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Article ; Online: The Combined Effect of Nanobubble-IR783-HPPH-Affibody Complex and Laser on HER2-Positive Breast Cancer.

Cai, Wenbin / Lv, Wei / Meng, Li / Duan, Yunyou / Zhang, Li

2023 Volume 18, Page(s) 339–351

Abstract: Introduction: Nanobubble is an innovative ultrasound contrast agent that triggers the development of targeted imaging of HER2-positive breast cancer by combining with HER2 affibody and IR783. HPPH is a second-generation photosensitiser that is effective ...

Abstract	Introduction: Nanobubble is an innovative ultrasound contrast agent that triggers the development of targeted imaging of HER2-positive breast cancer by combining with HER2 affibody and IR783. HPPH is a second-generation photosensitiser that is effective in treating tumours. Hence, the nanobubble-IR783-HPPH-affibody (NIHA) complex demonstrates considerable potential in the treatment of HER2-positive breast cancer. Methods: We fabricated the NIHA complex via an advanced thin-film hydration method and detected its characteristics such as particle size, morphology, stability, and cytotoxicity. Moreover, the effect of NIHA complex with laser on HER2-positive breast cancer was confirmed via in vitro and in vivo experiments. Results: The NIHA complex was spheroid, stable and exhibited no cytotoxicity; moreover, its particle size was 524.8 ± 53.3 nm (n = 5). In combination with laser treatment, NIHA complex reduced the cell viability and tumour volume, induced apoptosis of HER2-positive breast cancer cells, and prolonged survival of nude mice. Conclusion: The newly prepared NIHA complex with laser treatment has the potential on treating HER2-positive breast cancer.
MeSH term(s)	Animals ; Mice ; Mice, Nude ; Cell Line, Tumor ; Ultrasonography ; Lasers ; Receptor, ErbB-2 ; Neoplasms
Chemical Substances	2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (149402-51-7) ; Receptor, ErbB-2 (EC 2.7.10.1)
Language	English
Publishing date	2023-01-19
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2364941-0
ISSN	1178-2013 ; 1176-9114
ISSN (online)	1178-2013
ISSN	1176-9114
DOI	10.2147/IJN.S387409
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Article ; Online: Clinical Value of TCCD for Evaluating the Prognosis of Patients with Severe Traumatic Brain Injury After Large Decompressive Craniectomy: A Retrospective Study.

Liang, Yuan / Duan, Yunyou / Xing, Changyang / Jin, Jinglan / Yan, Lingjuan / Liu, Xi / Wang, Jia

Advances in therapy

2022 Volume 39, Issue 10, Page(s) 4556–4567

Abstract: Introduction: It is challenging to assess the prognosis of patients with severe traumatic brain injury (sTBI) after large decompressive craniectomy (DC). The aim of this study was to evaluate the clinical value of transcranial color-coded duplex ... ...

Abstract	Introduction: It is challenging to assess the prognosis of patients with severe traumatic brain injury (sTBI) after large decompressive craniectomy (DC). The aim of this study was to evaluate the clinical value of transcranial color-coded duplex sonography (TCCD) for assessing the prognosis of sTBI patients 6 months after large DC. Methods: This was a retrospective observational study that consecutively enrolled 84 patients with sTBI who were followed up for prognosis until 6 months after large DC. The primary endpoint was the Glasgow Outcome Score (GOS). According to the GOS, patients were divided into an unfavorable prognosis group (GOS 1-3, n = 47) and a favorable prognosis group (GOS 4-5, n = 37). Results: Significant between-group differences were found in age and hemodynamic parameters (systolic peak blood flow velocity, end-diastolic blood flow velocity, mean blood flow velocity, pulsatility index and resistance index) of the middle cerebral artery detected by TCCD (P < 0.05 for all). Subsequently, ridge regression was used to build a prognostic model for patients with large DC. Based on the cerebral hemodynamic parameters measured by TCCD and age, the mean (± standard deviation) area under the curve of the prognostic model in patients with sTBI after large DC was 0.76 ± 0.22. The sensitivity and specificity were 82.08% and 74.17%, respectively. Conclusions: The cerebral hemodynamic parameters detected by TCCD, combined with age, may be used to predict the outcomes of patients with sTBI at 6 months after large DC. As a noninvasive method, TCCD has the potential to assess the prognosis of these patients. Trial registration: ChiCTR: ChiCTR1800019758. Registered 27 November 2018-retrospectively registered ( http://www.chictr.org.cn/index.aspx ).
MeSH term(s)	Brain Injuries, Traumatic/diagnostic imaging ; Brain Injuries, Traumatic/surgery ; Decompressive Craniectomy/methods ; Humans ; Infant ; Prognosis ; Retrospective Studies ; Sensitivity and Specificity ; Treatment Outcome
Language	English
Publishing date	2022-08-07
Publishing country	United States
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	632651-1
ISSN	1865-8652 ; 0741-238X
ISSN (online)	1865-8652
ISSN	0741-238X
DOI	10.1007/s12325-022-02251-w
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Zs.A 2101: Show issues

