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Article ; Online: Binding capacity and co-migration potential of Pb(II), Cu(II), and Cd(II) on colloids in road runoff.

Du, Xiaoli / Yu, Xinhong / Liang, Hui / Cui, Shenshen / Chi, Zhongwen

Environmental technology

2024 , Page(s) 1–12

Abstract: To evaluate the co-migration potential between heavy metal ions and road runoff colloids, the influence of contact time, temperature, initial concentration of metal ions, pH, humic acid (HA), and polymetallic coexistence on the binding capacity of heavy ... ...

Abstract	To evaluate the co-migration potential between heavy metal ions and road runoff colloids, the influence of contact time, temperature, initial concentration of metal ions, pH, humic acid (HA), and polymetallic coexistence on the binding capacity of heavy metals onto runoff colloids were investigated. The adsorption of heavy metals by runoff colloids was extremely rapid, approximately 80% of the equilibrium adsorption capacity was achieved in the first 30 min. The binding capacity exhibited an increasing trend with the initial concentration of metal ions increasing, and the maximum adsorption capacities of Pb(II), Cu(II), and Cd(II) achieved 159.13, 56.06, and 78.35 mg/g at 298 K, respectively. The adsorption capacity of Cu(II) and Cd(II) by runoff colloids increased with temperature increasing, while it displayed a converse trend for Pb(II). Neutral pH facilitated the combination of metal ions and runoff colloids. The presence of humic acid increased the binding capacity of Pb(II), Cu(II), and Cd(II) onto runoff colloids by 72.19, 63.31, and 13.83mg/g, respectively. Compared to the monometallic systems, the binding capacity of Pb(II), Cu(II), and Cd(II) by runoff colloids decreased by 18.44%, 22.35%, and 56.06% in polymetallic systems, respectively. Pb(II) bounded with colloids in the road runoff should be controlled preferentially to avoid their migrations to aquatic environments.
Language	English
Publishing date	2024-01-17
Publishing country	England
Document type	Journal Article
ISSN	1479-487X
ISSN (online)	1479-487X
DOI	10.1080/09593330.2024.2304672
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Article ; Online: Development and validation of an HPLC method for quantification of dolutegravir in human plasma.

Li, Dongsheng / Fu, Qiang / Du, Xiaoli / Li, Taisheng

Biomedical chromatography : BMC

2023 Volume 37, Issue 10, Page(s) e5708

Abstract: Dolutegravir (DTG) has been the first-line drug in many human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) guidelines for the treatment of naïve and experienced HIV-infected individuals, which calls for cost-effective and ... ...

Abstract	Dolutegravir (DTG) has been the first-line drug in many human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) guidelines for the treatment of naïve and experienced HIV-infected individuals, which calls for cost-effective and convenient methods for quantitative detection of DTG in human plasma for pharmacokinetic studies and patient adherence evaluation. Here, an HPLC-ultraviolet method in combination with liquid-liquid extraction with isocratic elution was developed for the first time. The analysis was performed on a CLC-ODS column (6 mm internal diameter × 15 cm, 5 μm) using a mixture of acetonitrile and phosphate buffer (40:60, v/v) as the mobile phase at the flow rate of 1 mL/min. Using triamcinolone as the internal standard, 100 μL of plasma sample was extracted by methyl tert-butyl ether, followed by evaporating under nitrogen stream, re-dissolving with 100 μL mobile phase, and injection of 20-40 μL of supernatant into the chromatographic system. The linearity of DTG was good in the range of 0.05-10 μg/mL (r = 0.9995), and the inter- and intra-day variabilities were 0.4%-4.3% (n = 10) and 1.2%-6.2% (n = 10) for the lower limit of quantification, low-, medium-, and high-concentration quality control samples (0.05, 0.1, 0.8, and 8 μg/mL), respectively, while the methodological and extraction recoveries were 98.0%-103.0% (n = 20) and 65.2%-75.7% (n = 3), respectively. This method was successfully applied to analyze DTG plasma concentration in 84 Chinese patients with HIV.
MeSH term(s)	Humans ; Chromatography, High Pressure Liquid ; Heterocyclic Compounds, 3-Ring ; Oxazines ; HIV Infections
Chemical Substances	dolutegravir (DKO1W9H7M1) ; Heterocyclic Compounds, 3-Ring ; Oxazines
Language	English
Publishing date	2023-08-22
Publishing country	England
Document type	Journal Article
ZDB-ID	632848-9
ISSN	1099-0801 ; 0269-3879
ISSN (online)	1099-0801
ISSN	0269-3879
DOI	10.1002/bmc.5708
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Article: hPSC-derived lung organoids: Potential opportunities and challenges.

