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Article ; Online: A solution for inpatient ophthalmology coverage!

Khurshid, Syed Gibran / Duff, Sarah Madison

International journal of clinical practice

2021 Volume 75, Issue 10, Page(s) e14464

MeSH term(s)	Humans ; Inpatients ; Ophthalmology ; Referral and Consultation
Language	English
Publishing date	2021-09-17
Publishing country	England
Document type	Letter
ZDB-ID	1386246-7
ISSN	1742-1241 ; 1368-5031
ISSN (online)	1742-1241
ISSN	1368-5031
DOI	10.1111/ijcp.14464
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Article: Branch Retinal Vein Occlusion in a COVID-19 Positive Patient.

Duff, Sarah Madison / Wilde, Matthew / Khurshid, Gibran

Cureus

2021 Volume 13, Issue 2, Page(s) e13586

Abstract: Retinal vein occlusions (RVOs), including central retinal vein occlusions (CRVOs) and branch retinal vein occlusions (BRVOs), are a common cause of morbidity in elderly patients. We present the case of a healthy 74-year-old female patient who initially ... ...

Abstract	Retinal vein occlusions (RVOs), including central retinal vein occlusions (CRVOs) and branch retinal vein occlusions (BRVOs), are a common cause of morbidity in elderly patients. We present the case of a healthy 74-year-old female patient who initially presented with blurry vision in her left eye in the setting of a symptomatic COVID-19 infection. She was diagnosed with a branch retinal vein occlusion that did not immediately require treatment. Three months later, she again presented with worsening vision and was found to have cystoid macular edema (CME) secondary to the vein occlusion, thus was treated with an intravitreal dexamethasone implant. This case serves to highlight the growing evidence of increased thromboembolic risk associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the possible correlation of COVID-19 infections with ocular pathology, including retinal vein occlusions.
Language	English
Publishing date	2021-02-27
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.13586
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Article: Coluber Constrictor Bite to the Eye: A Novel Case Report of a Wild Snake Bite to the Eye in North America and Review of Literature.

Duff, Sarah Madison / Bowman, Andrew / Blake, Charles Richard

Cureus

2020 Volume 12, Issue 12, Page(s) e12125

Abstract: Snake bites involving the eye are an uncommon cause of ocular trauma; herein, we present one of the few known instances of a snake bite directly to the globe, specifically by the way of ... ...

Abstract	Snake bites involving the eye are an uncommon cause of ocular trauma; herein, we present one of the few known instances of a snake bite directly to the globe, specifically by the way of a
Language	English
Publishing date	2020-12-17
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.12125
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Article ; Online: The effects of coagulation factors and their inhibitors on proliferation and migration in colorectal cancer.

Rees, Peter Adam / Castle, John / Clouston, Hamish William / Lamb, Rebecca / Singh, Urvashi / Duff, Sarah Elizabeth / Kirwan, Cliona Clare

Cancer medicine

2023 Volume 12, Issue 16, Page(s) 17184–17192

Abstract: Background/aim: Clotting factors promote cancer development. We investigated if coagulation proteins promote proliferation and migration in colorectal cancer (CRC) cell lines and whether their direct inhibitors can attenuate these effects.: Materials ... ...

