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  1. Article ; Online: Optimum Processing Conditions for Bakasang Using the Response Surface Methodology with Central Composite Design (CCD).

    Fatimah, Feti / Gugule, Sanusi / Alfreds Rorong, Johnly / Ekawati Tallei, Trina

    Pakistan journal of biological sciences : PJBS

    2022  Volume 24, Issue 12, Page(s) 1269–1277

    Abstract: ... Background and Objective: ... Bakasang is a typical food of North Sulawesi and its surroundings, made from fermented fish viscera. This food is made by fermentation so that it is rich in amino acids and polypeptides. This study aimed to determine the ... ...

    Abstract Background and Objective: Bakasang is a typical food of North Sulawesi and its surroundings, made from fermented fish viscera. This food is made by fermentation so that it is rich in amino acids and polypeptides. This study aimed to determine the optimum processing conditions for Bakasang with Peroxide Value (PV) parameters based on Response Surface Methodology (RSM) with Central Composite Design (CCD). Materials and Methods: Viscera from fresh Skipjack tuna consisting of intestines, liver, heart and eggs were mixed with salt and left in a fermenter for varying times and temperatures to obtain Bakasang. The fermentation temperature, salt content, and fermentation time, determined based on the Central Composite Design (CCD), were variables in this study. Thus, 20 experiments consisted of eight quadratic points, six centre points and six axial points. Results: The results showed that the correlation test between temperature, salinity and fermentation time variables on PV were 0.521, 0.305 and 0.591. The regression test resulted in an R<sup>2</sup> value of 0.988. The model equation obtained was y = 4.35194 - 0.11363x<sub>1 </sub>- 0.07459x<sub>2 </sub>- 0.25300x<sub>3 </sub>+ 0.00157x<sub>12 </sub>+ 0.00310x<sub>22</sub> + 0.01571x<sub>32</sub> - 0.00064x<sub>1</sub>x<sub>2 </sub>+ 0.00172x<sub>1</sub>x<sub>3</sub> + 0.00340x<sub>2</sub>x<sub>3</sub>. The contour plot graph obtained from the experiment using CCD showed that the optimum processing conditions for Bakasang were at a temperature of 32.9324°C, salinity 10.2631% and fermentation time of 4.7793 days, which will produce Bakasang with an optimum PV of 1.51256 meq kg<sup>1</sup> sample. Conclusion: According to the findings of this study, the optimum temperature, salinity and fermentation time for producing Bakasang with a PV of 1.51256 meq kg<sup>1</sup> sample were 32.932°C, 10.263% and 4.779 days, respectively.
    MeSH term(s) Animals ; Crop Production/methods ; Crop Production/standards ; Fermented Foods/standards ; Fermented Foods/supply & distribution ; Fishes/metabolism ; Fishes/microbiology ; Indonesia
    Language English
    Publishing date 2022-01-05
    Publishing country Pakistan
    Document type Journal Article
    ISSN 1812-5735
    ISSN (online) 1812-5735
    DOI 10.3923/pjbs.2021.1269.1277
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Indigenous Lactic Acid Bacteria Isolated from Spontaneously Fermented Goat Milk as Potential Probiotics.

    Yelnetty, Afriza / Purwadi / Ekawati Tallei, Trina

    Pakistan journal of biological sciences : PJBS

    2020  Volume 23, Issue 7, Page(s) 883–890

    Abstract: Background and objective: Lactic Acid Bacteria (LAB) isolated from goat milk have been known to have various medicinal properties, therefore they are considered as a source of potential probiotics. This research was aimed at evaluating and identifying ... ...

    Abstract Background and objective: Lactic Acid Bacteria (LAB) isolated from goat milk have been known to have various medicinal properties, therefore they are considered as a source of potential probiotics. This research was aimed at evaluating and identifying the LAB isolated from spontaneously fermented goat milk as potential probiotics.
    Materials and methods: The fresh goat milk fermented for 4 days was serially diluted, plated on de Man Rogosa and Sharpe (MRS) agar supplemented with 1% CaCO3 as selective medium, then purified accordingly. The isolated LAB were screened for their potential to inhibit enteric pathogen bacteria using well diffusion method. Their capabilities to withstand the bile salt and acid environment were also evaluated. The production of organic acids was also assessed. The potential probiotics were identified molecularly using 16S rRNA.
    Results: The study confirmed that LAB isolated from spontaneous fermentation of goat milk was Lactobacillus plantarum based on 16S rRNA gene marker. This bacterium showed antimicrobial activity against indicator bacteria, ability to live after exposure in bile salt solution and resistance to low acidic environment. The organic acids produced by this bacterium were lactic, acetic, propionic and butyric acids.
    Conclusion: This study concluded that Lactobacillus plantarum YN.1.3 can be further investigated as potential probiotic as it showed antimicrobial activity, withstood the acidic environment and bile salt solution, as well as produced organic acids.
    Language English
    Publishing date 2020-07-23
    Publishing country Pakistan
    Document type Journal Article
    ISSN 1812-5735
    ISSN (online) 1812-5735
    DOI 10.3923/pjbs.2020.883.890
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Potential of Organic Mercury-resistant Bacteria Isolated from Mercury Contaminated Sites for Organic Mercury Remediation.

