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  1. Article: [Polymorphism analysis of 20 autosomal short-tandem repeat loci in southern Chinese Han population].

    Chen, Ling / Lu, Hui-Jie / DU, Wei-An / Qiu, Ping-Ming / Liu, Chao

    Nan fang yi ke da xue xue bao = Journal of Southern Medical University

    2017  Volume 37, Issue 2, Page(s) 141–149

    Abstract: Objective: To evaluate the value of PowerPlex: Methods: We conducted genotyping experiments using PowerPlex: Results: The PowerPlex: Conclusion: The 20 STR loci are highly polymorphic in this southern Chinese Han population, suggesting the ... ...

    Abstract Objective: To evaluate the value of PowerPlex
    Methods: We conducted genotyping experiments using PowerPlex
    Results: The PowerPlex
    Conclusion: The 20 STR loci are highly polymorphic in this southern Chinese Han population, suggesting the value of this set of STR loci in forensic personal identification, paternity testing and anthropological study.
    MeSH term(s) Asian Continental Ancestry Group/genetics ; China ; Cluster Analysis ; Ethnic Groups ; Forensic Sciences ; Gene Frequency ; Genetics, Population ; Genotype ; Humans ; Microsatellite Repeats ; Polymorphism, Genetic
    Language Chinese
    Publishing date 2017-02-16
    Publishing country China
    Document type Journal Article
    ZDB-ID 2250951-3
    ISSN 1673-4254
    ISSN 1673-4254
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Efficiency of 27-plex single nucleotide polymorphism multiplex system for ancestry inference in different populations].

    Feng, Xing-Ling / Sun, Qi-Fan / Liu, Hong / Wei, Yi-Liang / DU, Wei-An / Li, Cai-Xia / Chen, Ling / Liu, Chao

    Nan fang yi ke da xue xue bao = Journal of Southern Medical University

    2017  Volume 37, Issue 4, Page(s) 555–562

    Abstract: Objective: To validate the efficiency of 27-plex single nucleotide polymorphism (SNP) multiplex system for ancestry inference.: Methods: The 27-plex SNP system was validated for its sensitivity and species specificity. A total of 533 samples were ... ...

    Abstract Objective: To validate the efficiency of 27-plex single nucleotide polymorphism (SNP) multiplex system for ancestry inference.
    Methods: The 27-plex SNP system was validated for its sensitivity and species specificity. A total of 533 samples were collected from African, Southern Chinese Han, China's ethic minorities (Yi, Hui, Miao, Tibet, and Uygur), European, Central Asian, Western Asian, Southern Asian, Southeast Asian and South American populations for clustering analysis of the genotypes by citing 3 representative continental ancestral groups [East Asia (CHB), Europe (CEU), and Africa (YRI)] from HapMap database.
    Results: The system sensitivity is 0.125 ng. Twenty and six genotypes were detected in chimpanzee and monkeys, respectively. Except in rs10496971, no more products were found in other animals. The system was capable of differentiating intercontinental populations but not of distinguishing between East Asian and Southeast Asian population or between Southern Chinese Han population and Chinese Ethnic populations (Hui, Miao, Yi and Tibet). This system achieved a 100% accuracy for intercontinental population source inference for 46 blind test samples.
    Conclusion: 27-plex SNPs multiplex system has a high sensitivity and species specificity and can correctly differentiate the ancestry origins of individuals from African, European and East Asian for criminal case investigation. But this system is not capable of distinguishing subpopulation groups and more specific ancestry-informative markers are needed to improve its recognition of Southeast Asian and Chinese ethnic populations.
    MeSH term(s) Animals ; Asian Continental Ancestry Group/genetics ; China ; Ethnic Groups ; Gene Frequency ; Genetics, Population ; Genotype ; Humans ; Polymorphism, Single Nucleotide ; Primates/genetics
    Language Chinese
    Publishing date 2017-04-26
    Publishing country China
    Document type Journal Article
    ZDB-ID 2250951-3
    ISSN 1673-4254
    ISSN 1673-4254
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: [Genetic polymorphism of eleven canine STR loci].

    Du, Wei-An / Xiong, Yong-Hua / Ye, Jun-Hua / Yang, Qian-Yong

    Fa yi xue za zhi

    2008  Volume 24, Issue 6, Page(s) 425–428

    Abstract: Objective: To investigate the polymorphism of 11 canine STR loci.: Methods: A fluorescent multiplex system with 11 STR loci (PEZ1, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, FH2010, FH2054, FH2132 and FH2611) was constructed independently and performed to ... ...

    Abstract Objective: To investigate the polymorphism of 11 canine STR loci.
    Methods: A fluorescent multiplex system with 11 STR loci (PEZ1, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, FH2010, FH2054, FH2132 and FH2611) was constructed independently and performed to amplify 105 samples from dogs. The character of these loci was analyzed with the PCR data.
    Results: The distributions of genotypes and allele frequencies of 11 STR loci were obtained. The total power of discrimination for the 11 loci in canine population was 0.9999999 and the cumulative probability of exclusion was 0.9330621. The observed heterozygosity and polymorphism information content (PIC) were 0.502 and 0.640, respectively.
    Conclusion: Each of the eleven canine STR loci has a high genetic polymorphism and can be applied for the parentage testing and individual identification. The fluorescent multiplex system is a reliable method in forensic application.
    MeSH term(s) Alleles ; Animals ; Dogs/genetics ; Forensic Genetics ; Gene Frequency ; Genotype ; Microsatellite Repeats/genetics ; Polymorphism, Genetic
    Language Chinese
    Publishing date 2008-12
    Publishing country China
    Document type English Abstract ; Journal Article
    ISSN 1004-5619
    ISSN 1004-5619
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Developmental validation of the AGCU 21+1 STR kit: a novel multiplex assay for forensic application.

