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  1. Article: Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells.

    Meng, Guizhi / Duan, Hongjuan / Jia, Jingying / Liu, Baobao / Ma, Yun / Cai, Xiaoyan

    Animal bioscience

    2024  Volume 37, Issue 3, Page(s) 509–521

    Abstract: Objective: It was shown that microRNAs (miRNAs) play an important role in milk protein synthesis. However, the post-transcriptional regulation of casein expression by exogenous miRNA (xeno-miRNAs) in ruminants remains unclear. This study explores the ... ...

    Abstract Objective: It was shown that microRNAs (miRNAs) play an important role in milk protein synthesis. However, the post-transcriptional regulation of casein expression by exogenous miRNA (xeno-miRNAs) in ruminants remains unclear. This study explores the regulatory roles of alfalfa xeno-miR162 on casein synthesis in bovine mammary epithelial cells (bMECs).
    Methods: The effects of alfalfa xenomiR-162 and G protein subunit gamma 11 (GNG11) on proliferation and milk protein metabolism of bMECs were detected by 5-Ethynyl-2'-Deoxyuridine (EdU) staining, flow cytometry, cell counting kit-8 (CCK-8), enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot. Dual-luciferase reporter assay was used to verify the targeting relationship between GNG11 and xenomiR-162.
    Results: Results showed that over-expression of xenomiR-162 inhibited cell proliferation but promoted apoptosis, which also up-regulated the expression of several casein coding genes, including CSN1S1, CSN1S2, and CSN3, while decreasing the expression of CSN2. Furthermore, the targeting relationship between GNG11 and xenomiR-162 was determined, and it was confirmed that GNG11 silencing also inhibited cell proliferation but promoted apoptosis and reduced the expression of casein coding genes and genes related to the mammalian target of rapamycin (mTOR) pathway.
    Conclusion: Alfalfa xenomiR-162 appears to regulate bMECs proliferation and milk protein synthesis via GNG11 in the mTOR pathway, suggesting that this xeno-miRNA could be harnessed to modulate CSN3 expression in dairy cows, and increase κ-casein contents in milk.
    Language English
    Publishing date 2024-01-20
    Publishing country Korea (South)
    Document type Journal Article
    ISSN 2765-0189
    ISSN 2765-0189
    DOI 10.5713/ab.23.0370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Alfalfa xeno-miR159a regulates bovine mammary epithelial cell proliferation and milk protein synthesis by targeting PTPRF.

    Duan, Hongjuan / Li, Shaojin / Li, Chuangwei / Tian, Lan / Ma, Yun / Cai, Xiaoyan

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 9117

    Abstract: Milk protein content is an important index to evaluate the quality and nutrition of milk. Accumulating evidence suggests that microRNAs (miRNAs) play important roles in bovine lactation, but little is known regarding the cross-kingdom regulatory roles of ...

