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Article ; Online: A first complete catalog of highly expressed genes in eight chicken tissues reveals uncharacterized gene families specific for the chicken testis.

Piégu, Benoit / Lefort, Gaëlle / Douet, Cécile / Milhes, Marine / Jacques, Aurore / Lareyre, Jean-Jacques / Monget, Philippe / Fouchécourt, Sophie

Physiological genomics

2024

Abstract: Based on next-generation sequencing, we established a repertoire of differentially overexpressed genes (DoEG) in eight adult chicken tissues: testis, brain, lung, liver, kidney, muscle, heart, and intestine. With 4499 DoEG, the testis had the highest ... ...

Abstract	Based on next-generation sequencing, we established a repertoire of differentially overexpressed genes (DoEG) in eight adult chicken tissues: testis, brain, lung, liver, kidney, muscle, heart, and intestine. With 4499 DoEG, the testis had the highest number and proportion of DoEG compared with the seven somatic tissues. The testis DoEG set included the highest proportion of long noncoding RNAs (lncRNAs; 1851, representing 32% of the lncRNA genes in the whole genome) and the highest proportion of protein-coding genes (2648, representing 14.7% of the protein-coding genes in the whole genome). The main significantly enriched Gene Ontology terms related to the protein-coding genes are "reproductive process," "tubulin binding," "microtubule cytoskeleton." By using real-time quantitative reverse transcription polymerase chain reaction, we confirmed the overexpression of genes that encode proteins already described in chicken sperm (such as
Language	English
Publishing date	2024-03-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	2038823-8
ISSN	1531-2267 ; 1094-8341
ISSN (online)	1531-2267
ISSN	1094-8341
DOI	10.1152/physiolgenomics.00151.2023
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Article: Expanding duplication of the testis PHD Finger Protein 7 (PHF7) gene in the chicken genome

Fouchécourt, Sophie / Fillon, Valérie / Marrauld, Christelle / Callot, Caroline / Ronsin, Sarah / Picolo, Floriane / Douet, Cécile / Piégu, Benoit / Monget, Philippe

Genomics. 2022 July, v. 114, no. 4

2022

Abstract: Gene duplications increase genetic and phenotypic diversity and occur in complex genomic regions that are still difficult to sequence and assemble. PHD Finger Protein 7 (PHF7) acts during spermiogenesis for histone-to-histone protamine exchange and is a ... ...

Abstract	Gene duplications increase genetic and phenotypic diversity and occur in complex genomic regions that are still difficult to sequence and assemble. PHD Finger Protein 7 (PHF7) acts during spermiogenesis for histone-to-histone protamine exchange and is a determinant of male fertility in Drosophila and the mouse. We aimed to explore and characterise in the chicken genome the expanding family of the numerous orthologues of the unique mouse Phf7 gene (highly expressed in the testis), observing the fact that this information is unclear and/or variable according to the versions of databases. We validated nine primer pairs by in silico PCR for their use in screening the chicken bacterial artificial chromosome (BAC) library to produce BAC-derived probes to detect and localise PHF7-like loci by fluorescence in situ hybridisation (FISH). We selected nine BAC that highlighted nine chromosomal regions for a total of 10 distinct PHF7-like loci on five Gallus gallus chromosomes: Chr1 (three loci), Chr2 (two loci), Chr12 (one locus), Chr19 (one locus) and ChrZ (three loci). We sequenced the corresponding BAC by using high-performance PacBio technology. After assembly, we performed annotation with the FGENESH program: there were a total of 116 peptides, including 39 PHF7-like proteins identified by BLASTP. These proteins share a common exon-intron core structure of 8–11 exons. Phylogeny revealed that the duplications occurred first between chromosomal regions and then inside each region. There are other duplicated genes in the identified BAC sequences, suggesting that these genomic regions exhibit a high rate of tandem duplication. We showed that the PHF7 gene, which is highly expressed in the rooster testis, is a highly duplicated gene family in the chicken genome, and this phenomenon probably concerns other bird species.
Keywords	Drosophila ; Gallus gallus ; bacterial artificial chromosomes ; computer simulation ; exons ; fish ; fluorescence ; gene duplication ; genomics ; hybridization ; loci ; male fertility ; mice ; peptides ; phenotypic variation ; phylogeny ; roosters ; spermiogenesis ; testes
Language	English
Dates of publication	2022-07
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	356334-0
ISSN	1089-8646 ; 0888-7543
ISSN (online)	1089-8646
ISSN	0888-7543
DOI	10.1016/j.ygeno.2022.110411
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Article ; Online: Expanding duplication of the testis PHD Finger Protein 7 (PHF7) gene in the chicken genome.

