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Article ; Online: Sulfur migration mechanism of pig manure in supercritical water: A combined experimental and DFT study.

Zhang, Shuyuan / Du, Mingming / Ge, Hui / Rong, Siqi / Chen, Yunan / Guo, Liejin

2024 Volume 471, Page(s) 134376

Abstract: Pig manure (PM) is a high concentration organic waste rich in sulfur, and its biofuel contains various sulfur-containing pollutants, which reduces the safety of the products. Supercritical water (SCW) can dissolve most organic matter, which is a ... ...

Abstract	Pig manure (PM) is a high concentration organic waste rich in sulfur, and its biofuel contains various sulfur-containing pollutants, which reduces the safety of the products. Supercritical water (SCW) can dissolve most organic matter, which is a technology worthy of further study. In order to reduce sulfur pollution in the process of PM resource utilization and better control the conversion path of sulfur, it is necessary to explore the migration mechanism of sulfur in the whole PM-SCW gasification process. The experimental results indicated that H
Language	English
Publishing date	2024-04-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2024.134376
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Article ; Online: Saponin extract from Achyranthes bidentata Blume alleviates disuse-induced muscle atrophy through PI3K/Akt signaling pathway

Shi, Yi / Zhang, Zhuang-Wei / Du, Ming-Ming / Wu, Jing / Li, Jian-Xin

Journal of Ethnopharmacology. 2023 Aug., v. 312 p.116458-

2023

Abstract: The roots of Achyranthes bidentata Blume are one of the regularly used herbal drugs in Chinese medicine, and has been applied for strengthening the muscle and bone for a long time. However, its effect on muscle remains unclear. This paper aims to explore ...

Abstract	The roots of Achyranthes bidentata Blume are one of the regularly used herbal drugs in Chinese medicine, and has been applied for strengthening the muscle and bone for a long time. However, its effect on muscle remains unclear. This paper aims to explore the anti-muscle atrophy effect of A. bidentata, and to clarify the possible signaling pathways involved. The saponin extract of the roots of A. bidentata (ABSE) was prepared and analyzed, and its activity on myoblast differentiation was assayed with C2C12 cell culture. ABSE was then orally administered at dosage of 35, 70 and 140 mg/kg/day to disuse-induced muscle atrophy mice. The studies on mice body weight and muscle quality were conducted, and Western blot was used for exploring the possible signaling pathways involved in the muscle protective action aided with transcriptome analysis. The total saponin content of ABSE was 59.1%. ABSE promoted the C2C12 cells differentiation to myotube in C2C12 differentiation assay. Further study with disuse-induced muscle atrophy mice model demonstrated that ABSE significantly increased muscle fiber diameter as well as the proportion of slow muscle fibers. Possible mechanism study aided with transcriptome analysis revealed that ABSE alleviated muscle atrophy at least through activation of PI3K/Akt pathway in vivo & vitro. The saponin extract of the root of A. bidentata (ABSE) has a protective effect on muscle atrophy, and showed a considerable potential in prevention and treatment of muscle atrophy.
Keywords	Achyranthes japonica ; Oriental traditional medicine ; Western blotting ; body weight ; cell culture ; models ; muscle fibers ; muscles ; muscular atrophy ; myoblasts ; myotubes ; protective effect ; saponins ; transcriptomics ; Saponin extract ; Achyranthes bidentata ; Muscle atrophy ; Transcriptome analysis ; PI3K/Akt
Language	English
Dates of publication	2023-08
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116458
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: HA-20 prevents hepatocyte steatosis in metabolic-associated fatty liver disease via regulating Ca

Wang, Jie / Du, Ming-Ming / Du, Yun / Li, Jian-Xin

European journal of pharmacology

2022 Volume 921, Page(s) 174838

Abstract: Metabolic-associated fatty liver disease (MAFLD) is caused by hepatocyte steatosis and is associated with obesity, type II diabetes, and heart disease. There are currently no effective drugs to treat MAFLD. This study explored the effect of HA-20, an ... ...