Abstract	Background/aim: Clotting factors promote cancer development. We investigated if coagulation proteins promote proliferation and migration in colorectal cancer (CRC) cell lines and whether their direct inhibitors can attenuate these effects. Materials and methods: DLD-1 and SW620 cells were treated with tissue factor (0, 50, 100 and 500 pg/mL ± 10 μg/mL 10H10 [anti-tissue factor antibody]), thrombin (0.0, 0.1, 1.0 and 10.0 U/mL ± 0.5 μM dabigatran [thrombin inhibitor]) and Factor Xa, FXa (0.0, 0.1, 1.0 and 10.0 U/mL ± 100 ng/mL rivaroxaban [FXa inhibitor]) and their effects on proliferation and migration were quantified using the PrestoBlue® and transwell migration assays, respectively. Results: Thrombin increased proliferation from 48 h treatment compared to its control (48 h 6.57 ± 1.36 u vs. 2.42 ± 0.13 u, p = 0.001, 72 h 9.50 ± 1.54 u vs. 4.50 ± 0.47 u, p = 0.004 and 96 h 10.77 ± 1.72 u vs. 5.57 ± 0.25 u, p = 0.008). This increase in proliferation was attenuated by dabigatran at 72 h (2.23 ± 0.16 u vs. 3.26 ± 0.43 u, p = 0.04). Tissue factor (0 pg/mL 20.7 ± 1.6 cells/view vs. 50 pg/mL 32.4 ± 1.9 cells/view, p = 0.0002), FXa (0.0 U/mL 8.9 ± 1.1 cells/view vs. 10.0 U/mL 17.7 ± 1.7 cells/view, p < 0.0001) and thrombin (0.0 U/mL 8.9 ± 1.3 cells/view vs. 10.0 U/mL 20.2 ± 2.0 cells/view, p < 0.0001) all increased migration compared to their controls. However, their direct inhibitors did not attenuate these increases. Conclusion: Thrombin, FXa and TF all increase migration in CRC in vitro. Thrombin induced increase in proliferation is abrogated by dabigatran. Dabigatran may have potential as an anti-cancer therapy in CRC.
MeSH term(s)	Humans ; Dabigatran/pharmacology ; Dabigatran/therapeutic use ; Thrombin/metabolism ; Factor Xa Inhibitors/pharmacology ; Blood Coagulation Factors/pharmacology ; Thromboplastin/metabolism ; Colorectal Neoplasms/drug therapy ; Cell Proliferation
Chemical Substances	Dabigatran (I0VM4M70GC) ; Thrombin (EC 3.4.21.5) ; Factor Xa Inhibitors ; Blood Coagulation Factors ; Thromboplastin (9035-58-9)
Language	English
Publishing date	2023-07-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2659751-2
ISSN	2045-7634 ; 2045-7634
ISSN (online)	2045-7634
ISSN	2045-7634
DOI	10.1002/cam4.6332
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Article ; Online: A Prospective Study to Identify Rates of SARS-CoV-2 Virus in the Peritoneum and Lower Genital Tract of Patients Having Surgery: An Observational Study.

Jones, Dominique / Faluyi, David / Hamilton, Sarah / Stylianides, Nicholas / Ma, Ken / Duff, Sarah / Machin, Nicholas / Edmondson, Richard J

Journal of minimally invasive gynecology

2021 Volume 28, Issue 9, Page(s) 1633–1636

Abstract: Study objective: The risks to surgeons of carrying out aerosol-generating procedures during the coronavirus disease 2019 (COVID-19) pandemic are unknown. To start to define these risks, in a systematic manner, we investigated the presence of severe ... ...

Abstract	Study objective: The risks to surgeons of carrying out aerosol-generating procedures during the coronavirus disease 2019 (COVID-19) pandemic are unknown. To start to define these risks, in a systematic manner, we investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus in the abdominal fluid and lower genital tract of patients undergoing surgery. Design: Prospective cross-sectional observational study. Setting: Single, large United Kingdom hospital. Patients: Total of 113 patients undergoing abdominal surgery or instrumentation of the lower genital tract. Interventions: We took COVID-19 swabs from the peritoneal cavity and from the vagina from all eligible patients. Results were stratified by preoperative COVID-19 status. Measurements and main results: In patients who were presumed COVID-19 negative at the time of surgery, SARS-CoV-2 virus RNA was detected in 0 of 102 peritoneal samples and 0 of 98 vaginal samples. Both cohorts included 4 patients who were antibody positive but nasopharyngeal swab test negative at the time of surgery. Peritoneal and vaginal swabs were also negative in 1 patient who had a positive nasopharyngeal swab immediately before surgery. Conclusion: The presence of SARS-CoV-2 RNA in the abdominal fluid or lower genital tract of presumed negative patients is nil or extremely low. These data will inform surgeons of the risks of restarting laparoscopic surgery at a time when COVID-19 is endemic in the population.
MeSH term(s)	COVID-19 ; Cross-Sectional Studies ; Female ; Humans ; Peritoneum ; Prospective Studies ; RNA, Viral ; SARS-CoV-2 ; Vagina
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-02-11
Publishing country	United States
Document type	Journal Article ; Observational Study
ZDB-ID	2186934-0
ISSN	1553-4669 ; 1553-4650
ISSN (online)	1553-4669
ISSN	1553-4650
DOI	10.1016/j.jmig.2021.02.006
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Article: Treatment of Coats' disease: an analysis of pooled results.