Fouchécourt, Sophie / Fillon, Valérie / Marrauld, Christelle / Callot, Caroline / Ronsin, Sarah / Picolo, Floriane / Douet, Cécile / Piégu, Benoit / Monget, Philippe

Genomics

2022 Volume 114, Issue 4, Page(s) 110411

Abstract	Gene duplications increase genetic and phenotypic diversity and occur in complex genomic regions that are still difficult to sequence and assemble. PHD Finger Protein 7 (PHF7) acts during spermiogenesis for histone-to-histone protamine exchange and is a determinant of male fertility in Drosophila and the mouse. We aimed to explore and characterise in the chicken genome the expanding family of the numerous orthologues of the unique mouse Phf7 gene (highly expressed in the testis), observing the fact that this information is unclear and/or variable according to the versions of databases. We validated nine primer pairs by in silico PCR for their use in screening the chicken bacterial artificial chromosome (BAC) library to produce BAC-derived probes to detect and localise PHF7-like loci by fluorescence in situ hybridisation (FISH). We selected nine BAC that highlighted nine chromosomal regions for a total of 10 distinct PHF7-like loci on five Gallus gallus chromosomes: Chr1 (three loci), Chr2 (two loci), Chr12 (one locus), Chr19 (one locus) and ChrZ (three loci). We sequenced the corresponding BAC by using high-performance PacBio technology. After assembly, we performed annotation with the FGENESH program: there were a total of 116 peptides, including 39 PHF7-like proteins identified by BLASTP. These proteins share a common exon-intron core structure of 8-11 exons. Phylogeny revealed that the duplications occurred first between chromosomal regions and then inside each region. There are other duplicated genes in the identified BAC sequences, suggesting that these genomic regions exhibit a high rate of tandem duplication. We showed that the PHF7 gene, which is highly expressed in the rooster testis, is a highly duplicated gene family in the chicken genome, and this phenomenon probably concerns other bird species.
MeSH term(s)	Animals ; Chickens/genetics ; Chickens/metabolism ; Chromosomes, Artificial, Bacterial/metabolism ; Gene Duplication ; Genome ; Histones/metabolism ; Male ; Mice ; PHD Zinc Fingers ; Testis/metabolism
Chemical Substances	Histones
Language	English
Publishing date	2022-06-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	356334-0
ISSN	1089-8646 ; 0888-7543
ISSN (online)	1089-8646
ISSN	0888-7543
DOI	10.1016/j.ygeno.2022.110411
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Article ; Online: Effect of cumulus cell removal and sperm pre-incubation with progesterone on in vitro fertilization of equine gametes in the presence of oviductal fluid or cells.

Moros-Nicolás, Carla / Douet, Cécile / Reigner, Fabrice / Goudet, Ghylène

Reproduction in domestic animals = Zuchthygiene

2019 Volume 54, Issue 8, Page(s) 1095–1103

Abstract: In spite of many attempts to establish an in vitro fertilization (IVF) technique in the equine, no efficient conventional IVF technique is available. The presence of oviductal fluid or oviductal cells during IVF helps to improve embryo production in ... ...

