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  1. Article ; Online: Extracellular matrix assembly stress initiates Drosophila central nervous system morphogenesis.

    Serna-Morales, Eduardo / Sánchez-Sánchez, Besaiz J / Marcotti, Stefania / Nichols, Angus / Bhargava, Anushka / Dragu, Anca / Hirvonen, Liisa M / Díaz-de-la-Loza, María-Del-Carmen / Mink, Matyas / Cox, Susan / Rayfield, Emily / Lee, Rachel M / Hobson, Chad M / Chew, Teng-Leong / Stramer, Brian M

    Developmental cell

    2023  Volume 58, Issue 10, Page(s) 825–835.e6

    Abstract: Forces controlling tissue morphogenesis are attributed to cellular-driven activities, and any role for extracellular matrix (ECM) is assumed to be passive. However, all polymer networks, including ECM, can develop autonomous stresses during their ... ...

    Abstract Forces controlling tissue morphogenesis are attributed to cellular-driven activities, and any role for extracellular matrix (ECM) is assumed to be passive. However, all polymer networks, including ECM, can develop autonomous stresses during their assembly. Here, we examine the morphogenetic function of an ECM before reaching homeostatic equilibrium by analyzing de novo ECM assembly during Drosophila ventral nerve cord (VNC) condensation. Asymmetric VNC shortening and a rapid decrease in surface area correlate with the exponential assembly of collagen IV (Col4) surrounding the tissue. Concomitantly, a transient developmentally induced Col4 gradient leads to coherent long-range flow of ECM, which equilibrates the Col4 network. Finite element analysis and perturbation of Col4 network formation through the generation of dominant Col4 mutations that affect assembly reveal that VNC morphodynamics is partially driven by a sudden increase in ECM-driven surface tension. These data suggest that ECM assembly stress and associated network instabilities can actively participate in tissue morphogenesis.
    MeSH term(s) Animals ; Drosophila/genetics ; Extracellular Matrix/physiology ; Morphogenesis/physiology ; Central Nervous System
    Language English
    Publishing date 2023-04-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2054967-2
    ISSN 1878-1551 ; 1534-5807
    ISSN (online) 1878-1551
    ISSN 1534-5807
    DOI 10.1016/j.devcel.2023.03.019
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  2. Article: Low genetic variability of Rhinolophus mehelyi (Mehely's horseshoe bat) in Romania

    Dragu, Anca / Ivailo Borissov

    Acta theriologica. 2011 Oct., v. 56, no. 4

    2011  

    Abstract: Rhinolophus mehelyi (Mehely's horseshoe bat) is a vulnerable species with an increasingly fragmented distribution. In Romania, populations of R. mehelyi have experienced a dramatic decline over the past 50 years, and the current population size is ... ...

    Abstract Rhinolophus mehelyi (Mehely's horseshoe bat) is a vulnerable species with an increasingly fragmented distribution. In Romania, populations of R. mehelyi have experienced a dramatic decline over the past 50 years, and the current population size is estimated at only 100 adult individuals inhabiting almost exclusively the Limanu cave. In the present study, we investigated the genetic consequences of population decline for the viability of the remaining population of R. mehelyi in Romania. We sequenced and analyzed a 359-bp fragment of the mitochondrial control region from the only known Romanian population and compared it with two geographically close colonies from Bulgaria. A single haplotype was found in the Romanian population compared to 10 in the Bulgarian population, suggesting genetic isolation.
    Keywords Rhinolophus ; adults ; genetic variation ; haplotypes ; mitochondria ; population dynamics ; population size ; viability ; vulnerable species ; Bulgaria ; Romania
    Language English
    Dates of publication 2011-10
    Size p. 383.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ISSN 0001-7051
    DOI 10.1007/s13364-011-0043-z
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Protocol for intervention-free quantification of protein turnover rate by steady-state modeling.

    Marcotti, Stefania / Sánchez-Sánchez, Besaiz Jose / Serna-Morales, Eduardo / Dragu, Anca / Díaz-de-la-Loza, María-Del-Carmen / Matsubayashi, Yutaka / Stramer, Brian Marc

    STAR protocols

    2021  Volume 2, Issue 1, Page(s) 100377

    Abstract: Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeostasis. This ... ...

    Abstract Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeostasis. This approach can be used to calculate production and degradation rates and to infer protein half-life. This model was successfully employed to quantify turnover during
    MeSH term(s) Animals ; Computational Biology/methods ; Drosophila/metabolism ; Gene Expression/genetics ; Homeostasis ; Kinetics ; Models, Theoretical ; Proteins/metabolism ; Proteolysis ; RNA, Messenger/metabolism
    Chemical Substances Proteins ; RNA, Messenger
    Language English
    Publishing date 2021-03-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2021.100377
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis.

