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Article ; Online: Incidental diagnosis of mpox virus infection in patients undergoing sexually transmitted infection screening-findings from a study in France.

Edouard, Sophie / Boschi, Céline / Colson, Philippe / Million, Matthieu / Fournier, Pierre-Edouard / La Scola, Bernard / Fenollar, Florence

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

2024 Volume 143, Page(s) 107009

Abstract: Objectives: This study aimed to investigate the prevalence of mpox virus (MPXV) infections in the general population consulting for routine sexually transmitted infections (STIs) in our Marseille public hospital. In fact, the recent worldwide MPXV ... ...

Abstract	Objectives: This study aimed to investigate the prevalence of mpox virus (MPXV) infections in the general population consulting for routine sexually transmitted infections (STIs) in our Marseille public hospital. In fact, the recent worldwide MPXV outbreak mainly impacted men who have sex with men and the prevalence of MPXV infections in the general population remains poorly defined. Methods: All samples addressed routinely to our microbiological laboratory for STIs between July 1 and October 15, 2022 were screened with MPXV-specific quantitative polymerase chain reaction. Results: A total of 2688 samples from 1896 patients suspected of having STIs were tested and eight (0.4%) patients were incidentally diagnosed with MPXV infection, including six men and two women. MPXV was detected in rectal swabs (n = 2), urine (n = 2), vaginal swabs (n = 2), a urethral swab (n = 1), and a skin swab (n = 1). Conclusions: This study suggests that some MPXV infections are likely to be underdiagnosed because of their non-specific clinical presentation and/or insufficient clinical knowledge of the disease. Our data showed that systematic screening was particularly useful for detecting MPXV in patients without classic lesions or cases of asymptomatic carriage in patients reporting recent high-risk exposure and in patients presenting no obvious risk factor.
MeSH term(s)	Humans ; Male ; Female ; Adult ; France/epidemiology ; Middle Aged ; Prevalence ; Incidental Findings ; Sexually Transmitted Diseases/diagnosis ; Sexually Transmitted Diseases/epidemiology ; Sexually Transmitted Diseases/virology ; Young Adult ; Mass Screening/methods
Language	English
Publishing date	2024-03-21
Publishing country	Canada
Document type	Journal Article
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2024.107009
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Article: Coxiella burnetii

Azouzi, Farah / Olagne, Louis / Edouard, Sophie / Cammilleri, Serge / Magnan, Pierre-Edouard / Fournier, Pierre-Edouard / Million, Matthieu

Microorganisms

2023 Volume 11, Issue 9

Abstract: Cardiovascular infections are the most severe and potentially lethal among the persistent ... ...

Abstract	Cardiovascular infections are the most severe and potentially lethal among the persistent focalized
Language	English
Publishing date	2023-08-24
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11092146
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Article: Protection from successive Omicron variants with SARS-CoV-2 vaccine and monoclonal antibodies in kidney transplant recipients.

Moal, Valérie / Valade, Margaux / Boschi, Céline / Robert, Thomas / Orain, Nicolas / Bancod, Audrey / Edouard, Sophie / Colson, Philippe / La Scola, Bernard

Frontiers in microbiology

2023 Volume 14, Page(s) 1147455

Abstract: Introduction: Kidney transplant recipients (KTRs) are at high risk of severe COVID-19, even when they are fully vaccinated. Additional booster vaccinations or passive immunization with prophylactic monoclonal antibodies are recommended to increase their ...

Abstract	Introduction: Kidney transplant recipients (KTRs) are at high risk of severe COVID-19, even when they are fully vaccinated. Additional booster vaccinations or passive immunization with prophylactic monoclonal antibodies are recommended to increase their protection against severe COVID-19. Methods: Here, we describe the neutralization of SARS-CoV-2 Delta, Omicron BA.1, BA.2, BA.4, and BA.5 variants, firstly by 39 serum samples from vaccinated KTRs exhibiting anti-spike antibody concentrations ≥264 binding antibody units (BAU)/mL and, secondly, by tixagevimab/cilgavimab. Results: No neutralization was observed for 18% of the KTRs, while serum from only 46% of patients could neutralize the five variants. Cross-neutralization of the Delta and Omicron variants occurred for 65-87% of sera samples. The anti-spike antibody concentration correlated with neutralization activity for all the variants. The neutralization titers against the Delta variant were higher in vaccinated KTRs who had previously presented with COVID-19, compared to those KTRs who had only been vaccinated. Breakthrough infections occurred in 39% of the KTRs after the study. Tixagevimab/cilgavimab poorly neutralizes Omicron variants, particularly BA.5, and does not neutralize BQ.1, which is currently the most prevalent strain. Discussion: As a result, sera from seropositive vaccinated KTRs had poor neutralization of the successive Omicron variants. Several Omicron variants are able to escape tixagevimab/cilgavimab.
Language	English
Publishing date	2023-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1147455
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Article: Evidence of antibodies against SARS‐CoV‐2 in wild mustelids from Brittany (France)

