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  1. Article ; Online: The buffer power of plasma proteins: experimental observations are consistent while the definitions are not.

    Krbec, Martin / Duška, František

    Journal of applied physiology (Bethesda, Md. : 1985)

    2023  Volume 134, Issue 4, Page(s) 992–993

    MeSH term(s) Blood Proteins ; Acid-Base Equilibrium
    Chemical Substances Blood Proteins
    Language English
    Publishing date 2023-04-07
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 219139-8
    ISSN 1522-1601 ; 0021-8987 ; 0161-7567 ; 8750-7587
    ISSN (online) 1522-1601
    ISSN 0021-8987 ; 0161-7567 ; 8750-7587
    DOI 10.1152/japplphysiol.00039.2023
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  2. Article ; Online: Should New Data on Rehabilitation Interventions in Critically Ill Patients Change Clinical Practice? Updated Meta-Analysis of Randomized Controlled Trials.

    Jiroutková, Kateřina / Duška, František / Waldauf, Petr

    Critical care medicine

    2024  

    Abstract: Objectives: We published a meta-analysis in March 2020 to assess the impact of rehabilitation in the ICU on clinical outcomes. Since then, 15 new randomized controlled trials (RCTs) have been published; we updated the meta-analysis to show how the ... ...

    Abstract Objectives: We published a meta-analysis in March 2020 to assess the impact of rehabilitation in the ICU on clinical outcomes. Since then, 15 new randomized controlled trials (RCTs) have been published; we updated the meta-analysis to show how the recent studies have tipped the scale.
    Design: Systematic review and meta-analysis.
    Setting: An update of secondary data analysis of RCTs published between January 1998 and July 2023 performed in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.
    Patients: Critically ill adults.
    Interventions: Cycling exercises or neuromuscular electrical stimulation (NMES) or protocolized physical rehabilitation (PPR) or functional electrical stimulation-assisted cycle ergometry (FESCE) compared with standard of care.
    Measurements and main results: Days on a mechanical ventilator, length of stay in ICU and at the hospital, and mortality. We found 15 RCTs (one on cycling, eight on NMES alone, four on PPR, and two on FESCE) into which 2116 patients were randomized. The updated meta-analysis encompasses a total of 5664 patients. The exercise interventions did not influence mortality (odds ratio, 1.00 [0.87-1.14]; n = 53 RCTs) but reduced the duration of mechanical ventilation (mean difference, -1.76 d [-2.8 to -0.8 d]; n = 46) and length of stay in ICU (-1.16 d [-2.3 to 0.0 d]; n = 45). The effects on the length of mechanical ventilation and ICU stay were only significant for the PPR subgroup by a median of -1.7 days (95% CI, -3.2 to -0.2 d) and -1.9 days (95% CI, -3.5 to -0.2 d), respectively. Notably, newly published trials provided consistent results and reduced the overall heterogeneity of these results.
    Conclusions: None of the rehabilitation intervention strategies being studied influence mortality. Both mechanical ventilation and ICU stay were shortened by PPR, this strengthens the earlier findings as all new RCTs yielded very consistent results. However, no early rehabilitation interventions in passive patients seem to have clinical benefits. Regarding long-term functional outcomes, the results remain inconclusive.
    Language English
    Publishing date 2024-03-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 197890-1
    ISSN 1530-0293 ; 0090-3493
    ISSN (online) 1530-0293
    ISSN 0090-3493
    DOI 10.1097/CCM.0000000000006259
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    Zs.A 1261: Show issues Location:
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  3. Article ; Online: Assessment of Faecal Microbiota Transplant Stability in Deep-Freeze Conditions: A 12-Month Ex Vivo Viability Analysis.

    Soukupova, Hana / Rehorova, Veronika / Cibulkova, Ivana / Duska, Frantisek

    Journal of clinical laboratory analysis

    2024  Volume 38, Issue 7, Page(s) e25023

    Abstract: Background: Faecal microbiota transplantation (FMT) is an established treatment for Clostridioides difficile infection and is under investigation for other conditions. The availability of suitable donors and the logistics of fresh stool preparation ... ...