    Abstract Milk protein content is an important index to evaluate the quality and nutrition of milk. Accumulating evidence suggests that microRNAs (miRNAs) play important roles in bovine lactation, but little is known regarding the cross-kingdom regulatory roles of plant-derived exogenous miRNAs (xeno-miRNAs) in milk protein synthesis, particularly the underlying molecular mechanisms. The purpose of this study was to explore the regulatory mechanism of alfalfa-derived xeno-miRNAs on proliferation and milk protein synthesis in bovine mammary epithelial cells (BMECs). Our previous study showed that alfalfa miR159a (mtr-miR159a, xeno-miR159a) was highly expressed in alfalfa, and the abundance of mtr-miR159a was significantly lower in serum and whey from high-protein-milk dairy cows compared with low-protein-milk dairy cows. In this study, mRNA expression was detected by real-time quantitative PCR (qRT-PCR), and casein content was evaluated by enzyme-linked immunosorbent assay (ELISA). Cell proliferation and apoptosis were detected using the cell counting kit 8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU) staining, western blot, and flow cytometry. A dual-luciferase reporter assay was used to determine the regulation of Protein Tyrosine Phosphatase Receptor Type F (PTPRF) by xeno-miR159a. We found that xeno-miR159a overexpression inhibited proliferation of BMEC and promoted cell apoptosis. Besides, xeno-miR159a overexpression decreased β-casein abundance, and increased α-casein and κ-casein abundance in BMECs. Dual-luciferase reporter assay result confirmed that PTPRF is a target gene of xeno-miR159a. These results provide new insights into the mechanism by which alfalfa-derived miRNAs regulate BMECs proliferation and milk protein synthesis.
    MeSH term(s) Female ; Cattle ; Animals ; Milk Proteins/metabolism ; Medicago sativa/genetics ; Medicago sativa/metabolism ; Phosphoric Monoester Hydrolases/metabolism ; Mammary Glands, Animal/metabolism ; Caseins/genetics ; Caseins/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Cell Proliferation ; Luciferases/metabolism ; Epithelial Cells/metabolism
    Chemical Substances Milk Proteins ; Phosphoric Monoester Hydrolases (EC 3.1.3.2) ; Caseins ; MicroRNAs ; Luciferases (EC 1.13.12.-)
    Language English
    Publishing date 2024-04-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-59948-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: High-Grade Ferronickel Concentrates Prepared from Laterite Nickel Ore by a Carbothermal Reduction and Magnetic Separation Method.

    Zhang, Jingzhe / Cao, Chang / Xue, Zhengliang / Li, Faliang / Li, Shaoping / Duan, Hongjuan / Zhang, Haijun

    Materials (Basel, Switzerland)

    2023  Volume 16, Issue 22

    Abstract: Nickel is widely used in industrial processes and plays a crucial role in many applications. However, most of the nickel resource mainly exists as nickel oxide in laterite nickel ore with complex composition, resulting in difficulty in upgrading the ... ...

    Abstract Nickel is widely used in industrial processes and plays a crucial role in many applications. However, most of the nickel resource mainly exists as nickel oxide in laterite nickel ore with complex composition, resulting in difficulty in upgrading the nickel content using physical separation methods. In this study, high-grade ferronickel concentrates were obtained through a carbothermal reduction and magnetic separation using laterite nickel ore and anthracite as raw materials. The effects of different types of additives (CaF
    Language English
    Publishing date 2023-11-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma16227132
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Alfalfa Xeno-miR168b Target

    Jia, Jingying / Duan, Hongjuan / Liu, Baobao / Ma, Yanfen / Ma, Yun / Cai, Xiaoyan

    Metabolites

    2023  Volume 13, Issue 1

    Abstract: It was shown that microRNAs (miRNAs) play an important role in the synthesis of milk fat; thus, this manuscript evaluated whether exogenous miRNA (xeno-miRNAs) from alfalfa could influence the milk fat content in dairy cows. At first, mtr-miR168b was ... ...

    Abstract It was shown that microRNAs (miRNAs) play an important role in the synthesis of milk fat; thus, this manuscript evaluated whether exogenous miRNA (xeno-miRNAs) from alfalfa could influence the milk fat content in dairy cows. At first, mtr-miR168b was screened from dairy cow milk and blood. Then, EdU staining, flow cytometry, Oil Red O staining, qRT-PCR, and WB were applied to explore the effect of xeno-miR168b on the proliferation, apoptosis, and lipid metabolism of bovine mammary epithelial cells (BMECs). Finally, in order to clarify the pathway that regulated the lipid metabolism of BMECs using xeno-miR168b, a double-luciferase reporter assay was used to verify the target gene related to milk fat. These results showed that overexpression of xeno-miR168b inhibited cell proliferation but promoted apoptosis, which also decreased the expression of several lipid metabolism genes, including
    Language English
    Publishing date 2023-01-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662251-8
    ISSN 2218-1989
    ISSN 2218-1989
    DOI 10.3390/metabo13010076
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A robust air superhydrophilic/superoleophobic diatomite porous ceramic for high-performance continuous separation of oil-in-water emulsion.

