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Article ; Online: Effect of Hemoporfin-mediated photodynamic therapy in the treatment of facial port-wine stains on intraocular pressure.

Chai, Heng / Duan, Wenjing / Weng, Jiannan / Liu, Dayuan / Ma, Liangjuan

2023 Volume 44, Page(s) 103840

Abstract: Background: Photodynamic therapy (PDT) is a potential treatment for port-wine stains (PWS), but its effects on intraocular pressure (IOP) have not been reported. This study evaluated the efficacy of PDT for facial PWS and analyzed the changes in IOP ... ...

Abstract	Background: Photodynamic therapy (PDT) is a potential treatment for port-wine stains (PWS), but its effects on intraocular pressure (IOP) have not been reported. This study evaluated the efficacy of PDT for facial PWS and analyzed the changes in IOP before and after treatment. Methods: Data from 32 patients with facial PWS who underwent single PDT treatment at our department were collected. The patients were divided into three groups based on the location of the PWS. Group A (15 cases) involved the eyelid of the eye being measured; Group B (10 cases) was located near the eyes but did not involve the measured eyelid; and Group C (7 cases) was situated on the face but not near the eyes. IOP measurements were taken before and after treatment, and the efficacy and changes in IOP were analyzed. Results: The overall efficacy rates of single PDT were 84.37 %, demonstrating superior efficacy for the pink type, age < 6 years, and skin lesions < 10 cm Conclusions: Eyelid involvement in PWS increases the risk of elevated IOP. Hemoporfin-mediated PDT can reduce the IOP in patients with PWS involving the eyelid within a safe range. PDT for facial PWS is considered to be safe and effective.
MeSH term(s)	Humans ; Child ; Photosensitizing Agents/pharmacology ; Photosensitizing Agents/therapeutic use ; Port-Wine Stain/drug therapy ; Port-Wine Stain/pathology ; Photochemotherapy/methods ; Intraocular Pressure ; Glaucoma/drug therapy
Chemical Substances	hematoporphyrin monomethyl ether ; Photosensitizing Agents
Language	English
Publishing date	2023-10-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2149918-4
ISSN	1873-1597 ; 1572-1000
ISSN (online)	1873-1597
ISSN	1572-1000
DOI	10.1016/j.pdpdt.2023.103840
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Article ; Online: Application of metagenomic next-generation sequencing in the diagnosis and treatment of recurrent urinary tract infection in kidney transplant recipients.

Duan, Wenjing / Yang, Yongguang / Zhao, Jingge / Yan, Tianzhong / Tian, Xiangyong

Frontiers in public health

2022 Volume 10, Page(s) 901549

Abstract: Background: Rapid and accurate pathogen diagnosis is an urgent unmet clinical need for recurrent urinary tract infection (RUTI) in kidney transplant recipients (KTRs). Metagenomic next-generation sequencing (mNGS) may offer another strategy for ... ...

Abstract	Background: Rapid and accurate pathogen diagnosis is an urgent unmet clinical need for recurrent urinary tract infection (RUTI) in kidney transplant recipients (KTRs). Metagenomic next-generation sequencing (mNGS) may offer another strategy for diagnosing uropathogens but remains to be studied. Methods: Nineteen KTRs with RUTI were collected in this study. The uropathogens were detected and compared by mNGS and urine culture, respectively. Modifications of the anti-infection strategy were also assessed. Results: Rich and diverse pathogens were revealed by mNGS. mNGS was significantly higher than culture in total positive rate (100.0% vs. 31.6%; Conclusion: mNGS has more remarkable etiological diagnostic performance compared with urine culture for KTRs with RUTI to guide anti-infection strategies and, in turn, protect the graft.
MeSH term(s)	High-Throughput Nucleotide Sequencing ; Humans ; Kidney Transplantation ; Metagenomics ; Sensitivity and Specificity ; Urinary Tract Infections/diagnosis
Language	English
Publishing date	2022-08-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2022.901549
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Article: Phosphorylation of ovalbumin after pulsed electric fields pretreatment: Effects on conformation and immunoglobulin G/immunoglobulin E-binding ability.

Yang, Wenhua / Duan, Wenjing / Li, Qiuhong / Duan, Dengle / Wang, Qin

Frontiers in nutrition

2022 Volume 9, Page(s) 932428

Abstract: Ovalbumin (OVA) is one of major allergens of hen egg white with excellent nutritional and processing properties. Previous research exhibits that pulsed electric field (PEF) treatment could partially unfold OVA. This may contribute to the improvement of ... ...

