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Article ; Online: The use of agitated saline as contrast agent in a contrast‐enhanced cystosonography for detection of urinary bladder rupture in animal cadavers

Muehlbauer, Eloisa / Saldanha, André / Gil, Elaine M. / Alves, Thais L. / Duque, Juan C. M. / Froes, Tilde R.

Journal of Veterinary Emergency and Critical Care. 2023 , v. 33, no. 3 p.327-331

2023

Abstract: OBJECTIVE: To determine the value of microbubble contrast cystosonography in the diagnosis of bladder rupture in animals. DESIGN: Prospective, method comparison study from November 2019 to October 2020. SETTING: University teaching hospital. ANIMALS: ... ...

Abstract	OBJECTIVE: To determine the value of microbubble contrast cystosonography in the diagnosis of bladder rupture in animals. DESIGN: Prospective, method comparison study from November 2019 to October 2020. SETTING: University teaching hospital. ANIMALS: Thirty‐four ethically sourced cadavers of dogs, rats, and rabbits. INTERVENTIONS: In a prospective and blinded study, the cadavers were divided into 2 randomized groups: with bladder rupture (CR), and without bladder rupture (SR). Urinary catheterization was performed in all cadavers. Through the urethral catheter, bladders in CR group were ruptured using a rigid stainless steel guide wire. Microbubble contrast was infused into the bladder through the urethral catheter, while a single, blinded observer sonographically assessed the bladder. The time to diagnosis and the number of attempts needed for diagnosis were recorded. MEASUREMENTS AND MAIN RESULTS: The study included cadavers of 16 female Wistar rats, 6 female dogs, 11 male dogs, and 1 male rabbit. Time to diagnosis in dogs (2.25 ± 0.91 min) was statistically higher when compared to rats (1.15 ± 0.75 min; P = 0.03). Of the 34 cases, incorrect diagnosis of bladder rupture was made in only 2 dogs (6%), indicating a diagnostic sensitivity of 88.88%, specificity of 100%, and an accuracy of 94%. The positive predictive value was 1 and the negative predictive value was 0.9. CONCLUSIONS: Our study showed that the described method is accurate, sensitive, and specific for the detection of bladder rupture in animal cadavers of different species, size, and sex.
Keywords	bladder ; catheters ; diagnostic sensitivity ; females ; hospitals ; males ; microbubbles ; rabbits ; stainless steel
Language	English
Dates of publication	2023-05
Size	p. 327-331.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2077212-9
ISSN	1476-4431 ; 1479-3261
ISSN (online)	1476-4431
ISSN	1479-3261
DOI	10.1111/vec.13297
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Sedative effects of an intramuscular or intranasal combination of sufentanil and midazolam in New Zealand White rabbits.

Freitag, Flavio A V / Ferreira, Ariele A / Teixeira, William T / Kerr, Carolyn L / Duque, Juan C M

Veterinary anaesthesia and analgesia

2022 Volume 49, Issue 5, Page(s) 468–472

Abstract: Objective: To evaluate the sedative effects of a combination of sufentanil and midazolam administered intramuscularly (IM) or intranasally (IN) prior to induction of anesthesia with propofol in New Zealand White rabbits.: Study design: Prospective, ... ...

Abstract	Objective: To evaluate the sedative effects of a combination of sufentanil and midazolam administered intramuscularly (IM) or intranasally (IN) prior to induction of anesthesia with propofol in New Zealand White rabbits. Study design: Prospective, randomized, crossover, experimental study. Animals: A total of 11 adult New Zealand White rabbits. Methods: Sufentanil (0.5 μg kg Results: Measured variables remained within normal physiologic ranges for all rabbits. The only statistically significant change was for f Conclusions and clinical relevance: Sufentanil combined with midazolam administered either IM or IN resulted in moderate to deep sedation in New Zealand White rabbits at the dose rates studied.
MeSH term(s)	Animals ; Hypnotics and Sedatives/pharmacology ; Midazolam/pharmacology ; Propofol ; Prospective Studies ; Rabbits ; Sufentanil/pharmacology
Chemical Substances	Hypnotics and Sedatives ; Sufentanil (AFE2YW0IIZ) ; Midazolam (R60L0SM5BC) ; Propofol (YI7VU623SF)
Language	English
Publishing date	2022-04-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	2020582-X
ISSN	1467-2995 ; 1467-2987
ISSN (online)	1467-2995
ISSN	1467-2987
DOI	10.1016/j.vaa.2022.04.006
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Article ; Online: The use of agitated saline as contrast agent in a contrast-enhanced cystosonography for detection of urinary bladder rupture in animal cadavers.

