LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 203

Search options

  1. Article ; Online: QHFSS DNA laboratory - ISO/IEC 17025 conformance and accreditation.

    Doyle, Sean

    Forensic science international. Synergy

    2024  Volume 8, Page(s) 100449

    Abstract: This paper reviews evidence placed before a Commission of Inquiry (CoI) established by the State of Queensland, Australia, to consider the quality and reliability of DNA evidence. It also assesses whether the criticism levied in that report, of ISO/IEC ... ...

    Abstract This paper reviews evidence placed before a Commission of Inquiry (CoI) established by the State of Queensland, Australia, to consider the quality and reliability of DNA evidence. It also assesses whether the criticism levied in that report, of ISO/IEC 17025 being insufficient to assure the quality and reliability of DNA evidence, is warranted. The main conclusion drawn is that properly applied and embraced as a means of continuous improvement, conformance with ISO/IEC 17025:2017 alone is sufficient to assure the quality and reliability of the scientific outputs from a forensic science laboratory. Furthermore, it is clear from the observations and findings of the CoI and those recorded in this paper that the forensic science laboratory in question did not conform to ISO/IEC 17025:2017. Had it done so then the risk of the quality failures that led to the CoI would at least have been reduced and perhaps even avoided.
    Language English
    Publishing date 2024-01-03
    Publishing country Netherlands
    Document type Editorial
    ISSN 2589-871X
    ISSN (online) 2589-871X
    DOI 10.1016/j.fsisyn.2023.100449
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: IRMS based evidence passes the test.

    Doyle, Sean / Chau, Thuan / Howa, John

    Science & justice : journal of the Forensic Science Society

    2023  Volume 63, Issue 6, Page(s) 743–746

    Abstract: At the two latest FIRMS (The Forensic Isotope Ratio Mass Spectrometry Network Ltd) conferences, presentations were made describing the status of IRMS based evidence in criminal proceedings [S. Doyle, T. Chau and J. Howa, IRMS based evidence passes the ' ... ...

    Abstract At the two latest FIRMS (The Forensic Isotope Ratio Mass Spectrometry Network Ltd) conferences, presentations were made describing the status of IRMS based evidence in criminal proceedings [S. Doyle, T. Chau and J. Howa, IRMS based evidence passes the 'test' (aided by FIRMS) - an important milestone, in: 8th FIRMS Conference, 2022; J. Ehleringer, J. Howa and T. Chau, Stable isotope evidence in the U.S. courts, in: 7th FIRMS Conference, 2019]. One of those cases was of particular significance in that it established the admissibility of IRMS (isotope ratio mass spectrometry) based expert testimony having satisfied the US Federal Rule of Evidence (FRE)702 'Testimony by Expert Witnesses' in the State of Colorado. While specific to the State of Colorado and the particulars of the case, the admissibility of IRMS based evidence in other cases might be supported by such a ruling. This paper provides the detail of the process by which IRMS based evidence was found to be admissible and in so doing also provides practical guidance on how to satisfy FRE 702 and meet a Daubert challenge. It also highlights the role of approval by the FIRMS Network of both practitioners and their methods in support of admissibility [The FIRMS Network Ltd., FIRMS Approved Practitioners, [Online]. Available: https://www.forensic-isotopes.org/fafp.html [Accessed 11 May 2023].].
    MeSH term(s) Humans ; Expert Testimony ; Mass Spectrometry ; Isotopes
    Chemical Substances Isotopes
    Language English
    Publishing date 2023-10-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1230910-2
    ISSN 1876-4452 ; 1355-0306
    ISSN (online) 1876-4452
    ISSN 1355-0306
    DOI 10.1016/j.scijus.2023.10.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 854: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: RadRounds and RadSim: Delivering Immersive Educational Experiences With Simulated Radiology Encounters for Medical Students.

    Doyle, Sean P / Herr, Keith D / Tuburan, Smyrna

    Journal of the American College of Radiology : JACR

    2023  Volume 20, Issue 11, Page(s) 1121–1125

    Language English
    Publishing date 2023-08-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2274861-1
    ISSN 1558-349X ; 1546-1440
    ISSN (online) 1558-349X
    ISSN 1546-1440
    DOI 10.1016/j.jacr.2023.05.019
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6636: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Effects of antifungal agents on the fungal proteome: informing on mechanisms of sensitivity and resistance.

