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  1. Article: SARS-CoV-2 Rapid Antigen Testing of Symptomatic and Asymptomatic Individuals on the University of Arizona Campus.

    Harris, David T / Badowski, Michael / Jernigan, Brandon / Sprissler, Ryan / Edwards, Taylor / Cohen, Randall / Paul, Stephen / Merchant, Nirav / Weinkauf, Craig C / Bime, Christian / Erickson, Heidi E / Bixby, Billie / Parthasarathy, Sairam / Chaudhary, Sachin / Natt, Bhupinder / Cristan, Elaine / El Aini, Tammer / Rischard, Franz / Campion, Janet /
    Chopra, Madhav / Insel, Michael / Sam, Afshin / Knepler, James L / Knox, Kenneth / Mosier, Jarrod / Spier, Catherine / Dake, Michael D

    Biomedicines

    2021  Volume 9, Issue 5

    Abstract: SARS-CoV-2, the cause of COVID19, has caused a pandemic that has infected more than 80 M and killed more than 1.6 M persons worldwide. In the US as of December 2020, it has infected more than 32 M people while causing more than 570,000 deaths. As the ... ...

    Abstract SARS-CoV-2, the cause of COVID19, has caused a pandemic that has infected more than 80 M and killed more than 1.6 M persons worldwide. In the US as of December 2020, it has infected more than 32 M people while causing more than 570,000 deaths. As the pandemic persists, there has been a public demand to reopen schools and university campuses. To consider these demands, it is necessary to rapidly identify those individuals infected with the virus and isolate them so that disease transmission can be stopped. In the present study, we examined the sensitivity of the Quidel Rapid Antigen test for use in screening both symptomatic and asymptomatic individuals at the University of Arizona from June to August 2020. A total of 885 symptomatic and 1551 asymptomatic subjects were assessed by antigen testing and real-time PCR testing. The sensitivity of the test for both symptomatic and asymptomatic persons was between 82 and 90%, with some caveats.
    Language English
    Publishing date 2021-05-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines9050539
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity.

    Ripperger, Tyler J / Uhrlaub, Jennifer L / Watanabe, Makiko / Wong, Rachel / Castaneda, Yvonne / Pizzato, Hannah A / Thompson, Mallory R / Bradshaw, Christine / Weinkauf, Craig C / Bime, Christian / Erickson, Heidi L / Knox, Kenneth / Bixby, Billie / Parthasarathy, Sairam / Chaudhary, Sachin / Natt, Bhupinder / Cristan, Elaine / El Aini, Tammer / Rischard, Franz /
    Campion, Janet / Chopra, Madhav / Insel, Michael / Sam, Afshin / Knepler, James L / Capaldi, Andrew P / Spier, Catherine M / Dake, Michael D / Edwards, Taylor / Kaplan, Matthew E / Scott, Serena Jain / Hypes, Cameron / Mosier, Jarrod / Harris, David T / LaFleur, Bonnie J / Sprissler, Ryan / Nikolich-Žugich, Janko / Bhattacharya, Deepta

    Immunity

    2020  Volume 53, Issue 5, Page(s) 925–933.e4

    Abstract: We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies ... ...

    Abstract We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies against the nucleocapsid (N) and the receptor binding domain (RBD) of the spike protein. Age and sex played lesser roles. All cases, including asymptomatic individuals, seroconverted by 2 weeks after PCR confirmation. Spike RBD and S2 and neutralizing antibodies remained detectable through 5-7 months after onset, whereas α-N titers diminished. Testing 5,882 members of the local community revealed only 1 sample with seroreactivity to both RBD and S2 that lacked neutralizing antibodies. This fidelity could not be achieved with either RBD or S2 alone. Thus, inclusion of multiple independent assays improved the accuracy of antibody tests in low-seroprevalence communities and revealed differences in antibody kinetics depending on the antigen. We conclude that neutralizing antibodies are stably produced for at least 5-7 months after SARS-CoV-2 infection.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Arizona/epidemiology ; Betacoronavirus/immunology ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/blood ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Coronavirus Infections/immunology ; Coronavirus Nucleocapsid Proteins ; Female ; Humans ; Immunity, Humoral ; Male ; Middle Aged ; Nucleocapsid Proteins/immunology ; Pandemics ; Phosphoproteins ; Pneumonia, Viral/blood ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/immunology ; Prevalence ; Protein Interaction Domains and Motifs ; SARS-CoV-2 ; Seroepidemiologic Studies ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/immunology ; Young Adult
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Coronavirus Nucleocapsid Proteins ; Nucleocapsid Proteins ; Phosphoproteins ; Spike Glycoprotein, Coronavirus ; nucleocapsid phosphoprotein, SARS-CoV-2 ; spike protein, SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-10-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1217235-2
    ISSN 1097-4180 ; 1074-7613
    ISSN (online) 1097-4180
    ISSN 1074-7613
    DOI 10.1016/j.immuni.2020.10.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Detection, prevalence, and duration of humoral responses to SARS-CoV-2 under conditions of limited population exposure.

