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  1. Article ; Online: Molecular mechanism of Hfq-dependent sRNA1039 and sRNA1600 regulating antibiotic resistance and virulence in Shigella sonnei.

    Du, Yazhe / Wang, Ya / Geng, Juan / Long, Jinzhao / Yang, Haiyan / Duan, Guangcai / Chen, Shuaiyin

    International journal of antimicrobial agents

    2023  Volume 63, Issue 2, Page(s) 107070

    Abstract: Bacillary dysentery caused by Shigella spp. is a significant concern for human health. Small non-coding RNA (sRNA) plays a crucial role in regulating antibiotic resistance and virulence in Shigella spp. However, the specific mechanisms behind this ... ...

    Abstract Bacillary dysentery caused by Shigella spp. is a significant concern for human health. Small non-coding RNA (sRNA) plays a crucial role in regulating antibiotic resistance and virulence in Shigella spp. However, the specific mechanisms behind this phenomenon are still not fully understood. This study discovered two sRNAs (sRNA1039 and sRNA1600) that may be involved in bacterial resistance and virulence. By constructing deletion mutants (WT/ΔSR1039 and WT/ΔSR1600), this study found that the WT/ΔSR1039 mutants caused a two-fold increase in sensitivity to ampicillin, gentamicin and cefuroxime, and the WT/ΔSR1600 mutants caused a two-fold increase in sensitivity to cefuroxime. Furthermore, the WT/ΔSR1600 mutants caused a decrease in the adhesion and invasion of bacteria to HeLa cells (P<0.01), and changed the oxidative stress level of bacteria to reduce their survival rate (P<0.001). Subsequently, this study explored the molecular mechanisms by which sRNA1039 and sRNA1600 regulate antibiotic resistance and virulence. The deletion of sRNA1039 accelerated the degradation of target gene cfa mRNA and reduced its expression, thereby regulating the expression of pore protein gene ompD indirectly and negatively to increase bacterial sensitivity to ampicillin, gentamicin and cefuroxime. The inactivation of sRNA1600 reduced the formation of persister cells to reduce resistance to cefuroxime, and reduced the expression of type-III-secretion-system-related genes to reduce bacterial virulence by reducing the expression of target gene tomB. These results provide new insights into Hfq-sRNA-mRNA regulation of the resistance and virulence network of Shigella sonnei, which could potentially promote the development of more effective treatment strategies.
    MeSH term(s) Humans ; Shigella sonnei/genetics ; Virulence/genetics ; HeLa Cells ; Cefuroxime/metabolism ; Shigella flexneri/genetics ; Shigella ; Dysentery, Bacillary/microbiology ; Ampicillin/pharmacology ; Ampicillin/metabolism ; Drug Resistance, Microbial ; Gentamicins ; RNA, Messenger ; RNA, Small Untranslated/genetics ; RNA, Small Untranslated/metabolism ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism
    Chemical Substances Cefuroxime (O1R9FJ93ED) ; Ampicillin (7C782967RD) ; Gentamicins ; RNA, Messenger ; RNA, Small Untranslated ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-12-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1093977-5
    ISSN 1872-7913 ; 0924-8579
    ISSN (online) 1872-7913
    ISSN 0924-8579
    DOI 10.1016/j.ijantimicag.2023.107070
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3368: Show issues Location:
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  2. Article ; Online: A meta-analysis of immunogenicity and safety of two versus single-doses of influenza A (H1N1) vaccine in person living with HIV.

    Zhang, Teng / Geng, Juan / Du, Yazhe / Yang, Haiyan / Jin, Yuefei / Chen, Shuaiyin / Duan, Guangcai

    International journal of STD & AIDS

    2023  Volume 35, Issue 5, Page(s) 326–336

    Abstract: Purpose: ...

    Abstract Purpose:
    MeSH term(s) Humans ; Adjuvants, Immunologic ; Antibodies, Viral ; Immunogenicity, Vaccine ; Influenza A Virus, H1N1 Subtype ; Influenza Vaccines/immunology ; Influenza, Human/prevention & control ; Vaccination ; HIV Infections
    Chemical Substances Adjuvants, Immunologic ; Antibodies, Viral ; Influenza Vaccines
    Language English
    Publishing date 2023-12-12
    Publishing country England
    Document type Meta-Analysis ; Journal Article ; Review
    ZDB-ID 1018089-8
    ISSN 1758-1052 ; 0956-4624
    ISSN (online) 1758-1052
    ISSN 0956-4624
    DOI 10.1177/09564624231220424
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 2829: Show issues Location:
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  3. Article ; Online: Association of cytotoxic T-lymphocyte antigen-4 (CTLA-4) single nucleotide polymorphism in donors with clinical outcome after allogeneic hematopoietic stem cell transplantation: a meta-analysis.