Du, Xiaoli / Dong, Yongpin / Li, Wenfang / Chen, Yong

Heliyon

2023 Volume 9, Issue 2, Page(s) e13498

Abstract: Three-dimensional hPSC-derived lung organoids resemble the fetal lung stage, making them an excellent model for studying human lung development. However, current hPSC-derived lung organoids remain incomplete as they lack native lung components such as ... ...

Abstract	Three-dimensional hPSC-derived lung organoids resemble the fetal lung stage, making them an excellent model for studying human lung development. However, current hPSC-derived lung organoids remain incomplete as they lack native lung components such as vasculature, neurons and immune cells. This highlights the need to generate more complex hPSC-derived lung organoids that can faithfully mimic native human lungs for studying human lung development, regeneration, disease modeling and drug screen. In this review, we will discuss the current studies related to the generation of hPSC-derived lung organoids, highlighting how hPSC-derived lung organoids can contribute to the understanding of human lung development. We further focus on potential approaches to generate more complex hPSC-derived lung organoids containing native cellular components. Finally, we discuss the present limitations and potential applications of hPSC-derived lung organoids in the future.
Language	English
Publishing date	2023-02-04
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e13498
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Article ; Online: Changes in healthy effects and economic burden of PM

Chen, Fengxia / Wang, Yan / Du, Xiaoli

Environmental science and pollution research international

2023 Volume 30, Issue 21, Page(s) 60294–60302

Abstract: The COVID-19 lockdown had a positive control effect on urban air quality. However, this effect remains uncertain after the epidemic enters regular management, and furthermore, only limited data are available regarding urban ... ...

Abstract	The COVID-19 lockdown had a positive control effect on urban air quality. However, this effect remains uncertain after the epidemic enters regular management, and furthermore, only limited data are available regarding urban PM
MeSH term(s)	Humans ; Beijing/epidemiology ; Air Pollutants/analysis ; Particulate Matter/analysis ; Financial Stress ; COVID-19/epidemiology ; Communicable Disease Control ; Air Pollution/analysis ; China/epidemiology
Chemical Substances	Air Pollutants ; Particulate Matter
Language	English
Publishing date	2023-04-06
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-023-26005-5
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Article ; Online: RNNCon: Contribution Coverage Testing for Stacked Recurrent Neural Networks.

Du, Xiaoli / Zeng, Hongwei / Chen, Shengbo / Lei, Zhou

Entropy (Basel, Switzerland)

2023 Volume 25, Issue 3

Abstract: Recurrent Neural Networks (RNNs) are applied in safety-critical fields such as autonomous driving, aircraft collision detection, and smart credit. They are highly susceptible to input perturbations, but little research on RNN-oriented testing techniques ... ...

Abstract	Recurrent Neural Networks (RNNs) are applied in safety-critical fields such as autonomous driving, aircraft collision detection, and smart credit. They are highly susceptible to input perturbations, but little research on RNN-oriented testing techniques has been conducted, leaving a threat to a large number of sequential application domains. To address these gaps, improve the test adequacy of RNNs, find more defects, and improve the performance of RNNs models and their robustness to input perturbations. We aim to propose a test coverage metric for the underlying structure of RNNs, which is used to guide the generation of test inputs to test RNNs. Although coverage metrics have been proposed for RNNs, such as the hidden state coverage in RNN-Test, they ignore the fact that the underlying structure of RNNs is still a fully connected neural network but with an additional "delayer" that records the network state at the time of data input. We use the contributions, i.e., the combination of the outputs of neurons and the weights they emit, as the minimum computational unit of RNNs to explore the finer-grained logical structure inside the recurrent cells. Compared to existing coverage metrics, our research covers the decision mechanism of RNNs in more detail and is more likely to generate more adversarial samples and discover more flaws in the model. In this paper, we redefine the contribution coverage metric applicable to Stacked LSTMs and Stacked GRUs by considering the joint effect of neurons and weights in the underlying structure of the neural network. We propose a new coverage metric, RNNCon, which can be used to guide the generation of adversarial test inputs. And we design and implement a test framework prototype RNNCon-Test. 2 datasets, 4 LSTM models, and 4 GRU models are used to verify the effectiveness of RNNCon-Test. Compared to the current state-of-the-art study RNN-Test, RNNCon can cover a deeper decision logic of RNNs. RNNCon-Test is not only effective in identifying defects in Deep Learning (DL) systems but also in improving the performance of the model if the adversarial inputs generated by RNNCon-Test are filtered and added to the training set to retrain the model. In the case where the accuracy of the model is already high, RNNCon-Test is still able to improve the accuracy of the model by up to 0.45%.
Language	English
Publishing date	2023-03-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2014734-X
ISSN	1099-4300 ; 1099-4300
ISSN (online)	1099-4300
ISSN	1099-4300
DOI	10.3390/e25030520
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Article ; Online: hPSC-derived lung organoids: Potential opportunities and challenges