Abstract	Metabolic-associated fatty liver disease (MAFLD) is caused by hepatocyte steatosis and is associated with obesity, type II diabetes, and heart disease. There are currently no effective drugs to treat MAFLD. This study explored the effect of HA-20, an oleanolic acid derivative, on hepatocyte steatosis in MAFLD. HepG2, L02, and AML12 cells were developed using oleic acid for in vitro MAFLD cell assays, and a high-fat diet + high-fructose diet-induced (HFHF) MAFLD mouse model was established for in vivo studies. The results demonstrated that HA-20 prevented hepatocyte steatosis in cell assays and caused 26.3, 57.7 and 70.0% inhibition of triglyceride (TG) levels in the 5.0, 10.0 and 20.0 μM HA-20 groups, respectively. The EC
MeSH term(s)	Animals ; Diabetes Mellitus, Type 2/metabolism ; Diet, High-Fat/adverse effects ; Fatty Liver/chemically induced ; Hepatocytes/metabolism ; Lipogenesis ; Liver/metabolism ; Mice ; Non-alcoholic Fatty Liver Disease/metabolism
Language	English
Publishing date	2022-02-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80121-5
ISSN	1879-0712 ; 0014-2999
ISSN (online)	1879-0712
ISSN	0014-2999
DOI	10.1016/j.ejphar.2022.174838
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Zs.A 522: Show issues

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Article ; Online: Directional transformation and migration pathways of nitrogen during pig manure supercritical water gasification.

Du, Mingming / Xie, Anlan / Wei, Wenwen / Liu, Shi / Chen, Yunan / Guo, Liejin

Bioresource technology

2023 Volume 394, Page(s) 130256

Abstract: Nitrogen is a valuable nutrient element in pig manure. This work focuses on investigating the distribution, directional transformation, and migration pathways to facilitate the recovery of nitrogen from supercritical water gasification products. Results ... ...

Abstract	Nitrogen is a valuable nutrient element in pig manure. This work focuses on investigating the distribution, directional transformation, and migration pathways to facilitate the recovery of nitrogen from supercritical water gasification products. Results indicated that no nitrogen-containing gas was detected and 12.65 % of nitrogen remained in solid products. 82.49 % of nitrogen migrated into liquid products, which are predominated by ammonia. Catalysts were employed to promote the conversion of solid nitrogen to liquid nitrogen and organic nitrogen to ammonia. Finally, 85 % of nitrogen is enriched into liquid products and ammonia predominated the liquid nitrogen. The percentage of ammonia increased to 97.51 % at 620 °C in the presence of potassium carbonate. The migration pathways indicated that nitrogen was transformed into ammonia by various intermediates such as indole. The rest of the nitrogen remained in solid products with stable quaternary-nitrogen. These findings provide valuable insights into nitrogen management and recovery.
MeSH term(s)	Animals ; Swine ; Nitrogen/analysis ; Manure ; Ammonia ; Water ; Gases
Chemical Substances	Nitrogen (N762921K75) ; Manure ; Ammonia (7664-41-7) ; Water (059QF0KO0R) ; Gases
Language	English
Publishing date	2023-12-23
Publishing country	England
Document type	Journal Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2023.130256
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Article ; Online: Saponin extract from Achyranthes bidentata Blume alleviates disuse-induced muscle atrophy through PI3K/Akt signaling pathway.

Shi, Yi / Zhang, Zhuang-Wei / Du, Ming-Ming / Wu, Jing / Li, Jian-Xin

Journal of ethnopharmacology

2023 Volume 312, Page(s) 116458

Abstract: Ethnopharmacological relevance: The roots of Achyranthes bidentata Blume are one of the regularly used herbal drugs in Chinese medicine, and has been applied for strengthening the muscle and bone for a long time. However, its effect on muscle remains ... ...

