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Article ; Online: The methods of vibrational microspectroscopy reveals long-term biochemical anomalies within the region of mechanical injury within the rat brain.

Kawon, Kamil / Setkowicz, Zuzanna / Drozdz, Agnieszka / Janeczko, Krzysztof / Chwiej, Joanna

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2021 Volume 263, Page(s) 120214

Abstract: Traumatic brain injury (TBI), meaning functional or structural brain damage which appear as a result of the application of the external physical force, constitutes the main cause of death and disability of individuals and a great socioeconomic problem. ... ...

Abstract	Traumatic brain injury (TBI), meaning functional or structural brain damage which appear as a result of the application of the external physical force, constitutes the main cause of death and disability of individuals and a great socioeconomic problem. To search for the new therapeutic strategies for TBI, better knowledge about posttraumatic pathological changes occurring in the brain is necessary. Therefore in the present paper the Fourier transform infrared microspectroscopy and Raman microscopy were used to examine local and remote biochemical changes occurring in the rat brain as a result of focal cortex injury. The site of the injury and the dorsal part of the hippocampal formation together with the above situated cortex and white matter were the subject of the study. The topographic and quantitative biochemical analysis followed with the statistical study using principal component analysis showed significant biomolecular anomalies within the lesion site but not in the area of the dorsal hippocampal formation and in the above situated white matter and cortex. The observed intralesional anomalies included significantly decreased accumulation of lipids and their structural changes within the place of injury. Also the levels of compounds containing phosphate and carbonyl groups were lower within the lesion site comparing to the surrounding cortex. The opposite relation was, in turn, found for the bands characteristic to proteins and cholesterol/cholesterol esters.
MeSH term(s)	Animals ; Brain ; Fourier Analysis ; Lipids ; Principal Component Analysis ; Rats ; Spectroscopy, Fourier Transform Infrared
Chemical Substances	Lipids
Language	English
Publishing date	2021-07-22
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2021.120214
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Article ; Online: Influence of measurement mode on the results of glioblastoma multiforme analysis with the FTIR microspectroscopy.

Wilk, Aleksandra / Drozdz, Agnieszka / Olbrich, Karolina / Janik-Olchawa, Natalia / Setkowicz, Zuzanna / Chwiej, Joanna

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2022 Volume 287, Issue Pt 2, Page(s) 122086

Abstract: Fourier Transform Infrared (FTIR) microspectroscopy is well known for its effectiveness in spectral and biochemical analyses of various materials. It enables to determine the sample biochemical composition by assigning detected frequencies, ... ...

Abstract	Fourier Transform Infrared (FTIR) microspectroscopy is well known for its effectiveness in spectral and biochemical analyses of various materials. It enables to determine the sample biochemical composition by assigning detected frequencies, characteristic for functional groups of main biological macromolecules. In analysis of tissue sections one of two measurement modes, namely transmission and transflection, is usually applied. The first one has relatively straightforward geometry, hence it is considered to be more precise and accurate. However, IR-transparent media are very fragile and expensive. Transflection does not require expensive substrates, but is more prone to disruptive influence of Mie scattering as well as electric field standing wave effect. The excessive comparison of spectra' characteristics, obtained via both measurement modes, was performed in this paper. By the means of Mann-Whitney non-parametrical U test and PCA, the comparison of results obtained with both modes and assessment of usefulness of IR spectra obtained with transmission and transflection modes to differentiate between healthy and GBM-affected tissue, were performed. The main objective of the presented research is to compare the results of FTIR analysis of unfixed biological samples performed with transflection and transmission mode. In frame of the study we demonstrated the discrepancies between results of biochemical analysis performed based on data obtained with transmission and transflection. Such observation suggests that caution should be taken in drawing conclusions from the results obtained with transflection geometry, as its more prone to disruptive effects. Despite that, IR spectra developed with both modes allowed to distinguish GBM area from healthy tissue, which proves their diagnostic potential. Especially, application of the ME-EMSC correction of spectra before PCA enhances the performance of both methods to distinguish the analysed tissue areas.
MeSH term(s)	Humans ; Fourier Analysis ; Glioblastoma/diagnosis ; Spectroscopy, Fourier Transform Infrared ; Electricity ; Spectrophotometry, Infrared
Language	English
Publishing date	2022-11-08
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2022.122086
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Article ; Online: Chemo-enzymatic Baeyer-Villiger oxidation of 4-methylcyclohexanone via kinetic resolution of racemic carboxylic acids: direct access to enantioenriched lactone.

Drożdż, Agnieszka / Chrobok, Anna

Chemical communications (Cambridge, England)

2016 Volume 52, Issue 6, Page(s) 1230–1233

Abstract: A new method for the asymmetric chemo-enzymatic Baeyer-Villiger oxidation of prochiral 4-methylcyclohexanone to (R)-4-methylcaprolactone in the presence of (±)-4-methyloctanoic acid, Candida Antarctica lipase B and 30% aq. H2O2 has been developed. A ... ...

