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  1. Article: Characterization of Levan Fructan Produced by a

    Bruni, Gillian O / Qi, Yunci / Terrell, Evan / Dupre, Rebecca A / Mattison, Christopher P

    Microorganisms

    2024  Volume 12, Issue 1

    Abstract: During raw sugarcane processing, a significant portion of lost sucrose is attributable to microbial degradation. Sucrose consumption by many bacteria is also linked to the production of exopolysaccharides (EPS) such as dextrans and fructans. These ... ...

    Abstract During raw sugarcane processing, a significant portion of lost sucrose is attributable to microbial degradation. Sucrose consumption by many bacteria is also linked to the production of exopolysaccharides (EPS) such as dextrans and fructans. These resulting EPS cause operational challenges during raw sugar manufacturing. Here, we report the characterization of EPS from a fructan-forming
    Language English
    Publishing date 2024-01-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms12010107
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  2. Article ; Online: Dietary uptake of geosmin in rainbow trout (Oncorhynchus mykiss)

    Dupre, Rebecca A. / Ardoin, Ryan / Trushenski, Jesse / Jackson, Chris / Grimm, Casey / Smith, Brennan

    Aquaculture. 2023 June, v. 571 p.739458-

    2023  

    Abstract: Geosmin is a primary source of muddy/earthy ‘off-flavors’ in farmed fish, which may render their organoleptic quality unacceptable to consumers. Model systems of geosmin uptake typically comprise exposure to waterborne geosmin that is absorbed via the ... ...

    Abstract Geosmin is a primary source of muddy/earthy ‘off-flavors’ in farmed fish, which may render their organoleptic quality unacceptable to consumers. Model systems of geosmin uptake typically comprise exposure to waterborne geosmin that is absorbed via the gills. The present research demonstrates dietary exposure as an alternative route of geosmin uptake in Rainbow Trout (Oncorhynchus mykiss) fillets. Trout (average initial weight of 355 g) were stocked in quadruplicate compartmentalized raceways (N = 4 compartments per treatment, n = 50 fish per compartment) supplied with first-use, flow-through water (4.5 complete turnovers per hour). Fish were fed diets containing 0 (control dose), 0.005 (low dose), 0.05 (medium dose), or 0.5 (high dose) mg geosmin/kg feed at 1% body weight/day for four weeks. Fillets (12 fillets/diet/week plus 12 pre-trial fillet samples) and weekly feed and water samples were analyzed via GC–MS to determine geosmin concentrations. Feeding behavior was documented daily according to a four-point scale. ANOVA with post-hoc Tukey tests and polynomial contrast, regression, correlation, and chi-squared statistical analyses were applied to data (α = 0.05 significance level). Palatability of feed did not hinder consumption of the geosmin-spiked feeds: although fish responded positively to all feeds, the most aggressive feeding behavior was observed among those fed medium and high dose feed. Geosmin was effectively imparted into fillets after one week, and no significant temporal effect was found after four weeks. Mean geosmin concentrations significantly increased in fillets from low (mean of 26 ng/kg geosmin) to medium (202 ng/kg) to high (441 ng/kg) dose feed groups during the trial. Polynomial contrasts and regression modeling validated this significant positive effect of dose on geosmin uptake, with an estimated 166 ng/kg rise in fillet-geosmin for every log₁₀ increase in feed concentration. Waterborne geosmin levels immediately post-feeding were conditionally independent of concentrations in feed and fillets, therefore dietary uptake was affirmed as the predominant mechanism of absorption in the present experimental system. Based on these findings, geosmin-spiked feeds may be used to induce predictable, repeatable levels of this off-flavor compound in fillets and serve as a model system for further investigation of sensory quality and off-flavor mitigation strategies for farm-raised fish.
    Keywords Oncorhynchus mykiss ; absorption ; aquaculture ; body weight ; diet ; dietary exposure ; farmed fish ; fillets ; geosmin ; off flavors ; palatability ; Rainbow trout ; Off-flavor
    Language English
    Dates of publication 2023-06
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2023.739458
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    In stock of ZB MED Bonn / Germany

    Z 3275: Show issues

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  3. Article ; Online: Assessment of Cellulose Nanofiber-Based Elastase Biosensors to Inflammatory Disease as a Function of Spacer Length and Fluorescence Response.

    Easson, Michael W / Jordan, Jacobs H / Edwards, J Vincent / Prevost, Nicolette T / Dupre, Rebecca A / Hillyer, Matthew B / Lima, Isabel M / Nam, Sunghyun

    ACS applied bio materials

    2024  Volume 7, Issue 3, Page(s) 1490–1500

    Abstract: Inflammatory disease biomarker detection has become a high priority in point-of-care diagnostic research in relation to chronic wounds, with a variety of sensor-based designs becoming available. Herein, two primary aspects of biosensor design are ... ...