Davoust, Bernard / Guérin, Patrick / Orain, Nicolas / Fligny, Camille / Flirden, Fabien / Fenollar, Florence / Mediannikov, Oleg / Edouard, Sophie

Transboundary and emerging diseases. 2022 Sept., v. 69, no. 5

2022

Abstract: In the French region of Brittany, mainly in the department of the Côtes d'Armor, during the first half of 2021, seropositivity for SARS‐CoV‐2 was detected in five wild mustelids out of 33 animals tested (15.6%). Anti‐SARS‐CoV‐2 IgG was detected against ... ...

Abstract	In the French region of Brittany, mainly in the department of the Côtes d'Armor, during the first half of 2021, seropositivity for SARS‐CoV‐2 was detected in five wild mustelids out of 33 animals tested (15.6%). Anti‐SARS‐CoV‐2 IgG was detected against at least four out of five recombinant viral proteins (S1 receptor binding domain, nucleocapsid, S1 subunit, S2 subunit and spike) in three pine martens (Martes martes) and in two badgers (Meles meles) using the automated western blot technique. An ELISA test also identified seropositive cases, although these did not align with western blot results. Although the 171 qPCRs carried out on samples from the 33 mustelids were all negative, these preliminary results from this observational study nevertheless bear witness to infections of unknown origin. The epidemiological surveillance of Covid‐19 in wildlife must continue, in particular with effective serology tools.
Keywords	COVID-19 infection ; Martes martes ; Meles meles ; Severe acute respiratory syndrome coronavirus 2 ; Western blotting ; automation ; monitoring ; nucleocapsid ; observational studies ; serology ; seroprevalence ; wildlife ; France
Language	English
Dates of publication	2022-09
Size	p. e3400-e3407.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2414822-2
ISSN	1865-1682 ; 1865-1674
ISSN (online)	1865-1682
ISSN	1865-1674
DOI	10.1111/tbed.14663
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Relationship between nasopharyngeal microbiota and patient's susceptibility to viral infection.

Dubourg, Grégory / Edouard, Sophie / Raoult, Didier

Expert review of anti-infective therapy

2019 Volume 17, Issue 6, Page(s) 437–447

Abstract: ... ...

Abstract	Introduction
MeSH term(s)	Adult ; Bronchiolitis/epidemiology ; Bronchiolitis/virology ; Child ; Disease Susceptibility ; Humans ; Microbiota ; Nasopharynx/microbiology ; Respiratory Tract Infections/epidemiology ; Respiratory Tract Infections/virology ; Virus Diseases/epidemiology ; Virus Diseases/virology
Language	English
Publishing date	2019-05-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2181279-2
ISSN	1744-8336 ; 1478-7210
ISSN (online)	1744-8336
ISSN	1478-7210
DOI	10.1080/14787210.2019.1621168
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Zs.A 6106: Show issues

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Article ; Online: Sero-epidemiology investigation of Coxiella burnetii in domestic ruminants throughout most Greek regions.

Vourvidis, Dimitrios / Kyrma, Anna / Linou, Maria / Edouard, Sophie / Angelakis, Emmanouil

Veterinary medicine and science

2020 Volume 7, Issue 1, Page(s) 99–104

Abstract: Q fever is not considered as a public health problem in Greece where most regions are considered as Coxiella burnetii free possibly because of the low interest for this agent. Our objective was to conduct a large-scale study to investigate the sero- ... ...