Muehlbauer, Eloisa / Saldanha, André / Gil, Elaine M / Alves, Thais L / Duque, Juan C M / Froes, Tilde R

Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)

2023 Volume 33, Issue 3, Page(s) 327–331

Abstract: Objective: To determine the value of microbubble contrast cystosonography in the diagnosis of bladder rupture in animals.: Design: Prospective, method comparison study from November 2019 to October 2020.: Setting: University teaching hospital.: ... ...

Abstract	Objective: To determine the value of microbubble contrast cystosonography in the diagnosis of bladder rupture in animals. Design: Prospective, method comparison study from November 2019 to October 2020. Setting: University teaching hospital. Animals: Thirty-four ethically sourced cadavers of dogs, rats, and rabbits. Interventions: In a prospective and blinded study, the cadavers were divided into 2 randomized groups: with bladder rupture (CR), and without bladder rupture (SR). Urinary catheterization was performed in all cadavers. Through the urethral catheter, bladders in CR group were ruptured using a rigid stainless steel guide wire. Microbubble contrast was infused into the bladder through the urethral catheter, while a single, blinded observer sonographically assessed the bladder. The time to diagnosis and the number of attempts needed for diagnosis were recorded. Measurements and main results: The study included cadavers of 16 female Wistar rats, 6 female dogs, 11 male dogs, and 1 male rabbit. Time to diagnosis in dogs (2.25 ± 0.91 min) was statistically higher when compared to rats (1.15 ± 0.75 min; P = 0.03). Of the 34 cases, incorrect diagnosis of bladder rupture was made in only 2 dogs (6%), indicating a diagnostic sensitivity of 88.88%, specificity of 100%, and an accuracy of 94%. The positive predictive value was 1 and the negative predictive value was 0.9. Conclusions: Our study showed that the described method is accurate, sensitive, and specific for the detection of bladder rupture in animal cadavers of different species, size, and sex.
MeSH term(s)	Male ; Female ; Rats ; Dogs ; Animals ; Rabbits ; Urinary Bladder/diagnostic imaging ; Contrast Media ; Prospective Studies ; Rats, Wistar ; Urinary Bladder Diseases/diagnostic imaging ; Urinary Bladder Diseases/veterinary ; Abdominal Injuries/veterinary ; Thoracic Injuries/veterinary ; Dog Diseases
Chemical Substances	Contrast Media
Language	English
Publishing date	2023-04-23
Publishing country	United States
Document type	Randomized Controlled Trial, Veterinary ; Journal Article
ZDB-ID	2077212-9
ISSN	1476-4431 ; 1479-3261
ISSN (online)	1476-4431
ISSN	1479-3261
DOI	10.1111/vec.13297
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Article: Sedative effects of an intramuscular or intranasal combination of sufentanil and midazolam in New Zealand White rabbits

Freitag, Flavio A.V. / Ferreira, Ariele A. / Teixeira, William T. / Kerr, Carolyn L. / Duque, Juan C.M.

Veterinary anaesthesia and analgesia. 2022 Apr. 21,

2022

Abstract: To evaluate the sedative effects of a combination of sufentanil and midazolam administered intramuscularly (IM) or intranasally (IN) prior to induction of anesthesia with propofol in New Zealand White rabbits. Prospective, randomized, crossover, ... ...