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Article ; Online: AHNAK-modified microbubbles for the intracranial delivery of triptolide: In-vitro and in-vivo investigations.

Feng, Yang / An, Rui / Zhang, Yajun / Chen, Mingjun / Wang, Li / Duan, Yunyou / Xing, Changyang

International journal of pharmaceutics

2022 Volume 629, Page(s) 122351

Abstract: Autophagic dysfunction related cascade events might induce the accumulation of α-synuclein (αSyn) in Parkinson's disease (PD). Triptolide ( ... ...

Abstract	Autophagic dysfunction related cascade events might induce the accumulation of α-synuclein (αSyn) in Parkinson's disease (PD). Triptolide (T
MeSH term(s)	Animals ; Mice ; Microbubbles ; Phenanthrenes ; Diterpenes ; Epoxy Compounds ; Parkinson Disease ; Membrane Proteins ; Neoplasm Proteins
Chemical Substances	triptolide (19ALD1S53J) ; Phenanthrenes ; Diterpenes ; Epoxy Compounds ; Ahnak protein, mouse ; Membrane Proteins ; Neoplasm Proteins
Language	English
Publishing date	2022-10-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2022.122351
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Article ; Online: Preliminary study of carotid variables under ultrasound analysis as predictors for the risk of coronary arterial atherosclerosis.

Cai, Wen-Bin / Wang, Yi / Wang, Jia / Guo, Wan-Gang / Duan, Yun-You / Zhang, Li

Echocardiography (Mount Kisco, N.Y.)

2022 Volume 39, Issue 8, Page(s) 1054–1063

Abstract: Background: Carotid atherosclerosis by ultrasound scanning can be considered as an ideal window to reflect systemic artery atherosclerosis, which has aroused wide concern for predicting the severity of coronary artery atherosclerosis clinically. ... ...

Abstract	Background: Carotid atherosclerosis by ultrasound scanning can be considered as an ideal window to reflect systemic artery atherosclerosis, which has aroused wide concern for predicting the severity of coronary artery atherosclerosis clinically. Ultrasound radio frequency (RF) data technology has enabled us to evaluate the carotid structure and elastic function precisely, for predicting the severity of coronary artery atherosclerosis. Methods: Patients with suspected coronary artery disease (CAD) underwent coronary angiography and were assigned to four groups according to whether atherosclerotic plaque was found or not and it caused stenosis. Carotid artery intima-media thickness (IMT) and arterial stiffness were investigated by quality intima-media thickness (QIMT) and quality arterial stiffness (QAS) techniques during ultrasound scanning. Univariable and multivariable modeling were used to investigate correlations of carotid parameters to coronary artery atherosclerosis. Receive operating characteristic (ROC) curves were used to evaluate diagnostic performance of these ultrasound variables. Results: Carotid IMT and stiffness variables pulse wave velocity (PWV), α, β and compliance coefficient (CC) were statistically different between every two-group's comparisons. IMT correlated with stiffness variables significantly with r = 0.70, 0.77, 0.63, and -0.39, respectively. All variables correlated with the severity of coronary atherosclerosis with the odd ratio (OR) of 1.73, 1.67, 1.19, 1.23, and 0.56 accordingly as IMT, PWV, α, β and CC were concerned. The AUC of IMT, PWV, α, β and CC were 0.9257, 0.8910, 0.8016, 0.9383, 0.8581 with correctly classified rate of 88.16%, 83.77%, 78.07%, 86.84%, and 81.58%, respectively. Conclusions: Carotid artery IMT and stiffness variable PWV, α, β and CC presented favorable predicting and differentiating values for patients with coronary atherosclerosis of different severity.
MeSH term(s)	Atherosclerosis ; Carotid Arteries ; Carotid Intima-Media Thickness ; Coronary Artery Disease ; Humans ; Pulse Wave Analysis
Language	English
Publishing date	2022-07-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	843645-9
ISSN	1540-8175 ; 0742-2822
ISSN (online)	1540-8175
ISSN	0742-2822
DOI	10.1111/echo.15404
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Zs.A 2170: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Supplementation of Clostridium butyricum Alleviates Vascular Inflammation in Diabetic Mice.