Abstract	In spite of many attempts to establish an in vitro fertilization (IVF) technique in the equine, no efficient conventional IVF technique is available. The presence of oviductal fluid or oviductal cells during IVF helps to improve embryo production in vitro but is not sufficient to reach high fertilization rates. Thus, our aim was to perform equine IVF either after sperm pre-incubation with oviductal fluid or in the presence of oviductal cells, and to evaluate the effect of cumulus removal from the oocyte or sperm pre-incubation with progesterone. In experiments 1 and 2, IVF was performed in the presence of porcine oviduct epithelial cells. The removal of cumulus cells from equine oocytes after in vitro maturation tended to increase the percentage of fertilization when fresh sperm was used (1/33 vs. 4/31, p > 0.05) but had no effect when frozen sperm was used (1/32 vs. 1/32). Equine sperm pre-incubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/14 vs. 2/18 for fresh, 1/29 vs. 1/25 for frozen). In experiments 3 and 4, IVF was performed after pre-incubation of sperm with porcine oviductal fluid. The removal of cumulus cells tended to increase the percentage of fertilization when fresh sperm was used (1/24 vs. 3/26, p > 0.05). Sperm pre-incubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/39 vs. 2/36 for fresh, 2/37 vs. 1/46 for frozen), but two 3-4 cell stage zygotes were obtained with fresh sperm pre-incubated with progesterone. This is an encouraging result for the setting up of an efficient IVF procedure in equine.
MeSH term(s)	Animals ; Body Fluids ; Cumulus Cells ; Fallopian Tubes/cytology ; Female ; Fertilization in Vitro/methods ; Fertilization in Vitro/veterinary ; Horses ; In Vitro Oocyte Maturation Techniques/methods ; In Vitro Oocyte Maturation Techniques/veterinary ; Male ; Oocytes/physiology ; Progesterone/pharmacology ; Spermatozoa/drug effects ; Spermatozoa/physiology ; Swine
Chemical Substances	Progesterone (4G7DS2Q64Y)
Language	English
Publishing date	2019-06-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1015187-4
ISSN	1439-0531 ; 0936-6768
ISSN (online)	1439-0531
ISSN	0936-6768
DOI	10.1111/rda.13479
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Article: First attempts for vitrification of immature oocytes in donkey (Equus asinus): Comparison of two vitrification methods

Douet, Cécile / Barrière, Philippe / Blard, Thierry / Deleuze, Stefan / Goudet, Ghylène / Reigner, Fabrice

Theriogenology. 2019 Mar. 01, v. 126

2019

Abstract: Most wild donkey breeds are severely threatened by poaching for meat, habitat loss, and competition with livestock for food resources. Moreover, due to the mechanization in agriculture and in transport, most domestic donkey breeds are at risk of ... ...

Abstract	Most wild donkey breeds are severely threatened by poaching for meat, habitat loss, and competition with livestock for food resources. Moreover, due to the mechanization in agriculture and in transport, most domestic donkey breeds are at risk of extinction. Considering the importance of biodiversity and preservation of genetic resources, the creation of genetic banks for endangered donkey breeds is urgently needed. Cryopreservation of immature jennies oocytes would be an efficient tool to allow storage of female genetics. The aim of the present study was to establish conditions for immature donkey oocyte vitrification, using equine oocytes as a control. Asine and equine immature cumulus-oocyte complexes were collected by transvaginal ultrasound-guided follicular aspiration and flushed to obtain oocytes surrounded by only corona radiata. Oocytes were vitrified after exposure to increasing concentrations of dimethyl sulfoxide, ethylene glycol and sucrose as cryoprotectants in a solution of INRA-FreezeTM medium or TCM199-Hepes supplemented with bovine serum albumin. Oocytes were warmed in decreasing concentrations of sucrose and processed for in vitro maturation. The recovery rate was 48% for jennies oocytes (4.8 oocyte per female) and 42% for mares oocytes (3.5 oocyte per female). When oocytes were exposed to cryoprotectants in INRA-FreezeTM medium none of the jennies re-warmed oocytes matured, whereas 24% of the mares re-warmed oocytes reached metaphase II after in vitro maturation. When oocytes were exposed to cryoprotectants in TCM199-Hepes-BSA medium, 33% of the jennies re-warmed oocytes matured. In conclusion, we developed a method for the vitrification of immature oocytes from jennies that allows in vitro maturation of the vitrified-warmed asine oocytes. Their competence for fertilization and development has to be ascertain.
Keywords	agricultural mechanization ; asses ; biodiversity ; bovine serum albumin ; breeds ; cryoprotectants ; dimethyl sulfoxide ; Equus asinus ; ethylene glycol ; extinction ; habitat destruction ; mares ; meat ; metaphase ; oocyte vitrification ; oocytes ; risk ; sucrose ; ultrasonography ; vitrification
Language	English
Dates of publication	2019-0301
Size	p. 261-265.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2018.12.030
Database	NAL-Catalogue (AGRICOLA)