Abstract	To evaluate the sedative effects of a combination of sufentanil and midazolam administered intramuscularly (IM) or intranasally (IN) prior to induction of anesthesia with propofol in New Zealand White rabbits. Prospective, randomized, crossover, experimental study. A total of 11 adult New Zealand White rabbits. Sufentanil (0.5 μg kg⁻¹) and midazolam (2 mg kg⁻¹) were administered to rabbits by either IM or IN route. The righting reflex was assessed, and sedation was scored. Heart rate (HR), respiratory rate (fR) and temperature (T) were recorded prior to treatment administration and after loss of the righting reflex. Measured variables remained within normal physiologic ranges for all rabbits. The only statistically significant change was for fR, which was significantly lower after sedation for both routes. The time to loss of righting reflex was 14.8 ± 6.5 and 12.5 ± 7.4 minutes and sedation scores were 6 (4–8) and 7 (6–8) for IM and IN routes respectively, with no difference between treatments. No adverse effects were observed during the experimental period. Sufentanil combined with midazolam administered either IM or IN resulted in moderate to deep sedation in New Zealand White rabbits at the dose rates studied.
Keywords	adults ; analgesia ; anesthesia ; heart rate ; respiratory rate ; sedation ; sedatives ; temperature
Language	English
Dates of publication	2022-0421
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	2020582-X
ISSN	1467-2995 ; 1467-2987
ISSN (online)	1467-2995
ISSN	1467-2987
DOI	10.1016/j.vaa.2022.04.006
Database	NAL-Catalogue (AGRICOLA)

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Article: Evaluation of injection volumes for the transversus abdominis plane block in dog cadavers: a preliminary trial

Freitag, Flavio A.V / Muehlbauer, Eloisa / Gaio, Thalise daS / dos Santos, Amanda A.M / Machado, Marcello / Sanchez, Andrea / Duque, Juan C.M

Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia Veterinary anaesthesia and analgesia. 2021 Jan., v. 48, no. 1

2021

Abstract: To evaluate staining of nerve branches after the injection of different volumes during ultrasound-guided transversus abdominis plane (TAP) block in dog cadavers.Prospective randomized study.A total of 15 frozen/thawed adult dog cadavers.Hemiabdomens were ...

Abstract	To evaluate staining of nerve branches after the injection of different volumes during ultrasound-guided transversus abdominis plane (TAP) block in dog cadavers.Prospective randomized study.A total of 15 frozen/thawed adult dog cadavers.Hemiabdomens were randomly allocated to one of four groups. In groups G0.3, G0.6 and G1.0, ropivacaine–methylene blue solution (0.3, 0.6 and 1.0 mL kg–¹ in seven, eight and eight hemiabdomens, respectively) was injected at the midpoint between the iliac crest and the last rib at the height of the shoulder. In group G0.3×2 (seven hemiabdomens), two injections (0.3 mL kg–¹) were performed, caudal to the last rib and cranial to the iliac crest at the same height. Total time for injection was recorded; after 30 minutes, cadavers were dissected and spread of dye was evaluated.Accuracy of injection site was 80% and injection time was 71 (48–120) seconds for all groups together. Craniocaudal spread was 6.4 ± 1.6, 9.1 ± 2.6, 11.4 ± 2.3 and 11.2 ± 3.8 cm for G0.3, G0.6, G1.0 and G0.3×2, respectively [G0.3 to G0.3×2 (p = 0.044) and G1.0 (p = 0.034)]. There was no difference in dorsoventral spread among groups. Number of ventral nerve branches stained was 3 (2–4), 3 (2–4), 3 (3–4) and 3 (2–4) for G0.3, G0.6, G1.0 and G0.3×2, respectively, including nerve branches from twelfth thoracic to third lumbar (L3) in different proportions among groups.Results suggest that a single-injection TAP block, using 0.3 mL kg–¹, stains comparable number of nerve branches as higher volumes or two-point injection. Despite the volume or technique, consistent staining of the innervation of the caudal abdomen (L1–L3) was observed. Additional cadaveric studies are necessary to identify the optimal technique for complete abdominal wall staining.
Keywords	abdomen ; adults ; analgesia ; anesthesia ; cadaver ; dogs ; dyes ; height ; ilium ; injection site ; innervation ; nerve tissue ; staining ; ultrasonography ; volume
Language	English
Dates of publication	2021-01
Size	p. 142-146.
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-light
ZDB-ID	2020582-X
ISSN	1467-2995 ; 1467-2987
ISSN (online)	1467-2995
ISSN	1467-2987
DOI	10.1016/j.vaa.2020.10.001
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Smartphone otoscope: an alternative technique for intubation in rabbits.