Abstract	Ethnopharmacological relevance: The roots of Achyranthes bidentata Blume are one of the regularly used herbal drugs in Chinese medicine, and has been applied for strengthening the muscle and bone for a long time. However, its effect on muscle remains unclear. Aim of the study: This paper aims to explore the anti-muscle atrophy effect of A. bidentata, and to clarify the possible signaling pathways involved. Materials and methods: The saponin extract of the roots of A. bidentata (ABSE) was prepared and analyzed, and its activity on myoblast differentiation was assayed with C2C12 cell culture. ABSE was then orally administered at dosage of 35, 70 and 140 mg/kg/day to disuse-induced muscle atrophy mice. The studies on mice body weight and muscle quality were conducted, and Western blot was used for exploring the possible signaling pathways involved in the muscle protective action aided with transcriptome analysis. Results: The total saponin content of ABSE was 59.1%. ABSE promoted the C2C12 cells differentiation to myotube in C2C12 differentiation assay. Further study with disuse-induced muscle atrophy mice model demonstrated that ABSE significantly increased muscle fiber diameter as well as the proportion of slow muscle fibers. Possible mechanism study aided with transcriptome analysis revealed that ABSE alleviated muscle atrophy at least through activation of PI3K/Akt pathway in vivo & vitro. Conclusions: The saponin extract of the root of A. bidentata (ABSE) has a protective effect on muscle atrophy, and showed a considerable potential in prevention and treatment of muscle atrophy.
MeSH term(s)	Mice ; Animals ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Proto-Oncogene Proteins c-akt ; Achyranthes ; Phosphatidylinositol 3-Kinases ; Saponins/pharmacology ; Saponins/therapeutic use ; Signal Transduction ; Muscular Atrophy/drug therapy ; Muscular Atrophy/prevention & control
Chemical Substances	Plant Extracts ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Saponins
Language	English
Publishing date	2023-04-06
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116458
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Zs.A 1494: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Phthalocyanine blue leaching and exposure effects on Microcystis aeruginosa (cyanobacteria) of photoaged microplastics.

Zeng, Ganning / Dai, Mengzheng / Liu, Peirui / Chen, Tiansheng / Hu, Lingling / Luo, Hongwei / Zhou, Qian / Du, Mingming / Pan, Xiangliang

Journal of hazardous materials

2024 Volume 469, Page(s) 133984

Abstract: Light-stabilizing additives may contribute to the overall pollution load of microplastics (MPs) and potentially enter the food chain, severely threatening aquatic life and human health. This study investigated the variation between polystyrene (PS) MPs ... ...

Abstract	Light-stabilizing additives may contribute to the overall pollution load of microplastics (MPs) and potentially enter the food chain, severely threatening aquatic life and human health. This study investigated the variation between polystyrene (PS) MPs and phthalocyanine blue (CuPC)-containing MPs before and after photoaging, as well as their effects on Microcystis aeruginosa. The presence of PS-MPs increased cell mortality, antioxidant enzyme activity, and the variation in extracellular components, while the presence of CuPC exacerbated these variations. CuPC-containing MPs caused different increasing trends in superoxide dismutase and malondialdehyde activities due to electron transfer across the membrane. Transcriptomic analysis revealed that the MPs and CuPC affected various cellular processes, with the greatest impact being on cell membranes. Compared with MPs, CuPC negatively affected ribosome and polysaccharide formation. These findings provide insights into the molecular mechanisms underlying the cellular response to MPs and their associated light-stabilizer pollution and imply the necessity for mitigating the pollution of both MPs and light-stabilizers.
MeSH term(s)	Humans ; Microcystis ; Microplastics/toxicity ; Plastics/toxicity ; Cyanobacteria ; Antioxidants ; Polystyrenes ; Water Pollutants, Chemical/toxicity ; Indoles ; Organometallic Compounds
Chemical Substances	copper phthalocyanine (3VEX9T7UT5) ; Microplastics ; Plastics ; Antioxidants ; Polystyrenes ; Water Pollutants, Chemical ; Indoles ; Organometallic Compounds
Language	English
Publishing date	2024-03-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2024.133984
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Article: Insights into forming behavior and CO2 adsorption properties of graphene from Sargassum horneri by Fe(NO3)3/KOH activation

Zeng, Ganning / Lou, Sa / Lou, Fengyan / Du, Mingming / Luo, Hongwei / Ai, Ning

Journal of chemical technology and biotechnology. 2022 Oct., v. 97, no. 10

2022

Abstract: BACKGROUND: Macroalgae biomass as a graphene source shows great potential at large scale in an energy shortage era. To achieve a more moderate and economic graphene synthesis method, porous graphenes were synthesized using Sargassum horneri as precursor, ...

