance, 38.9% resistance to gentamicin and ciprofloxacin and 27.7% to erythromycin. All S.aureus strains were susceptible to linezolid, teicoplanin and vancomycin. The resistance of Enterobacteriaceae strains was high to ampicillin (80.0%), amoxicillin/clavulanic acid and trimethoprim/sulfamethoxazole e(57.6%); the lowest resistance rate was to cefoperazone/sulbactam (7.7%) and to imipenem and ciprofloxacin (10.8%).
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacteria/drug effects ; Bacteria/enzymology ; Bacteria/isolation & purification ; Drug Resistance, Multiple, Bacterial ; Enterobacteriaceae/drug effects ; Enterobacteriaceae/isolation & purification ; Humans ; Internal Medicine ; Microbial Sensitivity Tests ; Pleural Effusion/microbiology ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/isolation & purification ; Retrospective Studies ; Romania ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/isolation & purification ; Surgery Department, Hospital ; beta-Lactamases/biosynthesis
Chemical Substances	Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
Language	Romanian
Publishing date	2006-04
Publishing country	Romania
Document type	English Abstract ; Journal Article ; Multicenter Study
ZDB-ID	2064988-5
ISSN	2247-059X ; 2067-2993 ; 1223-3056
ISSN (online)	2247-059X
ISSN	2067-2993 ; 1223-3056
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Article ; Online: Nasopharyngeal carriage of Streptococcus pneumoniae in Romanian children before the introduction of the pneumococcal conjugated vaccination into the national immunization programme: a national, multi-centre, cross-sectional observational study.

Luminos, Monica / Dorobat, Olga / Jugulete, George / Popescu, Gabriel Adrian / Florea, Dragos / Draganescu, Anca / Cercel, Anca Streinu / Rafila, Alexandru

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

2014 Volume 29, Page(s) 169–173

Abstract: Objectives: We analysed the distribution of vaccine and non-vaccine Streptococcus pneumoniae serotypes and the antimicrobial susceptibility of pneumococcal strains isolated from healthy Romanian children.: Methods: A multi-centre cross-sectional ... ...

Abstract	Objectives: We analysed the distribution of vaccine and non-vaccine Streptococcus pneumoniae serotypes and the antimicrobial susceptibility of pneumococcal strains isolated from healthy Romanian children. Methods: A multi-centre cross-sectional study was performed in four counties to evaluate carried strains of S. pneumoniae isolated from 2000 children aged 0-5 years. Results: S. pneumoniae carriage was detected in 25.25% of the tested children. Carriage increased from 16.7% among infants to 29.4% in 3-5-year-old children (p<0.0001). The proportions of the serotypes included in pneumococcal conjugate vaccines PCV7, PCV10, and PCV13 among our isolates were 39.9%, 40.1%, and 58.7%, respectively. Erythromycin resistance was 72.5%, and it was significantly lower in non-vaccine serotypes compared with PCV13 serotypes: 57.3% versus 83.6% (p<10(-7)). Penicillin minimum inhibitory concentrations (MICs) >0.064mg/l were recorded in 71.6%, but the penicillin MIC was >2mg/l for only 8.4% of tested isolates. Conclusions: In Romanian children, the majority of carried S. pneumoniae isolates are vaccine serotypes. The isolates with MICs defining macrolide resistance were very frequent, as well as the isolates with MICs defining penicillin resistance in the case of meningitis or penicillin dose-dependent susceptibility for other infections, mainly for the strains belonging to PCV13 serotypes. The implementation of PCV13 within the Romanian national immunization programme could reduce the circulation of these strains with higher macrolide and/or penicillin MICs.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Child, Preschool ; Cross-Sectional Studies ; Drug Resistance, Bacterial ; Erythromycin ; Humans ; Immunization Programs ; Infant ; Infant, Newborn ; Macrolides/pharmacology ; Nasopharynx/microbiology ; Penicillin Resistance ; Penicillins/pharmacology ; Pneumococcal Vaccines ; Romania ; Serogroup ; Streptococcus pneumoniae/classification ; Streptococcus pneumoniae/drug effects ; Streptococcus pneumoniae/isolation & purification ; Vaccination ; Vaccines, Conjugate
Chemical Substances	Anti-Bacterial Agents ; Macrolides ; Penicillins ; Pneumococcal Vaccines ; Vaccines, Conjugate ; Erythromycin (63937KV33D)
Language	English
Publishing date	2014-12
Publishing country	Canada
Document type	Journal Article ; Multicenter Study ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2014.08.020
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Article: Rezistenţa la antibiotice a cocilor Gram pozitivi izolaţi în 2008.

