Article ; Online: Mutational Analysis of
2022 Volume 204, Issue 7, Page(s) e0010922
Abstract: The symbiont Vibrio fischeri uses motility to colonize its host. In numerous bacterial species, motility is negatively controlled by cyclic-di-GMP (c-di-GMP), which is produced by diguanylate cyclases (DGCs) with GGDEF domains and degraded by ... ...
| Abstract | The symbiont Vibrio fischeri uses motility to colonize its host. In numerous bacterial species, motility is negatively controlled by cyclic-di-GMP (c-di-GMP), which is produced by diguanylate cyclases (DGCs) with GGDEF domains and degraded by phosphodiesterases with either EAL or HD-GYP domains. To begin to decode the functions of the 50 Vibrio fischeri genes with GGDEF, EAL, and/or HD-GYP domains, we deleted each gene and assessed each mutant's migration through tryptone broth salt (TBS) soft agar medium containing or lacking magnesium (Mg) and calcium (Ca), which are known to influence V. fischeri motility. We identified 6, 13, and 16 mutants with altered migration in TBS-Mg, TBS, and TBS-Ca soft agar, respectively, a result that underscores the importance of medium conditions in assessing gene function. A biosensor-based assay revealed that Mg and Ca affected c-di-GMP levels negatively and positively, respectively; the severe decrease in c-di-GMP caused by Mg addition correlates with its strong positive impact on bacterial migration. A mutant defective for |
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| MeSH term(s) | Agar ; Aliivibrio fischeri/genetics ; Aliivibrio fischeri/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms ; Calcium/metabolism ; Cyclic GMP/analogs & derivatives ; Cyclic GMP/metabolism ; Escherichia coli Proteins/metabolism ; Gene Expression Regulation, Bacterial ; Magnesium/metabolism ; Vibrio cholerae/metabolism |
| Chemical Substances | Bacterial Proteins ; Escherichia coli Proteins ; bis(3',5')-cyclic diguanylic acid (61093-23-0) ; Agar (9002-18-0) ; Cyclic GMP (H2D2X058MU) ; Magnesium (I38ZP9992A) ; Calcium (SY7Q814VUP) |
| Language | English |
| Publishing date | 2022-06-27 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural |
| ZDB-ID | 2968-3 |
| ISSN | 1098-5530 ; 0021-9193 |
| ISSN (online) | 1098-5530 |
| ISSN | 0021-9193 |
| DOI | 10.1128/jb.00109-22 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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