Abstract	BACKGROUND: Macroalgae biomass as a graphene source shows great potential at large scale in an energy shortage era. To achieve a more moderate and economic graphene synthesis method, porous graphenes were synthesized using Sargassum horneri as precursor, in combination with Fe(NO₃)₃ and KOH composite. RESULTS: The addition of 10 wt% Fe(NO₃)₃ obviously accelerated KOH activation and decreased KOH loading and reaction temperature. Meanwhile, the graphene synthesized by Fe(NO₃)₃/KOH had better regeneration performance than that activated merely by KOH, even under more moderate thermal condition. The porous graphene displayed high specific surface area (~2311 m² g⁻¹) and exhibited good CO₂ uptake capacity (~4.39 mmol g⁻¹) at 30 °C and 1 bar. The CO₂ adsorption rate equations of the graphene were consistent with the intraparticle diffusion model, indicating that CO₂ adsorption was mainly controlled by the pore structure and additionally influenced by the chemical functional groups. CONCLUSION: The present work focuses on the high value‐added comprehensive utilization of macroalgae, which not only has important significance for on‐scale preparation of bio‐originated graphene and CO₂ adsorption, but also has positive benefits for macroalgae resource development. © 2022 Society of Chemical Industry (SCI).
Keywords	Sargassum horneri ; adsorption ; biomass ; biotechnology ; carbon dioxide ; energy ; graphene ; macroalgae ; models ; resource management ; surface area ; temperature ; value added
Language	English
Dates of publication	2022-10
Size	p. 2844-2851.
Publishing place	John Wiley & Sons, Ltd.
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1479465-2
ISSN	1097-4660 ; 0268-2575
ISSN (online)	1097-4660
ISSN	0268-2575
DOI	10.1002/jctb.7155
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Bioremediation of clay with high oil content and biological response after restoration.

Li, Xiaokang / Li, Jinling / Qu, Chengtun / Yu, Tao / Du, Mingming

Scientific reports

2021 Volume 11, Issue 1, Page(s) 9725

Abstract: The clay with high oil content form soil lumps, which is hard for microbes to repair. In this paper, the bioaugmentation and biostimulation technology were applied to improve the bioremediation effect of the soil with high oil content, that modified by ... ...

Abstract	The clay with high oil content form soil lumps, which is hard for microbes to repair. In this paper, the bioaugmentation and biostimulation technology were applied to improve the bioremediation effect of the soil with high oil content, that modified by local cow dung and sandy soil, the ecological toxicity of the soil after restoration was further analyzed. After 53 days of bioremediation, the degradation efficiency with respect to the total petroleum hydrocarbons (TPH) content reached 76.9% ± 2.2%. The soil bacterial count of M5 group reached log10 CFU/g soil = 7.69 ± 0.03 and the results were better than other experimental groups. The relative abundances of petroleum-degrading bacteria added to M5 remained high (Achromobacter 9.44%, Pseudomonas 31.06%, and Acinetobacter 14.11%), and the proportions of some other indigenous bacteria (Alcanivorax and Paenibacillus) also increased. The toxicity of the bioremediated soil was reduced by seed germination and earthworm survival experiments.
Language	English
Publishing date	2021-05-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-88033-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Tibio-Femoral Contact Force Distribution of Knee Before and After Total Knee Arthroplasty: Combined Finite Element and Gait Analysis.

Du, Mingming / Sun, Jun / Liu, Yancheng / Wang, Yingpeng / Yan, Songhua / Zeng, Jizhou / Zhang, Kuan

Orthopaedic surgery

2022 Volume 14, Issue 8, Page(s) 1836–1845

Abstract: Objective: To assess the tibio-femoral contact forces before and after total knee arthroplasty (TKA) in patients with knee osteoarthritis (KOA) by three-dimensional (3D) finite element analysis (FEA) models and gait analysis.: Methods: Two ... ...

