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  1. Book ; Online ; E-Book: Mitochondrial dysfunction in neurodegenerative disorders

    Reeve, Amy Katherine / Simcox, Eve M. / Duchen, Michael R. / Turnbull, Doug M.

    2016  

    Author's details Amy K. Reeve, Eve M. Simcox, Michael R. Duchen, Doug M. Turnbull editors
    Language English
    Size 1 Online-Ressource (x, 380 Seiten), Illustrationen
    Edition Second edition
    Publisher Springer
    Publishing place Cham
    Publishing country Switzerland
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT019057223
    ISBN 978-3-319-28637-2 ; 9783319286358 ; 3-319-28637-4 ; 3319286358
    DOI 10.1007/978-3-319-28637-2
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Analysis of Organization and Activity of Mitochondrial Respiratory Chain Complexes in Primary Fibroblasts Using Blue Native PAGE.

    Singh, Kritarth / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 339–348

    Abstract: Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a well-established technique for the isolation and separation of mitochondrial membrane protein complexes in a native conformation with high resolution. In combination with histochemical ... ...

    Abstract Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a well-established technique for the isolation and separation of mitochondrial membrane protein complexes in a native conformation with high resolution. In combination with histochemical staining methods, BN-PAGE has been successfully used as clinical diagnostic tool for the detection of oxidative phosphorylation (OXPHOS) defects from small tissue biopsies from patients with primary mitochondrial disease. However, its application to patient-derived primary fibroblasts is difficult due to limited proliferation and high background staining. Here, we describe a rapid and convenient method to analyze the organization and activity of OXPHOS complexes from cultured skin fibroblasts.
    MeSH term(s) Electron Transport ; Electrophoresis, Polyacrylamide Gel ; Fibroblasts ; Humans ; Mitochondrial Membranes ; Native Polyacrylamide Gel Electrophoresis/methods
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_25
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Investigating Mitochondrial Ca

    Bhosale, Gauri / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 325–332

    Abstract: Mitochondrial ... ...

    Abstract Mitochondrial Ca
    MeSH term(s) Calcium/metabolism ; Calcium Signaling ; Cytosol/metabolism ; Fluorescent Dyes/metabolism ; Mitochondria/metabolism
    Chemical Substances Fluorescent Dyes ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging.

    Valdebenito, Gabriel Esteban / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 319–324

    Abstract: The mitochondrial membrane potential ( ... ...

    Abstract The mitochondrial membrane potential (ΔΨ
    MeSH term(s) Cells, Cultured ; Membrane Potential, Mitochondrial ; Mitochondria/metabolism ; Optical Imaging ; Time-Lapse Imaging
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX.

    Chung, Chih-Yao / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 333–337

    Abstract: Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we ... ...

    Abstract Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment.
    MeSH term(s) Ethidium/analogs & derivatives ; Oxidative Stress ; Phenanthridines ; Reactive Oxygen Species/analysis ; Superoxides/analysis
    Chemical Substances 5-(6'-triphenylphosphoniumhexyl)-5,6-dihydro-6-phenyl-3,8-phenanthridinediammine ; Phenanthridines ; Reactive Oxygen Species ; dihydroethidium (104821-25-2) ; Superoxides (11062-77-4) ; Ethidium (EN464416SI)
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: The mitochondrial ATP synthase as an ATP consumer-a surprising therapeutic target.

    Valdebenito, Gabriel E / Chacko, Anitta R / Duchen, Michael R

    The EMBO journal

    2023  Volume 42, Issue 10, Page(s) e114141

    Abstract: The mitochondrial ... ...

    Abstract The mitochondrial F
    MeSH term(s) Mitochondrial Proton-Translocating ATPases/metabolism ; Adenosine Triphosphate ; Protons ; Mitochondria/metabolism
    Chemical Substances Mitochondrial Proton-Translocating ATPases (EC 3.6.3.-) ; Adenosine Triphosphate (8L70Q75FXE) ; Protons
    Language English
    Publishing date 2023-04-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 586044-1
    ISSN 1460-2075 ; 0261-4189
    ISSN (online) 1460-2075
    ISSN 0261-4189
    DOI 10.15252/embj.2023114141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1808: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 100: Show issues

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  7. Article ; Online: NAD(P)H binding configurations revealed by time-resolved fluorescence and two-photon absorption.

    Blacker, Thomas S / Duchen, Michael R / Bain, Angus J

    Biophysical journal

    2023  Volume 122, Issue 7, Page(s) 1240–1253

    Abstract: NADH and NADPH play key roles in the regulation of metabolism. Their endogenous fluorescence is sensitive to enzyme binding, allowing changes in cellular metabolic state to be determined using fluorescence lifetime imaging microscopy (FLIM). However, to ... ...