    Wang, Zhuo / Zhang, Yunwei / Du, Yazhe / Song, Fei / Gao, Sujun

    Hematology (Amsterdam, Netherlands)

    2021  Volume 26, Issue 1, Page(s) 144–152

    Abstract: Objective: Cytotoxic T-lymphocyte antigen 4 (CTLA-4) polymorphisms at positions of +49 and CT60 in donors have been reported to influence clinical outcome following allogeneic hematopoietic stem cell transplantation (allo-HSCT), such as overall survival ...

    Abstract Objective: Cytotoxic T-lymphocyte antigen 4 (CTLA-4) polymorphisms at positions of +49 and CT60 in donors have been reported to influence clinical outcome following allogeneic hematopoietic stem cell transplantation (allo-HSCT), such as overall survival (OS), disease free survival (DFS), relapse and the risk of graft versus host disease (GVHD). However, the results still remain controversial. Thus, we conducted the first meta-analysis to get a more accurate estimation of the relationship between CTLA-4 genotype and the above end points.
    Methods: PubMed, Embase, Web of science and Cochrane Library were searched to select eligible studies, data were extracted and then combined ORs/HRs together with the corresponding 95% confidence intervals (CIs) were calculated. Both the dominant and recessive models were employed to evaluate the associations between genetic variation in donor CTLA-4 and outcome after allo-HSCT.
    Results: A total of 15 studies were included the pooled results indicated that +49 GG homozygote in donors was significantly associated with increased risk of chronic GVHD (OR=1.701, 95% CI, 1.124-2.573,
    Conclusions: The present meta-analysis suggests that donors with CT60 G allele might be associated with worse OS but reduced severe aGVHD occurrence, while patients transplanted from donors with GG genotype at position of +49 are more likely to suffer from cGVHD.
    MeSH term(s) Alleles ; CTLA-4 Antigen/genetics ; Genotype ; Graft vs Host Disease/etiology ; Graft vs Host Disease/genetics ; Hematopoietic Stem Cell Transplantation/adverse effects ; Humans ; Polymorphism, Single Nucleotide ; Tissue Donors ; Transplantation, Homologous/adverse effects ; Treatment Outcome
    Chemical Substances CTLA-4 Antigen ; CTLA4 protein, human
    Language English
    Publishing date 2021-01-25
    Publishing country England
    Document type Journal Article ; Meta-Analysis ; Systematic Review
    ZDB-ID 1341428-8
    ISSN 1607-8454 ; 1024-5332 ; 1024-5340
    ISSN (online) 1607-8454
    ISSN 1024-5332 ; 1024-5340
    DOI 10.1080/16078454.2020.1852762
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4538: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Anxiety, depression, insomnia symptoms & associated factors among young to middle-aged adults during the resurgent epidemic of COVID-19: a cross-sectional study.

    Geng, Juan / Cheng, Cheng / Chen, Shuaiyin / Wang, Ya / Du, Yazhe / Long, Jinzhao / Jin, Yuefei / Yang, Haiyan / Duan, Guangcai

    Psychology, health & medicine

    2022  Volume 28, Issue 5, Page(s) 1336–1346

    Abstract: The coronavirus disease 2019 (COVID-19) pandemic is a public health emergency of international concern. However, its stress on the mental health of young to middle-aged adults is largely unexplored. This study aimed to evaluate the mental health ... ...