Abstract	Objective: To assess the tibio-femoral contact forces before and after total knee arthroplasty (TKA) in patients with knee osteoarthritis (KOA) by three-dimensional (3D) finite element analysis (FEA) models and gait analysis. Methods: Two hospitalized patients with Kellgren-Lawrence grade IV varus KOA and two healthy subjects were enrolled in this study. Both patients underwent unilateral TKA. FEA models were established based on CT and MR images of the knees of the patients with KOA and healthy subjects. Gait analysis was performed using a three-dimensional motion capture system with a force plate. Three direction forces at the ankle joints were calculated by inverse dynamic analysis, which provided the load for the FEA models. The total contact forces of the knee joints were also calculated by inverse dynamic analysis to enable comparisons with the results from the FEA models. The total knee contact forces, maximum von Mises stress, and stress distribution of the medial plateau were compared between the patients and healthy subjects. The distributions of the medial plateau force at 2 and 6 months postoperatively were compared with the distributions of the forces preoperatively and those in the healthy subjects. Results: During static standing, the medial plateau bore the most of the total contact forces in the knees with varus KOA (90.78% for patient 1 and 93.53% for patient 2) compared with 64.75 ± 3.34% of the total force in the healthy knees. At the first and second peaks of the ground reaction force during the stance phase of a gait cycle, the medial plateau bore a much higher percentage of contact forces in patients with KOA (74.78% and 86.48%, respectively, for patient 1; 70.68% and 83.56%, respectively, for patient 2) than healthy subjects (61.06% ± 3.43% at the first peak and 72.09% ± 1.83% at the second peak). Two months after TKA, the percentages of contact forces on the medial tibial plateau were 79.65%-85.19% at the first and second peaks of ground reaction forces during the stance phase of a gait cycle, and the percentages decreased to 53.99% - 68.13% 6 months after TKA. Conclusion: FEA showed that TKA effectively restored the distribution of tibio-femoral contact forces during static standing and walking, especially 6 months after the surgery. The changes in the gait were consistent with the changes in the contact force distribution calculated by the FEA model.
MeSH term(s)	Arthroplasty, Replacement, Knee/methods ; Biomechanical Phenomena ; Finite Element Analysis ; Gait ; Gait Analysis ; Humans ; Knee Joint/diagnostic imaging ; Knee Joint/surgery ; Osteoarthritis, Knee/surgery
Language	English
Publishing date	2022-06-29
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2503162-4
ISSN	1757-7861 ; 1757-7853 ; 1757-7861
ISSN (online)	1757-7861 ; 1757-7853
ISSN	1757-7861
DOI	10.1111/os.13361
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Zs.A 6786: Show issues

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Article ; Online: Deep convolutional neural networks for aged microplastics identification by Fourier transform infrared spectra classification.

Zeng, Ganning / Ma, Yuan / Du, Mingming / Chen, Tiansheng / Lin, Liangyu / Dai, Mengzheng / Luo, Hongwei / Hu, Lingling / Zhou, Qian / Pan, Xiangliang

The Science of the total environment

2023 Volume 913, Page(s) 169623

Abstract: Infrared (IR) spectroscopy is a powerful technique for detecting and identifying Microplastics (MPs) in the environment. However, the aging of MPs presents a challenge in accurately identification and classification. To address this challenge, a ... ...

Abstract	Infrared (IR) spectroscopy is a powerful technique for detecting and identifying Microplastics (MPs) in the environment. However, the aging of MPs presents a challenge in accurately identification and classification. To address this challenge, a classification model based on deep convolutional neural networks (CNNs) was developed using infrared spectra results. Particularly, original infrared (IR) spectra were used as the sample dataset, therefore, relevant spectral details were preserved and additional noise or distortions were not introduced. The Adam (Adaptive moment estimation) algorithm was employed to accelerate gradient descent and weight update, the Dropout function was implemented to prevent overfitting and enhance the generalization performance of the network. An activation function ReLu (Rectified Linear Unit) was also utilized to simplify the co-adaptation relationship among neurons and prevent gradient disappearance. The performance of the CNN model in MPs classification was evaluated based on accuracy and robustness, and compared with other machine learning techniques. CNN model demonstrated superior capabilities in feature extraction and recognition, and greatly simplified the pre-processing procedure. The identification results of aged commercial microplastic samples showed accuracies of 40 % for Artificial Neural Network, 60 % for Random Forest, 80 % for Deep Neural Network, and 100 % for CNN, respectively. The CNN architecture developed in this work also demonstrates versatility by being suitable for both limited data cases and potential expansion to include more discrete data in the future.
Language	English
Publishing date	2023-12-28
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.169623
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