Article ; Online: Determining the affinities of high-affinity antibodies using KinExA and surface plasmon resonance.
2023 Volume 15, Issue 1, Page(s) 2291209
Abstract: Accurate and efficient affinity measurement techniques are essential for the biophysical characterization of therapeutic monoclonal antibodies, one of the fastest growing drug classes. Surface plasmon resonance (SPR) is widely used for determining ... ...
| Abstract | Accurate and efficient affinity measurement techniques are essential for the biophysical characterization of therapeutic monoclonal antibodies, one of the fastest growing drug classes. Surface plasmon resonance (SPR) is widely used for determining antibody affinity, but does not perform well with extremely high affinity (low picomolar to femtomolar range) molecules. In this study, we compare the SPR-based Carterra LSA and the kinetic exclusion assay (KinExA) for measuring the affinities of 48 antibodies generated against the SARS-CoV-2 receptor-binding domain. These data reveal that high-affinity antibodies can be generated straight from selections using high-quality |
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| MeSH term(s) | Surface Plasmon Resonance/methods ; Antibodies, Monoclonal/chemistry ; Antibody Affinity |
| Chemical Substances | Antibodies, Monoclonal |
| Language | English |
| Publishing date | 2023-12-13 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2537838-7 |
| ISSN | 1942-0870 ; 1942-0870 |
| ISSN (online) | 1942-0870 |
| ISSN | 1942-0870 |
| DOI | 10.1080/19420862.2023.2291209 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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