    Li, Xiaojian / Han, Lei / Huang, Zhong / Li, Zhi / Li, Faliang / Duan, Hongjuan / Huang, Liang / Jia, Quanli / Zhang, Haijun / Zhang, Shaowei

    Chemosphere

    2022  Volume 303, Issue Pt 1, Page(s) 134756

    Abstract: Three-dimensional (3D) porous architecture has attracted considerable attention in remediation of oil/water emulsion. In present work, an air superhydrophilic/superoleophobic diatomite porous ceramic (AS-DC) was prepared, using ... ...

    Abstract Three-dimensional (3D) porous architecture has attracted considerable attention in remediation of oil/water emulsion. In present work, an air superhydrophilic/superoleophobic diatomite porous ceramic (AS-DC) was prepared, using SiO
    MeSH term(s) Ceramics ; Diatomaceous Earth ; Emulsions ; Hydrophobic and Hydrophilic Interactions ; Oils/chemistry ; Porosity ; Silicon Dioxide
    Chemical Substances Emulsions ; Oils ; Diatomaceous Earth (61790-53-2) ; diatomite (68855-54-9) ; Silicon Dioxide (7631-86-9)
    Language English
    Publishing date 2022-05-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2022.134756
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2540: Show issues Location:
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  6. Article: A robust air superhydrophilic/superoleophobic diatomite porous ceramic for high-performance continuous separation of oil-in-water emulsion

    Li, Xiaojian / Han, Lei / Huang, Zhong / Li, Zhi / Li, Faliang / Duan, Hongjuan / Huang, Liang / Jia, Quanli / Zhang, Haijun / Zhang, Shaowei

    Elsevier Ltd Chemosphere. 2022 Sept., v. 303

    2022  

    Abstract: Three-dimensional (3D) porous architecture has attracted considerable attention in remediation of oil/water emulsion. In present work, an air superhydrophilic/superoleophobic diatomite porous ceramic (AS-DC) was prepared, using SiO₂ whiskers modified ... ...

    Abstract Three-dimensional (3D) porous architecture has attracted considerable attention in remediation of oil/water emulsion. In present work, an air superhydrophilic/superoleophobic diatomite porous ceramic (AS-DC) was prepared, using SiO₂ whiskers modified diatomite ceramic as the substrate and FS-50 as the modifier. The interconnected SiO₂ whiskers intertwined on the skeleton of ceramic block forming a 3D network structure, which not only improved the wettability of AS-DC, but also reinforced its mechanical property (about 2.5 MPa of compressive strength). The as-prepared AS-DC with intrinsically superoleophobicity (154°) and superhydrophilicity (0°) exhibited an underwater oil contact angle of 161°, suggesting a multifunctional separation capability. By simply assembling AS-DC with pipes and a pump, it could not only separate the surfactant-stabilized oil-in-water emulsion in a permeation flux as high as 107.8 kg min⁻¹ m⁻² with a selectivity of >95%, but also collect the clean water from the floating oil/water mixture in a flux of 197.4 kg min⁻¹ m⁻² and a selectivity of ∼99%. In addition, the AS-DC was resistant to the salt/acid/alkaline corrosion and temperature fluctuation. The mechanical/chemical firmness of AS-DC renders it tremendous potential as a robust 3D architecture in real application for purification of oil/water mixture.
    Keywords air ; ceramics ; compression strength ; contact angle ; corrosion ; diatomaceous earth ; emulsions ; firmness ; hydrophilicity ; mechanical properties ; oils ; permeability ; remediation ; temperature ; wettability
    Language English
    Dates of publication 2022-09
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2022.134756
    Database NAL-Catalogue (AGRICOLA)

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2540: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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