    Matsubayashi, Yutaka / Sánchez-Sánchez, Besaiz Jose / Marcotti, Stefania / Serna-Morales, Eduardo / Dragu, Anca / Díaz-de-la-Loza, María-Del-Carmen / Vizcay-Barrena, Gema / Fleck, Roland Alexander / Stramer, Brian Marc

    Developmental cell

    2020  Volume 54, Issue 1, Page(s) 33–42.e9

    Abstract: The extracellular matrix (ECM) is a polymer network hypothesized to form a stable cellular scaffold. While the ECM can undergo acute remodeling during embryogenesis, it is experimentally difficult to determine whether basal turnover is also important. ... ...

    Abstract The extracellular matrix (ECM) is a polymer network hypothesized to form a stable cellular scaffold. While the ECM can undergo acute remodeling during embryogenesis, it is experimentally difficult to determine whether basal turnover is also important. Most studies of homeostatic turnover assume an initial steady-state balance of production and degradation and measure half-life by quantifying the rate of decay after experimental intervention (e.g., pulse labeling). Here, we present an intervention-free approach to mathematically model basal ECM turnover during embryogenesis by exploiting our ability to live image de novo ECM development in Drosophila to quantify production from initiation to homeostasis. This reveals rapid turnover (half-life ∼7-10 h), which we confirmed by in vivo pulse-chase experiments. Moreover, ECM turnover is partially dependent on proteolysis and network interactions, and slowing turnover affects tissue morphogenesis. These data demonstrate that embryonic ECM undergoes constant replacement, which is likely necessary to maintain network plasticity to accommodate growth and morphogenesis.
    MeSH term(s) Animals ; Basement Membrane/metabolism ; Drosophila Proteins/metabolism ; Drosophila melanogaster ; Epithelial Cells/cytology ; Epithelial Cells/metabolism ; Extracellular Matrix/metabolism ; Extracellular Matrix Proteins/metabolism ; Homeostasis ; Models, Theoretical ; Morphogenesis
    Chemical Substances Drosophila Proteins ; Extracellular Matrix Proteins
    Language English
    Publishing date 2020-06-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2054967-2
    ISSN 1878-1551 ; 1534-5807
    ISSN (online) 1878-1551
    ISSN 1534-5807
    DOI 10.1016/j.devcel.2020.06.005
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  5. Article ; Online: First record of Pipistrellus kuhlii Kuhl, 1817 (Chiroptera

    Dragu Anca / Munteanu Ioana / Olteanu V.

    Archives of Biological Sciences, Vol 59, Iss 3, Pp 243-

    vespertilionidae) from Dobrogea (Romania)

    2007  Volume 247

    Abstract: Pipistrellus kuhlii is recorded in the Dobrogea District for the first time. On the 16th of July 2006, a nursery colony was discovered on the second floor of a building in Constanţa (2.5 m a. s. l., 44°10.4’N 28°38.3’E). External characters as well as ... ...

    Abstract Pipistrellus kuhlii is recorded in the Dobrogea District for the first time. On the 16th of July 2006, a nursery colony was discovered on the second floor of a building in Constanţa (2.5 m a. s. l., 44°10.4’N 28°38.3’E). External characters as well as cranial and dental measurements of two specimens are given. .
    Keywords Chiroptera ; Pipistrellus kuhlii ; nursery colony ; Dobrogea ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2007-01-01T00:00:00Z
    Publisher University of Belgrade, University of Novi Sad
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response.

    Sánchez-Sánchez, Besaiz J / Urbano, José M / Comber, Kate / Dragu, Anca / Wood, Will / Stramer, Brian / Martín-Bermudo, María D

    Cell reports

    2017  Volume 21, Issue 6, Page(s) 1461–1470

    Abstract: The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous ... ...

    Abstract The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous matrix presented to the moving cells. In contrast to this view, we show here that Drosophila embryonic hemocytes deposit their own laminins in streak-like structures to migrate efficiently throughout the embryo. With the help of transplantation experiments, live microscopy, and image quantification, we demonstrate that autocrine-produced laminin regulates hemocyte migration by controlling lamellipodia dynamics, stability, and persistence. Proper laminin deposition is regulated by the RabGTPase Rab8, which is highly expressed and required in hemocytes for lamellipodia dynamics and migration. Our results thus support a model in which, during embryogenesis, the Rab8-regulated autocrine deposition of laminin reinforces directional and effective migration by stabilizing cellular protrusions and strengthening otherwise transient adhesion states.
    MeSH term(s) Animals ; Cell Movement ; Cells, Cultured ; Drosophila/growth & development ; Drosophila/metabolism ; Drosophila Proteins/metabolism ; Embryo, Nonmammalian/cytology ; Embryonic Development ; Extracellular Matrix/metabolism ; GTP Phosphohydrolases/metabolism ; Hemocytes/cytology ; Hemocytes/metabolism ; Laminin/metabolism ; Microscopy, Fluorescence ; Pseudopodia/physiology
    Chemical Substances Drosophila Proteins ; Laminin ; GTP Phosphohydrolases (EC 3.6.1.-) ; Rab8 protein, Drosophila (EC 3.6.1.-)
    Language English
    Publishing date 2017-11-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2017.10.047
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: In vivo

