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  1. Article: Sluchai virusnykh gepatitov sochetannoĭ étiologii.

    Dudik, T V / Sharafanova, T I / Il'ichenko, L Iu

    Rossiiskii gastroenterologicheskii zhurnal : ezhekvartalnyi nauchno-prakticheskii zhurnal

    2001  , Issue 1, Page(s) 81–86

    Abstract: 6 cases of viral hepatitis A in the patients with chronic liver diseases are described. Study of this problem is necessary for the improvement of epidemiologic control, well-timed diagnosis and treatment of these diseases. The possibility of parenteral ... ...

    Title translation Cases of viral hepatitis of a combined etiology.
    Abstract 6 cases of viral hepatitis A in the patients with chronic liver diseases are described. Study of this problem is necessary for the improvement of epidemiologic control, well-timed diagnosis and treatment of these diseases. The possibility of parenteral virus of hepatitis A transmission is discussed.
    MeSH term(s) Adult ; Female ; Hepatitis C/diagnosis ; Hepatitis C/virology ; Humans ; Male
    Language Russian
    Publishing date 2001
    Publishing country Russia (Federation)
    Document type Case Reports ; English Abstract ; Journal Article
    ISSN 1560-408X
    ISSN 1560-408X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Eradikatsiia Helicobacter pylori u bol'nykh iazvennoĭ bolezn'iu dvenadtsatiperstnoĭ kishki posle kratkosrochnogo kursa lecheniia azitromitsinom s amoksitsillinom.

    Reshetniak, V I / Dudik, T V / Solov'eva, N A / Zhukhovitskiĭ, V G / Il'chenko, A A / Kaprel'iants, A S

    Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic

    2002  Volume 47, Issue 2, Page(s) 16–19

    Abstract: The efficacy of short-term treatment with azithromycin in 17 patients with acute doudenal ulcer associated with H. pylori was evaluated. Bioptats of the gastric mucosa taken at the beginning and after one month of treatment were investigated for H. ... ...

    Title translation Eradication of Helicobacter pylori in patients with duodenal ulcer following the short course of treatment with azitromycin and amoxycillin.
    Abstract The efficacy of short-term treatment with azithromycin in 17 patients with acute doudenal ulcer associated with H. pylori was evaluated. Bioptats of the gastric mucosa taken at the beginning and after one month of treatment were investigated for H. pylori presence by histological, bacteriological methods, by urease test and by PCR. All the patients with positive H. pylori test were treated with Gastrozol (omeperazole, ICN Pharmaceutical) 40 mg per day for 1 week followed by 20 mg per day for 3 weeks, Sumamed (azithromycin, peira) 0.5 g once daily for 3 days and amoxycillin 0.5 mg four times a day for 10 days. Bioptats analysis before treatment revealed H. pylori in 100% for PCR methods, in 94.1% for urease test, in 88.2% for histological test. After the treatment H. pylori was revealed in 12.5% for urease and histological test, in 18.8% for bacteriological test and in 25% for PCR test. Thus the treatment efficacy was 75%. Side effects for short-term azithromycin therapy were shown in 5.9% cases.
    MeSH term(s) Adult ; Aged ; Amoxicillin/therapeutic use ; Anti-Bacterial Agents/therapeutic use ; Azithromycin/therapeutic use ; Duodenal Ulcer/drug therapy ; Duodenal Ulcer/microbiology ; Female ; Helicobacter pylori/drug effects ; Helicobacter pylori/isolation & purification ; Humans ; Male ; Middle Aged ; Time Factors
    Chemical Substances Anti-Bacterial Agents ; Amoxicillin (804826J2HU) ; Azithromycin (83905-01-5)
    Language Russian
    Publishing date 2002
    Publishing country Russia (Federation)
    Document type Clinical Trial ; English Abstract ; Journal Article
    ZDB-ID 269110-3
    ISSN 0235-2990
    ISSN 0235-2990
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 64: Show issues Location:
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  3. Article: Metody vyiavleniia Helicobacter pylori.

    Dudik, T V / Solov'eva, N A / Zhukhovitskiĭ, V G / Kirillov, M Iu / Kaprel'iants, A S / Reshetniak, V I

    Rossiiskii gastroenterologicheskii zhurnal : ezhekvartalnyi nauchno-prakticheskii zhurnal

    2001  , Issue 2, Page(s) 77–89

    Abstract: There is the HP diagnostic methods review. The classification of main methods is presented. Diagnostic significance and defects of all these methods were demonstrated. There are own data of HP and its strains revealing in the patients with duodenal ... ...