    Owens, Rebecca A / Doyle, Sean

    Expert review of proteomics

    2021  Volume 18, Issue 3, Page(s) 185–199

    Abstract: Introduction: Antifungal agents are essential in the fight against serious fungal disease, however emerging resistance is threatening an already limited collection of therapeutics. Proteomic analyses of effects of antifungal agents can expand our ... ...

    Abstract Introduction: Antifungal agents are essential in the fight against serious fungal disease, however emerging resistance is threatening an already limited collection of therapeutics. Proteomic analyses of effects of antifungal agents can expand our understanding of multifactorial mechanisms of action and have also proven valuable to elucidate proteomic changes associated with antifungal resistance.
    Areas covered: This review covers the application of proteomic techniques to examine sensitivity and resistance to antifungals including commonly used therapeutics, amphotericin B, echinocandins and the azoles, based predominantly on studies involving
    Expert commentary: Fungal proteomics has evolved in the last decade with increased genome availability and developments in mass spectrometry. Collectively, these have led to the advancement of proteomic techniques, allowing increased coverage of the proteome. Gel-based proteomics laid the foundation for these types of studies, which has now shifted to the more powerful gel-free proteomics. This has resulted in the identification of key mediators and potential biomarkers of antifungal resistance, as well as elucidating the mechanisms of action of novel and established antifungal agents.
    MeSH term(s) Antifungal Agents/pharmacology ; Echinocandins ; Humans ; Microbial Sensitivity Tests ; Proteome ; Proteomics
    Chemical Substances Antifungal Agents ; Echinocandins ; Proteome
    Language English
    Publishing date 2021-04-12
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2299100-1
    ISSN 1744-8387 ; 1478-9450
    ISSN (online) 1744-8387
    ISSN 1478-9450
    DOI 10.1080/14789450.2021.1912601
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6686: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Target directed identification of natural bioactive compounds from filamentous fungi.

    Smith, Helen / Doyle, Sean / Murphy, Richard

    Food chemistry

    2022  Volume 405, Issue Pt A, Page(s) 134743

    Abstract: Some of the most powerful natural antimicrobial compounds originate from filamentous fungi. However, due to the diversity of compounds from plant and fungal origin, separation, isolation, and identification of bioactive constituents can be a long and ... ...

    Abstract Some of the most powerful natural antimicrobial compounds originate from filamentous fungi. However, due to the diversity of compounds from plant and fungal origin, separation, isolation, and identification of bioactive constituents can be a long and tedious process. This study explores the effectiveness of thin layer chromatography (TLC) in combination with bioautography in the separation and identification of bioactive compounds from several filamentous fungi. Ultra-performance liquid chromatography coupled to photodiode array detector (UPLC-DAD) was employed to quantitatively identify phenolic composition. The total phenolic content of the selected filamentous fungi ranged from 31.85 mg g
    Language English
    Publishing date 2022-10-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2022.134743
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 3634: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Proteomic dissection of the role of GliZ in gliotoxin biosynthesis in Aspergillus fumigatus

    Traynor, Aimee M. / Sarikaya-Bayram, Özlem / Bayram, Özgür / Antonio Calera, José / Doyle, Sean

    Fungal Genetics and Biology. 2023 May, v. 166 p.103795-

    2023  

    Abstract: Gliotoxin (GT) biosynthesis in fungi is encoded by the gli biosynthetic gene cluster. While GT addition autoinduces biosynthesis, Zn²⁺ has been shown to attenuate cluster activity, and it was speculated that identification of Zn2Cys6 binuclear ... ...