    Abstract Background: Faecal microbiota transplantation (FMT) is an established treatment for Clostridioides difficile infection and is under investigation for other conditions. The availability of suitable donors and the logistics of fresh stool preparation present challenges, making frozen, biobanked stools an attractive alternative.
    Aims: This study aimed to evaluate the long-term viability of bacterial populations in faecal samples stored at -80°C for up to 12 months, supporting the feasibility of using frozen grafts for FMT.
    Methods: Fifteen faecal samples from nine healthy donors were processed, mixed with cryoprotectants and stored at -80°C. Samples were assessed at baseline and after 3, 6 and 12 months using quantitative culturing methods to determine the concentration of live bacteria.
    Results: Quantitative analysis showed no significant decrease in bacterial viability over the 12-month period for both aerobic and anaerobic cultures (p = 0.09). At all timepoints, the coefficients of variability in colony-forming unit (CFU) counts were greater between samples (102 ± 21% and 100 ± 13% for aerobic and anaerobic cultures, respectively) than the variability between measurements of the same sample (30 ± 22% and 30 ± 19%).
    Conclusions: The study confirmed that faecal microbiota can be preserved with high viability in deep-freeze storage for up to a year, making allogenic FMT from biobanked samples a viable and safer option for patients. However, a multidonor approach may be beneficial to mitigate the risk of viability loss in any single donor sample.
    MeSH term(s) Humans ; Fecal Microbiota Transplantation/methods ; Feces/microbiology ; Microbial Viability ; Freezing ; Cryopreservation/methods ; Male
    Language English
    Publishing date 2024-03-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 645095-7
    ISSN 1098-2825 ; 0887-8013
    ISSN (online) 1098-2825
    ISSN 0887-8013
    DOI 10.1002/jcla.25023
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    Zs.A 2471: Show issues Location:
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  4. Article ; Online: β-hydroxybutyrate exposure restores mitochondrial function in skeletal muscle satellite cells of critically ill patients.

    Genserová, Lucie / Duška, František / Krajčová, Adéla

    Clinical nutrition (Edinburgh, Scotland)

    2024  Volume 43, Issue 6, Page(s) 1250–1260

    Abstract: Background & aim: Dysfunction of skeletal muscle satellite cells might impair muscle regeneration and prolong ICU-acquired weakness, a condition associated with disability and delayed death. This study aimed to elucidate the distinct metabolic effects ... ...

    Abstract Background & aim: Dysfunction of skeletal muscle satellite cells might impair muscle regeneration and prolong ICU-acquired weakness, a condition associated with disability and delayed death. This study aimed to elucidate the distinct metabolic effects of critical illness and β-OH-butyrate on satellite cells isolated from these patients.
    Methods: Satellite cells were extracted from vastus lateralis muscle biopsies of patients with ICU-acquired weakness (n = 10) and control group of healthy volunteers or patients undergoing elective hip replacement surgery (n = 10). The cells were exposed to standard culture media supplemented with β-OH-butyrate to assess its influence on cell proliferation by ELISA, mitochondrial functions by extracellular flux analysis, electron transport chain complexes by high resolution respirometry, and ROS production by confocal microscopy.
    Results: Critical illness led to a decline in maximal respiratory capacity, ATP production and glycolytic capacity and increased ROS production in ICU patients' cells. Notably, the function of complex II was impaired due to critical illness but restored to normal levels upon exposure to β-OH-butyrate. While β-OH-butyrate significantly reduced ROS production in both control and ICU groups, it had no significant impact on global mitochondrial functions.
    Conclusion: Critical illness induces measurable bioenergetic dysfunction of skeletal muscle satellite cells. β-OH-butyrate displayed a potential in rectifying complex II dysfunction caused by critical illness and this warrants further exploration.
    Language English
    Publishing date 2024-04-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 604812-2
    ISSN 1532-1983 ; 0261-5614
    ISSN (online) 1532-1983
    ISSN 0261-5614
    DOI 10.1016/j.clnu.2024.04.009
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  5. Article ; Online: We wish you a smooth takeoff! Launching "Intensive Care Fundamentals": an ESICM educational initiative for newcomers to intensive care unit.

    Duska, Frantisek / Cecconi, Maurizio

    Intensive care medicine

    2022  Volume 48, Issue 12, Page(s) 1778–1780

    MeSH term(s) Humans ; Intensive Care Units ; Critical Care
    Language English
    Publishing date 2022-11-07
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 80387-x
    ISSN 1432-1238 ; 0340-0964 ; 0342-4642 ; 0935-1701
    ISSN (online) 1432-1238
    ISSN 0340-0964 ; 0342-4642 ; 0935-1701
    DOI 10.1007/s00134-022-06906-8
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  6. Article ; Online: Real-Time Effects of Disconnection of the Ventilator on Regional Lung Volumes and Ventilation Distribution.

    Minaříková, Petronela / Borges, João Batista / Duška, František / Zvoníček, Václav

    Respiratory care

    2024  Volume 69, Issue 5, Page(s) 608–612

    Language English
    Publishing date 2024-04-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 603252-7
    ISSN 1943-3654 ; 0098-9142 ; 0020-1324
    ISSN (online) 1943-3654
    ISSN 0098-9142 ; 0020-1324
    DOI 10.4187/respcare.11550
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    Zs.B 2285: Show issues Location:
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  7. Article ; Online: Toward a unified understanding of blood buffer power.