    Abstract The coronavirus disease 2019 (COVID-19) pandemic is a public health emergency of international concern. However, its stress on the mental health of young to middle-aged adults is largely unexplored. This study aimed to evaluate the mental health difficulties during the resurgent phase of COVID-19 among young to middle-aged adults in China. There were 1,478 participants with a median age of 26 years (IQR, 23 - 30), including 535 males (36.2%). The prevalence of anxiety, depression, and insomnia were 8.6%, 11.4%, and 13.7%, respectively. Participants aged 29 - 59 years (OR, 95% CI: 2.46, 1.23 - 4.91) and females (2.49, 1.55 - 4.01) had a higher risk of anxiety. Education status, worried level about the current COVID-19, and the level of COVID-19's impact on life were significantly associated with the prevalence of anxiety. Besides, the level of COVID-19's impact on life was positively related to the prevalence of depression and insomnia. Our study provided novel evidence of psychological difficulties among young to middle-aged adults during the resurgent stage of the COVID-19 epidemic. Psychological intervention should be continuously implemented to prevent long-term psychological comorbidities during the COVID-19 epidemic.
    MeSH term(s) Middle Aged ; Male ; Female ; Adult ; Humans ; Young Adult ; COVID-19 ; Cross-Sectional Studies ; Sleep Initiation and Maintenance Disorders ; Depression/psychology ; SARS-CoV-2 ; Surveys and Questionnaires ; Anxiety/psychology ; China/epidemiology
    Language English
    Publishing date 2022-11-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1477841-5
    ISSN 1465-3966 ; 1354-8506
    ISSN (online) 1465-3966
    ISSN 1354-8506
    DOI 10.1080/13548506.2022.2143542
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Measurable residual disease detected by flow cytometry independently predicts prognoses of NPM1-mutated acute myeloid leukemia.

    Zhang, Yun-Wei / Su, Long / Tan, Ye-Hui / Lin, Hai / Liu, Xiao-Liang / Liu, Qiu-Ju / Sun, Jing-Nan / Zhang, Ming / Du, Ya-Zhe / Song, Fei / Han, Wei / Gao, Su-Jun

    Annals of hematology

    2022  Volume 102, Issue 2, Page(s) 337–347

    Abstract: Acute myeloid leukemia (AML) with NPM1 mutation is a distinct genetic entity with favorable outcomes. Nevertheless, emerging evidence suggests that NPM1-mutated AML is still a highly heterogeneous disorder. In this study, 266 patients with AML with NPM1 ... ...

    Abstract Acute myeloid leukemia (AML) with NPM1 mutation is a distinct genetic entity with favorable outcomes. Nevertheless, emerging evidence suggests that NPM1-mutated AML is still a highly heterogeneous disorder. In this study, 266 patients with AML with NPM1 mutations were retrospectively analyzed to evaluate the associations between variant allele frequency (VAF) of NPM1 mutations, co-mutated genes, measurable residual disease (MRD), and patient outcomes. Multiparameter flow cytometry (MFC) and real-time quantitative polymerase chain reaction (RT-PCR) were used for monitoring MRD. Ultimately, 106 patients were included in the long-term follow-up period. Patients with high NPM1 VAF (≥ 42.43%) had poorer 2-year relapse-free survival (RFS) (55.7% vs. 70.2%, P = 0.017) and overall survival (OS) (63.7% vs. 82.0%, P = 0.027) than those with low VAF. DNMT3A mutations negatively influenced the outcomes of patients with NPM1 mutations. Patients with high DNMT3A VAF or NPM1/DNMT3A/FLT3-ITD triple mutations had shorter RFS and significantly lower OS than that in controls. After two cycles of chemotherapy, patients with positive MFC MRD results had lower RFS (MRD
    MeSH term(s) Humans ; Nuclear Proteins/genetics ; Nucleophosmin ; Retrospective Studies ; Flow Cytometry ; Prognosis ; Leukemia, Myeloid, Acute/diagnosis ; Leukemia, Myeloid, Acute/drug therapy ; Leukemia, Myeloid, Acute/genetics ; Recurrence ; Mutation ; Neoplasm, Residual/genetics
    Chemical Substances Nuclear Proteins ; Nucleophosmin (117896-08-9)
    Language English
    Publishing date 2022-11-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1064950-5
    ISSN 1432-0584 ; 0939-5555 ; 0945-8077
    ISSN (online) 1432-0584
    ISSN 0939-5555 ; 0945-8077
    DOI 10.1007/s00277-022-05033-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Uh III Zs.181: Show issues Location:
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  6. Article ; Online: Saikosaponin D exhibits anti-leukemic activity by targeting FTO/m

    Sun, Kaiju / Du, Yangyang / Hou, Yuzhu / Zhao, Mingyue / Li, Jiajia / Du, Yazhe / Zhang, Lingxiao / Chen, Changbao / Yang, Hongmei / Yan, Fei / Su, Rui

    Theranostics

    2021  Volume 11, Issue 12, Page(s) 5831–5846

    Abstract: Purpose: ...