Abstract	A new method for the asymmetric chemo-enzymatic Baeyer-Villiger oxidation of prochiral 4-methylcyclohexanone to (R)-4-methylcaprolactone in the presence of (±)-4-methyloctanoic acid, Candida Antarctica lipase B and 30% aq. H2O2 has been developed. A mechanism for the asymmetric induction based on kinetic resolution of racemic carboxylic acids is proposed.
MeSH term(s)	Carboxylic Acids/chemistry ; Cyclohexanones/chemistry ; Kinetics ; Oxidation-Reduction ; Stereoisomerism
Chemical Substances	Carboxylic Acids ; Cyclohexanones
Language	English
Publishing date	2016-01-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/c5cc08519e
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Article: Chemo-enzymatic Baeyer–Villiger oxidation of 4-methylcyclohexanone via kinetic resolution of racemic carboxylic acids: direct access to enantioenriched lactone

Drożdż, Agnieszka / Anna Chrobok

Chemical communications. 2016 Jan. 7, v. 52, no. 6

2016

Abstract: A new method for the asymmetric chemo-enzymatic Baeyer–Villiger oxidation of prochiral 4-methylcyclohexanone to (R)-4-methylcaprolactone in the presence of (±)-4-methyloctanoic acid, Candida Antarctica lipase B and 30% aq. H₂O₂ has been developed. A ... ...

Abstract	A new method for the asymmetric chemo-enzymatic Baeyer–Villiger oxidation of prochiral 4-methylcyclohexanone to (R)-4-methylcaprolactone in the presence of (±)-4-methyloctanoic acid, Candida Antarctica lipase B and 30% aq. H₂O₂ has been developed. A mechanism for the asymmetric induction based on kinetic resolution of racemic carboxylic acids is proposed.
Keywords	Pseudozyma antarctica ; carboxylic acids ; carboxylic ester hydrolases ; hydrogen peroxide ; oxidation
Language	English
Dates of publication	2016-0107
Size	p. 1230-1233.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/c5cc08519e
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The assessment of the usability of selected instrumental techniques for the elemental analysis of biomedical samples.

Planeta, Karolina / Kubala-Kukus, Aldona / Drozdz, Agnieszka / Matusiak, Katarzyna / Setkowicz, Zuzanna / Chwiej, Joanna

Scientific reports

2021 Volume 11, Issue 1, Page(s) 3704

Abstract: The fundamental role of major, minor and trace elements in different physiological and pathological processes occurring in living organism makes that elemental analysis of biomedical samples becomes more and more popular issue. The most often used tools ... ...

Abstract	The fundamental role of major, minor and trace elements in different physiological and pathological processes occurring in living organism makes that elemental analysis of biomedical samples becomes more and more popular issue. The most often used tools for analysis of the elemental composition of biological samples include Flame and Graphite Furnace Atomic Absorption Spectroscopy (F-AAS and GF-AAS), Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Each of these techniques has many advantages and limitations that should be considered in the first stage of planning the measurement procedure. Their reliability can be checked in the validation process and the precision, trueness and detection limits of elements belong to the most frequently determined validation parameters. The main purpose of this paper was the discussion of selected instrumental techniques (F-AAS, GF-AAS, ICP-OES and ICP-MS) in term of the achieved validation parameters and the usefulness in the analysis of biological samples. The focus in the detailed literature studies was also put on the methods of preparation of the biomedical samples. What is more based on the own data the usefulness of the total reflection X-ray fluorescence spectroscopy for the elemental analysis of animal tissues was examined. The detection limits of elements, precision and trueness for the technique were determined and compared with the literature data concerning other of the discussed techniques of elemental analysis. Reassuming, the following paper is to serve as a guide and comprehensive source of information concerning the validation parameters achievable in different instrumental techniques used for the elemental analysis of biomedical samples.
Language	English
Publishing date	2021-02-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-82179-3
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Article: Immunofluorescence imaging of dna damage and repair foci in human colon cancer cells

Maliszewska-Olejniczak, Kamila / Dróżdż, Agnieszka / Waluś, Martyna / Dorosz, Michał / Gryziński, Michał A

Journal of visualized experiments. 2020 June 09, , no. 160

2020

Abstract: The purpose of the manuscript is to provide a step-by-step protocol for performing immunofluorescence microscopy to study the radiation-induced DNA damage response induced by neutron-gamma mixed-beam used in boron neutron capture therapy (BNCT). ... ...

