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Article: Humoral anti-SARS-CoV-2 immune response after two doses of Comirnaty vaccine in nursing home residents by previous infection status

Helle, François / Moyet, Julien / Demey, Baptiste / François, Catherine / Duverlie, Gilles / Castelain, Sandrine / Bloch, Fréderic / Brochot, Etienne

Vaccine. 2022 Jan. 24, v. 40, no. 3

2022

Abstract: Coronavirus disease 2019 (COVID-19) is associated with increased morbidity and mortality in older adults. Although the advent of the first vaccines has significantly reduced these rates, data on older adults in clinical trials are scarce.We quantified ... ...

Abstract	Coronavirus disease 2019 (COVID-19) is associated with increased morbidity and mortality in older adults. Although the advent of the first vaccines has significantly reduced these rates, data on older adults in clinical trials are scarce.We quantified and compared the humoral response in individuals with vs. without pre-existing seropositivity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in a cohort of 69 patients living in a nursing home and who had received the recommended two doses of the Comirnaty (Pfizer-BioNTech®) vaccine.All 69 patients (100%) tested positive for antibodies against SARS-CoV-2 at 2 months post-vaccination. Residents with a pre-vaccination infection had significantly higher titers of anti-spike 1 IgG than those with no prior infection (median [interquartile range]: 55,726 [14463–78852] vs. 1314 [272–1249] arbitrary units, respectively; p < 0.001). The same result was observed for neutralizing antibodies titers (704 [320–1280] vs. 47 [20–40] respectively; p < 0.001). Between the pre-vaccination and post-vaccination periods, for IgG and neutralizing antibodies, we observed a 49 and 8-fold increase respectively. In comparison to the wild-type Receptor Binding Domain (RBD), the binding capacity of these vaccine sera was significantly decreased on the B.1.351 and P.1 variants RBD but not decreased with respect to the B.1.1.7 RBD.Although all nursing home residents developed a humoral response following Comirnaty vaccine, its intensity appeared to depend on the pre-vaccination serological status.Our results raise the question of how many doses of vaccine should be administered in older and how long the protection will be effective.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; humoral immunity ; immune response ; morbidity ; mortality ; seroprevalence ; vaccines
Language	English
Dates of publication	2022-0124
Size	p. 531-535.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2021.11.086
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Intercellular Transmission of Naked Viruses through Extracellular Vesicles: Focus on Polyomaviruses.

Helle, Francois / Handala, Lynda / Bentz, Marine / Duverlie, Gilles / Brochot, Etienne

Viruses

2020 Volume 12, Issue 10

Abstract: Extracellular vesicles have recently emerged as a novel mode of viral transmission exploited by naked viruses to exit host cells through a nonlytic pathway. Extracellular vesicles can allow multiple viral particles to collectively traffic in and out of ... ...

Abstract	Extracellular vesicles have recently emerged as a novel mode of viral transmission exploited by naked viruses to exit host cells through a nonlytic pathway. Extracellular vesicles can allow multiple viral particles to collectively traffic in and out of cells, thus enhancing the viral fitness and diversifying the transmission routes while evading the immune system. This has been shown for several RNA viruses that belong to the Picornaviridae, Hepeviridae, Reoviridae, and Caliciviridae families; however, recent studies also demonstrated that the BK and JC viruses, two DNA viruses that belong to the Polyomaviridae family, use a similar strategy. In this review, we provide an update on recent advances in understanding the mechanisms used by naked viruses to hijack extracellular vesicles, and we discuss the implications for the biology of polyomaviruses.
MeSH term(s)	Antibodies, Neutralizing/immunology ; Antibodies, Viral/immunology ; Endocytosis/physiology ; Extracellular Vesicles/virology ; Humans ; Immune Evasion/physiology ; Polyomavirus/immunology ; Polyomavirus/metabolism ; Polyomavirus Infections/transmission ; Tumor Virus Infections/transmission ; Tumor Virus Infections/virology ; Virus Internalization ; Virus Replication/physiology
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2020-09-26
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v12101086
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis.

Aubry, Aurélien / Demey, Baptiste / François, Catherine / Duverlie, Gilles / Castelain, Sandrine / Helle, François / Brochot, Etienne

Journal of clinical medicine

2021 Volume 10, Issue 9

Abstract: Background: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce.: Methods: We thus longitudinally evaluated and compared the total and neutralizing immune response of ...