    Ripperger, Tyler J / Uhrlaub, Jennifer L / Watanabe, Makiko / Wong, Rachel / Castaneda, Yvonne / Pizzato, Hannah A / Thompson, Mallory R / Bradshaw, Christine / Weinkauf, Craig C / Bime, Christian / Erickson, Heidi L / Knox, Kenneth / Bixby, Billie / Parthasarathy, Sairam / Chaudhary, Sachin / Natt, Bhupinder / Cristan, Elaine / El Aini, Tammer / Rischard, Franz /
    Campion, Janet / Chopra, Madhav / Insel, Michael / Sam, Afshin / Knepler, James L / Capaldi, Andrew P / Spier, Catherine M / Dake, Michael D / Edwards, Taylor / Kaplan, Matthew E / Scott, Serena Jain / Hypes, Cameron / Mosier, Jarrod / Harris, David T / Lafleur, Bonnie J / Sprissler, Ryan / Nikolich-Zugich, Janko / Bhattacharya, Deepta

    medRxiv

    Abstract: We conducted an extensive serological study to quantify population-level exposure and define correlates of immunity against SARS-CoV-2. We found that relative to mild COVID-19 cases, individuals with severe disease exhibited elevated authentic virus- ... ...

    Abstract We conducted an extensive serological study to quantify population-level exposure and define correlates of immunity against SARS-CoV-2. We found that relative to mild COVID-19 cases, individuals with severe disease exhibited elevated authentic virus-neutralizing titers and antibody levels against nucleocapsid (N) and the receptor binding domain (RBD) and the S2 region of spike protein. Unlike disease severity, age and sex played lesser roles in serological responses. All cases, including asymptomatic individuals, seroconverted by 2 weeks post-PCR confirmation. RBD- and S2-specific and neutralizing antibody titers remained elevated and stable for at least 2-3 months post-onset, whereas those against N were more variable with rapid declines in many samples. Testing of 5882 self-recruited members of the local community demonstrated that 1.24% of individuals showed antibody reactivity to RBD. However, 18% (13/73) of these putative seropositive samples failed to neutralize authentic SARS-CoV-2 virus. Each of the neutralizing, but only 1 of the non-neutralizing samples, also displayed potent reactivity to S2. Thus, inclusion of multiple independent assays markedly improved the accuracy of antibody tests in low seroprevalence communities and revealed differences in antibody kinetics depending on the viral antigen. In contrast to other reports, we conclude that immunity is durable for at least several months after SARS-CoV-2 infection.
    Keywords covid19
    Language English
    Publishing date 2020-08-16
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2020.08.14.20174490
    Database COVID19

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  4. Article: Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity

    Ripperger, Tyler J / Uhrlaub, Jennifer L / Watanabe, Makiko / Wong, Rachel / Castaneda, Yvonne / Pizzato, Hannah A / Thompson, Mallory R / Bradshaw, Christine / Weinkauf, Craig C / Bime, Christian / Erickson, Heidi L / Knox, Kenneth / Bixby, Billie / Parthasarathy, Sairam / Chaudhary, Sachin / Natt, Bhupinder / Cristan, Elaine / El Aini, Tammer / Rischard, Franz /
    Campion, Janet / Chopra, Madhav / Insel, Michael / Sam, Afshin / Knepler, James L / Capaldi, Andrew P / Spier, Catherine M / Dake, Michael D / Edwards, Taylor / Kaplan, Matthew E / Scott, Serena Jain / Hypes, Cameron / Mosier, Jarrod / Harris, David T / LaFleur, Bonnie J / Sprissler, Ryan / Nikolich-Zugich, Janko / Bhattacharya, Deepta

    Immunity

    Abstract: We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies ... ...

    Abstract We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies against the nucleocapsid (N) and the receptor binding domain (RBD) of the spike protein. Age and sex played lesser roles. All cases, including asymptomatic individuals, seroconverted by 2 weeks after PCR confirmation. Spike RBD and S2 and neutralizing antibodies remained detectable through 5-7 months after onset, whereas α-N titers diminished. Testing 5,882 members of the local community revealed only 1 sample with seroreactivity to both RBD and S2 that lacked neutralizing antibodies. This fidelity could not be achieved with either RBD or S2 alone. Thus, inclusion of multiple independent assays improved the accuracy of antibody tests in low-seroprevalence communities and revealed differences in antibody kinetics depending on the antigen. We conclude that neutralizing antibodies are stably produced for at least 5-7 months after SARS-CoV-2 infection.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #856763
    Database COVID19

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  5. Article ; Online: Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity

    Ripperger, Tyler J. / Uhrlaub, Jennifer L. / Watanabe, Makiko / Wong, Rachel / Castaneda, Yvonne / Pizzato, Hannah A. / Thompson, Mallory R. / Bradshaw, Christine / Weinkauf, Craig C. / Bime, Christian / Erickson, Heidi L. / Knox, Kenneth / Bixby, Billie / Parthasarathy, Sairam / Chaudhary, Sachin / Natt, Bhupinder / Cristan, Elaine / El Aini, Tammer / Rischard, Franz /
    Campion, Janet / Chopra, Madhav / Insel, Michael / Sam, Afshin / Knepler, James L. / Capaldi, Andrew P. / Spier, Catherine M. / Dake, Michael D. / Edwards, Taylor / Kaplan, Matthew E. / Scott, Serena Jain / Hypes, Cameron / Mosier, Jarrod / Harris, David T. / LaFleur, Bonnie J. / Sprissler, Ryan / Nikolich-Žugich, Janko / Bhattacharya, Deepta

    Immunity

    2020  Volume 53, Issue 5, Page(s) 925–933.e4

    Keywords Immunology ; Immunology and Allergy ; Infectious Diseases ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 1217235-2
    ISSN 1097-4180 ; 1074-7613
    ISSN (online) 1097-4180
    ISSN 1074-7613
    DOI 10.1016/j.immuni.2020.10.004
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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