Article: [Effects and mechanisms of Xiao-Ai-Ping injection on angiogenesis].
Yao xue xue bao = Acta pharmaceutica Sinica
2016 Volume 51, Issue 2, Page(s) 309–315
Abstract: This study was designed to investigate the effect of Xiao-Ai-Ping injection on cancer angiogenesis. CCK8 assay and Brd U incorporation immunofluorescence assay were used to detect the effect of Xiao-Ai-Ping injection on HUVECs proliferation; wound ... ...
| Abstract | This study was designed to investigate the effect of Xiao-Ai-Ping injection on cancer angiogenesis. CCK8 assay and Brd U incorporation immunofluorescence assay were used to detect the effect of Xiao-Ai-Ping injection on HUVECs proliferation; wound healing assay and transwell assay were employed to test the effect of Xiao-Ai-Ping injection on HUVECs migration. The anti-angiogenic effect of Xiao-Ai-Ping injection was examined by tube formation assay, rat aortic ring assay and chicken chorioallantoic membrane(CAM) assay. ELISA assay was used to measure the secretion of vascular endothelial growth factor(VEGF); and the activation of vascular endothelial growth factor receptor 2(VEGFR2) protein and its downstream signaling pathways were examined by Western blot. Our data demonstrated that Xiao-Ai-Ping injection inhibited HUVECs proliferation in a time- and dose-dependent manner, and the IC(50) (mg·m L(-1)) values for 24, 48 and 72 h were 48.7 ± 7.14, 29.1 ±2.25 and 22.0 ± 4.53, individually. Xiao-Ai-Ping injection inhibited HUVECs DNA synthesis and migration. Xiao-Ai-Ping injection suppressed HUVECs tube formation, and reduced microvessel sprouting from rat aortic rings and vessel growth in CAMs. Furthermore, Xiao-Ai-Ping injection attenuated the secretion of VEGF, and inhibited the expression of p-VEGFR2 and phosphorylation of protein kinase B(p-AKT). We conclude that Xiao-Ai-Ping injection inhibits angiogenesis by down-regulation of VEGF signaling and AKT pathway. |
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| MeSH term(s) | Angiogenesis Inhibitors/pharmacology ; Animals ; Cell Movement ; Chickens ; Chorioallantoic Membrane ; Drugs, Chinese Herbal/pharmacology ; Human Umbilical Vein Endothelial Cells/drug effects ; Humans ; Neovascularization, Pathologic/drug therapy ; Phosphorylation ; Proto-Oncogene Proteins c-akt/metabolism ; Rats ; Signal Transduction ; Vascular Endothelial Growth Factor A/metabolism ; Vascular Endothelial Growth Factor Receptor-2/metabolism ; Wound Healing | |||||
| Chemical Substances | Angiogenesis Inhibitors ; Drugs, Chinese Herbal ; Marsdeniae tenacissimae extract ; Vascular Endothelial Growth Factor A ; KDR protein, human (EC 2.7.10.1) ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) | |||||
| Language | Chinese | |||||
| Publishing date | 2016 | |||||
| Publishing country | China | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 788758-9 | |||||
| ISSN | 0513-4870 | |||||
| ISSN | 0513-4870 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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