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Article: Effect of cumulus cell removal and sperm pre‐incubation with progesterone on in vitro fertilization of equine gametes in the presence of oviductal fluid or cells

Moros‐Nicolás, Carla / Douet, Cécile / Reigner, Fabrice / Goudet, Ghylène

Reproduction in domestic animals. 2019 Aug., v. 54, no. 8

2019

Abstract	In spite of many attempts to establish an in vitro fertilization (IVF) technique in the equine, no efficient conventional IVF technique is available. The presence of oviductal fluid or oviductal cells during IVF helps to improve embryo production in vitro but is not sufficient to reach high fertilization rates. Thus, our aim was to perform equine IVF either after sperm pre‐incubation with oviductal fluid or in the presence of oviductal cells, and to evaluate the effect of cumulus removal from the oocyte or sperm pre‐incubation with progesterone. In experiments 1 and 2, IVF was performed in the presence of porcine oviduct epithelial cells. The removal of cumulus cells from equine oocytes after in vitro maturation tended to increase the percentage of fertilization when fresh sperm was used (1/33 vs. 4/31, p > 0.05) but had no effect when frozen sperm was used (1/32 vs. 1/32). Equine sperm pre‐incubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/14 vs. 2/18 for fresh, 1/29 vs. 1/25 for frozen). In experiments 3 and 4, IVF was performed after pre‐incubation of sperm with porcine oviductal fluid. The removal of cumulus cells tended to increase the percentage of fertilization when fresh sperm was used (1/24 vs. 3/26, p > 0.05). Sperm pre‐incubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/39 vs. 2/36 for fresh, 2/37 vs. 1/46 for frozen), but two 3–4 cell stage zygotes were obtained with fresh sperm pre‐incubated with progesterone. This is an encouraging result for the setting up of an efficient IVF procedure in equine.
Keywords	epithelial cells ; horses ; in vitro fertilization ; oocytes ; oviducts ; progesterone ; spermatozoa ; swine ; zygote
Language	English
Dates of publication	2019-08
Size	p. 1095-1103.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1015187-4
ISSN	1439-0531 ; 0936-6768
ISSN (online)	1439-0531
ISSN	0936-6768
DOI	10.1111/rda.13479
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: First attempts for vitrification of immature oocytes in donkey (Equus asinus): Comparison of two vitrification methods.

Douet, Cécile / Reigner, Fabrice / Barrière, Philippe / Blard, Thierry / Deleuze, Stefan / Goudet, Ghylène

Theriogenology

2018 Volume 126, Page(s) 261–265

Abstract	Most wild donkey breeds are severely threatened by poaching for meat, habitat loss, and competition with livestock for food resources. Moreover, due to the mechanization in agriculture and in transport, most domestic donkey breeds are at risk of extinction. Considering the importance of biodiversity and preservation of genetic resources, the creation of genetic banks for endangered donkey breeds is urgently needed. Cryopreservation of immature jennies oocytes would be an efficient tool to allow storage of female genetics. The aim of the present study was to establish conditions for immature donkey oocyte vitrification, using equine oocytes as a control. Asine and equine immature cumulus-oocyte complexes were collected by transvaginal ultrasound-guided follicular aspiration and flushed to obtain oocytes surrounded by only corona radiata. Oocytes were vitrified after exposure to increasing concentrations of dimethyl sulfoxide, ethylene glycol and sucrose as cryoprotectants in a solution of INRA-Freeze™ medium or TCM199-Hepes supplemented with bovine serum albumin. Oocytes were warmed in decreasing concentrations of sucrose and processed for in vitro maturation. The recovery rate was 48% for jennies oocytes (4.8 oocyte per female) and 42% for mares oocytes (3.5 oocyte per female). When oocytes were exposed to cryoprotectants in INRA-Freeze™ medium none of the jennies re-warmed oocytes matured, whereas 24% of the mares re-warmed oocytes reached metaphase II after in vitro maturation. When oocytes were exposed to cryoprotectants in TCM199-Hepes-BSA medium, 33% of the jennies re-warmed oocytes matured. In conclusion, we developed a method for the vitrification of immature oocytes from jennies that allows in vitro maturation of the vitrified-warmed asine oocytes. Their competence for fertilization and development has to be ascertain.
MeSH term(s)	Animals ; Biodiversity ; Biological Specimen Banks ; Cryopreservation/methods ; Cryopreservation/veterinary ; Endangered Species ; Equidae ; Horses ; Oocyte Retrieval/veterinary ; Oocytes ; Ovulation Induction/methods ; Ovulation Induction/veterinary ; Vitrification
Language	English
Publishing date	2018-12-18
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2018.12.030
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Article ; Online: Influence of transvaginal ultrasound-guided follicular punctures in the mare on heart rate, respiratory rate, facial expression changes, and salivary cortisol as pain scoring.