    Zhu, Bo-Feng / Zhang, Yu-Dang / Shen, Chun-Mei / Du, Wei-An / Liu, Wen-Juan / Meng, Hao-Tian / Wang, Hong-Dan / Yang, Guang / Jin, Rui / Yang, Chun-Hua / Yan, Jiang-Wei / Bie, Xiao-Hua

    Electrophoresis

    2015  Volume 36, Issue 2, Page(s) 271–276

    Abstract: In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, ... ...

    Abstract In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.
    MeSH term(s) Animals ; DNA Primers ; Forensic Sciences/methods ; Humans ; Magnesium ; Microsatellite Repeats ; Multiplex Polymerase Chain Reaction/methods ; Reagent Kits, Diagnostic ; Sensitivity and Specificity ; Sex Determination Analysis ; Species Specificity ; Taq Polymerase ; Temperature
    Chemical Substances DNA Primers ; Reagent Kits, Diagnostic ; Taq Polymerase (EC 2.7.7.-) ; Magnesium (I38ZP9992A)
    Language English
    Publishing date 2015-01
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Validation Studies
    ZDB-ID 619001-7
    ISSN 1522-2683 ; 0173-0835
    ISSN (online) 1522-2683
    ISSN 0173-0835
    DOI 10.1002/elps.201400333
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1868: Show issues Location:
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  5. Article: Developmental validation of the AGCU 21+1 STR kit: A novel multiplex assay for forensic application

    Zhu, Bo‐Feng / Zhang, Yu‐Dang / Shen, Chun‐Mei / Du, Wei‐An / Liu, Wen‐Juan / Meng, Hao‐Tian / Wang, Hong‐Dan / Yang, Guang / Jin, Rui / Yang, Chun‐Hua / Yan, Jiang‐Wei / Bie, Xiao‐Hua

    Electrophoresis. 2015 Jan., v. 36, no. 2

    2015  

    Abstract: In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex‐determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, ... ...

    Abstract In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex‐determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.
    Keywords DNA ; electrophoresis ; forensic sciences ; guidelines ; loci ; polymerase chain reaction
    Language English
    Dates of publication 2015-01
    Size p. 271-276.
    Publishing place Verlag Chemie
    Document type Article
    ZDB-ID 619001-7
    ISSN 1522-2683 ; 0173-0835
    ISSN (online) 1522-2683
    ISSN 0173-0835
    DOI 10.1002/elps.201400333
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Diversity study of 12 X-chromosomal STR loci in Hui ethnic from China.

    Meng, Hao-Tian / Han, Jun-Tao / Zhang, Yu-Dang / Liu, Wen-Juan / Wang, Tian-Ju / Yan, Jiang-Wei / Huang, Jing-Feng / Du, Wei-An / Guo, Jian-Xin / Wang, Hong-Dan / Zhang, Yu-Hong / Zhou, Ru-Hua / Zhu, Bo-Feng / Wei, Xing

    Electrophoresis

    2014  Volume 35, Issue 14, Page(s) 2001–2007

    Abstract: X-chromosomal STRs (X-STRs) have been used as complements of autosomal STR application in recent years. In this work, we present population genetic data of 12 X-STRs including DXS101, DXS10159, DXS10162, DXS10164, DXS6789, DXS7133, DXS7423, DXS7424, ... ...

    Abstract X-chromosomal STRs (X-STRs) have been used as complements of autosomal STR application in recent years. In this work, we present population genetic data of 12 X-STRs including DXS101, DXS10159, DXS10162, DXS10164, DXS6789, DXS7133, DXS7423, DXS7424, DXS8378, DXS981, GATA165B12, and GATA31E08 loci in a sample of 231 unrelated healthy individuals from the Hui ethnic group in Ningxia Hui Autonomous Region, China. Allelic frequencies of the 12 X-STR loci and haplotypic frequencies of the reported linkage groups (DXS7424-DXS101 and DXS10159-DXS10164-DXS10162) were investigated in the group, respectively. No STR loci showed significant deviations from the Hardy-Weinberg equilibriums and no linkage disequilibriums of pairwise loci were found after Bonferroni correction, respectively. A combined power of discrimination in female individuals was 0.999999999985 and that in male individuals was 0.99999967, respectively. The combined mean exclusion chance in deficiency cases, normal trios and duo cases were 0.999934, 0.995754, and 0.999796, respectively. Significant differences were observed from 0 to 8 loci, when making comparisons between the data of Hui ethnic group and previously reported data from other 16 populations. The results indicated the new panel of 12 X-STR loci might be useful for forensic science application.
    MeSH term(s) Asian Continental Ancestry Group/genetics ; China ; Chromosomes, Human, X ; Ethnic Groups/genetics ; Female ; Genetics, Population/methods ; Genotyping Techniques ; Haplotypes ; Humans ; Linkage Disequilibrium ; Male ; Microsatellite Repeats ; Polymorphism, Genetic
    Language English
    Publishing date 2014-07
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 619001-7
    ISSN 1522-2683 ; 0173-0835
    ISSN (online) 1522-2683
    ISSN 0173-0835
    DOI 10.1002/elps.201400045
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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