    Abstract Gliotoxin (GT) biosynthesis in fungi is encoded by the gli biosynthetic gene cluster. While GT addition autoinduces biosynthesis, Zn²⁺ has been shown to attenuate cluster activity, and it was speculated that identification of Zn2Cys6 binuclear transcription factor GliZ binding partners might provide insight into this observation. Using the Tet-ON induction system, doxycycline (DOX) presence induced GliZ fusion protein expression in, and recovery of GT biosynthesis by, A. fumigatus ΔgliZ::HA-gliZ and ΔgliZ::TAP-gliZ strains, respectively. Quantitative RT-PCR confirmed that DOX induces gli cluster gene expression (n = 5) in both A. fumigatus HA-GliZ and TAP-GliZ strains. GT biosynthesis was evident in Czapek-Dox and in Sabouraud media, however tagged GliZ protein expression was more readily detected in Sabouraud media. Unexpectedly, Zn²⁺ was essential for GliZ fusion protein expression in vivo, following 3 h DOX induction. Moreover, HA-GliZ abundance was significantly higher in either DOX/GT or DOX/Zn²⁺, compared to DOX-only. This suggests that while GT induction is still intact, Zn²⁺ inhibition of HA-GliZ production in vivo is lost. Co-immunoprecipitation revealed that GT oxidoreductase GliT associates with GliZ in the presence of GT, suggesting a potential protective role. Additional putative HA-GliZ interacting proteins included cystathionine gamma lyase, ribosomal protein L15 and serine hydroxymethyltransferase (SHMT). Total mycelial quantitative proteomic data revealed that GliT and GtmA, as well as several other gli cluster proteins, are increased in abundance or uniquely expressed with GT addition. Proteins involved in sulphur metabolism are also differentially expressed with GT or Zn²⁺ presence. Overall, we disclose that under DOX induction GliZ functionality is unexpectedly evident in zinc-replete media, subject to GT induction and that GliT appears to associate with GliZ, potentially to prevent dithiol gliotoxin (DTG)-mediated GliZ inactivation by zinc ejection.
    Keywords Aspergillus fumigatus ; biosynthesis ; cystathionine ; dissection ; doxycycline ; gene expression ; gliotoxins ; glycine hydroxymethyltransferase ; microbial genetics ; multigene family ; mycelium ; precipitin tests ; protective effect ; protein synthesis ; proteomics ; ribosomal proteins ; sulfur ; transcription factors ; zinc ; Quantitative proteomics ; Fungal pathogen ; Nutritional immunity ; Transcription factor ; LC-MS ; Mycotoxin
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier Inc.
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 1319820-8
    ISSN 1096-0937 ; 1087-1845 ; 0147-5975
    ISSN (online) 1096-0937
    ISSN 1087-1845 ; 0147-5975
    DOI 10.1016/j.fgb.2023.103795
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 79/730: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: Proteomic dissection of the role of GliZ in gliotoxin biosynthesis in Aspergillus fumigatus.

    Traynor, Aimee M / Sarikaya-Bayram, Özlem / Bayram, Özgür / Antonio Calera, José / Doyle, Sean

    Fungal genetics and biology : FG & B

    2023  Volume 166, Page(s) 103795

    Abstract: Gliotoxin (GT) biosynthesis in fungi is encoded by the gli biosynthetic gene cluster. While GT addition autoinduces biosynthesis, ... ...

    Abstract Gliotoxin (GT) biosynthesis in fungi is encoded by the gli biosynthetic gene cluster. While GT addition autoinduces biosynthesis, Zn
    MeSH term(s) Aspergillus fumigatus/genetics ; Aspergillus fumigatus/metabolism ; Gliotoxin ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Proteomics ; Zinc/metabolism
    Chemical Substances Gliotoxin (67-99-2) ; Fungal Proteins ; Zinc (J41CSQ7QDS)
    Language English
    Publishing date 2023-04-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1319820-8
    ISSN 1096-0937 ; 1087-1845 ; 0147-5975
    ISSN (online) 1096-0937
    ISSN 1087-1845 ; 0147-5975
    DOI 10.1016/j.fgb.2023.103795
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2431: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Letter to the Editor regarding: A review of recent compound-specific isotope analysis studies applied to food authentication.

