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  1. Article ; Online: Functional characterisation of a soybean galactinol synthase gene under various stress conditions

    Duc Ha Chu / Buffel Melanie / Tien Dung Le

    Vietnam Journal of Science, Technology and Engineering, Vol 60, Iss

    2018  Volume 3

    Abstract: Galactinol synthase (GolS) has been known to play a key role in raffinose biosynthesis by catalysing the formation of galactinol. The GolS gene family has been recently identified in various plant species. Among them, many individual GolS genes have been ...

    Abstract Galactinol synthase (GolS) has been known to play a key role in raffinose biosynthesis by catalysing the formation of galactinol. The GolS gene family has been recently identified in various plant species. Among them, many individual GolS genes have been reported to function in plant stress tolerance. In this study, we reported the construction of transgenic Arabidopsis overexpressing a soybean GolS gene, GolS2. There were no significant differences in the phenotypes of the transgenic and control plants during normal physiological conditions. We evaluated the performance of the transgenic plants under various stress conditions in relation to that of the control plants. The result evidenced that the overexpression of GmGolS2 gene in Arabidopsis improved the plant’s tolerance to salt stress but did not protect the plants against heavy metals and paraquat. Our study suggested that soybean GolS genes could be a potential candidate for genetic engineering to improve abiotic stress tolerance of plants.
    Keywords Arabidopsis thaliana ; galactinol synthase ; overexpression ; phenotypic analysis ; stress tolerance ; Science ; Q
    Subject code 580
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher Vietnam Ministry of Science and Technology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Genome-wide identification and annotation of the Nuclear-factor YA gene family in cassava (Manihot esculenta Crantz)

    Duc Ha Chu / Thi Thuy Tam Do / Xuan Dac Le / Thi Ly Thu Pham

    Vietnam Journal of Science, Technology and Engineering, Vol 59, Iss

    2017  Volume 3

    Abstract: Nuclear factor Y (NF-Y) is a transcription factor which plays an important role in the regulation of various developmental processes and stress responses in plants. By using various bioinformatics tools, the identification and analyses of the NF-YA ... ...

    Abstract Nuclear factor Y (NF-Y) is a transcription factor which plays an important role in the regulation of various developmental processes and stress responses in plants. By using various bioinformatics tools, the identification and analyses of the NF-YA subunit of cassava (Manihot esculenta Crantz) have been attempted in this study. A total of 12 members of the NF-YA gene family were identified in the cassava genome. They were located on the 18 cassava chromosomes with different frequencies. Several initial structural analyses of the NF-YA family were also performed. Among them, the typical gene organization of the MeNF-YA gene family contained 5 exons/4 introns. Interestingly, the conserved region of NF-YA was characterized by the interaction of NF-YB/C domain and the DNA binding domain. This study provided information on NF-Y in plants.
    Keywords cassava ; gene ; in silico ; NF-YA ; transcription factor ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2017-09-01T00:00:00Z
    Publisher Vietnam Ministry of Science and Technology
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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