    Abstract NADH and NADPH play key roles in the regulation of metabolism. Their endogenous fluorescence is sensitive to enzyme binding, allowing changes in cellular metabolic state to be determined using fluorescence lifetime imaging microscopy (FLIM). However, to fully uncover the underlying biochemistry, the relationships between their fluorescence and binding dynamics require greater understanding. Here we accomplish this through time- and polarization-resolved fluorescence and polarized two-photon absorption measurements. Two lifetimes result from binding of both NADH to lactate dehydrogenase and NADPH to isocitrate dehydrogenase. The composite fluorescence anisotropy indicates the shorter (1.3-1.6 ns) decay component to be accompanied by local motion of the nicotinamide ring, pointing to attachment solely via the adenine moiety. For the longer lifetime (3.2-4.4 ns), the nicotinamide conformational freedom is found to be fully restricted. As full and partial nicotinamide binding are recognized steps in dehydrogenase catalysis, our results unify photophysical, structural, and functional aspects of NADH and NADPH binding and clarify the biochemical processes that underlie their contrasting intracellular lifetimes.
    MeSH term(s) NAD/chemistry ; NAD/metabolism ; NADP ; Fluorescence ; Niacinamide ; Catalysis
    Chemical Substances NAD (0U46U6E8UK) ; NADP (53-59-8) ; Niacinamide (25X51I8RD4)
    Language English
    Publishing date 2023-02-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218078-9
    ISSN 1542-0086 ; 0006-3495
    ISSN (online) 1542-0086
    ISSN 0006-3495
    DOI 10.1016/j.bpj.2023.02.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 235: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 2: Show issues

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  8. Article ; Online: Assessing the Redox Status of Mitochondria Through the NADH/FAD

    Chi, Haoyu / Bhosale, Gauri / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 313–318

    Abstract: This section aims to describe the measurement of NADH and ... ...

    Abstract This section aims to describe the measurement of NADH and FAD
    MeSH term(s) Flavin-Adenine Dinucleotide/metabolism ; Microscopy, Fluorescence ; Mitochondria/metabolism ; NAD/metabolism ; Oxidation-Reduction
    Chemical Substances NAD (0U46U6E8UK) ; Flavin-Adenine Dinucleotide (146-14-5)
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Investigating the Mitochondrial Permeability Transition Pore in Disease Phenotypes and Drug Screening.

    Bhosale, Gauri / Duchen, Michael R

    Current protocols in pharmacology

    2019  Volume 85, Issue 1, Page(s) e59

    Abstract: Mitochondria act as 'sinks' for ... ...

    Abstract Mitochondria act as 'sinks' for Ca
    MeSH term(s) Animals ; Calcium/metabolism ; Cryopreservation ; Drug Evaluation, Preclinical/methods ; Immunoblotting ; Membrane Potential, Mitochondrial ; Mice ; Mitochondria, Liver ; Mitochondrial Membrane Transport Proteins/metabolism ; Mitochondrial Permeability Transition Pore ; Oxygen Consumption ; Phenotype
    Chemical Substances Mitochondrial Membrane Transport Proteins ; Mitochondrial Permeability Transition Pore ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2019-05-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2179074-7
    ISSN 1934-8290 ; 1934-8282
    ISSN (online) 1934-8290
    ISSN 1934-8282
    DOI 10.1002/cpph.59
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single- or Two-Photon Confocal Microscopy.

    Davidson, Sean M / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1782, Page(s) 171–186

    Abstract: The concentration of calcium ions in the mitochondria has been shown to affect its function, modulating respiratory activity at low levels and causing lethal damage at high concentrations. The rhodamine series of dyes can be used to measure mitochondrial ...

    Abstract The concentration of calcium ions in the mitochondria has been shown to affect its function, modulating respiratory activity at low levels and causing lethal damage at high concentrations. The rhodamine series of dyes can be used to measure mitochondrial calcium concentration, but the reliability of measurements depends upon correct partitioning of the dye within to the mitochondria. Methods are described to aid verification and quantification of the mitochondrial calcium concentration using single- or two-photon confocal microscopy. The method of linear unmixing to separate fluorescent signals based on either differing excitation or emission spectra is outlined and for the purposes of illustration is applied to the separation of rhod-2 signals originating from the dye within the mitochondria and nucleoli.
    MeSH term(s) Animals ; Calcium/analysis ; Calcium/metabolism ; Cell Line ; Fluorescent Dyes/chemistry ; Heterocyclic Compounds, 3-Ring/chemistry ; Intravital Microscopy/instrumentation ; Intravital Microscopy/methods ; Mice ; Microscopy, Confocal/instrumentation ; Microscopy, Confocal/methods ; Microscopy, Fluorescence, Multiphoton/instrumentation ; Microscopy, Fluorescence, Multiphoton/methods ; Mitochondria/metabolism ; Photons ; Reproducibility of Results
    Chemical Substances Fluorescent Dyes ; Heterocyclic Compounds, 3-Ring ; rhod-2 (10I3106D4A) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2018-05-31
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7831-1_10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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