Adeniran, Janelle Fassbender / Duff, Sarah M / Mimouni, Michael / Lambert, Nathan / Ramasubramanian, Aparna

International journal of ophthalmology

2019 Volume 12, Issue 4, Page(s) 668–674

Abstract: Aim: To elucidate the association of treatment modality to vitreoretinal fibrosis and traction retinal detachment (TRD) in Coats' disease.: Methods: A PubMed search for Coats' disease with included studies describing eyes with clinical features and ... ...

Abstract	Aim: To elucidate the association of treatment modality to vitreoretinal fibrosis and traction retinal detachment (TRD) in Coats' disease. Methods: A PubMed search for Coats' disease with included studies describing eyes with clinical features and treatment course of Coats' disease. Binary logistic regression with fibrosis at presentation and treatment type as independent variables was performed to determine predictors of TRD historically (since 1921) and in the anti-vascular endothelial growth factor (VEGF) era (since 2007). Odds ratios (OR) with 95% confidence intervals (CI) reported. Results: Of 175 articles described 1183 eyes. Vitreoretinal fibrosis increased from presentation (5.4%) to follow-up (15.5%) and TRD increased from 0.44% to 3.9% at follow up. Laser was protective against vitreoretinal fibrosis (OR 0.6, 95%CI 0.4-0.9) but TRD was borderline (OR 0.6, 95%CI 0.3-1.1). Cryotherapy showed a higher association with TRD (OR 1.9, 95%CI 1.0-3.7) than with vitreoretinal fibrosis (OR 0.8, 95%CI 0.5-1.2). Similarly, intravitreal anti-VEGF alone was not associated with fibrosis (OR 1.1, 95%CI 0.6-1.8) nor TRD (OR 1.1, 95%CI 0.5-2.6) but the combination of laser and anti-VEGF therapy was protective [Fibrosis: 0.1 (0.03, 0.35); TRD: 0.05 (0.01, 0.23)] compared to anti-VEGF plus cryotherapy ( Conclusion: Vitreoretinal fibrosis and TRD increase after treatment in Coats' disease. The combination of anti-VEGF agents and cryotherapy may lead to higher risk for TRD. Presence of pre-treatment fibrosis is the highest risk factor for post-treatment worsening of vitreoretinal fibrosis and TRD.
Language	English
Publishing date	2019-04-18
Publishing country	China
Document type	Journal Article ; Review
ZDB-ID	2663246-9
ISSN	2227-4898 ; 2222-3959
ISSN (online)	2227-4898
ISSN	2222-3959
DOI	10.18240/ijo.2019.04.23
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Article ; Online: Effect of Tissue Factor on Colorectal Cancer Stem Cells.

Clouston, Hamish William / Rees, Peter Adam / Lamb, Rebecca / Duff, Sarah Elizabeth / Kirwan, Cliona Clare

Anticancer research

2018 Volume 38, Issue 5, Page(s) 2635–2642

Abstract: Background/aim: Tissue factor (TF) expression increases cancer stem cell (CSC) activity in breast and lung cancer. There are ongoing studies focused on targeting CSCs via anti-TF treatment, for breast and lung cancer therapy. Herein, the aim was to ... ...