Abstract	Background: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. Methods: We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT-PCR using several commercial assays. Results: Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays ( Conclusions: In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results.
Language	English
Publishing date	2021-04-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm10091815
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Article: Intercellular Transmission of Naked Viruses through Extracellular Vesicles: Focus on Polyomaviruses

Helle, Francois / Handala, Lynda / Bentz, Marine / Duverlie, Gilles / Brochot, Etienne

Viruses. 2020 Sept. 26, v. 12, no. 10

2020

Abstract	Extracellular vesicles have recently emerged as a novel mode of viral transmission exploited by naked viruses to exit host cells through a nonlytic pathway. Extracellular vesicles can allow multiple viral particles to collectively traffic in and out of cells, thus enhancing the viral fitness and diversifying the transmission routes while evading the immune system. This has been shown for several RNA viruses that belong to the Picornaviridae, Hepeviridae, Reoviridae, and Caliciviridae families; however, recent studies also demonstrated that the BK and JC viruses, two DNA viruses that belong to the Polyomaviridae family, use a similar strategy. In this review, we provide an update on recent advances in understanding the mechanisms used by naked viruses to hijack extracellular vesicles, and we discuss the implications for the biology of polyomaviruses.
Keywords	Caliciviridae ; Hepeviridae ; Picornaviridae ; Polyomaviridae ; Reoviridae ; immune system ; virion ; virus transmission ; viruses
Language	English
Dates of publication	2020-0926
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v12101086
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Comparison of different serological assays for SARS-CoV-2 in real life.

Brochot, Etienne / Demey, Baptiste / Handala, Lynda / François, Catherine / Duverlie, Gilles / Castelain, Sandrine

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

2020 Volume 130, Page(s) 104569

Abstract: Background: The emergence of the global SARS-CoV-2 pandemic required the rapid and large-scale deployment of PCR and serological tests in different formats.: Objectives: Real-life evaluation of these tests is needed. Using 168 samples from patients ... ...

Abstract	Background: The emergence of the global SARS-CoV-2 pandemic required the rapid and large-scale deployment of PCR and serological tests in different formats. Objectives: Real-life evaluation of these tests is needed. Using 168 samples from patients hospitalized for COVID-19, non-hospitalized patients but infected with SARS-CoV-2, patients participating in screening campaigns, and samples from patients with a history of other seasonal coronavirus infections, we evaluated the clinical performance of 5 serological assays widely used worldwide (WANTAI®, BIORAD®, EUROIMMUN®, ABBOTT® and LIAISON®). Results: For hospitalized patients, all these assays showed a sensitivity of 100 % from day 9 after the symptoms onset. On the other hand, sensitivity was much lower for patients who did not require hospitalization for COVID-19 confirmed by PCR (from 91.6 % for WANTAI® to 69 % for LIAISON®). These differences do not seem to be due to the antigens chosen by the manufacturers but more to the test formats (IgG detection versus total antibodies). In addition, more than 50 days after a positive PCR for CoV-2-SARS the proportion of positive patients seem to decrease. We did not observe any significant cross-reactions for these techniques with the four other seasonal coronaviruses. Conclusion: In conclusion, the evaluation and knowledge of the serological tests used is important and should require an optimized strategy adaptation of the analysis laboratories to best meet patient's expectations in the face of this health crisis.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Viral/blood ; Betacoronavirus ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/immunology ; Female ; Hospitalization/statistics & numerical data ; Humans ; Immunoglobulin A/blood ; Immunoglobulin G/blood ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/immunology ; Reagent Kits, Diagnostic/standards ; SARS-CoV-2 ; Sensitivity and Specificity ; Serologic Tests/methods ; Young Adult
Chemical Substances	Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin G ; Reagent Kits, Diagnostic
Keywords	covid19
Language	English
Publishing date	2020-08-02
Publishing country	Netherlands
Document type	Comparative Study ; Journal Article
ZDB-ID	1446080-4
ISSN	1873-5967 ; 1386-6532
ISSN (online)	1873-5967
ISSN	1386-6532
DOI	10.1016/j.jcv.2020.104569
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Zs.A 3790: Show issues

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Article ; Online: BK Polyomavirus Micro-RNAs: Time Course and Clinical Relevance in Kidney Transplant Recipients.