Abstract	Q fever is not considered as a public health problem in Greece where most regions are considered as Coxiella burnetii free possibly because of the low interest for this agent. Our objective was to conduct a large-scale study to investigate the sero-epidemiology of C. burnetii in domestic ruminants throughout the most of Greek regions. We tested serum samples obtained from goats, sheep and bovines from different regions of Greece. All sera were tested for C. burnetii IgG antibodies by a commercial ELISA according to the manufacturer's recommendations. We tested 1,173 goats and sheep obtained from 177 different herds and totally 194 (17%) animals from 78 (44%) herds were positive for C. burnetii. Positive animals were present in seven (88%) different regions and seropositivity varied widely among these regions. The highest percentage was observed in Peloponnese (44%), where all the tested herds presented animals with C. burnetii antibodies. Ιn all Aegean Islands except the island of Limnos we detected goats and sheep positive for C. burnetii with seroposivity varying between 2% in Kos to 37% in Rhodes. Finally, in 22 (85%) Greek prefectures we found C. burnetii IgG-positive animals whereas in 14 (54%) prefectures more than 50% of tested herds had seropositive animals. We also tested 28 cows from five different herds in Macedonia and Aegean Islands and six (21%) of them, obtained from two (40%) herds were positive. Considering the importance of C. burnetii for public health, our data reflect the lack of awareness by veterinarians, physicians and competent authorities as we provide evidence of C. burnetii seropositivity in productive animals throughout the most of Greek territories. Due to the increased risk of inhalation of the bacterium by people who entered the affected farms we raise the question of Q fever emergence in Greece.
MeSH term(s)	Animals ; Cattle ; Cattle Diseases/epidemiology ; Cattle Diseases/microbiology ; Coxiella burnetii/isolation & purification ; Enzyme-Linked Immunosorbent Assay/veterinary ; Goat Diseases/epidemiology ; Goat Diseases/microbiology ; Goats ; Greece/epidemiology ; Prevalence ; Q Fever/epidemiology ; Q Fever/microbiology ; Q Fever/veterinary ; Seroepidemiologic Studies ; Sheep ; Sheep Diseases/epidemiology ; Sheep Diseases/microbiology ; Sheep, Domestic
Language	English
Publishing date	2020-08-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2819409-3
ISSN	2053-1095 ; 2053-1095
ISSN (online)	2053-1095
ISSN	2053-1095
DOI	10.1002/vms3.337
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Article ; Online: Lyophilization to improve the sensitivity of qPCR for bacterial DNA detection in serum: the Q fever paradigm.

Edouard, Sophie / Raoult, Didier

Journal of medical microbiology

2016 Volume 65, Issue 6, Page(s) 462–467

Abstract: Quantitative real-time PCR (qPCR) on serum provides significant added value to the diagnosis of Q fever, mainly at the acute stage of the disease in seronegative patients and in patients with endocarditis. We evaluated the benefits of Coxiella burnetii ... ...

Abstract	Quantitative real-time PCR (qPCR) on serum provides significant added value to the diagnosis of Q fever, mainly at the acute stage of the disease in seronegative patients and in patients with endocarditis. We evaluated the benefits of Coxiella burnetii DNA concentration in serum by lyophilization to improve qPCR sensitivity. The detection limit of qPCR was determined by comparing six 10-fold dilutions of serum (calibrated with 104 bacteria ml-1) with and without lyophilization. We also tested, after lyophilization, 73 sera from patients with acute Q fever and 10 sera from patients with endocarditis for which specific qPCR for C. burnetii performed under our usual conditions remained negative. Lyophilization of DNA was found to improve sensitivity of the qPCR; the limit of detection of C. burnetii DNA by qPCR was 100-fold lower in lyophilized sera (1 bacterium ml-1) than in non-lyophilized sera (102 bacteria ml-1). Among the 73 sera from patients with acute Q fever, 26 (36 %) were positive after lyophilization, demonstrating a sensitivity gain of 44 % for early negative sera and 30 % for positive sera compared to our usual qPCR conditions. Sensitivity was also higher in sera from patients with endocarditis for which 8/10 (80 %) were positive after lyophilization. Our results serve as a proof of concept that lyophilization increases the sensitivity of qPCR in serum by concentrating bacterial DNA. This technique may be applied for the earlier diagnosis of other fastidious bacteria or viruses and extended to other clinical samples.
MeSH term(s)	Coxiella burnetii/genetics ; Coxiella burnetii/isolation & purification ; DNA, Bacterial/blood ; DNA, Bacterial/isolation & purification ; Freeze Drying ; Humans ; Polymerase Chain Reaction/methods ; Q Fever/blood ; Q Fever/microbiology ; Sensitivity and Specificity
Chemical Substances	DNA, Bacterial
Language	English
Publishing date	2016-03-23
Publishing country	England
Document type	Journal Article
ZDB-ID	218356-0
ISSN	1473-5644 ; 0022-2615
ISSN (online)	1473-5644
ISSN	0022-2615
DOI	10.1099/jmm.0.000253
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Article ; Online: Evidence of antibodies against SARS-CoV-2 in wild mustelids from Brittany (France).

Davoust, Bernard / Guérin, Patrick / Orain, Nicolas / Fligny, Camille / Flirden, Fabien / Fenollar, Florence / Mediannikov, Oleg / Edouard, Sophie

Transboundary and emerging diseases

2022 Volume 69, Issue 5, Page(s) e3400–e3407

Abstract: In the French region of Brittany, mainly in the department of the Côtes d'Armor, during the first half of 2021, seropositivity for SARS-CoV-2 was detected in five wild mustelids out of 33 animals tested (15.6%). Anti-SARS-CoV-2 IgG was detected against ... ...