Freitag, Flavio A V / Muehlbauer, Eloisa / Martini, Rafaella / Froes, Tilde R / Duque, Juan C M

Veterinary anaesthesia and analgesia

2019 Volume 47, Issue 2, Page(s) 281–284

MeSH term(s)	Anesthesia/methods ; Anesthesia/veterinary ; Anesthesiology/instrumentation ; Anesthesiology/methods ; Animals ; Intubation, Intratracheal/instrumentation ; Intubation, Intratracheal/methods ; Intubation, Intratracheal/veterinary ; Otoscopes ; Rabbits ; Smartphone
Language	English
Publishing date	2019-11-28
Publishing country	United States
Document type	Letter
ZDB-ID	2020582-X
ISSN	1467-2995 ; 1467-2987
ISSN (online)	1467-2995
ISSN	1467-2987
DOI	10.1016/j.vaa.2019.11.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Evaluation of injection volumes for the transversus abdominis plane block in dog cadavers: a preliminary trial.

Freitag, Flavio A V / Muehlbauer, Eloisa / Gaio, Thalise daS / Dos Santos, Amanda A M / Machado, Marcello / Sanchez, Andrea / Duque, Juan C M

Veterinary anaesthesia and analgesia

2020 Volume 48, Issue 1, Page(s) 142–146

Abstract: Objective: To evaluate staining of nerve branches after the injection of different volumes during ultrasound-guided transversus abdominis plane (TAP) block in dog cadavers.: Study design: Prospective randomized study.: Animals: A total of 15 ... ...

Abstract	Objective: To evaluate staining of nerve branches after the injection of different volumes during ultrasound-guided transversus abdominis plane (TAP) block in dog cadavers. Study design: Prospective randomized study. Animals: A total of 15 frozen/thawed adult dog cadavers. Methods: Hemiabdomens were randomly allocated to one of four groups. In groups G0.3, G0.6 and G1.0, ropivacaine-methylene blue solution (0.3, 0.6 and 1.0 mL kg Results: Accuracy of injection site was 80% and injection time was 71 (48-120) seconds for all groups together. Craniocaudal spread was 6.4 ± 1.6, 9.1 ± 2.6, 11.4 ± 2.3 and 11.2 ± 3.8 cm for G0.3, G0.6, G1.0 and G0.3×2, respectively [G0.3 to G0.3×2 (p = 0.044) and G1.0 (p = 0.034)]. There was no difference in dorsoventral spread among groups. Number of ventral nerve branches stained was 3 (2-4), 3 (2-4), 3 (3-4) and 3 (2-4) for G0.3, G0.6, G1.0 and G0.3×2, respectively, including nerve branches from twelfth thoracic to third lumbar (L3) in different proportions among groups. Conclusions and clinical relevance: Results suggest that a single-injection TAP block, using 0.3 mL kg
MeSH term(s)	Abdominal Muscles ; Animals ; Cadaver ; Dog Diseases ; Dogs ; Nerve Block/veterinary ; Prospective Studies ; Ropivacaine ; Ultrasonography, Interventional/veterinary
Chemical Substances	Ropivacaine (7IO5LYA57N)
Language	English
Publishing date	2020-10-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2020582-X
ISSN	1467-2995 ; 1467-2987
ISSN (online)	1467-2995
ISSN	1467-2987
DOI	10.1016/j.vaa.2020.10.001
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Article ; Online: Novel Anaplasmataceae agents Candidatus Ehrlichia hydrochoerus and Anaplasma spp. Infecting Capybaras, Brazil.

Vieira, Thállitha S W J / Collere, Flávia C M / Ferrari, Larissa D R / Baggio, Rafael A / Lange, Rogério R / Ferrari, Marcos V / Duque, Juan C M / Sanches, Gustavo S / Pereira, Nathália A / Aguiar, Daniel M / Labruna, Marcelo B / Vieira, Rafael F C

Emerging infectious diseases

2022 Volume 28, Issue 2, Page(s) 480–482

Abstract: We amplified Ehrlichia and Anaplasma DNA from Amblyomma dubitatum tick-infested capybaras (Hydrochoerus hydrochaeris) in southern Brazil. Sequencing of 16S rRNA, sodB, and groEL indicated a novel Ehrlichia species, and sequencing of 16S rRNA from 2 ... ...