    Abstract Inflammatory disease biomarker detection has become a high priority in point-of-care diagnostic research in relation to chronic wounds, with a variety of sensor-based designs becoming available. Herein, two primary aspects of biosensor design are examined: (1) assessment of a cellulose nanofiber (CNF) matrix derived from cotton ginning byproducts as a sensor transducer surface; and (2) assessment of the relation of spacer length and morphology between the CNF cellulose backbone and peptide fluorophore as a function of sensor activity for porcine pancreatic and human neutrophil elastases. X-ray crystallography, specific surface area, and pore size analyses confirmed the suitability of CNF as a matrix for wound care diagnostics. Based upon the normalized degree of substitution, a pegylated-linker connecting CNF transducer substrate to peptide fluorophore showed the greatest fluorescence response, compared to short- and long-chain alkylated linkers.
    MeSH term(s) Animals ; Swine ; Humans ; Nanofibers ; Cellulose/chemistry ; Peptides/chemistry ; Biosensing Techniques
    Chemical Substances Cellulose (9004-34-6) ; Peptides
    Language English
    Publishing date 2024-02-20
    Publishing country United States
    Document type Journal Article
    ISSN 2576-6422
    ISSN (online) 2576-6422
    DOI 10.1021/acsabm.3c00885
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  4. Article ; Online: A subset of leukocyte immune-type receptors (LITRs) regulates phagocytosis in channel catfish (Ictalurus punctatus) leukocytes

    Crider, Jonathan / Wilson, Melanie / Felch, Kristianna L. / Dupre, Rebecca A. / Quiniou, Sylvie M.A. / Bengtén, Eva

    Molecular Immunology. 2023 Feb., v. 154 p.33-44

    2023  

    Abstract: Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; ...

    Abstract Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; however, LITR-mediated phagocytosis has not been examined in the catfish model. In this study, we use two anti-LITR monoclonal antibodies, CC41 and 125.2, to contrast the effects of crosslinking subsets of inhibitory and activating LITRs. Briefly, LITRs expressed by catfish γδ T cells, αβ T cells, and macrophage cell lines were crosslinked using mAb-conjugated fluorescent microbeads, and bead uptake was evaluated by flow cytometry and confirmed by confocal microscopy. A clear difference in the uptake of 125.2- and CC41-conjugated beads was observed. Crosslinking LITRs with mAb 125.2 resulted in efficient bead internalization, while mAb CC41 crosslinking of inhibitory LITRs resulted predominantly in a capturing phenotype. Pretreating catfish macrophages with mAb CC41 resulted in a marked decrease in LITR-mediated phagocytosis of 125.2-conjugated beads. Overall, these findings provide insight into fish immunobiology and validate LITRs as regulators of phagocytosis in catfish macrophages and γδ T cells.
    Keywords Ictalurus punctatus ; catfish ; confocal microscopy ; crosslinking ; flow cytometry ; fluorescence ; immunomodulation ; leukocytes ; macrophages ; mammals ; microbeads ; models ; phagocytosis ; phenotype ; APC ; μME ; BSA ; cfPBS ; cfRPMI ; CCV ; CEACAM ; Csk ; CTL ; CYT ; DAP10 ; DAP12 ; DICP ; FcR ; FcRL ; HA ; Ig ; IgSF ; ITAM ; ITIM ; ITSM ; KIR ; LAK ; LILR ; LITR ; MHC ; MLC ; nanoLC-MS/MS ; Nck ; NILT ; NITR ; NK ; NKG2D ; PBL ; PIGRL ; RBL ; TM ; TREM ; SHP-2 ; Siglec ; SIRP ; WGA ; Paired receptors ; Immunoregulatory receptors ; Inhibitory signaling ; Cytoplasmic tails ; Innate immunity ; Leukocyte immune-type receptors ; Channel catfish
    Language English
    Dates of publication 2023-02
    Size p. 33-44.
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2022.12.009
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 166: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: A subset of leukocyte immune-type receptors (LITRs) regulates phagocytosis in channel catfish (Ictalurus punctatus) leukocytes.

    Crider, Jonathan / Wilson, Melanie / Felch, Kristianna L / Dupre, Rebecca A / Quiniou, Sylvie M A / Bengtén, Eva

    Molecular immunology

    2022  Volume 154, Page(s) 33–44

    Abstract: Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; ...

    Abstract Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; however, LITR-mediated phagocytosis has not been examined in the catfish model. In this study, we use two anti-LITR monoclonal antibodies, CC41 and 125.2, to contrast the effects of crosslinking subsets of inhibitory and activating LITRs. Briefly, LITRs expressed by catfish γδ T cells, αβ T cells, and macrophage cell lines were crosslinked using mAb-conjugated fluorescent microbeads, and bead uptake was evaluated by flow cytometry and confirmed by confocal microscopy. A clear difference in the uptake of 125.2- and CC41-conjugated beads was observed. Crosslinking LITRs with mAb 125.2 resulted in efficient bead internalization, while mAb CC41 crosslinking of inhibitory LITRs resulted predominantly in a capturing phenotype. Pretreating catfish macrophages with mAb CC41 resulted in a marked decrease in LITR-mediated phagocytosis of 125.2-conjugated beads. Overall, these findings provide insight into fish immunobiology and validate LITRs as regulators of phagocytosis in catfish macrophages and γδ T cells.
    MeSH term(s) Animals ; Ictaluridae/genetics ; Ictaluridae/metabolism ; Receptors, Immunologic ; Phagocytosis ; Leukocytes ; Catfishes ; Mammals
    Chemical Substances Receptors, Immunologic
    Language English
    Publishing date 2022-12-29
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2022.12.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 166: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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