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  1. Thesis ; Online: Consolidating concepts of technology education

    Doyle, Andrew

    From rhetoric towards a potential reality

    2020  

    Abstract: The thesis focuses on the relationship between international rhetoric and classroom realities in technology education. For some time there has been widespread recognition that the intended goals for learning in the subject area have failed to manifest in ...

    Abstract The thesis focuses on the relationship between international rhetoric and classroom realities in technology education. For some time there has been widespread recognition that the intended goals for learning in the subject area have failed to manifest in enacted practices as envisioned. As the intermediary between rhetoric and reality, the technology teachers and ways of understanding their enacted practices are the focus of this work. The thesis is based on four research articles which adopt theoretical and empirical approaches to investigating the technology teacher as mediator of enacted practice. In Article I, technology education in the Irish national context is investigated through technology teachers’ reflections on enacted practice. In response to a variety of situational- and systemic- factors which impede classroom practice being identified, Article II and III theorise approaches to investigating enacted practice in technology. In acknowledging the epistemological basis of technology as depicted in the extant literature, a reconceptualisation of how to utilise pedagogical content knowledge research in explaining enacted practice is put forward. Article IV returns to the technology teacher in a transnational context, whereby teachers from the Republic of Ireland, Sweden and New Zealand are interviewed in constructing a grounded theory of teachers’ purposes for teaching technology. The contributions of the research are twofold. Firstly, following the identification of evidence to support the existence of rhetoric-reality tensions in technology education, an ecologically situated framework of enacted practice is put forward. The framework acknowledges how subject matter is treated in technology education in striving for more comprehensive ways of investigating enacted practice. Secondly, in taking a preliminary step toward understanding enacted practices, a grounded theory of teachers’ purposes for teaching technology is put forward. This grounded theory offers a unified model for articulating the purposes of teaching technology that prevail in classroom realities today.

    Den här avhandlingen handlar om hur diskursen iteknikundervisning förhåller sig till den undervisning som bedrivs i klassrummet i en internationell kontext. Det är känt sedan tidigare att de mål som återfinns i styrdokument för teknikundervisning till stor del inte uppfylls genom rådande klassrumspraktik. I syfte att förstå bakgrunden till denna diskrepans fokuserar det här arbetet på lärarens roll i teknikundervisningen. Läraren betraktas här som den mellanhand som omvandlar retoriken till undervisningspraktik. Avhandlingen bygger på fyra forskningsartiklar som använder både teoretiska och empiriska angreppsätt för att undersöka tekniklärarens roll som förmedlare av kunskap i teknikämnet. Artikel I undersöker teknikundervisning i en irländsk kontext genom att analysera tekniklärarnas reflektioner över sin praktik i klassrummet. Studien identifierar flera faktorer som påverkar klassrumspraktiken. Artikel II och III bygger vidare på det resultat som framkom i studie I genom att teoretiskt resonera kring lämpliga metoder för att undersöka teknikämnets klassrumspraktik. Genom att använda den epistemologiska utgångspunkt för teknikundervisning som speglas i litteraturen, så utformades ett nytt sätt för att använda PCK (Pedagogical Content Knowledge, ett etablerat ramverk som beskriver lärarens kompetens) som förklaringsmodell för att beskriva klassrumspraktik. Artikel IV återvänder i en empirisk studie till tekniklärarnas roll men denna gång med ett transnationellt perspektiv i syfte att arbeta fram en grundläggande teori om lärares mål och syften med teknikundervisning. Studien bygger på intervjuer med lärare från Irland, Sverige och Nya Zealand Resultatet i denna avhandling bidrar med kunskap på två sätt För det första, efter att ha identifierat faktorer som stärker tidigare belägg att det finns spänningar mellan målen beskrivna i styrdokumenten och klassrumspraktiken, så togs det fram ett teoretiskt ramverk för forskningsstudier om teknikundervisning. Ramverket utgör en modell som på ett nytt sätt beskriver förhållandet mellan de faktorer som påverkar klassrumsundervisningen. Modellen ger möjligheter att på ett mer omfattande sätt än tidigare studera och förstå klassrumsundervisningen utifrån lärarens perspektiv. För det andra, som ett första steg mot att förstå den rådande undervisningspraktiken presenteras en grundläggande teori kring lärares syften med undervisning i teknik. Denna grundläggande teori erbjuder en sammanhållen modell för att formulera, lyfta fram och tydliggöra syften med att undervisa teknik i den verklighet som råder i klassrummen idag.