Du, Xiaoli / Dong, Yongpin / Li, Wenfang / Chen, Yong

Heliyon. 2023 Feb., v. 9, no. 2 p.e13498-

2023

Abstract	Three-dimensional hPSC-derived lung organoids resemble the fetal lung stage, making them an excellent model for studying human lung development. However, current hPSC-derived lung organoids remain incomplete as they lack native lung components such as vasculature, neurons and immune cells. This highlights the need to generate more complex hPSC-derived lung organoids that can faithfully mimic native human lungs for studying human lung development, regeneration, disease modeling and drug screen. In this review, we will discuss the current studies related to the generation of hPSC-derived lung organoids, highlighting how hPSC-derived lung organoids can contribute to the understanding of human lung development. We further focus on potential approaches to generate more complex hPSC-derived lung organoids containing native cellular components. Finally, we discuss the present limitations and potential applications of hPSC-derived lung organoids in the future.
Keywords	drugs ; humans ; lungs ; models ; organoids ; Lung ; Organoid ; hPSC ; Development ; Vasculature ; Neurons
Language	English
Dates of publication	2023-02
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e13498
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Article ; Online: A preliminary study on plasma concentration, short-term efficacy, and safety profile of dolutegravir in Chinese people with HIV.

Li, Dongsheng / Xiao, Ran / Fu, Qiang / Song, Xiaojing / Han, Yang / Du, Xiaoli / Li, Taisheng

Expert review of clinical pharmacology

2024 , Page(s) 1–7

Abstract: Background: This study examined the plasma concentration, clinical efficacy, and safety of dolutegravir (DTG) in Chinese people with HIV (PWH).: Methods: In this observational study, HIV-positive individuals on DTG-based regimens for at least 6 ... ...

Abstract	Background: This study examined the plasma concentration, clinical efficacy, and safety of dolutegravir (DTG) in Chinese people with HIV (PWH). Methods: In this observational study, HIV-positive individuals on DTG-based regimens for at least 6 months were included. Plasma DTG concentrations were measured 1 month after initiating treatment. Viral loads (VL) and CD4+ T cell counts were evaluated at baseline and after 1 and 6 months of therapy. High-performance liquid chromatography was used for measuring DTG concentrations, polymerase chain reaction for VL, and flow cytometry for CD4+ T cell counts. Safety assessments included monitoring liver enzymes, serum creatinine estimated glomerular filtration rate, and adverse reactions. Results: Eighty-two Chinese PWH were enrolled. Average VL decreased significantly from baseline by 3.1 log at 1 month and 3.5 log at 6 months. CD4+ T cell counts increased from 273 cells/mm Conclusion: Preliminary findings suggest higher DTG plasma concentrations in Chinese PWH compared to Western populations, with promising short-term efficacy and safety.
Language	English
Publishing date	2024-01-22
Publishing country	England
Document type	Journal Article
ISSN	1751-2441
ISSN (online)	1751-2441
DOI	10.1080/17512433.2024.2308664
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Article ; Online: Scarcity mindset impairs distractor suppression but not target enhancement in selective attention: Evidence from ERPs.

Zhang, Lijie / Xu, Mengsi / Du, Xiaoli / Yang, Qian / Qiao, Lei

Neuropsychologia

2023 Volume 191, Page(s) 108739

Abstract: A scarcity mindset, induced by a perception of material scarcity, profoundly influences how individuals allocate their attention. Selective attention, a crucial cognitive control process enabling individuals to focus on relevant information while ... ...

