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  1. AU="Dziuba, Marina V"
  2. AU="Aleksander Zińczuk"
  3. AU="Rowe, Logan M."
  4. AU="Sharma N." AU="Sharma N."
  5. AU=Yuan Shu
  6. AU="Ye Liu"
  7. AU="Bezerra, Antônio Diego M"
  8. AU="HE Xiufeng"
  9. AU=Freeman Hugh J AU=Freeman Hugh J
  10. AU="Choi, John Kim"
  11. AU="Streng, Bianca M M"
  12. AU="Franklin, Renty B"
  13. AU="Tetri, Laura H"
  14. AU="Badve, Sunil V"
  15. AU=Zhang Yinan
  16. AU="Piquero, Nicole Leeper"
  17. AU="Russo, Giorgio Ivan" AU="Russo, Giorgio Ivan"
  18. AU=Pourdowlat Guitti
  19. AU="Frisenda, Riccardo"
  20. AU=Palmucci Stefano

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  1. Artikel ; Online: Exploring the host range for genetic transfer of magnetic organelle biosynthesis.

    Dziuba, Marina V / Müller, Frank-Dietrich / Pósfai, Mihály / Schüler, Dirk

    Nature nanotechnology

    2023  Band 19, Heft 1, Seite(n) 115–123

    Abstract: Magnetosomes produced by magnetotactic bacteria have great potential for application in biotechnology and medicine due to their unique physicochemical properties and high biocompatibility. Attempts to transfer the genes for magnetosome biosynthesis into ... ...

    Abstract Magnetosomes produced by magnetotactic bacteria have great potential for application in biotechnology and medicine due to their unique physicochemical properties and high biocompatibility. Attempts to transfer the genes for magnetosome biosynthesis into non-magnetic organisms have had mixed results. Here we report on a systematic study to identify key components needed for magnetosome biosynthesis after gene transfer. We transfer magnetosome genes to 25 proteobacterial hosts, generating seven new magnetosome-producing strains. We characterize the recombinant magnetosomes produced by these strains and demonstrate that denitrification and anaerobic photosynthesis are linked to the ability to synthesize magnetosomes upon the gene transfer. In addition, we show that the number of magnetosomes synthesized by a foreign host negatively correlates with the guanine-cytosine content difference between the host and the gene donor. Our findings have profound implications for the generation of magnetized living cells and the potential for transgenic biogenic magnetic nanoparticle production.
    Mesh-Begriff(e) Magnetospirillum/genetics ; Magnetosomes/genetics ; Magnetosomes/chemistry ; Biotechnology ; Magnetic Phenomena ; Host Specificity ; Bacterial Proteins
    Chemische Substanzen Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-09-21
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2254964-X
    ISSN 1748-3395 ; 1748-3387
    ISSN (online) 1748-3395
    ISSN 1748-3387
    DOI 10.1038/s41565-023-01500-5
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Single-step transfer of biosynthetic operons endows a non-magnetotactic Magnetospirillum strain from wetland with magnetosome biosynthesis.

    Dziuba, Marina V / Zwiener, Theresa / Uebe, Rene / Schüler, Dirk

    Environmental microbiology

    2020  Band 22, Heft 4, Seite(n) 1603–1618

    Abstract: The magnetotactic lifestyle represents one of the most complex traits found in many bacteria from aquatic environments and depends on magnetic organelles, the magnetosomes. Genetic transfer of magnetosome biosynthesis operons to a non-magnetotactic ... ...

    Abstract The magnetotactic lifestyle represents one of the most complex traits found in many bacteria from aquatic environments and depends on magnetic organelles, the magnetosomes. Genetic transfer of magnetosome biosynthesis operons to a non-magnetotactic bacterium has only been reported once so far, but it is unclear whether this may also occur in other recipients. Besides magnetotactic species from freshwater, the genus Magnetospirillum of the Alphaproteobacteria also comprises a number of strains lacking magnetosomes, which are abundant in diverse microbial communities. Their close phylogenetic interrelationships raise the question whether the non-magnetotactic magnetospirilla may have the potential to (re)gain a magnetotactic lifestyle upon acquisition of magnetosome gene clusters. Here, we studied the transfer of magnetosome gene operons into several non-magnetotactic environmental magnetospirilla. Single-step transfer of a compact vector harbouring >30 major magnetosome genes from M. gryphiswaldense induced magnetosome biosynthesis in a Magnetospirillum strain from a constructed wetland. However, the resulting magnetic cellular alignment was insufficient for efficient magnetotaxis under conditions mimicking the weak geomagnetic field. Our work provides insights into possible evolutionary scenarios and potential limitations for the dissemination of magnetotaxis by horizontal gene transfer and expands the range of foreign recipients that can be genetically magnetized.
    Mesh-Begriff(e) Gene Transfer, Horizontal ; Magnetosomes/metabolism ; Magnetospirillum/genetics ; Magnetospirillum/metabolism ; Multigene Family ; Operon ; Phylogeny ; Wetlands
    Sprache Englisch
    Erscheinungsdatum 2020-03-09
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2020213-1
    ISSN 1462-2920 ; 1462-2912
    ISSN (online) 1462-2920
    ISSN 1462-2912
    DOI 10.1111/1462-2920.14950
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Single‐step transfer of biosynthetic operons endows a non‐magnetotactic Magnetospirillum strain from wetland with magnetosome biosynthesis