    Due to the Covid-19 pandemic - this defence will be partly conducted via Zoom. Information will be published online in advance of the defence.
    Keywords Technology education ; enacted practice ; Pedagogical Content Knowledge (PCK) ; teacher conceptions ; and ; Nature of Technology ; Educational Sciences ; Utbildningsvetenskap ; Didactics ; Didaktik ; Learning ; Lärande ; Pedagogical Work ; Pedagogiskt arbete ; Pedagogy ; Pedagogik ; covid19
    Subject code 370
    Language English
    Publisher KTH, Lärande
    Publishing country se
    Document type Thesis ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Erratum: Correction Notice: Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging.

    D Doyle, Andrew

    Bio-protocol

    2020  Volume 10, Issue 9

    Abstract: This corrects the article .]. ...

    Abstract [This corrects the article .].
    Language English
    Publishing date 2020-05-05
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.3650
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging.

    Doyle, Andrew D

    Bio-protocol

    2018  Volume 8, Issue 13

    Abstract: Rat tail collagen solutions have been used as ... ...

    Abstract Rat tail collagen solutions have been used as polymerizable
    Language English
    Publishing date 2018-08-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.2919
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: The authors reply.

    Doyle, Andrew J / Retter, Andrew

    Critical care medicine

    2021  Volume 49, Issue 10, Page(s) e1050–e1051

    Language English
    Publishing date 2021-06-21
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 197890-1
    ISSN 1530-0293 ; 0090-3493
    ISSN (online) 1530-0293
    ISSN 0090-3493
    DOI 10.1097/CCM.0000000000005199
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Mechanisms of Basement Membrane Micro-Perforation during Cancer Cell Invasion into a 3D Collagen Gel.

    Nazari, Shayan S / Doyle, Andrew D / Yamada, Kenneth M

    Gels (Basel, Switzerland)

    2022  Volume 8, Issue 9

    Abstract: Cancer invasion through basement membranes represents the initial step of tumor dissemination and metastasis. However, little is known about how human cancer cells breach basement membranes. Here, we used a three-dimensional in vitro invasion model ... ...

    Abstract Cancer invasion through basement membranes represents the initial step of tumor dissemination and metastasis. However, little is known about how human cancer cells breach basement membranes. Here, we used a three-dimensional in vitro invasion model consisting of cancer spheroids encapsulated by a basement membrane and embedded in 3D collagen gels to visualize the early events of cancer invasion by confocal microscopy and live-cell imaging. Human breast cancer cells generated large numbers of basement membrane perforations, or holes, of varying sizes that expanded over time during cell invasion. We used a wide variety of small molecule inhibitors to probe the mechanisms of basement membrane perforation and hole expansion. Protease inhibitor treatment (BB94), led to a 63% decrease in perforation size. After myosin II inhibition (blebbistatin), the basement membrane perforation area decreased by only 15%. These treatments produced correspondingly decreased cellular breaching events. Interestingly, inhibition of actin polymerization dramatically decreased basement membrane perforation by 80% and blocked invasion. Our findings suggest that human cancer cells can primarily use proteolysis and actin polymerization to perforate the BM and to expand perforations for basement membrane breaching with a relatively small contribution from myosin II contractility.
    Language English
    Publishing date 2022-09-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2813982-3
    ISSN 2310-2861 ; 2310-2861
    ISSN (online) 2310-2861
    ISSN 2310-2861
    DOI 10.3390/gels8090567
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Cell-extracellular matrix dynamics.

    Doyle, Andrew D / Nazari, Shayan S / Yamada, Kenneth M

    Physical biology

    2022  Volume 19, Issue 2

    Abstract: The sites of interaction between a cell and its surrounding microenvironment serve as dynamic signaling hubs that regulate cellular adaptations during developmental processes, immune functions, wound healing, cell migration, cancer invasion and ... ...