Diego, Rodrigo / Douet, Cécile / Reigner, Fabrice / Blard, Thierry / Cognié, Juliette / Deleuze, Stefan / Goudet, Ghylène

Theriogenology

2016 Volume 86, Issue 7, Page(s) 1757–1763

Abstract: Transvaginal ultrasound-guided follicular punctures are widely used in the mare for diagnosis, research, and commercial applications. The objective of our study was to determine their influence on pain, stress, and well-being in the mare, by evaluating ... ...

Abstract	Transvaginal ultrasound-guided follicular punctures are widely used in the mare for diagnosis, research, and commercial applications. The objective of our study was to determine their influence on pain, stress, and well-being in the mare, by evaluating heart rate, breath rate, facial expression changes, and salivary cortisol before, during, and after puncture. For this experiment, 21 pony mares were used. Transvaginal ultrasound-guided aspirations were performed on 11 mares. After injections for sedation, analgesia, and antispasmodia, the follicles from both ovaries were aspirated with a needle introduced through the vagina wall into the ovary. In the control group, 10 mares underwent similar treatments and injections, but no follicular aspiration. Along the session, heart rate and breath rate were evaluated by a trained veterinarian, ears position, eyelid closure, and contraction of facial muscles were evaluated, and salivary samples were taken for evaluation of cortisol concentration. A significant relaxation was observed after sedative injection in the punctured and control mares, according to ear position, eyelid closure, and contraction of facial muscles, but no difference between punctured and control animals was recorded. No significant modification of salivary cortisol concentration during puncture and no difference between punctured and control mares at any time were observed. No significant modification of the breath rate was observed along the procedure for the punctured and the control mares. Heart rate increased significantly but transiently when the needle was introduced in the ovary and was significantly higher at that time for the punctured mares than that for control mares. None of the other investigated parameters were affected at that time, suggesting discomfort is minimal and transient. Improving analgesia, e.g., through a multimodal approach, during that possibly more sensitive step could be recommended. The evaluation of facial expression changes and heart rate is easy-to-use and accurate tools to evaluate pain and well-being of the mare.
MeSH term(s)	Animals ; Facial Expression ; Female ; Heart Rate/physiology ; Horse Diseases/diagnosis ; Horses ; Hydrocortisone/chemistry ; Ovarian Follicle/surgery ; Pain/etiology ; Pain/veterinary ; Pain Measurement/veterinary ; Punctures/adverse effects ; Punctures/veterinary ; Respiratory Physiological Phenomena ; Saliva/chemistry ; Ultrasonography/methods ; Ultrasonography/veterinary
Chemical Substances	Hydrocortisone (WI4X0X7BPJ)
Language	English
Publishing date	2016-10-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2016.05.040
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Article: Ovum Pick Up and In Vitro Maturation of Jennies Oocytes Toward the Setting Up of Efficient In Vitro Fertilization and In Vitro Embryos Culture Procedures in Donkey (Equus asinus)

Deleuze, Stefan / Barrière, Philippe / Blard, Thierry / Couty, Isabelle / Douet, Cécile / Goudet, Ghylène / Magistrini, Michèle / Moros-Nicolás, Carla / Reigner, Fabrice

Journal of equine veterinary science. 2018 June, v. 65

2018

Abstract: Most wild and domestic donkey breeds are currently endangered or threatened. Their preservation includes the creation of a Genome Resource Bank. Embryo cryopreservation allows preservation of genetics from both male and female and is the fastest method ... ...