Abstract	We amplified Ehrlichia and Anaplasma DNA from Amblyomma dubitatum tick-infested capybaras (Hydrochoerus hydrochaeris) in southern Brazil. Sequencing of 16S rRNA, sodB, and groEL indicated a novel Ehrlichia species, and sequencing of 16S rRNA from 2 capybaras indicated a novel Anaplasma species. The tick vectors remain unknown.
MeSH term(s)	Anaplasma/genetics ; Anaplasmataceae/genetics ; Animals ; Brazil/epidemiology ; Ehrlichia/genetics ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Rodentia
Chemical Substances	RNA, Ribosomal, 16S
Language	English
Publishing date	2022-02-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2802.210705
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Article ; Online: 'Candidatus Mycoplasma haematohydrochoerus', a novel hemoplasma species in capybaras (Hydrochoerus hydrochaeris) from Brazil.

Vieira, Rafael F C / Santos, Nelson J R / Valente, Jessica D M / Santos, Leonardo P / Lange, Rogério R / Duque, Juan C M / Ferrari, Marcos V / Barros Filho, Ivan R / Collere, Flávia C M / Ferrari, Larissa D R / Gonçalves, Luiz R / Sanches, Gustavo S / André, Marcos R / Vieira, Thállitha S W J

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

2021 Volume 93, Page(s) 104988

Abstract: Three different species of hemoplasmas have been described in rodents, Mycoplasma coccoides, 'Candidatus Mycoplasma haemomuris' and 'Candidatus Mycoplasma haemosphiggurus'. Additionally, potentially novel hemoplasma species have been detected in wild ... ...

Abstract	Three different species of hemoplasmas have been described in rodents, Mycoplasma coccoides, 'Candidatus Mycoplasma haemomuris' and 'Candidatus Mycoplasma haemosphiggurus'. Additionally, potentially novel hemoplasma species have been detected in wild rodents from Brazil, including capybaras (Hydrochoerus hydrochaeris). Capybaras are the largest rodent in the world and are well adapted to live within close proximity to humans, which increases the risk to spread of zoonotic pathogens. Herein, we investigate the occurrence and genetic diversity of hemoplasmas infecting free-ranging capybaras from southern Brazil. Blood samples and ticks from 17 capybaras were collected. Packed cell volume and total plasma protein were measured, DNA was extracted, and further screened by species-specific and pan-hemoplasma PCR assays targeting the 16S rRNA gene of hemoplasmas. Sixteen out of 17 (94.12%; 95% CI: 73.02-98.95%) were anemic. Only one young female was hypoproteinemic. All capybaras were infested by adults and nymphs of Amblyomma dubitatum ticks. Using the PCR assay targeting the 16S rRNA gene of M. coccoides, 13/17 (76.47%; 95% CI: 52.74-90.44%) capybaras were positive for hemoplasmas. When DNA samples were tested by the pan-hemoplasma PCR, 16/17 (94.12%; 95% CI: 73.02-98.95%) animals were positive. One out of 11 (9.09%) adult ticks salivary glands tested positive for hemoplasma by the pan-hemoplasma PCR assay. Sequencing and phylogenetic analysis of the 16S and 23S rRNA gene fragments confirmed that animals were infected by a novel hemotropic Mycoplasma sp. previously reported in capybaras from Brazil. Additionally, sequencing and phylogenetic analysis of the 23S rRNA gene from three hemoplasma-positive capybaras samples from a previous study performed in midwestern Brazil also confirm our findings. Based on phylogenetic and Neighbor-Net network analysis of the 16S rRNA and 23S rRNA genes, the name 'Candidatus Mycoplasma haematohydrochoerus' is proposed for this novel organism.
MeSH term(s)	Amblyomma/parasitology ; Animals ; Brazil/epidemiology ; Female ; Male ; Mycoplasma/classification ; Mycoplasma Infections/epidemiology ; Mycoplasma Infections/parasitology ; Mycoplasma Infections/veterinary ; Prevalence ; RNA, Protozoan/analysis ; RNA, Ribosomal, 16S/analysis ; RNA, Ribosomal, 23S/analysis ; Rodentia
Chemical Substances	RNA, Protozoan ; RNA, Ribosomal, 16S ; RNA, Ribosomal, 23S
Language	English
Publishing date	2021-06-30
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2037068-4
ISSN	1567-7257 ; 1567-1348
ISSN (online)	1567-7257
ISSN	1567-1348
DOI	10.1016/j.meegid.2021.104988
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Article: ‘Candidatus Mycoplasma haematohydrochoerus’, a novel hemoplasma species in capybaras (Hydrochoerus hydrochaeris) from Brazil