Abstract	Background/aim: Tissue factor (TF) expression increases cancer stem cell (CSC) activity in breast and lung cancer. There are ongoing studies focused on targeting CSCs via anti-TF treatment, for breast and lung cancer therapy. Herein, the aim was to determine whether targeting TF could have an anti-CSC therapeutic role in colorectal cancer (CRC). Materials and methods: Evaluation of colonosphere-forming efficiency (CFE) and aldehyde dehydrogenase (ALDH) expression level was used to quantify CSC activity in two CRC cell lines, after TF knockdown (TFKD) or TF over-expression (TFOE). Results: TFKD resulted in increased levels of ALDH in SW620 (1.31±0.04-fold, p<0.001) and DLD-1 (1.63±0.14-fold, p=0.04) cells. CFE was increased in SW620 (1.21±0.23% vs. 2.03±0.29%, p=0.01) and DLD-1 (0.41±0.12% vs. 0.68±0.9%, p=0.01) cells. Conversely, TFOE decreased ALDH expression (0.72±0.04-fold, p=0.001) and CFE (0.33±0.05% vs. 0.66±0.14%, p=0.006) in DLD-1, but had no impact on SW620 cells. Conclusion: In the examined CRC cell lines, TF expression was inversely related to CSC activity suggesting that anti-TF therapies may not have a role in CRC treatment.
MeSH term(s)	Aldehyde Dehydrogenase/analysis ; Biomarkers, Tumor ; Cell Division ; Cell Line, Tumor ; Colorectal Neoplasms/pathology ; Gene Knockdown Techniques ; Genetic Vectors/pharmacology ; Humans ; Lentivirus/genetics ; Neoplastic Stem Cells/metabolism ; Neoplastic Stem Cells/pathology ; RNA Interference ; RNA, Small Interfering/genetics ; Recombinant Proteins/metabolism ; Spheroids, Cellular ; Thromboplastin/antagonists & inhibitors ; Thromboplastin/genetics ; Thromboplastin/physiology
Chemical Substances	Biomarkers, Tumor ; RNA, Small Interfering ; Recombinant Proteins ; Thromboplastin (9035-58-9) ; Aldehyde Dehydrogenase (EC 1.2.1.3)
Language	English
Publishing date	2018-05-01
Publishing country	Greece
Document type	Journal Article
ZDB-ID	604549-2
ISSN	1791-7530 ; 0250-7005
ISSN (online)	1791-7530
ISSN	0250-7005
DOI	10.21873/anticanres.12505
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Article ; Online: Hairpulling causing vision loss: a case report.

Puri, Sidharth / Duff, Sarah Madison / Mueller, Brett / Prendes, Mark / Clark, Jeremy

Orbit (Amsterdam, Netherlands)

2018 Volume 38, Issue 2, Page(s) 162–165

Abstract: Subperiosteal extension of a subgaleal hematoma (SGH) to the orbit is a reported, but rare complication of trauma. This report details a 13-year-old African-American male who originally presented to the emergency department after trauma with headache and ...

Abstract	Subperiosteal extension of a subgaleal hematoma (SGH) to the orbit is a reported, but rare complication of trauma. This report details a 13-year-old African-American male who originally presented to the emergency department after trauma with headache and was found on CT imaging to have a contained subgaleal hemorrhage. He presented 2 days later with increased pain and proptosis of the left eye with findings of decreased visual acuity, elevated intraocular pressure, proptosis, and complete external ophthalmoplegia. Repeat imaging revealed enlargement of the SGH with subperiosteal extension into the left orbit. He required an emergent lateral canthotomy with inferior and superior cantholysis, followed by surgical drainage of the subperiosteal and SGH. Hematologic workup for coagulopathy was negative. The authors urge point-of-care providers to consider ophthalmic evaluation for patients with large SGHs where orbital extension and vision loss may occur. Furthermore, SGH causing orbital compartment syndrome may develop in patients who have normal blood work and clotting factors.
MeSH term(s)	Adolescent ; Blindness/etiology ; Compartment Syndromes/diagnostic imaging ; Compartment Syndromes/etiology ; Compartment Syndromes/surgery ; Drainage/methods ; Hair Follicle/injuries ; Hematoma/diagnostic imaging ; Hematoma/etiology ; Hematoma/surgery ; Humans ; Male ; Ophthalmologic Surgical Procedures ; Orbit/surgery ; Orbital Diseases/diagnostic imaging ; Orbital Diseases/etiology ; Orbital Diseases/surgery ; Tomography, X-Ray Computed ; Wounds and Injuries/etiology
Language	English
Publishing date	2018-05-04
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	603157-2
ISSN	1744-5108 ; 0167-6830
ISSN (online)	1744-5108
ISSN	0167-6830
DOI	10.1080/01676830.2018.1459739
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Article ; Online: Managing surgical demand when needs outstrip resource: qualitative investigation of colorectal cancer surgery provision in the first wave of the COVID-19 pandemic.