    Fatimawali / Kepel, Billy / Ekawati Tallei, Trina

    Pakistan journal of biological sciences : PJBS

    2019  Volume 22, Issue 1, Page(s) 45–50

    Abstract: Background and objective: Efforts at organic mercury detoxification can be carried out using resistant bacteria that can live in an environment contaminated with the compound. This study aimed at isolating and identifying resistant bacteria from mercury- ...

    Abstract Background and objective: Efforts at organic mercury detoxification can be carried out using resistant bacteria that can live in an environment contaminated with the compound. This study aimed at isolating and identifying resistant bacteria from mercury-contaminated environments and analysing their ability to detoxify organic mercury.
    Materials and methods: Soil samples were obtained from 3 gold processing locations that make use of mercury in Tanoyan Village, Bolaang Mongondow district, North Sulawesi province. The identification was carried out on the mercury-resistant bacteria through morphological and molecular tests. Bacteria which were highly resistant to mercury were examined for their ability to detoxify phenyl mercury (organic mercury).
    Results: The study showed that 8 mercury-resistant bacterial colonies could be isolated from the three soil samples. The bacteria were able to grow in LB broth containing 10 mg L-1 of phenyl mercury. Four isolates (AA, BB, CC and DD) were even able to grow in 40 mg L-1 of phenyl mercury. According to the identification tests, those bacteria were Pseudomonas sp. (AA, DD), Pseudomonas aeruginosa (BB) and Proteus mirabilis (CC). Testing of organic mercury against isolates of bacteria which are highly resistant to it in order to determine their detoxification capacity revealed that all four isolates could reduce levels of the compound in media, based on the results, starting from the highest was Pseudomonas sp. 74.99%, then Pseudomonas aeruginosa 60.23% and Proteus mirabilis 47.59% after 24 h of incubation.
    Conclusion: The study suggested that there are four bacteria that have potentials to remediate organic mercury contamination sites.
    MeSH term(s) Bacteria/isolation & purification ; Biodegradation, Environmental ; Indonesia ; Mercury/adverse effects ; Proteus mirabilis/isolation & purification ; Pseudomonas/isolation & purification
    Chemical Substances Mercury (FXS1BY2PGL)
    Language English
    Publishing date 2019-02-22
    Publishing country Pakistan
    Document type Journal Article
    ISSN 1028-8880
    ISSN 1028-8880
    DOI 10.3923/pjbs.2019.45.50
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Phytochemical and Antioxidant Activities of

    Jeane Damongilala, Lena / Wewengkang, Defny Silvia / Losung, Fitje / Ekawati Tallei, Trina

    Pakistan journal of biological sciences : PJBS

    2021  Volume 24, Issue 1, Page(s) 132–138

    Abstract: Background and objective: Research to find functional food from new natural sources has caught the attention of many researchers through the characterization of phytochemicals and biological activities. One potential source of natural ingredients is the ...

    Abstract Background and objective: Research to find functional food from new natural sources has caught the attention of many researchers through the characterization of phytochemicals and biological activities. One potential source of natural ingredients is the red alga Eucheuma spinosum, which has been used as a daily source of natural food. The purpose of this study was to obtain a prospective new source of natural antioxidants from various extracts of tropical red alga (E. spinosum) through several tests, which were used as determinants of whether the alga can be used as a functional food source.
    Materials and methods: Algal sample was extracted with organic solvents (methanol, n-hexane, ethyl-acetate and water) and purified by a combination of normal and reverse phase chromatography methods.
    Results: The algal extracts had antioxidant compounds based on free radical scavenging activities using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and superoxide dismutase (SOD). The ethyl acetate extract of E. spinosum scavenged DPPH and SOD free radicals, so that this extract was indicated contained powerful antioxidants. The result of the isolation of the antioxidant compound showed the presence of pure compound 3-(3-methoxyphenyl) propanal.
    Conclusion: This study concluded that red algae E. spinosum contained natural antioxidants which have the potential to be developed as a functional food and disease prevention and treatment. In addition, the components of these antioxidant compounds from the algae have the potential to be used as natural sources of new functional ingredients.
    MeSH term(s) Antioxidants/isolation & purification ; Antioxidants/pharmacology ; Functional Food ; Indonesia ; Molecular Structure ; Nutritive Value ; Phytochemicals/isolation & purification ; Phytochemicals/pharmacology ; Rhodophyta/chemistry ; Structure-Activity Relationship
    Chemical Substances Antioxidants ; Phytochemicals
    Language English
    Publishing date 2021-03-08
    Publishing country Pakistan
    Document type Journal Article
    ISSN 1812-5735
    ISSN (online) 1812-5735
    DOI 10.3923/pjbs.2021.132.138
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: The depsidones from marine sponge-derived fungus

    Handayani, Dian / Aminah, Ibtisamatul / Pontana Putra, Purnawan / Eka Putra, Andani / Arbain, Dayar / Satriawan, Herland / Efdi, Mai / Celik, Ismail / Ekawati Tallei, Trina

    Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

    2023  Volume 31, Issue 9, Page(s) 101744

    Abstract: Methicillin- ... ...

    Abstract Methicillin-resistant
    Language English
    Publishing date 2023-08-09
    Publishing country Saudi Arabia
    Document type Journal Article
    ZDB-ID 1378024-4
    ISSN 1319-0164
    ISSN 1319-0164
    DOI 10.1016/j.jsps.2023.101744
    Database MEDical Literature Analysis and Retrieval System OnLINE

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