    Dziuba, Marina V / Zwiener, Theresa / Uebe, Rene / Schüler, Dirk

    Environmental microbiology. 2020 Apr., v. 22, no. 4

    2020  

    Abstract: The magnetotactic lifestyle represents one of the most complex traits found in many bacteria from aquatic environments and depends on magnetic organelles, the magnetosomes. Genetic transfer of magnetosome biosynthesis operons to a non‐magnetotactic ... ...

    Abstract The magnetotactic lifestyle represents one of the most complex traits found in many bacteria from aquatic environments and depends on magnetic organelles, the magnetosomes. Genetic transfer of magnetosome biosynthesis operons to a non‐magnetotactic bacterium has only been reported once so far, but it is unclear whether this may also occur in other recipients. Besides magnetotactic species from freshwater, the genus Magnetospirillum of the Alphaproteobacteria also comprises a number of strains lacking magnetosomes, which are abundant in diverse microbial communities. Their close phylogenetic interrelationships raise the question whether the non‐magnetotactic magnetospirilla may have the potential to (re)gain a magnetotactic lifestyle upon acquisition of magnetosome gene clusters. Here, we studied the transfer of magnetosome gene operons into several non‐magnetotactic environmental magnetospirilla. Single‐step transfer of a compact vector harbouring >30 major magnetosome genes from M. gryphiswaldense induced magnetosome biosynthesis in a Magnetospirillum strain from a constructed wetland. However, the resulting magnetic cellular alignment was insufficient for efficient magnetotaxis under conditions mimicking the weak geomagnetic field. Our work provides insights into possible evolutionary scenarios and potential limitations for the dissemination of magnetotaxis by horizontal gene transfer and expands the range of foreign recipients that can be genetically magnetized.
    Schlagwörter Magnetospirillum gryphiswaldense ; aquatic environment ; bacteria ; biosynthesis ; constructed wetlands ; freshwater ; geophysics ; horizontal gene transfer ; lifestyle ; magnetic fields ; magnetotaxis ; microbial communities ; multigene family ; operon ; organelles ; phylogeny
    Sprache Englisch
    Erscheinungsverlauf 2020-04
    Umfang p. 1603-1618.
    Erscheinungsort John Wiley & Sons, Inc.
    Dokumenttyp Artikel
    Anmerkung JOURNAL ARTICLE
    ZDB-ID 2020213-1
    ISSN 1462-2920 ; 1462-2912
    ISSN (online) 1462-2920
    ISSN 1462-2912
    DOI 10.1111/1462-2920.14950
    Datenquelle NAL Katalog (AGRICOLA)

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  4. Artikel ; Online: Ecology and biogeography of the 'marine Geitlerinema' cluster and a description of Sodalinema orleanskyi sp. nov., Sodalinema gerasimenkoae sp. nov., Sodalinema stali sp. nov. and Baaleninema simplex gen. et sp. nov. (Oscillatoriales, Cyanobacteria).

    Samylina, Olga S / Sinetova, Maria A / Kupriyanova, Elena V / Starikov, Alexander Yu / Sukhacheva, Marina V / Dziuba, Marina V / Tourova, Tatiana P

    FEMS microbiology ecology

    2021  Band 97, Heft 8

    Abstract: Filamentous cyanobacteria belonging to the 'marine Geitlerinema' cluster are spread worldwide in saline environments and considered to play an important ecological role. However, the taxonomy of this group remains unclear. Here, we analyzed the phylogeny, ...

