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Article ; Online: Systematic review and meta-analysis for the biotechnological production of THC in Morocco

badrana Fadwa / Mokhtari Abdelrhani / Safini Najete / Soulaymani Abdelmajid / El fahime Elmostafa / El Amri Hamid

E3S Web of Conferences, Vol 319, p

2021 Volume 02012

Abstract: Cannabinoids have promising therapeutic potential. Δ9-tetrahydrocannabinol (THC), the most important psychotropic active ingredient of Cannabis Sativa L, has been the subject of several chemical, pharmacological and biosynthetic studies. In this context, ...

Abstract	Cannabinoids have promising therapeutic potential. Δ9-tetrahydrocannabinol (THC), the most important psychotropic active ingredient of Cannabis Sativa L, has been the subject of several chemical, pharmacological and biosynthetic studies. In this context, a meta-analysis of biotechnological processes applied to the production of recombinant cannabinoid THC worldwide was carried out. The objective was to highlight the potential of these processes on the Moroccan variety of Cannabis Sativa. The PubMed, ScienceDirect and Web of Science search motors were used to search for original scientific work presenting biotechnological tools used in the production of THC. The scientific articles exploited are those published before the end of 2020. Succinct analysis of the experimental work performed showed that expression of the gene encoding Cannabis Sativa L. THCA synthase was performed on prokaryotic and eukaryotic expression systems. Currently, no functional expression could be obtained in E. coli. Whereas, production of recombinant protein (THCA Synthase) associated with significant enzymatic activity was obtained in P. pastoris cultures (F. Saccharomycetaceae). The exploitation of the sequences showed the presence of a large similarity between the THCA Synthase gene of cannabis Sativa of the Moroccan variety and the mRNA precursor of the same gene reported in several studies. This will allow us to use a specific signal sequence of choice for an adopted expression host, in order to produce the recombinant THCA synthase enzyme from the Moroccan Cannabis Sativa L strain on P. pastoris cultures.
Keywords	Environmental sciences ; GE1-350
Subject code	500
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Chapter 27 Emergence and Reemergence of Viral Zoonotic Diseases: Concepts and Factors of Emerging and Reemerging Globalization of Health Threats

El Amri, Hamid / Boukharta, Mohamed / Zakham, Fathiah / Ennaji, Moulay Mustapha

Emerging and Reemerging Viral Pathogens

Abstract: Abstract The unpredictable emergence of new zoonotic diseases with viral etiology is currently a hot issue in the scientific and political circles. Viral emergence and reemergence, as a sanitary event, are only the visible part of the iceberg, while the ... ...

Abstract	Abstract The unpredictable emergence of new zoonotic diseases with viral etiology is currently a hot issue in the scientific and political circles. Viral emergence and reemergence, as a sanitary event, are only the visible part of the iceberg, while the hidden one corresponds to a multitude of complex and interrelated factors, including societal and environmental factors favoring the advent of the state of viral emergence and reemergence. According to the World Health Organization, 60% of the agents recognized as human pathogens come from the animal kingdom, and 75% of the pathogens responsible for emerging and reemerging animal diseases present a potential transgression of interface between interspecies establishing favorable conditions for genetic exchange leading to the emergence of new highly pathogenic variants and strains of which the animal is often the host reservoir. Therefore any public health prophylactic strategy requires a holistic approach to the health problem, taking into account the interaction between the triad elements of the human, animal, and environment. This globalized approach has been realized by the international community through the new recently established concept of “One Health, One World,” making the old bipolar concept of separated human and animal health issues avoided and obsolete. The extreme diversity of emerging and reemerging viral pathogens, the change of human lifestyle, the globalization of travel, business exchanges, and tourism potentiate the risk of emergence of highly pathogenic zoonotic diseases. Promoting intersectorial collaboration will allow to unify the health and safety policies. The cross-cutting ecological and health data at the national and global scales are effective means for sustaining good health in human, animal, and ecosystem (in particular the viral ecology).
Keywords	covid19
Publisher	Elsevier; PMC
Document type	Article ; Online
DOI	10.1016/b978-0-12-819400-3.00027-2
Database	COVID19