Zhou, Tian / Qiu, Shuo / Zhang, Liang / Li, Yangni / Zhang, Jing / Shen, Donghua / Zhao, Ping / Yuan, Lijun / Zhao, Lianbi / Duan, Yunyou / Xing, Changyang

Diabetes & metabolism journal

2024

Abstract: Background: Gut microbiota is closely related to the occurrence and development of diabetes and affects the prognosis of diabetic complications, and the underlying mechanisms are only partially understood. We aimed to explore the possible link between ... ...

Abstract	Background: Gut microbiota is closely related to the occurrence and development of diabetes and affects the prognosis of diabetic complications, and the underlying mechanisms are only partially understood. We aimed to explore the possible link between the gut microbiota and vascular inflammation of diabetic mice. Methods: The db/db diabetic and wild-type (WT) mice were used in this study. We profiled gut microbiota and examined the and vascular function in both db/db group and WT group. Gut microbiota was analyzed by 16s rRNA sequencing. Vascular function was examined by ultrasonographic hemodynamics and histological staining. Clostridium butyricum (CB) was orally administered to diabetic mice by intragastric gavage every 2 days for 2 consecutive months. Reactive oxygen species (ROS) and expression of nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by fluorescence microscopy. The mRNA expression of inflammatory cytokines was tested by quantitative polymerase chain reaction. Results: Compared with WT mice, CB abundance was significantly decreased in the gut of db/db mice, together with compromised vascular function and activated inflammation in the arterial tissue. Meanwhile, ROS in the vascular tissue of db/db mice was also significantly increased. Oral administration of CB restored the protective microbiota, and protected the vascular function in the db/db mice via activating the Nrf2/HO-1 pathway. Conclusion: This study identified the potential link between decreased CB abundance in gut microbiota and vascular inflammation in diabetes. Therapeutic delivery of CB by gut transplantation alleviates the vascular lesions of diabetes mellitus by activating the Nrf2/HO-1 pathway.
Language	English
Publishing date	2024-02-02
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2602402-0
ISSN	2233-6087 ; 2233-6087
ISSN (online)	2233-6087
ISSN	2233-6087
DOI	10.4093/dmj.2023.0109
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Article ; Online: Targeted delivery of MerTK protein via cell membrane engineered nanoparticle enhances efferocytosis and attenuates atherosclerosis in diabetic ApoE

Qiu, Shuo / Liu, Jiahan / Chen, Jianmei / Li, Yangni / Bu, Te / Li, Zhelong / Zhang, Liang / Sun, Wenqi / Zhou, Tian / Hu, Wei / Yang, Guodong / Yuan, Lijun / Duan, Yunyou / Xing, Changyang

Journal of nanobiotechnology

2024 Volume 22, Issue 1, Page(s) 178

Abstract: Background: Clearance of apoptotic cells by efferocytosis is crucial for prevention of atherosclerosis progress, and impaired efferocytosis contributes to the aggravated atherosclerosis.: Results: In this study, we found that diabetic ApoE: ... ...

Abstract	Background: Clearance of apoptotic cells by efferocytosis is crucial for prevention of atherosclerosis progress, and impaired efferocytosis contributes to the aggravated atherosclerosis. Results: In this study, we found that diabetic ApoE Conclusions: Our study indicates that MerTK decrease in macrophages contributes to the aggravated atherosclerosis in diabetic ApoE
MeSH term(s)	Humans ; Animals ; Mice ; Efferocytosis ; HEK293 Cells ; Cell Membrane ; Atherosclerosis ; Protein-Tyrosine Kinases ; Apolipoproteins E/genetics ; Magnetic Iron Oxide Nanoparticles ; Diabetes Mellitus
Chemical Substances	Protein-Tyrosine Kinases (EC 2.7.10.1) ; Apolipoproteins E
Language	English
Publishing date	2024-04-13
Publishing country	England
Document type	Journal Article
ZDB-ID	2100022-0
ISSN	1477-3155 ; 1477-3155
ISSN (online)	1477-3155
ISSN	1477-3155
DOI	10.1186/s12951-024-02463-y
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Article ; Online: Intraoperative contrast-enhanced ultrasound for cerebral glioma resection and the relationship between microvascular perfusion and microvessel density.