Abstract	Most wild and domestic donkey breeds are currently endangered or threatened. Their preservation includes the creation of a Genome Resource Bank. Embryo cryopreservation allows preservation of genetics from both male and female and is the fastest method to restore a breed. Embryo production in vivo is limited in equids. We recently established a technique of ovum pick up (OPU) in the donkey. Conditions of in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture of zygotes have been evaluated. Equine abattoir–derived oocytes were used as controls. Donkey cumulus-oocyte complexes (COCs) collected by OPU were matured in vitro in TCM199 with fetal calf serum and epidermal growth factor for 24, 30, 34, or 38 hours. Forty-four percent were in metaphase 2 after 34 hours. In our conditions, IVM of donkey oocytes was slower than that of equine oocytes and the optimal duration for donkey oocytes IVM may be 34 hours. Oocytes we co-incubated with frozen-thawed donkey semen treated with procaine for 18 hours and cultured for 30 hours in a Dulbecco Modified Eagle Medium-F12-based medium. Only 15% of jennies oocytes contained 2 pronuclei after co-incubation, and none of them developed further after 48 hours after IVF. Treatment of donkey sperm with procaine may not be efficient for IVF. Some parthenogenetic activation occurred. In conclusion, we confirm that our conditions for OPU in jennies yielded high recovery rates that improved with operator experience. Maturation rates of 44% can be achieved using the IVM medium routinely used for equine oocytes in our lab. Further studies are in progress to establish efficient conditions for IVF and development of donkey zygotes.
Keywords	asses ; breeds ; cryopreservation ; epidermal growth factor ; Equus asinus ; females ; fetal bovine serum ; freeze-thaw cycles ; genome ; horses ; in vitro culture ; in vitro fertilization ; males ; metaphase ; oocytes ; parthenogenesis ; procaine ; pronucleus ; semen ; spermatozoa ; zygote
Language	English
Dates of publication	2018-06
Size	p. 111-117.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	2102631-2
ISSN	1542-7412 ; 0737-0806
ISSN (online)	1542-7412
ISSN	0737-0806
DOI	10.1016/j.jevs.2018.03.004
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Conference proceedings ; Online: Steroidome And Metabolome Analysis In Saliva From Immature To Pubertal Gilts To Identify Potential Biomarkers Of Receptivity To Boar Effect

Goudet, Ghylène / Liere, Philippe / Pianos, Antoine / Fernandez, Neïké / Cambourg, Annie / Savoie, Jonathan / Staub, Christophe / Venturi, Eric / Douet, Cécile / Ferchaud, Stéphane / Maupertuis, Florence / Roinsard, Antoine / Boulot, Sylviane / Prunier, Armelle

2021

Abstract: Our objective was to develop alternatives to hormones for estrus synchronization in gilts. Gilts exhibit a pre-puberty period with high urinary estrone concentration during which boar exposure could induce the first ovulation. We searched for salivary ... ...

Abstract	Our objective was to develop alternatives to hormones for estrus synchronization in gilts. Gilts exhibit a pre-puberty period with high urinary estrone concentration during which boar exposure could induce the first ovulation. We searched for salivary biomarkers of this period. Urine and saliva were collected on six 140-day-old gilts until puberty for estrone assay, metabolome and steroidome analysis. We identified 23 metabolites and 28 steroids in saliva. The concentration of 8 of them showed significant variations at the pre-puberty period, they were candidate biomarkers. Saliva was collected from 30 gilts exposed to a boar and subjected to estrus detection from 150 to 175 days of age. Metabolome and steroidome analyses allowed the identification of 33 metabolites and 29 steroids in saliva. Their concentrations were not significantly different between receptive and non-receptive gilts. Thus, we could not identify salivary biomarkers of the period of receptivity to the boar effect.
Keywords	"Organics" in general
Subject code	630
Language	English
Publishing country	dk
Document type	Conference proceedings ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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In stock of ZB MED Cologne/Königswinter

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In stock of ZB MED Cologne/Königswinter

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Inter-library loan at ZB MED