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Article ; Online: Emergence and Reemergence of Viral Zoonotic Diseases: Concepts and Factors of Emerging and Reemerging Globalization of Health Threats

El Amri, Hamid / Boukharta, Mohamed / Zakham, Fathiah / Ennaji, Moulay Mustapha

Emerging and Reemerging Viral Pathogens

Abstract: The unpredictable emergence of new zoonotic diseases with viral etiology is currently a hot issue in the scientific and political circles. Viral emergence and reemergence, as a sanitary event, are only the visible part of the iceberg, while the hidden ... ...

Abstract	The unpredictable emergence of new zoonotic diseases with viral etiology is currently a hot issue in the scientific and political circles. Viral emergence and reemergence, as a sanitary event, are only the visible part of the iceberg, while the hidden one corresponds to a multitude of complex and interrelated factors, including societal and environmental factors favoring the advent of the state of viral emergence and reemergence. According to the World Health Organization, 60% of the agents recognized as human pathogens come from the animal kingdom, and 75% of the pathogens responsible for emerging and reemerging animal diseases present a potential transgression of interface between interspecies establishing favorable conditions for genetic exchange leading to the emergence of new highly pathogenic variants and strains of which the animal is often the host reservoir. Therefore any public health prophylactic strategy requires a holistic approach to the health problem, taking into account the interaction between the triad elements of the human, animal, and environment. This globalized approach has been realized by the international community through the new recently established concept of “One Health, One World,” making the old bipolar concept of separated human and animal health issues avoided and obsolete. The extreme diversity of emerging and reemerging viral pathogens, the change of human lifestyle, the globalization of travel, business exchanges, and tourism potentiate the risk of emergence of highly pathogenic zoonotic diseases. Promoting intersectorial collaboration will allow to unify the health and safety policies. The cross-cutting ecological and health data at the national and global scales are effective means for sustaining good health in human, animal, and ecosystem (in particular the viral ecology).
Keywords	covid19
Publisher	Elsevier; PMC
Document type	Article ; Online
DOI	10.1016/b978-0-12-819400-3.00027-2
Database	COVID19

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Article ; Online: AKT1 Polymorphism (rs10138227) and Risk of Colorectal Cancer in Moroccan Population: A Case Control Study.

Allam, Loubna / Arrouchi, Housna / Ghrifi, Fatima / El Khazraji, Abdelhak / Kandoussi, Ilham / Bendahou, Mohammed Amine / El Amri, Hamid / El Absi, Mohamed / Ibrahimi, Azeddine

Asian Pacific journal of cancer prevention : APJCP

2020 Volume 21, Issue 11, Page(s) 3165–3170

Abstract: Background: LMTK3 and AKT1 each have a role in carcinogenesis and tumor progression. The analysis of single nucleotide polymorphisms of AKT1 and LMTK3 could lead to more complete and accurate risk estimates for colorectal cancer.: Aim: We evaluated ... ...