Abstract	The purpose of the manuscript is to provide a step-by-step protocol for performing immunofluorescence microscopy to study the radiation-induced DNA damage response induced by neutron-gamma mixed-beam used in boron neutron capture therapy (BNCT). Specifically, the proposed methodology is applied for the detection of repair proteins activation which can be visualized as foci using antibodies specific to DNA double-strand breaks (DNA-DSBs). DNA repair foci were assessed by immunofluorescence in colon cancer cells (HCT-116) after irradiation with the neutron-mixed beam. DNA-DSBs are the most genotoxic lesions and are repaired in mammalian cells by two major pathways: non-homologous end-joining pathway (NHEJ) and homologous recombination repair (HRR). The frequencies of foci, immunochemically stained, for commonly used markers in radiobiology like γ-H2AX, 53BP1 are associated with DNA-DSB number and are considered as efficient and sensitive markers for monitoring the induction and repair of DNA-DSBs. It was established that γ-H2AX foci attract repair proteins, leading to a higher concentration of repair factors near a DSB. To monitor DNA damage at the cellular level, immunofluorescence analysis for the presence of DNA-PKcs representative repair protein foci from the NHEJ pathway and Rad52 from the HRR pathway was planned. We have developed and introduced a reliable immunofluorescence staining protocol for the detection of radiation-induced DNA damage response with antibodies specific for repair factors from NHEJ and HRR pathways and observed radiation-induced foci (RIF). The proposed methodology can be used for investigating repair protein that is highly activated in the case of neutron-mixed beam radiation, thereby indicating the dominance of the repair pathway.
Keywords	DNA damage ; DNA repair ; antibodies ; boron ; colorectal neoplasms ; fluorescence microscopy ; fluorescent antibody technique ; human diseases ; image analysis ; irradiation ; mammals ; monitoring ; mutagens ; neoplasm cells ; neutrons ; proteins ; staining ; therapeutics
Language	English
Dates of publication	2020-0609
Size	p. e61399.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/61399
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Article: Organ Metallome Processed with Chemometric Methods Enable the Determination of Elements that May Serve as Markers of Exposure to Iron Oxide Nanoparticles in Male Rats

Rugiel, Marzena / Drozdz, Agnieszka / Matusiak, Katarzyna / Setkowicz, Zuzanna / Klodowski, Krzysztof / Chwiej, Joanna

Biological trace element research. 2020 Dec., v. 198, no. 2

2020

Abstract: The systemic influence of iron oxide nanoparticles on the elemental homeostasis of key organs was examined in male rats. In tissues taken at different intervals from nanoparticles injection, the dynamics of elemental changes was analyzed. The organ ... ...

Abstract	The systemic influence of iron oxide nanoparticles on the elemental homeostasis of key organs was examined in male rats. In tissues taken at different intervals from nanoparticles injection, the dynamics of elemental changes was analyzed. The organ metallome was studied using total reflection X-ray fluorescence. The obtained data were processed with advanced cluster and discriminant analyses—to classify the tissues according to their organs of origin and to distinguish accurately the nanoparticle-treated and normal rats. Additionally, in the case of liver and heart, it was possible to determine the elements of highest significance for different treatments, which may serve as markers of exposure to iron oxide nanoparticles.
Keywords	X-radiation ; chemometrics ; fluorescence ; heart ; homeostasis ; iron oxides ; liver ; males ; metallome ; research ; trace elements
Language	English
Dates of publication	2020-12
Size	p. 602-616.
Publishing place	Springer US
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	445336-0
ISSN	1559-0720 ; 0163-4984
ISSN (online)	1559-0720
ISSN	0163-4984
DOI	10.1007/s12011-020-02104-z
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Article ; Online: Biochemical changes of macrophages and U87MG cells occurring as a result of the exposure to iron oxide nanoparticles detected with the Raman microspectroscopy.

Janik-Olchawa, Natalia / Drozdz, Agnieszka / Wajda, Aleksandra / Sitarz, Maciej / Planeta, Karolina / Setkowicz, Zuzanna / Ryszawy, Damian / Kmita, Angelika / Chwiej, Joanna

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2022 Volume 278, Page(s) 121337

Abstract: The core size of iron oxide nanoparticles (IONPs) is a crucial factor defining not only their magnetic properties but also toxicological profile and biocompatibility. On the other hand, particular IONPs may induce different biological response depending ... ...