Dorobăţ, Olga Mihaela / Bădicuţ, Ioana / Tălăpan, Daniela / Tenea, Cristina / Rafila, Alexandru

Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990)

2010 Volume 55, Issue 2, Page(s) 83–92

Abstract: Objective: Antibiotic resistance evaluation of Gram-positive cocci isolated in 2008.: Material and methods: Antibiotic susceptibility testing was performed for 1044 strains: 610 Staphylococcus aureus (352 from patients, 258 from carriers), 203 ... ...

Title translation	Antibiotic resistance of Gram-positive cocci isolated in 2008.
Abstract	Objective: Antibiotic resistance evaluation of Gram-positive cocci isolated in 2008. Material and methods: Antibiotic susceptibility testing was performed for 1044 strains: 610 Staphylococcus aureus (352 from patients, 258 from carriers), 203 Streptococcus pneumoniae (53 from patients, 150 from carriers), 144 Enterococcus faecalis. 57 Enterococcus faecium and 30 Streptococcus spp. using automatic systems Vitek 2 Compact. MicroScan, disc diffusion method and Etest according to 2008 CLSI. A number of 497 Streptococcus pyogenes strains were tested for eritromycin resistance. Results: There were 33.2% MRSA for strains isolated from patients and 30.0% from carriers. From MRSA strains. 35.5% were resistant to gentamicin. 33.6% to ciprofloxacin, 74.3% to erythromycin and 30.5% to rifampin. There were no S. aureus strain resistant to vancomycin and linezolid. S. aureus strains isolated from wounds were more resistant to erythromycin (43.9%) than the strains isolated from systemic infections (12.1%). From 11 S. pneumoniae strains isolated from meningitis, 4 were resistant to penicillin. Neither S. pneumoniae strain isolated from other infections, nor those from carriers had MIC to penicillin more than 4 microg/ml. S. pneumoniae strains isolated from carriers were more resistant to erythromycin. clindamycin and tetracycline than the strains isolated from patients (66.7%, 54.1%, 54.2% vs. 27.4%, 22.6%, 33.9%). E. faecium was 95.9% resistant to penicillin, 90.2% to ampicillin, 64.7% to gentamicin, 72.0% to streptomycin and 78.4% to ciprofloxacin. F. faecalis was less resistant than E. faecium at most of the antibiotics: 32.4% to gentamicin, 59.6% to streptomycin, 28.5% to ciprofloxacin. Viridans group Streptococci, all isolated from blood culture were 92% susceptible to penicillin and ampicillin. To erythromycin, 12% of viridians group Streptococci were resistant. S. pyogenes resistance to eritromycin was 5.8%. Conclusions: S. aureus strains showed a relatively high level of resistance to oxacillin (33.2%) and resistance in the same time to several antibiotics. S. pneumoniae can not be considered resistant to penicillin administrated parenteral, with exception of the strains isolated from meningitis. E. faecium had a higher resistance rate than E. faecalis.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Ciprofloxacin/pharmacology ; Drug Resistance, Microbial ; Enterococcus faecalis/drug effects ; Enterococcus faecium/drug effects ; Erythromycin/pharmacology ; Gentamicins/pharmacology ; Gram-Positive Bacterial Infections/drug therapy ; Gram-Positive Bacterial Infections/epidemiology ; Gram-Positive Bacterial Infections/microbiology ; Gram-Positive Bacterial Infections/prevention & control ; Gram-Positive Cocci/drug effects ; Gram-Positive Cocci/isolation & purification ; Hospitals, Isolation/statistics & numerical data ; Hospitals, University ; Humans ; Microbial Sensitivity Tests ; Penicillins/pharmacology ; Rifampin/pharmacology ; Romania/epidemiology ; Staphylococcus aureus/drug effects ; Streptococcus pneumoniae/drug effects ; Streptococcus pyogenes/drug effects
Chemical Substances	Anti-Bacterial Agents ; Gentamicins ; Penicillins ; Ciprofloxacin (5E8K9I0O4U) ; Erythromycin (63937KV33D) ; Rifampin (VJT6J7R4TR)
Language	Romanian
Publishing date	2010-04
Publishing country	Romania
Document type	English Abstract ; Journal Article
ZDB-ID	193174-x
ISSN	1220-3696
ISSN	1220-3696
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Article ; Online: Microbiologic Safety of the Transareolar Approach in Breast Augmentation.