    Abstract Purpose:
    MeSH term(s) Adenosine/analogs & derivatives ; Adenosine/metabolism ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO/metabolism ; Animals ; Apoptosis/drug effects ; Cell Cycle Checkpoints/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects ; HL-60 Cells ; Humans ; K562 Cells ; Leukemia, Myeloid, Acute/drug therapy ; Leukemia, Myeloid, Acute/metabolism ; Methylation/drug effects ; Mice ; Mice, Inbred C57BL ; Mice, Inbred NOD ; Mice, SCID ; Oleanolic Acid/analogs & derivatives ; Oleanolic Acid/pharmacology ; Protein Kinase Inhibitors/pharmacology ; Protein-Tyrosine Kinases/metabolism ; RNA, Messenger/genetics ; Saponins/pharmacology ; Signal Transduction/drug effects ; U937 Cells
    Chemical Substances Protein Kinase Inhibitors ; RNA, Messenger ; Saponins ; Oleanolic Acid (6SMK8R7TGJ) ; N-methyladenosine (CLE6G00625) ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO (EC 1.14.11.33) ; FTO protein, human (EC 1.14.11.33) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; Adenosine (K72T3FS567) ; saikosaponin D (UR635J3F00)
    Language English
    Publishing date 2021-03-31
    Publishing country Australia
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592097-2
    ISSN 1838-7640 ; 1838-7640
    ISSN (online) 1838-7640
    ISSN 1838-7640
    DOI 10.7150/thno.55574
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: [Clinical characteristics and therapeutic efficacy of normal karyotype AML patients with CEBPA mutation].

    Du, Ya-Zhe / Su, Long / Li, Wei / Yu, Ping / Tan, Ye-Hui / Lin, Hai / Gao, Su-Jun

    Zhongguo shi yan xue ye xue za zhi

    2014  Volume 22, Issue 1, Page(s) 16–19

    Abstract: This study was aimed to explore the clinical characteristics and therapeutic efficacy of normal karyotype AML patients with CEBPA mutations. Fifty-five de novo AML patients with normal karyotype were retrospectively analyzed with regard to frequency of ... ...

    Abstract This study was aimed to explore the clinical characteristics and therapeutic efficacy of normal karyotype AML patients with CEBPA mutations. Fifty-five de novo AML patients with normal karyotype were retrospectively analyzed with regard to frequency of CEBPA mutation, clinical characteristics and therapeutic response. The results showed that CEBPA mutation was detected in 20 patients (36.4%), among them 17 cases displayed double mutations, three cases were with single mutation. The clinical characteristics of patients with CEBPA mutation displayed as follows: 75% of AML patients with CEBPA mutation were AML-M1 and AML-M2, the hemoglobin level at newly diagnosis was higher and the platelet count at newly diagnosis time was lower than those of AML patients without CEBPA mutation [(98.30 ± 20.33) g/L vs (81.69 ± 23.74) g/L (P < 0.05); and (33.30 ± 38.27) ×10(9)/L vs (64.79 ± 61.60) ×10(9)/L (P < 0.05)]. The leukemic cells highly expressed CD7 and CD34. The therapeutic efficacy of 1 cycle for AML patients with CEBPA mutation was satisfactory (72.2%), was higher than that of patients without CEBPA mutation(68.6%), but there was no statistical significance (P > 0.05). It is concluded that AML with CEBPA mutation is more observed in AML-M1 and AML-M2, and accompanies by high level of hemoglobin and lower platelet count, expression of CD7 and CD34. Early-term therapeutic efficacy is satisfactory. The frequency of CEBPA mutation may be higher in Chinese patients with AML compared with that reported in Western world.
    MeSH term(s) Adolescent ; Adult ; CCAAT-Enhancer-Binding Proteins/genetics ; Child ; Female ; Humans ; Karyotype ; Karyotyping ; Leukemia, Myeloid, Acute/diagnosis ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/therapy ; Male ; Middle Aged ; Mutation ; Prognosis ; Retrospective Studies ; Treatment Outcome ; Young Adult
    Chemical Substances CCAAT-Enhancer-Binding Proteins ; CEBPA protein, human
    Language Chinese
    Publishing date 2014-02
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 2404306-0
    ISSN 1009-2137
    ISSN 1009-2137
    DOI 10.7534/j.issn.1009-2137.2014.01.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6603: Show issues Location:
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