Abstract	Ovalbumin (OVA) is one of major allergens of hen egg white with excellent nutritional and processing properties. Previous research exhibits that pulsed electric field (PEF) treatment could partially unfold OVA. This may contribute to the improvement of OVA phosphorylation. In this study, the effect of PEF pretreatment combined with phosphorylation on the structure and immunoglobulin (Ig) G/IgE-binding ability of OVA was investigated. The structural changes were measured by circular dichroism (CD), ultraviolet absorption, and fluorescence spectroscopy. The IgG- and IgE-binding abilities were determined by inhibition enzyme-linked immunosorbent assay (ELISA) using rabbit polyclonal antibodies and egg-allergy patients' sera, respectively. The results showed that PEF pretreatment combined with phosphorylation markedly reduced the IgG- and IgE-binding abilities. It was attributed to the changes in secondary and tertiary structure, which was reflected in the increase of ultraviolet (UV) absorbance, α-helix content, and the increase the molecular weight. Moreover, it suggested PEF pretreatment improved the phosphorylation of OVA and enhanced the reduction of IgG/IgE-binding capacity of phosphorylated OVA. Therefore, PEF pretreatment combined with phosphorylation has the potential for developing a method for OVA desensitization.
Language	English
Publishing date	2022-08-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2776676-7
ISSN	2296-861X
ISSN	2296-861X
DOI	10.3389/fnut.2022.932428
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Article ; Online: Analysis of the chloroplast crotonylome of wheat seedling leaves reveals the roles of crotonylated proteins involved in salt-stress responses.

Zhu, Dong / Liu, Junxian / Duan, Wenjing / Sun, Haocheng / Zhang, Liping / Yan, Yueming

Journal of experimental botany

2023 Volume 74, Issue 6, Page(s) 2067–2082

Abstract: Lysine crotonylation (Kcr) is a novel post-translational modification and its function in plant salt-stress responses remains unclear. In this study, we performed the first comprehensive chloroplast crotonylome analysis of wheat seedling leaves to ... ...

Abstract	Lysine crotonylation (Kcr) is a novel post-translational modification and its function in plant salt-stress responses remains unclear. In this study, we performed the first comprehensive chloroplast crotonylome analysis of wheat seedling leaves to examine the potential functions of Kcr proteins in salt-stress responses. In a total of 471 chloroplast proteins, 1290 Kcr sites were identified as significantly regulated by salt stress, and the Kcr proteins were mainly involved in photosynthesis, protein folding, and ATP synthesis. The identified Kcr sites that responded to salt stress were concentrated within KcrK and KcrF motifs, with the conserved KcrF motif being identified in the Kcr proteins of wheat chloroplasts for the first time. Notably, 10 Kcr sites were identified in fructose-1,6-bisphosphate aldolase (TaFBA6), a key chloroplast metabolic enzyme involved in the Calvin-Benson cycle. Site-directed mutagenesis of TaFBA6 showed that the Kcr at K367 is critical in maintaining its enzymatic activity and in conferring salt tolerance in yeast. Further molecular dynamic simulations and analyses of surface electrostatic potential indicated that the Kcr at K367 could improve the structural stability of TaFBA6 by decreasing the distribution of positive charges on the protein surface to resist alkaline environments, thereby promoting both the activity of TaFBA6 and salt tolerance.
MeSH term(s)	Seedlings/metabolism ; Triticum/metabolism ; Proteome/metabolism ; Chloroplasts/metabolism ; Salt Stress ; Plant Leaves/metabolism ; Protein Processing, Post-Translational
Chemical Substances	Proteome
Language	English
Publishing date	2023-01-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2976-2
ISSN	1460-2431 ; 0022-0957
ISSN (online)	1460-2431
ISSN	0022-0957
DOI	10.1093/jxb/erad006
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Article: Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd

Luo, Fei / Zhu, Dong / Sun, Haocheng / Zou, Rong / Duan, Wenjing / Liu, Junxian / Yan, Yueming

Frontiers in plant science

2023 Volume 14, Page(s) 1103241

Abstract: Cadmium, one of the toxic heavy metals, robustly impact crop growth and development and food safety. In this study, the mechanisms of wheat ( ...

Abstract	Cadmium, one of the toxic heavy metals, robustly impact crop growth and development and food safety. In this study, the mechanisms of wheat (
Language	English
Publishing date	2023-02-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2023.1103241
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Book ; Article ; Online: Rent sharing in China

Duan, Wenjing / Martins, Pedro S.