Abstract	Background: LMTK3 and AKT1 each have a role in carcinogenesis and tumor progression. The analysis of single nucleotide polymorphisms of AKT1 and LMTK3 could lead to more complete and accurate risk estimates for colorectal cancer. Aim: We evaluated the association between single nucleotide polymorphisms (SNPs) of AKT1 and LMTK3 and the risk of colorectal cancer in a case-control study in Moroccan population. Methods: Genomic DNA from 70 colorectal cancer patients and 50 healthy control subjects was extracted from whole blood. Genotyping was performed by direct sequencing after polymerase chain reactions for the 7 SNPs (AKT1rs1130214G/T, AKT1rs10138227C/T, AKT1rs3730358C/T, AKT1rs1000559097G/A, AKT1rs2494737A/T, LMTK3rs8108419G/A, and LMTK3rs9989661A/G.). Study subjects provided detailed information during the collection. All P values come from bilateral tests. Results: In the logistic regression analysis, a significantly high risk of colorectal cancer was associated with TC/TT genotypes of rs10138227 with adjusted odds ratio [OR] equal to 2.82 and 95% confidence interval [CI] of 1.15 to 6.91. Conclusion: Our results suggest that the SNP AKT1rs10138227 could affect susceptibility to CRC, probably by modulating the transcriptional activity of AKT1. However, larger independent studies are needed to validate our results.
MeSH term(s)	Biomarkers, Tumor/genetics ; Case-Control Studies ; Colorectal Neoplasms/epidemiology ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/pathology ; Female ; Follow-Up Studies ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Middle Aged ; Morocco/epidemiology ; Polymorphism, Single Nucleotide ; Prognosis ; Proto-Oncogene Proteins c-akt/genetics ; Risk Factors
Chemical Substances	Biomarkers, Tumor ; AKT1 protein, human (EC 2.7.11.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2020-11-01
Publishing country	Thailand
Document type	Journal Article
ZDB-ID	2218955-5
ISSN	2476-762X ; 1513-7368
ISSN (online)	2476-762X
ISSN	1513-7368
DOI	10.31557/APJCP.2020.21.11.3165
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Detection of Anaplasma spp. and Ehrlichia spp. anibodies, and Dirofilaria immitis antigens in dogs from seven locations of Morocco.

Elhamiani Khatat, Sarah / Khallaayoune, Khalid / Errafyk, Nabil / Van Gool, Frans / Duchateau, Luc / Daminet, Sylvie / Kachani, Malika / El Amri, Hamid / Azrib, Rahma / Sahibi, Hamid

Veterinary parasitology

2017 Volume 239, Page(s) 86–89

Abstract: In Morocco no data has been published on canine exposure to Anaplasma spp., Borrrelia burgdorferi, and Ehrlichia spp., and only one report is available on the occurrence of Dirofilaria immitis in dogs. Therefore, the aim of this study was to collect ... ...

Abstract	In Morocco no data has been published on canine exposure to Anaplasma spp., Borrrelia burgdorferi, and Ehrlichia spp., and only one report is available on the occurrence of Dirofilaria immitis in dogs. Therefore, the aim of this study was to collect current data on the canine exposure to these vector-borne pathogens (VBPs) in Morocco. A total of 217 urban (n=57), rural (n=110) and military (n=50) dogs from seven Moroccan locations were screened for Anaplasma spp., B. burgdorferi and Ehrlichia spp. antibodies and for D. immitis antigens using a commercial in-clinic ELISA test. Of these dogs, 182 (83.9%) tested positive for at least one pathogen and positivity to two or three pathogens was found in 14.3% and 2.3% of the dogs, respectively. Ehrlichia spp. antibodies (34.6%) were the most frequently detected followed by Anaplasma spp. antibodies (16.6%) and D. immitis antigens (16.1%). None of the dogs was tested seropositive to B. burgdorferi. Statistically significant differences in seropositivity rates were found for Ehrlichia spp. and D. immitis in rural dogs especially those from the north central region (p<0.001) but not for Anaplasma spp. No significant difference was found according to the health status of the dog. This study demonstrates that Moroccan dogs are at high risk of acquiring a vector-borne infection.
MeSH term(s)	Anaplasma/immunology ; Anaplasmosis/epidemiology ; Anaplasmosis/immunology ; Animals ; Antibodies, Bacterial/blood ; Antigens, Helminth/blood ; Dirofilaria immitis/immunology ; Dirofilariasis/epidemiology ; Dirofilariasis/immunology ; Dog Diseases/epidemiology ; Dog Diseases/parasitology ; Dogs ; Ehrlichia/immunology ; Ehrlichiosis/epidemiology ; Ehrlichiosis/immunology ; Ehrlichiosis/veterinary ; Female ; Male
Chemical Substances	Antibodies, Bacterial ; Antigens, Helminth
Language	English
Publishing date	2017-05-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	196831-2
ISSN	1873-2550 ; 0304-4017
ISSN (online)	1873-2550
ISSN	0304-4017
DOI	10.1016/j.vetpar.2017.04.004
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Article ; Online: Anaplasma spp. in dogs and owners in north-western Morocco.