Abstract	A scarcity mindset, induced by a perception of material scarcity, profoundly influences how individuals allocate their attention. Selective attention, a crucial cognitive control process enabling individuals to focus on relevant information while ignoring distractions, holds significant importance in both professional and personal domains. However, the specific impact of a scarcity mindset on selective attention and its underlying mechanisms remains uncertain. The objective of this study is to examine the specific impact of a scarcity mindset on selective attention using an experimental approach. Participants were subjected to either scarcity or neutral conditions, and their brain activity was measured using Electroencephalography (EEG) while performing a visual search task. The task involved identifying the direction of a target while disregarding a distractor, which was presented either laterally or in conjunction with the target along the vertical midline. The findings of our study indicate that individuals with a scarcity mindset displayed compromised selective attention, as evidenced by longer response times and weaker N2pc amplitudes in EEG recordings. Notably, distractor suppression was compromised, as evidenced by diminished Pd amplitudes, whereas the enhancement of targets remained relatively unaltered, as indicated by similar Nt amplitudes. These findings highlight a reduced ability to filter out irrelevant information in individuals with a scarcity mindset, thereby holding significant implications for social policies and practices.
MeSH term(s)	Humans ; Evoked Potentials/physiology ; Attention/physiology ; Electroencephalography ; Reaction Time/physiology ; Mental Processes
Language	English
Publishing date	2023-11-24
Publishing country	England
Document type	Journal Article
ZDB-ID	207151-4
ISSN	1873-3514 ; 0028-3932
ISSN (online)	1873-3514
ISSN	0028-3932
DOI	10.1016/j.neuropsychologia.2023.108739
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Article: [Qirong Tablets inhibits apoptosis of ovarian granulosa cells via PI3K/Akt/ HIF-1 signaling pathway].

Nan, Nan / DU, Xiao-Li / Chen, Miao / Luo, Jia-Qi

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

2023 Volume 48, Issue 17, Page(s) 4774–4781

Abstract: This study aims to observe the effect and explore the mechanism of Qirong Tablets in the treatment of premature ovarian insufficiency(POI) in mice via the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/hypoxia inducible factor 1(HIF-1) ... ...