Abstract	The core size of iron oxide nanoparticles (IONPs) is a crucial factor defining not only their magnetic properties but also toxicological profile and biocompatibility. On the other hand, particular IONPs may induce different biological response depending on the dose, exposure time, but mainly depending on the examined system. New light on this problem may be shed by the information concerning biomolecular anomalies appearing in various cell lines in response to the action of IONPs with different core diameters and this was accomplished in the present study. Using Raman microscopy we studied the abnormalities in the accumulation of proteins, lipids and organic matter within the nucleus, cytoplasm and cellular membrane of macrophages, HEK293T and U87MG cell line occurring as a result of 24-hour long exposure to PEG-coated magnetite IONPs. The examined nanoparticles had 5, 10 and 30 nm cores and were administered in doses 5 and 25 μg Fe/ml. The obtained results showed significant anomalies in biochemical composition of macrophages and the U87MG cells, but not the HEK293T cells, occurring as a result of exposure to all of the examined nanoparticles. However, IONPs with 10 nm core diminished the accumulation of biomolecules in cells only when they were administered at a larger dose. The Raman spectra recorded for the macrophages subjected to 30 nm IONPs and for the U87MG cells exposed to 5 and 10 nm showed the presence of additional bands in the wavenumber range 1700-2400 cm
MeSH term(s)	Ferric Compounds/toxicity ; Ferrosoferric Oxide ; HEK293 Cells ; Humans ; Macrophages ; Magnetite Nanoparticles/chemistry ; Magnetite Nanoparticles/toxicity ; Nanoparticles/chemistry
Chemical Substances	Ferric Compounds ; Magnetite Nanoparticles ; Ferrosoferric Oxide (XM0M87F357)
Language	English
Publishing date	2022-05-02
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2022.121337
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells.

Maliszewska-Olejniczak, Kamila / Dróżdż, Agnieszka / Waluś, Martyna / Dorosz, Michał / Gryziński, Michał A

Journal of visualized experiments : JoVE

2020 , Issue 160

Abstract	The purpose of the manuscript is to provide a step-by-step protocol for performing immunofluorescence microscopy to study the radiation-induced DNA damage response induced by neutron-gamma mixed-beam used in boron neutron capture therapy (BNCT). Specifically, the proposed methodology is applied for the detection of repair proteins activation which can be visualized as foci using antibodies specific to DNA double-strand breaks (DNA-DSBs). DNA repair foci were assessed by immunofluorescence in colon cancer cells (HCT-116) after irradiation with the neutron-mixed beam. DNA-DSBs are the most genotoxic lesions and are repaired in mammalian cells by two major pathways: non-homologous end-joining pathway (NHEJ) and homologous recombination repair (HRR). The frequencies of foci, immunochemically stained, for commonly used markers in radiobiology like γ-H2AX, 53BP1 are associated with DNA-DSB number and are considered as efficient and sensitive markers for monitoring the induction and repair of DNA-DSBs. It was established that γ-H2AX foci attract repair proteins, leading to a higher concentration of repair factors near a DSB. To monitor DNA damage at the cellular level, immunofluorescence analysis for the presence of DNA-PKcs representative repair protein foci from the NHEJ pathway and Rad52 from the HRR pathway was planned. We have developed and introduced a reliable immunofluorescence staining protocol for the detection of radiation-induced DNA damage response with antibodies specific for repair factors from NHEJ and HRR pathways and observed radiation-induced foci (RIF). The proposed methodology can be used for investigating repair protein that is highly activated in the case of neutron-mixed beam radiation, thereby indicating the dominance of the repair pathway.
MeSH term(s)	Colonic Neoplasms/diagnostic imaging ; Colonic Neoplasms/genetics ; DNA Damage/immunology ; DNA Repair/immunology ; Fluorescent Antibody Technique/methods ; Humans
Language	English
Publishing date	2020-06-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/61399
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Organ Metallome Processed with Chemometric Methods Enable the Determination of Elements that May Serve as Markers of Exposure to Iron Oxide Nanoparticles in Male Rats.

Rugiel, Marzena / Drozdz, Agnieszka / Matusiak, Katarzyna / Setkowicz, Zuzanna / Klodowski, Krzysztof / Chwiej, Joanna

Biological trace element research

2020 Volume 198, Issue 2, Page(s) 602–616

Abstract	The systemic influence of iron oxide nanoparticles on the elemental homeostasis of key organs was examined in male rats. In tissues taken at different intervals from nanoparticles injection, the dynamics of elemental changes was analyzed. The organ metallome was studied using total reflection X-ray fluorescence. The obtained data were processed with advanced cluster and discriminant analyses-to classify the tissues according to their organs of origin and to distinguish accurately the nanoparticle-treated and normal rats. Additionally, in the case of liver and heart, it was possible to determine the elements of highest significance for different treatments, which may serve as markers of exposure to iron oxide nanoparticles.
MeSH term(s)	Animals ; Biomarkers ; Liver ; Magnetic Iron Oxide Nanoparticles ; Male ; Nanoparticles ; Rats
Chemical Substances	Biomarkers
Language	English
Publishing date	2020-03-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	445336-0
ISSN	1559-0720 ; 0163-4984
ISSN (online)	1559-0720
ISSN	0163-4984
DOI	10.1007/s12011-020-02104-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

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