Elhamiani Khatat, Sarah / Daminet, Sylvie / Kachani, Malika / Leutenegger, Christian M / Duchateau, Luc / El Amri, Hamid / Hing, Mony / Azrib, Rahma / Sahibi, Hamid

Parasites & vectors

2017 Volume 10, Issue 1, Page(s) 202

Abstract: Background: Anaplasma phagocytophilum is an emerging tick-borne zoonotic pathogen of increased interest worldwide which has been detected in northern Africa. Anaplasma platys is also present in this region and could possibly have a zoonotic potential. ... ...

Abstract	Background: Anaplasma phagocytophilum is an emerging tick-borne zoonotic pathogen of increased interest worldwide which has been detected in northern Africa. Anaplasma platys is also present in this region and could possibly have a zoonotic potential. However, only one recent article reports on the human esposure to A. phagocytophilum in Morocco and no data are available on canine exposure to both bacteria. Therefore, we conducted a cross-sectional epidemiological study aiming to assess both canine and human exposure to Anaplasma spp. in Morocco. A total of 425 dogs (95 urban, 160 rural and 175 working dogs) and 11 dog owners were sampled from four cities of Morocco. Canine blood samples were screened for Anaplasma spp. antibodies by an enzyme-linked immunosorbent assay (ELISA) and for A. phagocytophilum and A. platys DNA by a real-time polymerase chain reaction (RT-PCR) targeting the msp2 gene. Human sera were tested for specific A. phagocytophilum immunoglobulin G (IgG) using a commercial immunofluorescence assay (IFA) kit. Results: Anaplasma spp. antibodies and A. platys DNA were detected in 21.9 and 7.5% of the dogs, respectively. Anaplasma phagocytophilum DNA was not amplified. Anaplasma platys DNA was significantly more frequently amplified for working dogs. No statistically significant differences in the prevalence of Anaplasma spp. antibodies or A. platys DNA detection were observed between sexes, age classes or in relation to exposure to ticks. A total of 348 Rhipicephalus sanguineus (sensu lato) ticks were removed from 35 urban and working dogs. The majority of dog owners (7/10) were seroreactive to A. phagoyctophilum IgG (one sample was excluded because of hemolysis). Conclusions: This study demonstrates the occurrence of Anaplasma spp. exposure and A. platys infection in dogs, and A. phagocytophilum exposure in humans in Morocco.
MeSH term(s)	Adult ; Aged ; Anaplasma/classification ; Anaplasma/isolation & purification ; Anaplasmosis/epidemiology ; Anaplasmosis/parasitology ; Animals ; Dog Diseases/epidemiology ; Dog Diseases/microbiology ; Dogs ; Female ; Humans ; Ixodidae/classification ; Male ; Middle Aged ; Morocco/epidemiology ; Ownership ; Young Adult ; Zoonoses
Language	English
Publishing date	2017-04-24
Publishing country	England
Document type	Journal Article
ZDB-ID	2409480-8
ISSN	1756-3305 ; 1756-3305
ISSN (online)	1756-3305
ISSN	1756-3305
DOI	10.1186/s13071-017-2148-y
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Article ; Online: Human Exposure to Anaplasma phagocytophilum in Two Cities of Northwestern Morocco.

Elhamiani Khatat, Sarah / Sahibi, Hamid / Hing, Mony / Alaoui Moustain, Ismail / El Amri, Hamid / Benajiba, Mohammed / Kachani, Malika / Duchateau, Luc / Daminet, Sylvie

PloS one

2016 Volume 11, Issue 8, Page(s) e0160880

Abstract: Anaplasma phagocytophilum is an emerging tick-borne zoonosis with extensive increased interest. