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Article ; Online: Beyond dopamine: Novel strategies for schizophrenia treatment.

Dudzik, Paulina / Lustyk, Klaudia / Pytka, Karolina

2024

Abstract: Despite extensive research efforts aimed at discovering novel antipsychotic compounds, a satisfactory pharmacological strategy for schizophrenia treatment remains elusive. All the currently available drugs act by modulating dopaminergic neurotransmission, ...

Abstract	Despite extensive research efforts aimed at discovering novel antipsychotic compounds, a satisfactory pharmacological strategy for schizophrenia treatment remains elusive. All the currently available drugs act by modulating dopaminergic neurotransmission, leading to insufficient management of the negative and cognitive symptoms of the disorder. Due to these challenges, several attempts have been made to design agents with innovative, non-dopaminergic mechanisms of action. Consequently, a number of promising compounds are currently progressing through phases 2 and 3 of clinical trials. This review aims to examine the rationale behind the most promising of these strategies while simultaneously providing a comprehensive survey of study results. We describe the versatility behind the cholinergic neurotransmission modulation through the activation of M
Language	English
Publishing date	2024-04-23
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	603210-2
ISSN	1098-1128 ; 0198-6325
ISSN (online)	1098-1128
ISSN	0198-6325
DOI	10.1002/med.22042
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Article: Molecular biology techniques in real life - case study introducing medical students to QF-PCR technique used in medical diagnostics.

Kocemba-Pilarczyk, Kinga A / Bentke-Imiolek, Anna / Dudzik, Paulina

Folia medica Cracoviensia

2023 Volume 63, Issue 2, Page(s) 135–148

Abstract: QF-PCR is a widely used molecular biology method. To name just a few of its uses, it is considered to be useful in paternity tests, identification tests or prenatal diagnostics. Therefore, there is a good chance that medical faculty students would come ... ...

Abstract	QF-PCR is a widely used molecular biology method. To name just a few of its uses, it is considered to be useful in paternity tests, identification tests or prenatal diagnostics. Therefore, there is a good chance that medical faculty students would come into contact with this technology - directly or indirectly - during their professional work. The following article proposes a teaching classes scenario conducted in the problem-based learning manner, which aims to familiarize students with the QF-PCR technique. In addition, other modern methods of molecular genetics are among topics that students can learn during the problem-based learning modules. The classes are divided into three parts. In the first part, students learn about the possible usage of QF-PCR in paternity tests. The second part focuses on learning about the advantages and limitations of QF-PCR in prenatal diagnosis. Learning activities in the last part are designed to show the limitations of the diagnostic properties of the method - students analyze the case study, in which QF-PCR must be replaced by other modern methods of molecular genetics. By analyzing three independent stories, students learn about usage, advantages and limitations of QF-PCR, and additionally gain knowledge in basic, pre-clinical and clinical sciences. This course is designated as an elective course for final year medical students who have completed either: a basic genetics course, a molecular genetics course, a biochemistry course or a molecular biology course. The focus of the classes is to draw students' attention to the possible application and rapid development of molecular biology techniques, which is the base for modern therapeutic and diagnostic strategies.
MeSH term(s)	Pregnancy ; Female ; Humans ; Students, Medical ; Problem-Based Learning/methods ; Learning ; Polymerase Chain Reaction/methods ; Molecular Biology
Language	English
Publishing date	2023-10-30
Publishing country	Poland
Document type	Journal Article
ZDB-ID	412415-7
ISSN	0015-5616
ISSN	0015-5616
DOI	10.24425/fmc.2023.145918
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Article ; Online: In vitro culture Muller cell model to study the role of inverted internal limiting membrane flap technique in macular hole closure.

Morawski, K / Kocemba-Pilarczyk, K / Zarzycka, M / Dudzik, P / Trojan, S E / Laidler, P

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society

2021 Volume 72, Issue 3

Abstract: Inverted internal limiting membrane (ILM) flap may constitute a scaffold for Muller cells whose migration and proliferation on its surface begin the process of macular hole closure. The goal of the study was to establish an in vitro model of the ... ...

Abstract	Inverted internal limiting membrane (ILM) flap may constitute a scaffold for Muller cells whose migration and proliferation on its surface begin the process of macular hole closure. The goal of the study was to establish an in vitro model of the interaction between ILM and the Muller cells. Vitrectomy with inverted ILM flap was performed in 23 patients due to a full-thickness macular hole (FTMH). After dissection of the inverted flap, the area of ILM peeling was extended and material was collected for cell culture experiments. Muller cells cultured on adherent cell plates showed significantly better growth than on suspension plates. Our results reveal that the presence of the ILM can overcome the growth inhibitory effect of the non-adhesive surface. Moreover, the ILM appears to be the optimal growth surface under normoxia conditions mimicking the microenvironment after vitrectomy and hypoxia which is natural state for Muller cells. The closure rate of FTMH was 100%. Our study revealed that in non-adhesive culture conditions patient derived ILM constitutes an optimal growth surface for Muller cells. We have demonstrated that the ILM effectively stimulates attachment, proliferation, and survival of Müller cells in conditions of normoxia which is the case after vitrectomy. The results strongly advocate for the use of inverted ILM flap method in macular hole closure surgeries.
MeSH term(s)	Basement Membrane ; Ependymoglial Cells ; Epiretinal Membrane ; Humans ; Retinal Perforations/surgery ; Retrospective Studies ; Tomography, Optical Coherence ; Visual Acuity
Language	English
Publishing date	2021-11-19
Publishing country	Poland
Document type	Journal Article
ZDB-ID	1125221-2
ISSN	1899-1505 ; 0867-5910 ; 0044-6033
ISSN (online)	1899-1505
ISSN	0867-5910 ; 0044-6033
DOI	10.26402/jpp.2021.3.08
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Article ; Online: Teaching "Biochemistry with Elements of Chemistry" in the COVID-19 pandemic.

Dudzik, Paulina / Bentke-Imiolek, Anna / Kocemba-Pilarczyk, Kinga A / Ostrowska, Barbara

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology

2023 Volume 51, Issue 3, Page(s) 316–324

Abstract: The purpose of the presented article is to evaluate the students' perception of the online teaching educational model as a part of the "Biochemistry with Elements of Chemistry" course conducted during the COVID-19 pandemic at the Faculty of Medicine of ... ...

Abstract	The purpose of the presented article is to evaluate the students' perception of the online teaching educational model as a part of the "Biochemistry with Elements of Chemistry" course conducted during the COVID-19 pandemic at the Faculty of Medicine of the Jagiellonian University Medical College. The first part of the article reflects upon the pandemic impact on the transition from the in-person (standard format) to a complete remote learning format. The next part is based on the responses of the students to a questionnaire and presents an analysis of the students' preferences and perceptions regarding synchronous and asynchronous teaching methods. Students answered questions about the advantages and disadvantages of distance learning programs. They indicated the most suitable learning mode in terms of gaining knowledge and enhancing their motivation to learn. They listed factors that facilitated remote learning as well as those which made it difficult and posed a challenge to adjust to the new system, in all types of classes, that is, lectures, seminars, and laboratories. The last part of this paper presents the results of the students' performance in the pandemic-enforced system and compares them with the results from the previous class of students from the past year, when teaching was conducted mainly in the standard format. The conclusions from the given analysis may enable beneficial changes for the "Biochemistry with Elements of Chemistry" course for remote teaching in the future. It may also provide valuable insights for such types of courses conducted at other Universities and promote deliberations to continuously improve learning outcomes.
MeSH term(s)	Humans ; Pandemics ; COVID-19/epidemiology ; Students ; Learning ; Faculty
Language	English
Publishing date	2023-01-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2039717-3
ISSN	1539-3429 ; 1470-8175
ISSN (online)	1539-3429
ISSN	1470-8175
DOI	10.1002/bmb.21712
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Article ; Online: Does the absence of SARS-CoV-2 specific genes always exclude the infection? How to interpret RT-PCR results?-The scenario of interactive online workshop.

Kocemba-Pilarczyk, Kinga A / Bentke-Imiolek, Anna / Dudzik, Paulina

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology

2021 Volume 49, Issue 4, Page(s) 658–663

Abstract: The aim of this online workshop is to familiarize biomedical faculties students with the principle of RT-PCR method. The following assumption is made, students participating in the workshop: are already familiar with the principle of PCR reaction, can ... ...

Abstract	The aim of this online workshop is to familiarize biomedical faculties students with the principle of RT-PCR method. The following assumption is made, students participating in the workshop: are already familiar with the principle of PCR reaction, can distinguish PCR from RT-PCR, know the basic possibilities of using the above techniques. During the online workshop participants are supposed to learn the interpretation of PCR and RT-PCR results and to understand the crucial importance of controlling the reaction conditions. The workshop involves active students' learning, critical analysis of the data, group discussion, brainstorming method, involvement of e-tools such as pool everywhere or e-learning platforms, as well as interpreting the real-life example results that allows putting the topic in the proper future work-related tasks. The final part of the workshop focuses on the analysis of the RT-PCR results performed in order to confirm or exclude the presence of the SARS-CoV-2 genome in potentially infected individuals. The students are expected to see the practical/work-related part of the knowledge gained during the workshop.
MeSH term(s)	COVID-19/diagnosis ; COVID-19/genetics ; COVID-19 Nucleic Acid Testing ; Education ; Education, Distance ; Humans ; Molecular Biology/education ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics
Language	English
Publishing date	2021-03-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2039717-3
ISSN	1539-3429 ; 1470-8175
ISSN (online)	1539-3429
ISSN	1470-8175
DOI	10.1002/bmb.21506
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Article ; Online: Expression of Alternative Splice Variants of 6-Phosphofructo-2-kinase/Fructose-2,6-bisphosphatase-4 in Normoxic and Hypoxic Melanoma Cells.

Trojan, Sonia E / Dudzik, Paulina / Totoń-Żurańska, Justyna / Laidler, Piotr / Kocemba-Pilarczyk, Kinga A

International journal of molecular sciences

2021 Volume 22, Issue 16

Abstract: Cancer-specific isoenzyme of phosphofructokinase II ( ...

Abstract	Cancer-specific isoenzyme of phosphofructokinase II (
MeSH term(s)	Alternative Splicing ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Gene Expression Regulation, Neoplastic ; Glycolysis ; Humans ; Hypoxia/physiopathology ; Melanoma/genetics ; Melanoma/metabolism ; Melanoma/pathology ; Oxygen/metabolism ; Phosphofructokinase-2/genetics ; Tumor Cells, Cultured
Chemical Substances	Biomarkers, Tumor ; PFKFB4 protein, human ; Phosphofructokinase-2 (EC 2.7.1.105) ; Oxygen (S88TT14065)
Language	English
Publishing date	2021-08-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22168848
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Article: Influence of metformin on HIF-1 pathway in multiple myeloma.

Kocemba-Pilarczyk, Kinga A / Trojan, Sonia / Ostrowska, Barbara / Lasota, Małgorzata / Dudzik, Paulina / Kusior, Dorota / Kot, Marta

Pharmacological reports : PR

2020 Volume 72, Issue 5, Page(s) 1407–1417

Abstract: Background: Multiple myeloma (MM) is defined as plasma cells malignancy, developing in the bone marrow. At the beginning of the disease, the malignant plasma cells are dependent on bone marrow microenvironment, providing growth and survival factors. ... ...

Abstract	Background: Multiple myeloma (MM) is defined as plasma cells malignancy, developing in the bone marrow. At the beginning of the disease, the malignant plasma cells are dependent on bone marrow microenvironment, providing growth and survival factors. Importantly, the recent studies pointed hypoxia as an important factor promoting progression of MM. In particular, hypoxia-triggered HIF-1 signaling was shown to promote chemoresistance, angiogenesis, invasiveness and induction of immature phenotype, suggesting that strategies targeting HIF-1 may contribute to improvement of anti-myeloma therapies. Methods: The Western Blot and RT-PCR techniques were applied to analyze the influence of metformin on HIF-1 pathway in MM cells. To evaluate the effect of metformin on the growth of MM cell lines in normoxic and hypoxic conditions the MTT assay was used. The apoptosis induction in metformin treated hypoxic and normoxic cells was verified by Annexin V/PI staining followed by FACS analysis. Results: Our results showed, for the first time, that metformin inhibits HIF-1 signaling in MM cells. Moreover, we demonstrated the effect of metformin to be mainly oxygen dependent, since the HIF-1 pathway was not significantly affected by metformin in anoxic conditions as well as after application of hypoxic mimicking compound, CoCl Conclusions: Taken together, our study indicates metformin as a promising candidate for developing new treatment strategies exploiting HIF-1 signaling inhibition to enhance the overall anti-MM effect of currently used therapies, that may considerably benefit MM patients.
MeSH term(s)	Apoptosis/drug effects ; Cell Hypoxia/drug effects ; Cell Line, Tumor ; Humans ; Hypoxia-Inducible Factor 1/metabolism ; Metformin/pharmacology ; Multiple Myeloma/drug therapy ; Multiple Myeloma/metabolism ; Signal Transduction/drug effects ; Tumor Microenvironment/drug effects
Chemical Substances	Hypoxia-Inducible Factor 1 ; Metformin (9100L32L2N)
Language	English
Publishing date	2020-07-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2186248-5
ISSN	1734-1140
ISSN	1734-1140
DOI	10.1007/s43440-020-00142-x
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Article ; Online: Incorrect Analysis of MCAM Gene Promoter Methylation in Prostate Cancer.