    Abstract The sites of interaction between a cell and its surrounding microenvironment serve as dynamic signaling hubs that regulate cellular adaptations during developmental processes, immune functions, wound healing, cell migration, cancer invasion and metastasis, as well as in many other disease states. For most cell types, these interactions are established by integrin receptors binding directly to extracellular matrix proteins, such as the numerous collagens or fibronectin. For the cell, these points of contact provide vital cues by sampling environmental conditions, both chemical and physical. The overall regulation of this dynamic interaction involves both extracellular and intracellular components and can be highly variable. In this review, we highlight recent advances and hypotheses about the mechanisms and regulation of cell-ECM interactions, from the molecular to the tissue level, with a particular focus on cell migration. We then explore how cancer cell invasion and metastasis are deeply rooted in altered regulation of this vital interaction.
    MeSH term(s) Cell Adhesion ; Cell Communication ; Cell Movement ; Extracellular Matrix ; Integrins ; Signal Transduction
    Chemical Substances Integrins
    Language English
    Publishing date 2022-01-12
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Intramural ; Review
    ZDB-ID 2133216-2
    ISSN 1478-3975 ; 1478-3967
    ISSN (online) 1478-3975
    ISSN 1478-3967
    DOI 10.1088/1478-3975/ac4390
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Cell-3D matrix interactions: recent advances and opportunities.

    Yamada, Kenneth M / Doyle, Andrew D / Lu, Jiaoyang

    Trends in cell biology

    2022  Volume 32, Issue 10, Page(s) 883–895

    Abstract: Tissues consist of cells and their surrounding extracellular matrix (ECM). Cell-ECM interactions play crucial roles in embryonic development, differentiation, tissue remodeling, and diseases including fibrosis and cancer. Recent research advances in ... ...

    Abstract Tissues consist of cells and their surrounding extracellular matrix (ECM). Cell-ECM interactions play crucial roles in embryonic development, differentiation, tissue remodeling, and diseases including fibrosis and cancer. Recent research advances in characterizing cell-matrix interactions include detailed descriptions of hundreds of ECM and associated molecules, their complex intermolecular interactions in development and disease, identification of distinctive modes of cell migration in different 3D ECMs, and new insights into mechanisms of organ formation. Exploring the roles of the physical features of different ECM microenvironments and the bidirectional regulation of cell signaling and matrix organization emphasize the dynamic nature of these interactions, which can include feedback loops that exacerbate disease. Understanding mechanisms of cell-matrix interactions can potentially lead to targeted therapeutic interventions.
    MeSH term(s) Cell Communication ; Cell Movement ; Extracellular Matrix ; Humans ; Neoplasms ; Signal Transduction ; Tumor Microenvironment
    Language English
    Publishing date 2022-04-08
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Intramural
    ZDB-ID 30122-x
    ISSN 1879-3088 ; 0962-8924
    ISSN (online) 1879-3088
    ISSN 0962-8924
    DOI 10.1016/j.tcb.2022.03.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Antiphospholipid Syndrome with Monoclonal Gammopathy-A Mechanism for Recurrent Thrombosis?

    Doyle, Andrew J / Breen, Karen A / Hunt, Beverley J

    Thrombosis and haemostasis

    2022  Volume 121, Issue 10, Page(s) 1387–1390

    MeSH term(s) Adolescent ; Adult ; Aged ; Antiphospholipid Syndrome/complications ; Antiphospholipid Syndrome/diagnosis ; Antiphospholipid Syndrome/immunology ; Female ; Humans ; Male ; Middle Aged ; Paraproteinemias/complications ; Paraproteinemias/diagnosis ; Paraproteinemias/immunology ; Recurrence ; Retrospective Studies ; Risk Assessment ; Risk Factors ; Thrombosis/diagnosis ; Thrombosis/etiology ; Thrombosis/immunology ; Time Factors
    Language English
    Publishing date 2022-03-03
    Publishing country Germany
    Document type Letter
    ZDB-ID 518294-3
    ISSN 2567-689X ; 0340-6245
    ISSN (online) 2567-689X
    ISSN 0340-6245
    DOI 10.1055/a-1366-9379
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Generation of 3D Collagen Gels with Controlled Diverse Architectures.