Jianu, Dana Mihaela / Săndulescu, Oana / Streinu-Cercel, Anca / Berciu, Ioana / Blidaru, Alexandru / Filipescu, Maria / Vartic, Mihaela / Cobani, Oltjon / Jianu, Ștefan Adrian / Tălăpan, Daniela / Dorobăț, Olga / Stăniceanu, Florica / Streinu-Cercel, Adrian

Aesthetic surgery journal

2016 Volume 36, Issue 1, Page(s) 51–57

Abstract: Background: In aesthetic breast augmentation, especially by the transareolar approach, there is increasing concern regarding the occurrence of capsular contracture and its potential correlation with intraoperative implant contamination from putative ... ...

Abstract	Background: In aesthetic breast augmentation, especially by the transareolar approach, there is increasing concern regarding the occurrence of capsular contracture and its potential correlation with intraoperative implant contamination from putative endogenous breast flora of the nipple and lactiferous ducts. However, detectable bacteria cannot be considered synonymous with established resident microflora. Objectives: The authors sought to elucidate the existence of endogenous breast flora and assess the microbiologic safety of transareolar breast augmentation. Methods: In this prospective study (BREAST-MF), the authors collected microbiologic samples from the breast skin, ductal tissue, and parenchyma of 39 consecutive female patients who underwent breast procedures in a plastic surgery clinic. Swabs collected pre-, intra-, and postoperatively were processed for bacterial and fungal growth. Positive cultures underwent identification through VITEK and MALDI-TOF, as well as antimicrobial susceptibility testing. Results: Staphylococcus species accounted for 95 of 106 (89.6%) positive results from native breast skin, 15 of 18 (83.3%) positive results from decontaminated breast skin, and 4 of 4 (100%) positive results from the breast parenchyma. Methicillin resistance was present in 26.4% of S. epidermidis, 25.3% of S. hominis, and 71.4% of S. haemolyticus strains. Conclusions: During transareolar breast augmentation, in the nipple-areola region it is more likely to find bacteria populating the skin, rather than endogenous breast flora, as previously considered. Appropriate preoperative decontamination is essential for minimizing the risk of postoperative infections. LEVEL OF EVIDENCE 3: Risk.
MeSH term(s)	Adult ; Aged ; Breast/microbiology ; Breast Implants ; Female ; Humans ; Mammaplasty ; Middle Aged ; Nipples/microbiology ; Postoperative Complications/microbiology ; Prospective Studies ; Staphylococcus/isolation & purification
Language	English
Publishing date	2016-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2087022-X
ISSN	1527-330X ; 1090-820X ; 1084-0761
ISSN (online)	1527-330X
ISSN	1090-820X ; 1084-0761
DOI	10.1093/asj/sjv106
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Article ; Online: Comparative evaluation of aggressiveness traits in staphylococcal strains from severe infections versus nasopharyngeal carriage.

Săndulescu, Oana / Bleotu, Coralia / Matei, Lilia / Streinu-Cercel, Anca / Oprea, Mihaela / Drăgulescu, Elena Carmina / Chifiriuc, Mariana Carmen / Rafila, Alexandru / Pirici, Daniel / Tălăpan, Daniela / Dorobăţ, Olga Mihaela / Neguţ, Alina Cristina / Oţelea, Dan / Berciu, Ioana / Ion, Daniela Adriana / Codiţă, Irina / Calistru, Petre Iacob / Streinu-Cercel, Adrian

Microbial pathogenesis

2017 Volume 102, Page(s) 45–53

Abstract: Despite their commensal status, staphylococci can become problematic pathogens expressing multiple and redundant virulence factors. This study aimed to evaluate aggressiveness markers comparatively in staphylococcal strains isolated from severe ... ...