Vieira, Rafael F.C. / Santos, Nelson J.R. / Valente, Jessica D.M. / Santos, Leonardo P. / Lange, Rogério R. / Duque, Juan C.M. / Ferrari, Marcos V. / Barros Filho, Ivan R. / Collere, Flávia C.M. / Ferrari, Larissa D.R. / Gonçalves, Luiz R. / Sanches, Gustavo S. / André, Marcos R. / Vieira, Thállitha S.W.J.

Infection, genetics, and evolution. 2021 Sept., v. 93

2021

Abstract: Three different species of hemoplasmas have been described in rodents, Mycoplasma coccoides, ‘Candidatus Mycoplasma haemomuris’ and ‘Candidatus Mycoplasma haemosphiggurus’. Additionally, potentially novel hemoplasma species have been detected in wild ... ...

Abstract	Three different species of hemoplasmas have been described in rodents, Mycoplasma coccoides, ‘Candidatus Mycoplasma haemomuris’ and ‘Candidatus Mycoplasma haemosphiggurus’. Additionally, potentially novel hemoplasma species have been detected in wild rodents from Brazil, including capybaras (Hydrochoerus hydrochaeris). Capybaras are the largest rodent in the world and are well adapted to live within close proximity to humans, which increases the risk to spread of zoonotic pathogens. Herein, we investigate the occurrence and genetic diversity of hemoplasmas infecting free-ranging capybaras from southern Brazil. Blood samples and ticks from 17 capybaras were collected. Packed cell volume and total plasma protein were measured, DNA was extracted, and further screened by species-specific and pan-hemoplasma PCR assays targeting the 16S rRNA gene of hemoplasmas. Sixteen out of 17 (94.12%; 95% CI: 73.02–98.95%) were anemic. Only one young female was hypoproteinemic. All capybaras were infested by adults and nymphs of Amblyomma dubitatum ticks. Using the PCR assay targeting the 16S rRNA gene of M. coccoides, 13/17 (76.47%; 95% CI: 52.74–90.44%) capybaras were positive for hemoplasmas. When DNA samples were tested by the pan-hemoplasma PCR, 16/17 (94.12%; 95% CI: 73.02–98.95%) animals were positive. One out of 11 (9.09%) adult ticks salivary glands tested positive for hemoplasma by the pan-hemoplasma PCR assay. Sequencing and phylogenetic analysis of the 16S and 23S rRNA gene fragments confirmed that animals were infected by a novel hemotropic Mycoplasma sp. previously reported in capybaras from Brazil. Additionally, sequencing and phylogenetic analysis of the 23S rRNA gene from three hemoplasma-positive capybaras samples from a previous study performed in midwestern Brazil also confirm our findings. Based on phylogenetic and Neighbor-Net network analysis of the 16S rRNA and 23S rRNA genes, the name ‘Candidatus Mycoplasma haematohydrochoerus’ is proposed for this novel organism.
Keywords	Amblyomma ; Hydrochoerus hydrochaeris ; Mycoplasma haemomuris ; adults ; anemia ; blood proteins ; females ; genes ; genetic variation ; hematocrit ; infection ; phylogeny ; risk ; rodents ; Brazil
Language	English
Dates of publication	2021-09
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2037068-4
ISSN	1567-1348
ISSN	1567-1348
DOI	10.1016/j.meegid.2021.104988
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