Abstract	This study aims to observe the effect and explore the mechanism of Qirong Tablets in the treatment of premature ovarian insufficiency(POI) in mice via the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/hypoxia inducible factor 1(HIF-1) signaling pathway. Sixty SPF female BALB/c mice were randomly divided into normal group, model group, positive control group, Qirong Tablets low-, medium-and high-dose group. The normal group was intraperitoneally injected with the same amount of normal saline, and the other groups were intraperitoneally injected with cyclophosphamide 120 mg·kg~(-1)·d~(-1) once to establish a POI animal model. After the model was successfully established, the low-, medium-and high-dose groups of Qirong Tablets were administered orally with 0.6, 1.2, 2.4 mg·kg~(-1)·d~(-1) respectively. The positive control group was given 0.22 mg·kg~(-1)·d~(-1) Clementine Tablets by intragastric administration, and the normal group and model group were given intragastric administration with the same amount of normal saline, and the treatment was 28 d as a course of treatment. After drug intervention, enzyme-linked immunosorbent assay(ELISA) was employed to measure the levels of estradiol(E_2), follicle-stimulating hormone(FSH), luteinizing hormone(LH), and anti-mullerian hormone(AMH) in peripheral blood, and hematoxylin-eosin(HE) staining to observe the ovarian tissue. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay was used to detect the apoptosis of granulosa cells, and Western blot to determine the expression levels of B-cell lymphoma-2(Bcl-2), Bcl-2-associated X protein(Bax), caspase-3, PI3K, Akt, and HIF-1. Compared with the normal group, the modeling of POI caused loose or destroyed ovarian tissue with vacuolar structures, edema and fibrosis in the ovarian interstitium, disordered or loose arrangement of granulosa cells, and reduced normal follicles. Compared with the model group, drug interventions restored the ovarian tissue and follicles at all the development stages and reduced atretic follicles. Compared with the normal group, the modeling of POI lowered the serum level of E_2 and AMH(P<0.01), and elevated the level of FSH and LH(P<0.01). Compared with the model group, high-dose Qirong Tablets elevated the levels of E_2 and AMH(P<0.05), and lowered the levels of FSH and LH(P<0.05). Compared with the normal group, the modeling of POI up-regulated the protein levels of PI3K, Akt, HIF-1, Bax, and caspase-3 and down-regulated the protein level of Bcl-2 in the ovarian tissue(P<0.01). Compared with the model group, low-, medium-, and high-dose Qirong Tablets down-regulated the protein levels of PI3K, Akt, HIF-1, Bax, and caspase-3 proteins and up-regulated the protein level of Bcl-2 in the ovarian tissue(P<0.05). In conclusion, Qirong Tablets can up-regulate the expression Bcl-2, down-regulate the expression of Bax and caspase-3 in POI mice. Qirong Tablets may inhibit the apoptosis of follicular granulosa cells in mice, thereby delaying ovarian aging, improving reproductive axis function, and strengthening ovarian reserve capacity, which may be associated with the inhibition of PI3K/Akt/HIF-1 pathway.
MeSH term(s)	Humans ; Mice ; Female ; Animals ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins c-akt/metabolism ; bcl-2-Associated X Protein ; Phosphatidylinositol 3-Kinases/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Caspase 3/metabolism ; Saline Solution/pharmacology ; Saline Solution/therapeutic use ; Signal Transduction ; Granulosa Cells ; Primary Ovarian Insufficiency/drug therapy ; Follicle Stimulating Hormone/pharmacology ; Follicle Stimulating Hormone/therapeutic use ; Proto-Oncogene Proteins c-bcl-2/genetics ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Apoptosis
Chemical Substances	Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; bcl-2-Associated X Protein ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Caspase 3 (EC 3.4.22.-) ; Saline Solution ; Follicle Stimulating Hormone (9002-68-0) ; Proto-Oncogene Proteins c-bcl-2
Language	Chinese
Publishing date	2023-10-06
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1004649-5
ISSN	1001-5302 ; 0254-0029
ISSN	1001-5302 ; 0254-0029
DOI	10.19540/j.cnki.cjcmm.20230424.501
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Article: Diagnostic Accuracy of the Diffusion-Weighted Imaging Method Used in Association With the Apparent Diffusion Coefficient for Differentiating Between Primary Central Nervous System Lymphoma and High-Grade Glioma: Systematic Review and Meta-Analysis.

Du, Xiaoli / He, Yue / Lin, Wei

Frontiers in neurology

2022 Volume 13, Page(s) 882334

Abstract: Background: It is difficult to differentiate between a few primary central nervous system lymphoma (PCNSL) and high-grade glioma (HGG) using conventional magnetic resonance imaging techniques. The purpose of this study is to explore whether diffusion- ... ...

Abstract	Background: It is difficult to differentiate between a few primary central nervous system lymphoma (PCNSL) and high-grade glioma (HGG) using conventional magnetic resonance imaging techniques. The purpose of this study is to explore whether diffusion-weighted imaging (DWI) can be effectively used to differentiate between these two types of tumors by analyzing the apparent diffusion coefficient (ADC). Research design and methods: Data presented in Pubmed, Embase, Web of Science, Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang Database, and China Science and Technology Journal Database (CQVIP) were analyzed. High-quality literature was included, and the quality was evaluated using the quality assessment of diagnostic accuracy studies-2 (QUADAS-2) tool, and the studies were based on the inclusion and exclusion rules. The pooled sensitivity, pooled specificity, pooled positive likelihood ratio (PLR), pooled negative likelihood ratio (NLR), pooled diagnostic odds ratio ( Results: In total, eighteen high-quality studies were included. The pooled sensitivity was 0.82 (95% CI: 0.75-0.88), the pooled specificity was 0.87 (95% CI: 0.84-0.90), the pooled positive likelihood ratio was 6.49 (95% CI: 5.06-8.32), the pooled NLR was 0.21 (95% CI: 0.14-0.30), the pooled Conclusions: The results obtained from the meta-analysis suggest that DWI is characterized by high diagnostic accuracy and thus can be effectively used for differentiating between PCNSL and HGG.
Language	English
Publishing date	2022-06-24
Publishing country	Switzerland
Document type	Systematic Review
ZDB-ID	2564214-5
ISSN	1664-2295
ISSN	1664-2295
DOI	10.3389/fneur.2022.882334
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