    Krbec, Martin / Duška, František / Elbers, Paul W G

    Journal of applied physiology (Bethesda, Md. : 1985)

    2023  Volume 135, Issue 1, Page(s) 202

    MeSH term(s) Acid-Base Equilibrium ; Hydrogen-Ion Concentration
    Language English
    Publishing date 2023-06-27
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 219139-8
    ISSN 1522-1601 ; 0021-8987 ; 0161-7567 ; 8750-7587
    ISSN (online) 1522-1601
    ISSN 0021-8987 ; 0161-7567 ; 8750-7587
    DOI 10.1152/japplphysiol.00320.2023
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  8. Article ; Online: Perimortem dynamics of blood potassium concentration in patients dying in intensive care unit: A prospective nested cohort study.

    Schmidt, Matouš / Waldauf, Petr / Krbec, Martin / Duška, František

    Journal of forensic sciences

    2022  Volume 67, Issue 4, Page(s) 1550–1556

    Abstract: To enable diagnosis of hyperkalemia from the perimortem blood sample, we aim to describe the natural dynamics of blood potassium [ ... ...

    Abstract To enable diagnosis of hyperkalemia from the perimortem blood sample, we aim to describe the natural dynamics of blood potassium [K
    MeSH term(s) Cohort Studies ; Humans ; Hyperkalemia/complications ; Intensive Care Units ; Ions ; Potassium ; Prospective Studies
    Chemical Substances Ions ; Potassium (RWP5GA015D)
    Language English
    Publishing date 2022-05-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 219216-0
    ISSN 1556-4029 ; 0022-1198
    ISSN (online) 1556-4029
    ISSN 0022-1198
    DOI 10.1111/1556-4029.15054
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  9. Article ; Online: Updated competency-based training in intensive care: next step towards a healthcare union in Europe?

    Póvoa, Pedro / Martin-Loeches, Ignacio / Duska, Frantisek

    Intensive care medicine

    2022  Volume 48, Issue 8, Page(s) 1093–1094

    MeSH term(s) Clinical Competence ; Critical Care ; Delivery of Health Care ; Europe ; Humans
    Language English
    Publishing date 2022-06-17
    Publishing country United States
    Document type Letter
    ZDB-ID 80387-x
    ISSN 1432-1238 ; 0340-0964 ; 0342-4642 ; 0935-1701
    ISSN (online) 1432-1238
    ISSN 0340-0964 ; 0342-4642 ; 0935-1701
    DOI 10.1007/s00134-022-06783-1
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  10. Article ; Online: Amiodarone but not propafenone impairs bioenergetics and autophagy of human myocardial cells.

    Krajčová, Adéla / Němcová, Vlasta / Halačová, Milada / Waldauf, Petr / Balík, Martin / Duška, František

    Toxicology and applied pharmacology

    2023  Volume 477, Page(s) 116676

    Abstract: Cardiac and extra-cardiac side effects of common antiarrhythmic agents might be related to drug-induced mitochondrial dysfunction. Supratherapeutic doses of amiodarone have been shown to impair mitochondria in animal studies, whilst influence of ... ...

    Abstract Cardiac and extra-cardiac side effects of common antiarrhythmic agents might be related to drug-induced mitochondrial dysfunction. Supratherapeutic doses of amiodarone have been shown to impair mitochondria in animal studies, whilst influence of propafenone on cellular bioenergetics is unknown. We aimed to assess effects of protracted exposure to pharmacologically relevant doses of amiodarone and propafenone on cellular bioenergetics and mitochondrial biology of human and mouse cardiomyocytes. In this study, HL-1 mouse atrial cardiomyocytes and primary human cardiomyocytes derived from the ventricles of the adult heart were exposed to 2 and 7 μg/mL of either amiodarone or propafenone. After 24 h, extracellular flux analysis and confocal laser scanning microscopy were used to measure mitochondrial functions. Autophagy was assessed by western blots and live-cell imaging of lysosomes. In human cardiomyocytes, amiodarone significantly reduced mitochondrial membrane potential and ATP production, in association with an inhibition of fatty acid oxidation and impaired complex I- and II-linked respiration in the electron transport chain. Expectedly, this led to increased anaerobic glycolysis. Amiodarone increased the production of reactive oxygen species and autophagy was also markedly affected. In contrast, propafenone-exposed cardiomyocytes did not exert any impairment of cellular bioenergetics. Similar changes after amiodarone treatment were observed during identical experiments performed on HL-1 mouse cardiomyocytes, suggesting a comparable pharmacodynamics of amiodarone among mammalian species. In conclusion, amiodarone but not propafenone in near-therapeutic concentrations causes a pattern of mitochondrial dysfunction with affected autophagy and metabolic switch from oxidative metabolism to anaerobic glycolysis in human cardiomyocytes.
    Language English
    Publishing date 2023-09-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 204477-8
    ISSN 1096-0333 ; 0041-008X
    ISSN (online) 1096-0333
    ISSN 0041-008X
    DOI 10.1016/j.taap.2023.116676
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