Magnitude, heterogeneity and drivers

2020

Abstract: Do firms in China share rents with their workers? We address this question by examining firm-level panel data covering virtually all manufacturing firms over the period 2000-2007, representing an average of 52 million workers per year. We find evidence ... ...

Abstract	Do firms in China share rents with their workers? We address this question by examining firm-level panel data covering virtually all manufacturing firms over the period 2000-2007, representing an average of 52 million workers per year. We find evidence of rent sharing (RS), with wage-profit elasticies of between 4% and 6%. These results are based on multiple instrumental variables, including firm-specific international trade shocks. We also present a number of complementary findings to understand better the nature of RS in the country: it involves an element of risk sharing, as wages also decrease when profits fall; RS is lower in regions with more latent competition from rural workers; higher minimum wages tend to reduce RS; and, while employer labour market power reduces wages, it increases RS. Overall, despite its importance, RS in China is smaller and more symmetric than in developed economies, which re ects the weaker bargaining power of its workers and the different nature of its labour market institutions.
Keywords	ddc:330 ; J31 ; J41 ; J50 ; Wages ; Bargaining ; Monopsony
Subject code	338
Language	English
Publisher	Essen: Global Labor Organization (GLO)
Publishing country	de
Document type	Book ; Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Dynamic proteome changes of wheat developing grains in response to water deficit and high-nitrogen fertilizer conditions

Duan, Wenjing / Zhu, Gengrui / Zhu, Dong / Yan, Yueming

Plant physiology and biochemistry. 2020 Nov., v. 156

2020

Abstract: This study investigated grain proteomic profiles in response to water deficit, high nitrogen (N) fertilizer, and their combined treatments in elite Chinese bread wheat cultivar Jingdong 17, using a two-dimensional difference gel electrophoresis (2D-DIGE)- ...

Abstract	This study investigated grain proteomic profiles in response to water deficit, high nitrogen (N) fertilizer, and their combined treatments in elite Chinese bread wheat cultivar Jingdong 17, using a two-dimensional difference gel electrophoresis (2D-DIGE)-based approach. Water deficit negatively affected the main agronomic traits of wheat and grain yield, while high-N fertilizer had the opposite effects. The application of a high-N fertilizer under water deficit conditions moderately improved kernel development and grain yield. 2D-DIGE led to the identification of 124 differentially accumulated protein (DAP) spots during five different grain developmental stages, corresponding to 97 unique proteins. The more significant changes of DAPs occurred at 10–20 days after flowering. DAPs were involved in carbohydrate metabolism, protein turnover, protein folding, cell cycle control, stress response, nitrogen metabolism, photosynthesis, and energy metabolism. In particular, water deficit caused a significant downregulation of proteins involved in starch biosynthesis, whereas high-N fertilizer led to a significant upregulation of proteins involved in nitrogen metabolism, carbohydrate metabolism, and starch biosynthesis. The combined treatment resulted in a moderate upregulation of DAPs related to carbohydrate metabolism, starch biosynthesis, and nitrogen metabolism. Our results indicated that high-N fertilization could alleviate yield loss caused by water deficit by promoting the accumulation of proteins involved in nitrogen and carbohydrate metabolism.
Keywords	biosynthesis ; cell cycle ; cultivars ; gel electrophoresis ; grain yield ; nitrogen ; nitrogen metabolism ; photosynthesis ; protein metabolism ; proteome ; proteomics ; seed development ; starch ; stress response ; wheat
Language	English
Dates of publication	2020-11
Size	p. 471-483.
Publishing place	Elsevier Masson SAS
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2020.08.022
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Article ; Online: Dynamic proteome changes of wheat developing grains in response to water deficit and high-nitrogen fertilizer conditions.