    Title translation Methods of Helicobacter pylori detection.
    Abstract There is the HP diagnostic methods review. The classification of main methods is presented. Diagnostic significance and defects of all these methods were demonstrated. There are own data of HP and its strains revealing in the patients with duodenal peptic ulcer.
    MeSH term(s) Duodenal Ulcer/diagnosis ; Duodenal Ulcer/microbiology ; Electrophoresis ; Flagellin/genetics ; Helicobacter Infections/enzymology ; Helicobacter Infections/genetics ; Helicobacter Infections/microbiology ; Helicobacter pylori/genetics ; Helicobacter pylori/isolation & purification ; Humans ; Polymorphism, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; Urease/metabolism
    Chemical Substances Flagellin (12777-81-0) ; flaA protein, bacteria (133606-66-3) ; Urease (EC 3.5.1.5)
    Language Russian
    Publishing date 2001
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article ; Review
    ISSN 1560-408X
    ISSN 1560-408X
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  4. Article: Diagnostic methods for detecting forms and strains of Helicobacter pylori and evaluation of its eradication.

    Loginov, A S / Reshetnyak, V I / Dudik, T V / Vostroknutova, G N / Il'chenko, A A / Kaprel'yants, A S

    Bulletin of experimental biology and medicine

    2001  Volume 132, Issue 2, Page(s) 802–806

    Abstract: Diagnostic methods for detecting forms and strains of Helicobacter pylori isolated from biopsy specimens of gastric mucosa in 28 patients with duodenal ulcers and evaluation of its eradication were compared. Biopsy specimens from all patients were tested ...

    Abstract Diagnostic methods for detecting forms and strains of Helicobacter pylori isolated from biopsy specimens of gastric mucosa in 28 patients with duodenal ulcers and evaluation of its eradication were compared. Biopsy specimens from all patients were tested for the presence of H. pylori by the urease test, histological method, and PCR with species-specific primers before and after treatment. H. pylori infection was detected in all patients before treatment, the mean titer of serum IgG being 36.7+/-16.6 U/ml. Biopsy specimens positive for H. pylori in PCR were subjected to restriction analysis of specific PCR-amplified genes or their fragments. The fingerprint analysis gave electrophoregrams of restriction products amplified fragment of flaA gene of H. pylori in 7 patients. Differences in restriction maps indicate the presence of 5 H. pylori strains in the studied samples.
    MeSH term(s) Adolescent ; Adult ; Aged ; Antibodies, Bacterial/blood ; Bismuth/therapeutic use ; DNA Fingerprinting ; Duodenal Ulcer/diagnosis ; Duodenal Ulcer/drug therapy ; Duodenal Ulcer/microbiology ; Duodenal Ulcer/pathology ; Female ; Flagellin/genetics ; Gastric Mucosa/microbiology ; Gastric Mucosa/pathology ; Helicobacter Infections/diagnosis ; Helicobacter Infections/drug therapy ; Helicobacter Infections/pathology ; Helicobacter pylori/genetics ; Helicobacter pylori/immunology ; Helicobacter pylori/isolation & purification ; Humans ; Immunoglobulin G/blood ; Male ; Middle Aged ; Omeprazole/therapeutic use ; Polymerase Chain Reaction ; Ranitidine/analogs & derivatives ; Ranitidine/therapeutic use ; Recurrence ; Restriction Mapping ; Species Specificity ; Time Factors ; Urease/blood
    Chemical Substances Antibodies, Bacterial ; Immunoglobulin G ; Flagellin (12777-81-0) ; flaA protein, bacteria (133606-66-3) ; ranitidine bismuth citrate (7AJ51I17KG) ; Ranitidine (884KT10YB7) ; Urease (EC 3.5.1.5) ; Omeprazole (KG60484QX9) ; Bismuth (U015TT5I8H)
    Language English
    Publishing date 2001-08
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ZDB-ID 390407-6
    ISSN 1573-8221 ; 0007-4888 ; 0365-9615
    ISSN (online) 1573-8221
    ISSN 0007-4888 ; 0365-9615
    DOI 10.1023/a:1013006717904
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    Zs.A 56: Show issues Location:
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  5. Article: Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases.

    Telkov, M V / Demina, G R / Voloshin, S A / Salina, E G / Dudik, T V / Stekhanova, T N / Mukamolova, G V / Kazaryan, K A / Goncharenko, A V / Young, M / Kaprelyants, A S

    Biochemistry. Biokhimiia

    2006  Volume 71, Issue 4, Page(s) 414–422

    Abstract: The secreted Micrococcus luteus protein, Rpf, is required for successful resuscitation of dormant "non-culturable" M. luteus cells and for growth stimulation in poor media. The biochemical mechanism of Rpf action remained unknown. Theoretical predictions ...