Abstract	In the French region of Brittany, mainly in the department of the Côtes d'Armor, during the first half of 2021, seropositivity for SARS-CoV-2 was detected in five wild mustelids out of 33 animals tested (15.6%). Anti-SARS-CoV-2 IgG was detected against at least four out of five recombinant viral proteins (S1 receptor binding domain, nucleocapsid, S1 subunit, S2 subunit and spike) in three pine martens (Martes martes) and in two badgers (Meles meles) using the automated western blot technique. An ELISA test also identified seropositive cases, although these did not align with western blot results. Although the 171 qPCRs carried out on samples from the 33 mustelids were all negative, these preliminary results from this observational study nevertheless bear witness to infections of unknown origin. The epidemiological surveillance of Covid-19 in wildlife must continue, in particular with effective serology tools.
MeSH term(s)	Animals ; Antibodies, Viral ; COVID-19/epidemiology ; COVID-19/veterinary ; Immunoglobulin G ; Mustelidae ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Viral Proteins
Chemical Substances	Antibodies, Viral ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; Viral Proteins ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-07-27
Publishing country	Germany
Document type	Journal Article ; Observational Study, Veterinary
ZDB-ID	2414822-2
ISSN	1865-1682 ; 1865-1674
ISSN (online)	1865-1682
ISSN	1865-1674
DOI	10.1111/tbed.14663
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Article: Contagion Management at the Méditerranée Infection University Hospital Institute.

Fournier, Pierre-Edouard / Edouard, Sophie / Wurtz, Nathalie / Raclot, Justine / Bechet, Marion / Zandotti, Christine / Filosa, Véronique / Raoult, Didier / Fenollar, Florence

Journal of clinical medicine

2021 Volume 10, Issue 12

Abstract: The Méditerranée Infection University Hospital Institute (IHU) is located in a recent building, which includes experts on a wide range of infectious disease. The IHU strategy is to develop innovative tools, including epidemiological monitoring, point-of- ... ...

Abstract	The Méditerranée Infection University Hospital Institute (IHU) is located in a recent building, which includes experts on a wide range of infectious disease. The IHU strategy is to develop innovative tools, including epidemiological monitoring, point-of-care laboratories, and the ability to mass screen the population. In this study, we review the strategy and guidelines proposed by the IHU and its application to the COVID-19 pandemic and summarise the various challenges it raises. Early diagnosis enables contagious patients to be isolated and treatment to be initiated at an early stage to reduce the microbial load and contagiousness. In the context of the COVID-19 pandemic, we had to deal with a shortage of personal protective equipment and reagents and a massive influx of patients. Between 27 January 2020 and 5 January 2021, 434,925 nasopharyngeal samples were tested for the presence of SARS-CoV-2. Of them, 12,055 patients with COVID-19 were followed up in our out-patient clinic, and 1888 patients were hospitalised in the Institute. By constantly adapting our strategy to the ongoing situation, the IHU has succeeded in expanding and upgrading its equipment and improving circuits and flows to better manage infected patients.
Language	English
Publishing date	2021-06-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm10122627
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Article ; Online: Automated Western immunoblotting detection of anti-SARS-CoV-2 serum antibodies.

Edouard, Sophie / Jaafar, Rita / Orain, Nicolas / Parola, Philippe / Colson, Philippe / La Scola, Bernard / Fournier, Pierre-Edouard / Raoult, Didier / Drancourt, Michel

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2021 Volume 40, Issue 6, Page(s) 1309–1317

Abstract: ELISA and chemiluminescence serological assays for COVID-19 are currently incorporating only one or two SARS-CoV-2 antigens. We developed an automated Western immunoblotting as a complementary serologic assay for COVID-19. The ... ...

Abstract	ELISA and chemiluminescence serological assays for COVID-19 are currently incorporating only one or two SARS-CoV-2 antigens. We developed an automated Western immunoblotting as a complementary serologic assay for COVID-19. The Jess
MeSH term(s)	Antibodies, Viral/blood ; Antibodies, Viral/isolation & purification ; Automation, Laboratory ; Blotting, Western ; COVID-19/diagnosis ; Coronavirus Nucleocapsid Proteins/immunology ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay ; Humans ; Phosphoproteins/immunology ; SARS-CoV-2/immunology ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/immunology
Chemical Substances	Antibodies, Viral ; Coronavirus Nucleocapsid Proteins ; Phosphoproteins ; Spike Glycoprotein, Coronavirus ; nucleocapsid phosphoprotein, SARS-CoV-2 ; spike protein, SARS-CoV-2
Language	English
Publishing date	2021-03-03
Publishing country	Germany
Document type	Comparative Study ; Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-021-04203-8
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