Demey, Baptiste / Descamps, Véronique / Presne, Claire / Helle, Francois / Francois, Catherine / Duverlie, Gilles / Castelain, Sandrine / Brochot, Etienne

Viruses

2021 Volume 13, Issue 2

Abstract: Background: Kidney transplant recipients (KTRs) are exposed to a high risk of BK polyomavirus (BKPyV) replication, which in turn may lead to graft loss. Although the microRNAs (miRNAs) bkv-miR-B1-3p and bkv-miR-B1-5p are produced during the viral cycle, ...

Abstract	Background: Kidney transplant recipients (KTRs) are exposed to a high risk of BK polyomavirus (BKPyV) replication, which in turn may lead to graft loss. Although the microRNAs (miRNAs) bkv-miR-B1-3p and bkv-miR-B1-5p are produced during the viral cycle, their putative value as markers of viral replication has yet to be established. In KTRs, the clinical relevance of the changes over time in BKPyV miRNA levels has not been determined. Methods: In a retrospective study, we analyzed 186 urine samples and 120 plasma samples collected from 67 KTRs during the first year post-transplantation. Using a reproducible, standardized, quantitative RT-PCR assay, we measured the levels of bkv-miR-B1-3p and bkv-miR-B1-5p (relative to the BKPyV DNA load). Results: Detection of the two miRNAs had low diagnostic value for identifying patients with DNAemia or for predicting DNAuria during follow-up. Seven of the 14 KTRs with a sustained BKPyV infection within the first year post-transplantation showed a progressive reduction in the DNA load and then a rapid disappearance of the miRNAs. DNA and miRNA loads were stable in the other seven KTRs. Conclusions: After the DNA-based diagnosis of BKPyV infection in KTRs, bkv-miR-B1-3p and bkv-miR-B1-5p levels in the urine might be valuable markers for viral replication monitoring and thus might help physicians to avoid an excessive reduction in the immunosuppressive regimen.
MeSH term(s)	Adult ; Aged ; BK Virus/genetics ; BK Virus/physiology ; Biomarkers/blood ; Biomarkers/urine ; Female ; Humans ; Kidney Transplantation/adverse effects ; Kidney Transplantation/statistics & numerical data ; Male ; MicroRNAs/blood ; MicroRNAs/genetics ; MicroRNAs/urine ; Middle Aged ; Polyomavirus Infections/blood ; Polyomavirus Infections/etiology ; Polyomavirus Infections/urine ; Polyomavirus Infections/virology ; Postoperative Complications/blood ; Postoperative Complications/etiology ; Postoperative Complications/urine ; Postoperative Complications/virology ; RNA, Viral/blood ; RNA, Viral/genetics ; RNA, Viral/urine ; Retrospective Studies ; Transplant Recipients/statistics & numerical data ; Viral Load ; Virus Replication
Chemical Substances	Biomarkers ; MicroRNAs ; RNA, Viral
Language	English
Publishing date	2021-02-23
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13020351
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Comparison of nasopharyngeal and saliva swabs for the detection of RNA SARS-CoV-2 during mass screening (SALICOV study).

Castelain, Sandrine / François, Catherine / Demey, Baptiste / Aubry, Aurelien / Lanoix, Jean-Philippe / Duverlie, Gilles / Schmit, Jean-Luc / Brochot, Etienne

The new microbiologica

2021 Volume 44, Issue 1, Page(s) 59–61

Abstract: In the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes ...

Abstract	In the context of a second wave of SARS-CoV-2 transmission, the use of saliva sampling has become an issue of real importance. SARS-CoV-2 RNA screening was performed on nasopharyngeal and saliva swabs collected from 501 individuals from residential homes for the elderly. The saliva samples were collected at the same time as the nasopharyngeal samples. Nasopharyngeal samples yielded positive results for 26 individuals, only two of whom also tested positive with saliva swabs. In this context, saliva collected by swabbing the fluid is not an ideal sample.
MeSH term(s)	Aged ; COVID-19 ; Humans ; Mass Screening ; RNA, Viral/genetics ; SARS-CoV-2 ; Saliva
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-02-14
Publishing country	Italy
Document type	Journal Article
ZDB-ID	756168-4
ISSN	1121-7138 ; 0391-5352
ISSN	1121-7138 ; 0391-5352
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Article ; Online: Humoral anti-SARS-CoV-2 immune response after two doses of Comirnaty vaccine in nursing home residents by previous infection status.