Wang, Jia / Yang, Yilin / Liu, Xi / Duan, Yunyou

Clinical neurology and neurosurgery

2019 Volume 186, Page(s) 105512

Abstract: We analyzed the relationship between quantitative CEUS parameters and microvessel density (MVD) of different pathologic grades of cerebral gliomas. ICEUS was performed in 49 patients with cerebral gliomas. The enhancement characteristics of cerebral ... ...

Abstract	We analyzed the relationship between quantitative CEUS parameters and microvessel density (MVD) of different pathologic grades of cerebral gliomas. ICEUS was performed in 49 patients with cerebral gliomas. The enhancement characteristics of cerebral gliomas were observed before and after tumor resection. The number of microvessels was counted by immunostaining with anti-CD34. Differences in these quantitative parameters in cerebral gliomas were compared and subjected to a correlation analysis with MVD. The assessment of iCEUS parameters and tumor MVD showed that cerebral gliomas of different pathological grades had different characteristics. The time-to-peak (Tmax) was significantly shorter, the peak intensity (PI) and MVD were significantly higher in high-grade cerebral gliomas than in low-grade cerebral gliomas (p < 0.05). According to the immunostaining, PI was positively (r = 0.637) correlated with MVD and Tmax was negatively (r = -0.845) correlated with MVD. ICEUS could provid dynamic and continuous real-time imaging and quantitative data analysis of different pathological grades of cerebral gliomas, the quantitiative CEUS parameters were closely related to the MVD, and be helpful in understanding the cerebral gliomas grade and refining surgical strategy.
MeSH term(s)	Adolescent ; Adult ; Aged ; Brain Neoplasms/diagnostic imaging ; Brain Neoplasms/surgery ; Female ; Glioma/diagnostic imaging ; Glioma/surgery ; Humans ; Intraoperative Neurophysiological Monitoring/methods ; Male ; Microvessels/diagnostic imaging ; Middle Aged ; Ultrasonography, Interventional/methods ; Young Adult
Language	English
Publishing date	2019-09-03
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	193107-6
ISSN	1872-6968 ; 0303-8467
ISSN (online)	1872-6968
ISSN	0303-8467
DOI	10.1016/j.clineuro.2019.105512
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Article ; Online: Early Assessment of Atherosclerotic Lesions and Vulnerable Plaques in vivo by Targeting Apoptotic Macrophages with AV Nanobubbles.

Ma, Xiaoju / Wang, Jia / Li, Zhelong / Zhou, Xueying / Liang, Xiao / Wang, Junyan / Duan, Yunyou / Zhao, Ping

International journal of nanomedicine

2022 Volume 17, Page(s) 4933–4946

Abstract: Background: The early detection of atherosclerotic lesions is particularly important for risk prediction of acute cardiovascular events. Macrophages apoptosis was significantly associated with the degree of AS lesions and especially contributed to ... ...

Abstract	Background: The early detection of atherosclerotic lesions is particularly important for risk prediction of acute cardiovascular events. Macrophages apoptosis was significantly associated with the degree of AS lesions and especially contributed to plaque vulnerability. In this research, we mainly sought to explore the feasibility of a home-made AV-nanobubbles (NB Methods: NB Results: The prepared NB Conclusion: NB
MeSH term(s)	Animals ; Mice ; Avidin ; Biotin ; Plaque, Atherosclerotic/diagnostic imaging ; Plaque, Atherosclerotic/pathology ; Atherosclerosis/diagnostic imaging ; Atherosclerosis/pathology ; Apolipoproteins E/genetics ; Macrophages/pathology ; Molecular Probes
Chemical Substances	Avidin (1405-69-2) ; Biotin (6SO6U10H04) ; Apolipoproteins E ; Molecular Probes
Language	English
Publishing date	2022-10-19
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2364941-0
ISSN	1178-2013 ; 1176-9114
ISSN (online)	1178-2013
ISSN	1176-9114
DOI	10.2147/IJN.S382738
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Article ; Online: The severity of portal hypertension by a non-invasive assessment: acoustic structure quantification analysis of liver parenchyma.