Conefrey, Carmel / Ochieng, Cynthia / Hoffman, Christin / Elliott, Daisy / Avery, Kerry / Bennett, Joanne / Blencowe, Natalie / Duff, Sarah / Kinross, James / McNair, Angus / Messenger, David / Pullybank, Anne / Singh, Baljit / King, Anni / Squire, Sarah E / Blazeby, Jane / Main, Barry / Rooshenas, Leila

The British journal of surgery

2022 Volume 110, Issue 1, Page(s) 92–97

Abstract: Background: At the onset of the COVID-19 pandemic, elective surgical provision was severely affected by the need for hospital reorganization to care for critically ill patients. In response, National Health Service (NHS) England issued national guidance ...

Abstract	Background: At the onset of the COVID-19 pandemic, elective surgical provision was severely affected by the need for hospital reorganization to care for critically ill patients. In response, National Health Service (NHS) England issued national guidance proposing acceptable time intervals for postponing different types of surgical procedure. This study reports healthcare professionals' private accounts of the strategies adopted to manage the imbalance of demand and resource, using colorectal cancer surgery as a case study. Methods: Twenty-seven semistructured interviews were conducted with healthcare professionals between June and November 2020. A key informant sampling approach was used, followed by snowballing to achieve maximum regional variation across the UK. Data were analysed thematically using the constant comparison approach. Results: In the context of considerable resource constraint, surgical teams overcame challenges to continue elective cancer provision. They achieved this by pursuing a combination of strategies: relocating surgical services; prioritizing patients within and across surgical specialties; adapting patient treatment plans; and introducing changes to surgical team working practices. Despite national guidance, prioritization decisions were framed as complex, and the most challenging of the strategies to implement, both practically and emotionally. Conclusion: There is a need to better support surgeons tasked with prioritizing patients when capacity exceeds demand.
MeSH term(s)	Humans ; COVID-19/epidemiology ; Pandemics ; State Medicine ; Elective Surgical Procedures ; Colorectal Neoplasms/surgery
Language	English
Publishing date	2022-11-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2985-3
ISSN	1365-2168 ; 0263-1202 ; 0007-1323 ; 1355-7688
ISSN (online)	1365-2168
ISSN	0263-1202 ; 0007-1323 ; 1355-7688
DOI	10.1093/bjs/znac371
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Article ; Online: The Association of Coloproctology of Great Britain and Ireland consensus guidelines in emergency colorectal surgery.

Miller, Andrew S / Boyce, Kathryn / Box, Benjamin / Clarke, Matthew D / Duff, Sarah E / Foley, Niamh M / Guy, Richard J / Massey, Lisa H / Ramsay, George / Slade, Dominic A J / Stephenson, James A / Tozer, Phil J / Wright, Danette

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland

2021 Volume 23, Issue 2, Page(s) 476–547

Abstract: Aim: There is a requirement for an expansive and up to date review of the management of emergency colorectal conditions seen in adults. The primary objective is to provide detailed evidence-based guidelines for the target audience of general and ... ...

Abstract	Aim: There is a requirement for an expansive and up to date review of the management of emergency colorectal conditions seen in adults. The primary objective is to provide detailed evidence-based guidelines for the target audience of general and colorectal surgeons who are responsible for an adult population and who practise in Great Britain and Ireland. Methods: Surgeons who are elected members of the Association of Coloproctology of Great Britain and Ireland Emergency Surgery Subcommittee were invited to contribute various sections to the guidelines. They were directed to produce a pathology-based document using literature searches that were systematic, comprehensible, transparent and reproducible. Levels of evidence were graded. Each author was asked to provide a set of recommendations which were evidence-based and unambiguous. These recommendations were submitted to the whole guideline group and scored. They were then refined and submitted to a second vote. Only those that achieved >80% consensus at level 5 (strongly agree) or level 4 (agree) after two votes were included in the guidelines. Results: All aspects of care (excluding abdominal trauma) for emergency colorectal conditions have been included along with 122 recommendations for management. Conclusion: These guidelines provide an up to date and evidence-based summary of the current surgical knowledge in the management of emergency colorectal conditions and should serve as practical text for clinicians managing colorectal conditions in the emergency setting.
MeSH term(s)	Colorectal Surgery ; Consensus ; Digestive System Surgical Procedures ; Emergency Service, Hospital ; Humans ; United Kingdom
Language	English
Publishing date	2021-01-22
Publishing country	England
Document type	Journal Article
ZDB-ID	1440017-0
ISSN	1463-1318 ; 1462-8910
ISSN (online)	1463-1318
ISSN	1462-8910
DOI	10.1111/codi.15503
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