Helle, François / Moyet, Julien / Demey, Baptiste / François, Catherine / Duverlie, Gilles / Castelain, Sandrine / Bloch, Fréderic / Brochot, Etienne

Vaccine

2021 Volume 40, Issue 3, Page(s) 531–535

Abstract: Background: Coronavirus disease 2019 (COVID-19) is associated with increased morbidity and mortality in older adults. Although the advent of the first vaccines has significantly reduced these rates, data on older adults in clinical trials are scarce.: ...

Abstract	Background: Coronavirus disease 2019 (COVID-19) is associated with increased morbidity and mortality in older adults. Although the advent of the first vaccines has significantly reduced these rates, data on older adults in clinical trials are scarce. Objectives: We quantified and compared the humoral response in individuals with vs. without pre-existing seropositivity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in a cohort of 69 patients living in a nursing home and who had received the recommended two doses of the Comirnaty (Pfizer-BioNTech®) vaccine. Results: All 69 patients (100%) tested positive for antibodies against SARS-CoV-2 at 2 months post-vaccination. Residents with a pre-vaccination infection had significantly higher titers of anti-spike 1 IgG than those with no prior infection (median [interquartile range]: 55,726 [14463-78852] vs. 1314 [272-1249] arbitrary units, respectively; p < 0.001). The same result was observed for neutralizing antibodies titers (704 [320-1280] vs. 47 [20-40] respectively; p < 0.001). Between the pre-vaccination and post-vaccination periods, for IgG and neutralizing antibodies, we observed a 49 and 8-fold increase respectively. In comparison to the wild-type Receptor Binding Domain (RBD), the binding capacity of these vaccine sera was significantly decreased on the B.1.351 and P.1 variants RBD but not decreased with respect to the B.1.1.7 RBD. Although all nursing home residents developed a humoral response following Comirnaty vaccine, its intensity appeared to depend on the pre-vaccination serological status. Conclusion: Our results raise the question of how many doses of vaccine should be administered in older and how long the protection will be effective.
MeSH term(s)	Aged ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; COVID-19 Vaccines ; Humans ; Immunity, Humoral ; Nursing Homes ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Vaccines
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Spike Glycoprotein, Coronavirus ; Vaccines
Language	English
Publishing date	2021-12-03
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2021.11.086
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Article: Correction to: The expression of HCV-associated host factors is dependent on the hepatoma cell line used in HCV studies

Hoffmann, Thomas Walter / Castelain, Sandrine / Delfosse, Fabien / Duverlie, Gilles / François, Catherine / Helle, François

Archives of virology. 2018 Dec., v. 163, no. 12

2018

Abstract: In this article, a clone of HepG2 stably expressing CD81 (HepG2-CD81) was used. Unfortunately, after cell line authentication. ...

Abstract	In this article, a clone of HepG2 stably expressing CD81 (HepG2-CD81) was used. Unfortunately, after cell line authentication.
Keywords	CD antigens ; cell lines ; Hepatitis C virus ; hepatoma
Language	English
Dates of publication	2018-12
Size	p. 3485.
Publishing place	Springer Vienna
Document type	Article
Note	Published Erratum
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-018-4044-y
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Article ; Online: Does Therapeutic Drug Monitoring of Ribavirin in HCV Genotype 3 Treatment With Sofosbuvir and Ribavirin Still Have a Role?

Brochot, Etienne / Bodeau, Sandra / Duverlie, Gilles

Therapeutic drug monitoring

2015 Volume 37, Issue 4, Page(s) 550–551

MeSH term(s)	Antiviral Agents/blood ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Drug Monitoring ; Drug Therapy, Combination ; Genotype ; Hepacivirus/drug effects ; Hepacivirus/genetics ; Hepatitis C, Chronic/blood ; Hepatitis C, Chronic/drug therapy ; Hepatitis C, Chronic/virology ; Humans ; Ribavirin/blood ; Ribavirin/pharmacology ; Ribavirin/therapeutic use ; Sofosbuvir/pharmacology ; Sofosbuvir/therapeutic use
Chemical Substances	Antiviral Agents ; Ribavirin (49717AWG6K) ; Sofosbuvir (WJ6CA3ZU8B)
Language	English
Publishing date	2015-08
Publishing country	United States
Document type	Letter
ZDB-ID	424443-6
ISSN	1536-3694 ; 0163-4356
ISSN (online)	1536-3694
ISSN	0163-4356
DOI	10.1097/FTD.0000000000000161
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