Duan, Wenjing / Zhu, Gengrui / Zhu, Dong / Yan, Yueming

Plant physiology and biochemistry : PPB

2020 Volume 156, Page(s) 471–483

Abstract	This study investigated grain proteomic profiles in response to water deficit, high nitrogen (N) fertilizer, and their combined treatments in elite Chinese bread wheat cultivar Jingdong 17, using a two-dimensional difference gel electrophoresis (2D-DIGE)-based approach. Water deficit negatively affected the main agronomic traits of wheat and grain yield, while high-N fertilizer had the opposite effects. The application of a high-N fertilizer under water deficit conditions moderately improved kernel development and grain yield. 2D-DIGE led to the identification of 124 differentially accumulated protein (DAP) spots during five different grain developmental stages, corresponding to 97 unique proteins. The more significant changes of DAPs occurred at 10-20 days after flowering. DAPs were involved in carbohydrate metabolism, protein turnover, protein folding, cell cycle control, stress response, nitrogen metabolism, photosynthesis, and energy metabolism. In particular, water deficit caused a significant downregulation of proteins involved in starch biosynthesis, whereas high-N fertilizer led to a significant upregulation of proteins involved in nitrogen metabolism, carbohydrate metabolism, and starch biosynthesis. The combined treatment resulted in a moderate upregulation of DAPs related to carbohydrate metabolism, starch biosynthesis, and nitrogen metabolism. Our results indicated that high-N fertilization could alleviate yield loss caused by water deficit by promoting the accumulation of proteins involved in nitrogen and carbohydrate metabolism.
MeSH term(s)	Carbohydrate Metabolism ; Droughts ; Fertilizers/analysis ; Nitrogen/metabolism ; Plant Proteins ; Proteome ; Triticum/physiology ; Water
Chemical Substances	Fertilizers ; Plant Proteins ; Proteome ; Water (059QF0KO0R) ; Nitrogen (N762921K75)
Language	English
Publishing date	2020-08-13
Publishing country	France
Document type	Journal Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2020.08.022
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Article: Septic Shock After Kidney Transplant: A Rare Bloodstream

Tian, Xiangyong / Jing, Nan / Duan, Wenjing / Wu, Xiaoqiang / Zhang, Chan / Wang, Shanmei / Yan, Tianzhong

Infection and drug resistance

2022 Volume 15, Page(s) 3841–3845

Abstract: Background: Ralstonia mannitolilytica: Case presentation: We present a case of septic shock after renal transplant in a 41-year-old male, which was finally proven to be caused by : Conclusion: This study highlights ... ...

Abstract	Background: Ralstonia mannitolilytica Case presentation: We present a case of septic shock after renal transplant in a 41-year-old male, which was finally proven to be caused by Conclusion: This study highlights that
Language	English
Publishing date	2022-07-19
Publishing country	New Zealand
Document type	Case Reports
ZDB-ID	2494856-1
ISSN	1178-6973
ISSN	1178-6973
DOI	10.2147/IDR.S370170
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Article ; Online: 2D-DIGE based proteome analysis of wheat-Thinopyrum intermedium 7XL/7DS translocation line under drought stress.

Lu, Fengkun / Duan, Wenjing / Cui, Yue / Zhang, Junwei / Zhu, Dong / Zhang, Ming / Yan, Yueming

BMC genomics

2022 Volume 23, Issue 1, Page(s) 369

Abstract: Background: Drought stress is the most limiting factor for plant growth and crop production worldwide. As a major cereal crop, wheat is susceptible to drought. Thus, discovering and utilizing drought-tolerant gene resources from related species are ... ...

Abstract	Background: Drought stress is the most limiting factor for plant growth and crop production worldwide. As a major cereal crop, wheat is susceptible to drought. Thus, discovering and utilizing drought-tolerant gene resources from related species are highly important for improving wheat drought resistance. In this study, the drought tolerance of wheat Zhongmai 8601-Thinopyrum intermedium 7XL/7DS translocation line YW642 was estimated under drought stress, and then two-dimensional difference gel electrophoresis (2D-DIGE) based proteome analysis of the developing grains was performed to uncover the drought-resistant proteins. Results: The results showed that 7XL/7DS translocation possessed a better drought-tolerance compared to Zhongmai 8601. 2D-DIGE identified 146 differential accumulation protein (DAP) spots corresponding to 113 unique proteins during five grain developmental stages of YW642 under drought stress. Among them, 55 DAP spots corresponding to 48 unique proteins displayed an upregulated expression, which were mainly involved in stress/defense, energy metabolism, starch metabolism, protein metabolism/folding and transport. The cis-acting element analysis revealed that abundant stress-related elements were present in the promoter regions of the drought-responsive protein genes, which could play important roles in drought defense. RNA-seq and RT-qPCR analyses revealed that some regulated DAP genes also showed a high expression level in response to drought stress. Conclusions: Our results indicated that Wheat-Th. intermedium 7XL/7DS translocation line carried abundant drought-resistant proteins that had potential application values for wheat drought tolerance improvement.
MeSH term(s)	Droughts ; Edible Grain/metabolism ; Plant Proteins/metabolism ; Proteome/metabolism ; Triticum/metabolism ; Two-Dimensional Difference Gel Electrophoresis
Chemical Substances	Plant Proteins ; Proteome
Language	English
Publishing date	2022-05-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2041499-7
ISSN	1471-2164 ; 1471-2164
ISSN (online)	1471-2164
ISSN	1471-2164
DOI	10.1186/s12864-022-08599-1
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