    Abstract Filamentous cyanobacteria belonging to the 'marine Geitlerinema' cluster are spread worldwide in saline environments and considered to play an important ecological role. However, the taxonomy of this group remains unclear. Here, we analyzed the phylogeny, ecology and biogeography of the 'marine Geitlerinema' cluster representatives and revealed two subclusters: (1) an 'oceanic' subcluster containing PCC7105 clade and black band disease (BBD) clade with free-living and pathogenic strains distributed in Atlantic, Indian and Pacific Ocean-related localities, and (2) a Sodalinema subcluster containing free-living strains from marine, hypersaline, saline-alkaline and soda lake habitats from the Eurasian and African continents. Polyphasic analysis using genetic and phenotypic criteria demonstrated that these two groups represent separate genera. Representatives of Sodalinema subcluster were phylogenetically attributed to the genus Sodalinema. Our data expand the ecological and geographical distribution of this genus. We emended the description of the genus Sodalinema and proposed three new species differing in phylogenetic, geographic and ecological criteria: Sodalinema orleanskyi sp. nov., Sodalinema gerasimenkoae sp. nov. and Sodalinema stali sp. nov. Additionally, a new genus and species Baaleninema simplex gen. et sp. nov. was discribed within the PCC7105 clade. By this, we put in order the current confusion of the 'marine Geitlerinema' group and highlight its ecological diversity.
    Sprache Englisch
    Erscheinungsdatum 2021-07-13
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 283722-5
    ISSN 1574-6941 ; 0168-6496
    ISSN (online) 1574-6941
    ISSN 0168-6496
    DOI 10.1093/femsec/fiab104
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: Draft Genome Sequences of Two Magnetotactic Bacteria, Magnetospirillum moscoviense BB-1 and Magnetospirillum marisnigri SP-1.

    Koziaeva, Veronika V / Dziuba, Marina V / Ivanov, Timophey M / Kuznetsov, Boris B / Skryabin, Konstantin G / Grouzdev, Denis S

    Genome announcements

    2016  Band 4, Heft 4

    Abstract: We report here the draft genome sequences of two recently isolated magnetotactic species, Magnetospirillum moscoviense BB-1 and Magnetospirillum marisnigri SP-1. The genome of M. moscoviense BB-1 has 4,164,497 bp, 65.2% G+C content, and comprises 207 ... ...

    Abstract We report here the draft genome sequences of two recently isolated magnetotactic species, Magnetospirillum moscoviense BB-1 and Magnetospirillum marisnigri SP-1. The genome of M. moscoviense BB-1 has 4,164,497 bp, 65.2% G+C content, and comprises 207 contigs. The genome of M. marisnigri SP-1 consists of 131 contigs and has a length of 4,619,819 bp and 64.7% G+C content.
    Sprache Englisch
    Erscheinungsdatum 2016-08-11
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2704277-7
    ISSN 2169-8287
    ISSN 2169-8287
    DOI 10.1128/genomeA.00814-16
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.

    Grouzdev, Denis S / Dziuba, Marina V / Kurek, Denis V / Ovchinnikov, Alexander I / Zhigalova, Nadezhda A / Kuznetsov, Boris B / Skryabin, Konstantin G

    PloS one

    2014  Band 9, Heft 10, Seite(n) e109914

    Abstract: In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC ...

    Abstract In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from Bacillus subtilis as anchors and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used. With Response Surface Methodology (RSM) the highest level of proteins integration into magnetosome membrane was achieved under the following parameters: pH 8.78, without adding NaCl and 55 s of vortexing for Mbb; pH 9.48, 323 mM NaCl and 55 s of vortexing for Mistbb. Modified magnetosomes with Mbb and Mistbb displayed on their surface demonstrated comparable levels of IgG-binding activity, suggesting that both proteins could be efficiently used as anchor molecules. We also demonstrated that such modified magnetosomes are stable in PBS buffer during at least two weeks. IgG-binding magnetosomes obtained by this approach could serve as a multifunctional platform for displaying various types of antibodies.
    Mesh-Begriff(e) Bacterial Proteins/metabolism ; Immunoglobulin G/metabolism ; Magnetite Nanoparticles/chemistry ; Magnetosomes/chemistry ; Membrane Proteins/metabolism ; Protein Binding ; Staphylococcal Protein A/metabolism ; Staphylococcus aureus/metabolism
    Chemische Substanzen Bacterial Proteins ; Immunoglobulin G ; Magnetite Nanoparticles ; Membrane Proteins ; Staphylococcal Protein A
    Sprache Englisch
    Erscheinungsdatum 2014-10-15
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0109914
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel: Draft Genome Sequence of Magnetospirillum sp. Strain SO-1, a Freshwater Magnetotactic Bacterium Isolated from the Ol'khovka River, Russia.

    Grouzdev, Denis S / Dziuba, Marina V / Sukhacheva, Marina S / Mardanov, Andrey V / Beletskiy, Aleksey V / Kuznetsov, Boris B / Skryabin, Konstantin G

    Genome announcements

    2014  Band 2, Heft 2

    Abstract: Here, we present the draft genome sequence of Magnetospirillum sp. strain SO-1, a freshwater magnetotactic spirillum isolated from the sediments of the Ol'khovka River, Russia. ...

    Abstract Here, we present the draft genome sequence of Magnetospirillum sp. strain SO-1, a freshwater magnetotactic spirillum isolated from the sediments of the Ol'khovka River, Russia.
    Sprache Englisch
    Erscheinungsdatum 2014-04-10
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2704277-7
    ISSN 2169-8287
    ISSN 2169-8287
    DOI 10.1128/genomeA.00235-14
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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