    Doyle, Andrew D

    Current protocols in cell biology

    2016  Volume 72, Page(s) 10.20.1–10.20.16

    Abstract: Rat tail collagen solutions have been used as polymerizable in vitro three dimensional (3D) extracellular matrix (ECM) gels for single and collective cell migration assays as well as spheroid formation. Factors such as ECM concentration, pH, ionic ... ...

    Abstract Rat tail collagen solutions have been used as polymerizable in vitro three dimensional (3D) extracellular matrix (ECM) gels for single and collective cell migration assays as well as spheroid formation. Factors such as ECM concentration, pH, ionic concentration, and temperature can alter collagen polymerization and ECM architecture. This unit describes how to generate 3D collagen gels that have distinct architectures ranging from a highly reticular meshwork of short thin fibrils with small pores to a loose matrix consisting of stiff, parallel-bundled long fibrils by changing collagen polymerization temperature. This permits analysis of 3D cell migration in different ECM architectures found in vivo while maintaining a similar ECM concentration. Also included are collagen labeling techniques helpful for ECM visualization during live fluorescence imaging. © 2016 by John Wiley & Sons, Inc.
    MeSH term(s) Animals ; Biochemistry/methods ; Cell Culture Techniques ; Cell Survival ; Chemical Precipitation ; Collagen/chemistry ; Coloring Agents/chemistry ; Extracellular Matrix/metabolism ; Fluorescent Dyes/chemistry ; Gels/chemistry ; Imaging, Three-Dimensional ; Polymerization ; Rats ; Sodium Chloride/chemistry ; Solutions ; Succinimides/chemistry ; Tail ; Temperature
    Chemical Substances Coloring Agents ; Fluorescent Dyes ; Gels ; Solutions ; Succinimides ; Sodium Chloride (451W47IQ8X) ; Collagen (9007-34-5) ; N-hydroxysuccinimide (MJE3791M4T)
    Language English
    Publishing date 2016-09-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural
    ISSN 1934-2616
    ISSN (online) 1934-2616
    DOI 10.1002/cpcb.9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Visualization of trigeminal ganglion sensory neuronal signaling regulated by Cdk5.

    Hu, Minghan / Doyle, Andrew D / Yamada, Kenneth M / Kulkarni, Ashok B

    Cell reports

    2022  Volume 38, Issue 10, Page(s) 110458

    Abstract: The mechanisms underlying facial pain are still incompletely understood, posing major therapeutic challenges. Cyclin-dependent kinase 5 (Cdk5) is a key neuronal kinase involved in pain signaling. However, the regulatory roles of Cdk5 in facial pain ... ...

    Abstract The mechanisms underlying facial pain are still incompletely understood, posing major therapeutic challenges. Cyclin-dependent kinase 5 (Cdk5) is a key neuronal kinase involved in pain signaling. However, the regulatory roles of Cdk5 in facial pain signaling and the possibility of therapeutic intervention at the level of mouse trigeminal ganglion primary neurons remain elusive. In this study, we use optimized intravital imaging to directly compare trigeminal neuronal activities after mechanical, thermal, and chemical stimulation. We then test whether facial inflammatory pain in mice could be alleviated by the Cdk5 inhibitor peptide TFP5. We demonstrate regulation of total Ca
    MeSH term(s) Animals ; Cyclin-Dependent Kinase 5/metabolism ; Facial Pain ; Inflammation ; Mice ; Sensory Receptor Cells ; Trigeminal Ganglion
    Chemical Substances Cyclin-Dependent Kinase 5 (EC 2.7.11.1) ; Cdk5 protein, mouse (EC 2.7.11.22)
    Language English
    Publishing date 2022-03-09
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2022.110458
    Database MEDical Literature Analysis and Retrieval System OnLINE

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