Kocemba, Kinga A / Dudzik, Paulina / Ostrowska, Barbara / Laidler, Piotr

The Prostate

2016 Volume 76, Issue 15, Page(s) 1464–1465

Language	English
Publishing date	2016-11
Publishing country	United States
Document type	Letter
ZDB-ID	604707-5
ISSN	1097-0045 ; 0270-4137
ISSN (online)	1097-0045
ISSN	0270-4137
DOI	10.1002/pros.23210
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Article ; Online: Input of heavy metals to the forest floor as the result of zinc smelter pollution in southern Poland

Sawicka-Kapusta K. / Zakrzewska M. / Dudzik P. / Pietruszewska K.

E3S Web of Conferences, Vol 1, p

2013 Volume 19003

Abstract: The aim of this study was to assess the impact of zinc smelter on heavy metals concentration in the litter fall collected from three different sites in Olkusz Forest located in southern Poland. Furthermore the study was designed to investigate the input ... ...

Abstract	The aim of this study was to assess the impact of zinc smelter on heavy metals concentration in the litter fall collected from three different sites in Olkusz Forest located in southern Poland. Furthermore the study was designed to investigate the input of these metals to the forest floor. Three forest areas Podlesie I and Podlesie II located 3 and 4 km south-east from Bukowno zinc smelter and Cegielnia situated 6 km north-east from the smelter were selected. At the beginning of September 2006 five litter traps were set at each site. The quantity of litter fall, along with percentage composition of each species, were estimated for an area of 1m2. The concentration of Cd, Pb, Cu, Zn and Fe in the litter fall and leaves of four main tree species were analysed. The input of these metals to the forest floor were determined. The input of cadmium ranged from 204 μg m2 at Cegielnia to 1340 μg m2 at Podlesie I. The input of lead was higher than cadmium and varied from 1031 μg m2 at Cegielnia to 3252 μg m2 at Podlesie II.The input of zinc and iron were about 10 times than cadmium and lead. The less input of all invesigated metals was noticed in control site located at WNP. for those metals. The high input of heavy metals to the forest floor may have negative effects on investigated part of Olkusz forest ecosystem.
Keywords	zinc smelter ; heavy metals ; litter fall ; input ; forest floor ; Environmental sciences ; GE1-350
Subject code	550
Language	English
Publishing date	2013-04-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
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Article: Evaluation of Cysteine Metabolism in the Rat Liver and Kidney Following Intravenous Cocaine Administration and Abstinence.

Kowalczyk-Pachel, Danuta / Iciek, Małgorzata / Bilska-Wilkosz, Anna / Górny, Magdalena / Jastrzębska, Joanna / Kamińska, Kinga / Dudzik, Paulina / Filip, Małgorzata / Lorenc-Koci, Elżbieta

Antioxidants (Basel, Switzerland)

2021 Volume 10, Issue 1

Abstract: Many toxic effects of cocaine are attributed to reactive oxygen species (ROS) generated during its metabolism. Recently, it has been suggested that the biological action of ROS is often confused with endogenously generated reactive sulfur species (RSS). ... ...

Abstract	Many toxic effects of cocaine are attributed to reactive oxygen species (ROS) generated during its metabolism. Recently, it has been suggested that the biological action of ROS is often confused with endogenously generated reactive sulfur species (RSS). The aim of this study was to evaluate the impact of cocaine on thiols and RSS in the rat liver and kidney in the drug self-administration (SA) paradigm and the cocaine yoked delivery model (YC) followed by drug abstinence with extinction training. The level of thiols as well as RSS formed during anaerobic metabolism of cysteine and sulfate were assayed. In addition, the activity of enzymes involved in RSS formation and glutathione metabolism were determined. In the liver, following direct cocaine administration (SA and YC), the RSS levels decreased, while in the kidneys, cocaine increased the RSS contents in both groups. These changes were maintained in these tissues during drug abstinence. The level of sulfates was changed by cocaine only in the liver. In the kidney, cocaine shifted cysteine metabolism towards an anaerobic pathway. Our study demonstrates for the first time the changes in cysteine metabolism and thiol levels in the liver and kidney of rats after cocaine self-administration and abstinence.
Language	English
Publishing date	2021-01-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox10010074
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