Cai, Wen-Bin / Yin, Ji-Kai / Li, Qiao-Ying / Yang, Yi-Lin / Duan, Yun-You / Zhang, Li

BMC medical imaging

2022 Volume 22, Issue 1, Page(s) 85

Abstract: Background: Acoustic structure quantification (ASQ) has been applied to evaluate liver histologic changes by analyzing the speckle pattern seen on B-mode ultrasound. We aimed to assess the severity of portal hypertension (PHT) through hepatic ... ...

Abstract	Background: Acoustic structure quantification (ASQ) has been applied to evaluate liver histologic changes by analyzing the speckle pattern seen on B-mode ultrasound. We aimed to assess the severity of portal hypertension (PHT) through hepatic ultrasonography. Methods: Sixty patients diagnosed with PHT and underwent surgical treatment with portosystemic shunts were enrolled. Portal pressure (PP) was measured intraoperatively. Patients were divided into subgroups according to the severity of gastroesophageal varices and Child-Pugh class. Three difference ratio (C Results: In the patients, the ASQ parameters peak C Conclusions: ASQ analysis of ultrasonographic images may have a role in the evaluation of the severity of PHT by detecting liver histologic changes in the speckle pattern caused by cirrhosis.
MeSH term(s)	Acoustics ; Esophageal and Gastric Varices/diagnostic imaging ; Humans ; Hypertension, Portal/diagnostic imaging ; Liver/diagnostic imaging ; Liver Cirrhosis/diagnostic imaging ; Varicose Veins
Language	English
Publishing date	2022-05-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2061975-3
ISSN	1471-2342 ; 1471-2342
ISSN (online)	1471-2342
ISSN	1471-2342
DOI	10.1186/s12880-022-00817-2
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Article ; Online: Colon specific delivery of miR-155 inhibitor alleviates estrogen deficiency related phenotype via microbiota remodeling.

Zhao, Lianbi / Zhou, Tian / Chen, Jianmei / Cai, Wenbin / Shi, Ruijing / Duan, Yunyou / Yuan, Lijun / Xing, Changyang

Drug delivery

2022 Volume 29, Issue 1, Page(s) 2610–2620

Abstract: Compelling data have indicated menopause-associated increase in cardiovascular disease in women, while the underlying mechanisms remain largely unknown. It is established that changes of intestinal microbiota affect cardiovascular function in the context ...

Abstract	Compelling data have indicated menopause-associated increase in cardiovascular disease in women, while the underlying mechanisms remain largely unknown. It is established that changes of intestinal microbiota affect cardiovascular function in the context of metabolic syndrome. We here aimed to explore the possible link between host intestinal function, microbiota, and cardiac function in the ovariectomy (OVX) mouse model. Mice were ovariectomized to induce estrogen-related metabolic syndrome and cardiovascular defect. Microbiota was analyzed by 16s rRNA sequencing. miRNA and mRNA candidates expression were tested by qPCR. Cardiac function was examined by echocardiography. Colon specific delivery of miRNA candidates was achieved by oral gavage of Eudragit S100 functionalized microspheres. In comparison with the sham-operated group, OVX mice showed compromised cardiac function, together with activated inflammation in the visceral adipose tissue and heart.
MeSH term(s)	Animals ; Colon/metabolism ; Estrogens ; Female ; Gastrointestinal Microbiome ; Humans ; Metabolic Syndrome ; Mice ; MicroRNAs/genetics ; Phenotype ; RNA, Ribosomal, 16S
Chemical Substances	Estrogens ; MIRN155 microRNA, human ; MicroRNAs ; Mirn155 microRNA, mouse ; RNA, Ribosomal, 16S
Language	English
Publishing date	2022-08-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1213261-5
ISSN	1521-0464 ; 1071-7544
ISSN (online)	1521-0464
ISSN	1071-7544
DOI	10.1080/10717544.2022.2108163
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