    Abstract The secreted Micrococcus luteus protein, Rpf, is required for successful resuscitation of dormant "non-culturable" M. luteus cells and for growth stimulation in poor media. The biochemical mechanism of Rpf action remained unknown. Theoretical predictions of Rpf domain architecture and organization, together with a recent NMR analysis of the protein structure, indicate that the conserved Rpf domain has a lysozyme-like fold. In the present study, we found that both the secreted native protein and the recombinant protein lyse crude preparations of M. luteus cell walls. They also hydrolyze 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside, a synthetic substrate for peptidoglycan muramidases, with optimum activity at pH 6. The Rpf protein also has weak proteolytic activity against N-CBZ-Gly-Gly-Arg-beta-naphthylamide, a substrate for trypsin-like enzymes. Rpf activity towards 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside was reduced when the glutamate residue at position 54, invariant for all Rpf family proteins and presumably involved in catalysis, was altered. The same amino acid substitution resulted in impaired resuscitation activity of Rpf. The data indicate that Rpf is a peptidoglycan-hydrolyzing enzyme, and strongly suggest that this specific activity is responsible for its growth promotion and resuscitation activity. A possible mechanism of Rpf-mediated resuscitation is discussed.
    MeSH term(s) Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Culture Media ; Cytokines/chemistry ; Cytokines/genetics ; Cytokines/metabolism ; Micrococcus luteus/cytology ; Micrococcus luteus/enzymology ; Micrococcus luteus/metabolism ; Mutagenesis, Site-Directed ; N-Acetylmuramoyl-L-alanine Amidase/chemistry ; N-Acetylmuramoyl-L-alanine Amidase/genetics ; N-Acetylmuramoyl-L-alanine Amidase/metabolism ; Peptide Hydrolases/chemistry ; Peptide Hydrolases/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Time Factors
    Chemical Substances Bacterial Proteins ; Culture Media ; Cytokines ; Recombinant Proteins ; resuscitation-promoting factor, bacteria ; Peptide Hydrolases (EC 3.4.-) ; N-Acetylmuramoyl-L-alanine Amidase (EC 3.5.1.28)
    Language English
    Publishing date 2006-03-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1109-5
    ISSN 1608-3040 ; 0006-2979 ; 0320-9717
    ISSN (online) 1608-3040
    ISSN 0006-2979 ; 0320-9717
    DOI 10.1134/s0006297906040092
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  6. Article: O vozmozhnosti prebyvaniia Helicobacter pylori v pokoiashchemsia sostoianii v slizistoĭ obolochke zheludka u bol'nykh iazvennoĭ bolezn'iu posle lecheniia.

    Loginov, A S / Reshetniak, V I / Mukamolova, G V / Fedukova, N G / Il'chenko, A A / Dudik, T V / Kaprel'iants, A S

    Terapevticheskii arkhiv

    1999  Volume 71, Issue 2, Page(s) 13–16

    Abstract: Aim: To develop experimental approaches to detection of inactive forms of Helicobacter pylori (HP) in patients treated for gastroduodenal ulcer, to try the role of such HP forms in emergence of ulcer recurrences.: Materials and methods: Examination ... ...

    Title translation The possibility of Helicobacter pylori being present in the resting state in the gastric mucosa of peptic acid patients after treatment.
    Abstract Aim: To develop experimental approaches to detection of inactive forms of Helicobacter pylori (HP) in patients treated for gastroduodenal ulcer, to try the role of such HP forms in emergence of ulcer recurrences.
    Materials and methods: Examination for HP was made using a fast CLO test, polymerase chain reaction (PCR), histologically. A simple quantitative spectrophotometric test for urease activity in biopsies of gastric mucosa is proposed.
    Results: Antibacterial and other treatments led to a significant decline in urease activity dependent on the amount of the agents in the biopsies. PCR found HP in 48% of patients after the treatment. In these cases HP may be in dormancy. This state is not safe because of possible HP activation eventuating in ulcer recurrence.
    Conclusion: PCR can be used for prediction of ulcer recurrence as it is sensitive to HP when wide-spread urease test gives negative results.
    MeSH term(s) Biopsy ; DNA, Bacterial/genetics ; Duodenal Ulcer/drug therapy ; Duodenal Ulcer/microbiology ; Duodenal Ulcer/pathology ; Female ; Follow-Up Studies ; Gastric Mucosa/enzymology ; Gastric Mucosa/microbiology ; Gastric Mucosa/pathology ; Helicobacter Infections/drug therapy ; Helicobacter Infections/microbiology ; Helicobacter Infections/pathology ; Helicobacter pylori/enzymology ; Helicobacter pylori/genetics ; Helicobacter pylori/isolation & purification ; Humans ; Hydrogen-Ion Concentration ; Male ; Polymerase Chain Reaction ; Recurrence ; Stomach Ulcer/drug therapy ; Stomach Ulcer/microbiology ; Stomach Ulcer/pathology ; Urease/analysis
    Chemical Substances DNA, Bacterial ; Urease (EC 3.5.1.5)
    Language Russian
    Publishing date 1999
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article
    ZDB-ID 40718-5
    ISSN 2309-5342 ; 0040-3660
    ISSN (online) 2309-5342
    ISSN 0040-3660
    Database MEDical Literature Analysis and Retrieval System OnLINE

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