    Aflorei, Elena Daniela / Klapholz, Benjamin / Chen, Chenghao / Radian, Serban / Dragu, Anca Neluta / Moderau, Nina / Prodromou, Chrisostomos / Ribeiro, Paulo S / Stanewsky, Ralf / Korbonits, Márta

    Journal of medical genetics

    2018  Volume 55, Issue 8, Page(s) 522–529

    Abstract: Background: Heterozygous germline loss-of-function mutations in the aryl hydrocarbon receptor-interacting protein gene (: Objective: To develop an : Methods: We generated a null mutant of the : Results: We found that human : Conclusion: ... ...

    Abstract Background: Heterozygous germline loss-of-function mutations in the aryl hydrocarbon receptor-interacting protein gene (
    Objective: To develop an
    Methods: We generated a null mutant of the
    Results: We found that human
    Conclusion: Our
    MeSH term(s) Alleles ; Amino Acid Sequence ; Amino Acid Substitution ; Animals ; Animals, Genetically Modified ; Biological Assay ; Drosophila melanogaster ; Female ; Gene Expression ; Genetic Association Studies/methods ; Genetic Predisposition to Disease ; Genetic Testing ; Genotype ; Humans ; Intracellular Signaling Peptides and Proteins/chemistry ; Intracellular Signaling Peptides and Proteins/genetics ; Loss of Function Mutation ; Loss of Heterozygosity ; Male ; Models, Molecular ; Mutation, Missense ; Pituitary Neoplasms/diagnosis ; Pituitary Neoplasms/genetics ; Protein Conformation ; Structure-Activity Relationship
    Chemical Substances Intracellular Signaling Peptides and Proteins ; aryl hydrocarbon receptor-interacting protein
    Language English
    Publishing date 2018-04-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 220881-7
    ISSN 1468-6244 ; 0022-2593
    ISSN (online) 1468-6244
    ISSN 0022-2593
    DOI 10.1136/jmedgenet-2017-105191
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  8. Article ; Online: A Moving Source of Matrix Components Is Essential for De Novo Basement Membrane Formation.

    Matsubayashi, Yutaka / Louani, Adam / Dragu, Anca / Sánchez-Sánchez, Besaiz J / Serna-Morales, Eduardo / Yolland, Lawrence / Gyoergy, Attila / Vizcay, Gema / Fleck, Roland A / Heddleston, John M / Chew, Teng-Leong / Siekhaus, Daria E / Stramer, Brian M

    Current biology : CB

    2017  Volume 27, Issue 22, Page(s) 3526–3534.e4

    Abstract: The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is ...

    Abstract The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is unknown how all of these components are generated and subsequently constructed to form a fully mature BM in the living animal. Although BM formation is thought to simply involve a process of self-assembly [2], this concept suffers from a number of logistical issues when considering its construction in vivo. First, incorporation of BM components appears to be hierarchical [3-5], yet it is unclear whether their production during embryogenesis must also be regulated in a temporal fashion. Second, many BM proteins are produced not only by the cells residing on the BM but also by surrounding cell types [6-9], and it is unclear how large, possibly insoluble protein complexes [10] are delivered into the matrix. Here we exploit our ability to live image and genetically dissect de novo BM formation during Drosophila development. This reveals that there is a temporal hierarchy of BM protein production that is essential for proper component incorporation. Furthermore, we show that BM components require secretion by migrating macrophages (hemocytes) during their developmental dispersal, which is critical for embryogenesis. Indeed, hemocyte migration is essential to deliver a subset of ECM components evenly throughout the embryo. This reveals that de novo BM construction requires a combination of both production and distribution logistics allowing for the timely delivery of core components.
    MeSH term(s) Animals ; Basement Membrane/metabolism ; Basement Membrane/physiology ; Cell Movement/physiology ; Collagen/metabolism ; Drosophila Proteins/metabolism ; Drosophila melanogaster/embryology ; Drosophila melanogaster/metabolism ; Epithelial Cells/metabolism ; Extracellular Matrix/metabolism ; Extracellular Matrix/physiology ; Macrophages/metabolism
    Chemical Substances Drosophila Proteins ; Collagen (9007-34-5)
    Language English
    Publishing date 2017-11-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1071731-6
    ISSN 1879-0445 ; 0960-9822
    ISSN (online) 1879-0445
    ISSN 0960-9822
    DOI 10.1016/j.cub.2017.10.001
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