    Salouros, Helen / Carter, James F / Dunn, Philip J H / Doyle, Sean P G

    Food chemistry

    2023  Volume 428, Page(s) 136776

    MeSH term(s) Food ; Carbon Isotopes/analysis ; Food Analysis
    Chemical Substances Carbon Isotopes
    Language English
    Publishing date 2023-07-01
    Publishing country England
    Document type Review ; Letter ; Comment
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2023.136776
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 3634: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Gliotoxin and related metabolites as zinc chelators: implications and exploitation to overcome antimicrobial resistance.

    Downes, Shane G / Doyle, Sean / Jones, Gary W / Owens, Rebecca A

    Essays in biochemistry

    2022  Volume 67, Issue 5, Page(s) 769–780

    Abstract: Antimicrobial resistance (AMR) is a major global problem and threat to humanity. The search for new antibiotics is directed towards targeting of novel microbial systems and enzymes, as well as augmenting the activity of pre-existing antimicrobials. ... ...

    Abstract Antimicrobial resistance (AMR) is a major global problem and threat to humanity. The search for new antibiotics is directed towards targeting of novel microbial systems and enzymes, as well as augmenting the activity of pre-existing antimicrobials. Sulphur-containing metabolites (e.g., auranofin and bacterial dithiolopyrrolones [e.g., holomycin]) and Zn2+-chelating ionophores (PBT2) have emerged as important antimicrobial classes. The sulphur-containing, non-ribosomal peptide gliotoxin, biosynthesised by Aspergillus fumigatus and other fungi exhibits potent antimicrobial activity, especially in the dithiol form (dithiol gliotoxin; DTG). Specifically, it has been revealed that deletion of the enzymes gliotoxin oxidoreductase GliT, bis-thiomethyltransferase GtmA or the transporter GliA dramatically sensitise A. fumigatus to gliotoxin presence. Indeed, the double deletion strain A. fumigatus ΔgliTΔgtmA is especially sensitive to gliotoxin-mediated growth inhibition, which can be reversed by Zn2+ presence. Moreover, DTG is a Zn2+ chelator which can eject zinc from enzymes and inhibit activity. Although multiple studies have demonstrated the potent antibacterial effect of gliotoxin, no mechanistic details are available. Interestingly, reduced holomycin can inhibit metallo-β-lactamases. Since holomycin and gliotoxin can chelate Zn2+, resulting in metalloenzyme inhibition, we propose that this metal-chelating characteristic of these metabolites requires immediate investigation to identify new antibacterial drug targets or to augment the activity of existing antimicrobials. Given that (i) gliotoxin has been shown in vitro to significantly enhance vancomycin activity against Staphylococcus aureus, and (ii) that it has been independently proposed as an ideal probe to dissect the central 'Integrator' role of Zn2+ in bacteria - we contend such studies are immediately undertaken to help address AMR.
    MeSH term(s) Gliotoxin/metabolism ; Gliotoxin/pharmacology ; Chelating Agents/pharmacology ; Fungal Proteins/metabolism ; Anti-Bacterial Agents/pharmacology ; Zinc ; Drug Resistance, Bacterial ; Sulfur
    Chemical Substances Gliotoxin (67-99-2) ; holomycin (44CF65YLF8) ; Chelating Agents ; Fungal Proteins ; Anti-Bacterial Agents ; Zinc (J41CSQ7QDS) ; dithiol (U89B11P7SC) ; Sulfur (70FD1KFU70)
    Language English
    Publishing date 2022-09-30
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1744-1358 ; 0071-1365
    ISSN (online) 1744-1358
    ISSN 0071-1365
    DOI 10.1042/EBC20220222
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article: Changing the guard.

    Doyle, Seán

    Science & justice : journal of the Forensic Science Society

    2012  Volume 52, Issue 4, Page(s) 207–208

    MeSH term(s) Forensic Sciences/legislation & jurisprudence ; Forensic Sciences/standards ; Forensic Sciences/trends ; Humans ; Laboratories/standards ; Law Enforcement ; United Kingdom
    Language English
    Publishing date 2012-12
    Publishing country England
    Document type Editorial
    ZDB-ID 1230910-2
    ISSN 1876-4452 ; 1355-0306
    ISSN (online) 1876-4452
    ISSN 1355-0306
    DOI 10.1016/j.scijus.2012.09.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 854: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top