Abstract	Despite their commensal status, staphylococci can become problematic pathogens expressing multiple and redundant virulence factors. This study aimed to evaluate aggressiveness markers comparatively in staphylococcal strains isolated from severe infections versus asymptomatic carriage in order to identify clinically relevant bacterial traits that could easily be detected in clinical practice and could be suggestive for particular host-pathogen interactions such as cyto-adhesion or biofilm formation, ultimately orienting the clinical decision-making process. We have used in vitro phenotypic methods to assess adhesion to and invasion of eukaryotic cells, biofilm development, and expression of soluble virulence factors in 92 Staphylococcus spp. strains. The adhesion index, invasion capacity, biofilm formation and expression of soluble factors did not differ significantly between clinical and commensal strains. The major bacterial traits we found to be significantly more prevalent in clinical staphylococci were the aggregative adhesion pattern (P = 0.012), cluster adhesion (P = 0.001) and tetrad morphology (P = 0.018). The aggregative adhesion pattern was correlated with higher cyto-adhesion (P < 0.001), higher invasion capacity (P = 0.003) and lower Carmeli scores (P = 0.002). Three major bacterial traits, namely tetrad morphology, aggregative adhesion pattern, and resistance to methicillin (acronym: TAM), can be used to compute an aggressiveness score (SAS) predictive of the staphylococcal strain's virulence and capacity to initiate and develop a biofilm-driven chronic infectious process versus a fulminant acute infection, in a susceptible host.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anti-Bacterial Agents/pharmacology ; Bacterial Adhesion ; Biofilms ; Carrier State ; Cell Line ; Child ; Child, Preschool ; Comorbidity ; Female ; Genetic Variation ; Humans ; Infant ; Infant, Newborn ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Nasopharynx/microbiology ; Quantitative Trait, Heritable ; Severity of Illness Index ; Staphylococcal Infections/diagnosis ; Staphylococcal Infections/microbiology ; Staphylococcus/classification ; Staphylococcus/drug effects ; Staphylococcus/pathogenicity ; Staphylococcus/physiology ; Virulence ; Virulence Factors ; Young Adult
Chemical Substances	Anti-Bacterial Agents ; Virulence Factors
Language	English
Publishing date	2017-01
Publishing country	England
Document type	Journal Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2016.11.006
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Article ; Online: Bacteriophage-driven inhibition of biofilm formation in Staphylococcus strains from patients attending a Romanian reference center for infectious diseases.

Neguţ, Alina Cristina / Chifiriuc, Mariana-Carmen / Săndulescu, Oana / Streinu-Cercel, Anca / Oprea, Mihaela / Drăgulescu, Elena Carmina / Gheorghe, Irina / Berciu, Ioana / Coralia, Bleotu / Popa, Marcela / Oţelea, Dan / Tălăpan, Daniela / Dorobăţ, Olga / Codiţă, Irina / Popa, Mircea Ioan / Streinu-Cercel, Adrian

FEMS microbiology letters

2016 Volume 363, Issue 18

Abstract: The increasing burden of invasive biofilm-related staphylococcal infections has led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter a biofilm through several mechanisms, including induction of ... ...

Abstract	The increasing burden of invasive biofilm-related staphylococcal infections has led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter a biofilm through several mechanisms, including induction of depolymerizing enzymes and lysis of persistent bacteria. We have assessed the influence of commercially available bacteriophage cocktails on Staphylococcus spp. clinical strains viability and biofilm formation. We analyzed 83 staphylococcal strains from patients consecutively admitted to a Romanian infection reference center from October 2014 through May 2015; the strains were characterized by phenotypic and genetic tools for their resistance and virulence features and for their phyliation. Experiments were performed in triplicate. Methicillin-susceptible strains were significantly more susceptible to all tested phages: 1.7-fold higher susceptibility for PYO, 1.4-fold for INTESTI, 2.9-fold for PHAGYO, 2.7-fold for PHAGESTI and 3.9-fold for STAPHYLOCOCCAL; t030 strains were significantly more susceptible to PYO and INTESTI compared with t127 strains. We identified a significant decrease in biofilm formation in the presence of both low and high PYO and INTESTI concentrations (P < 0.001). In conclusion, Staphylococcus strains from Romania displayed fairly good susceptibility to commercially available bacteriophages. We have also ascertained there is phage-driven in vitro inhibition of biofilm formation, the results potentially impacting prevention of prosthetic infections.
Language	English
Publishing date	2016-09
Publishing country	England
Document type	Journal Article
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1093/femsle/fnw193
Database	MEDical Literature Analysis and Retrieval System OnLINE

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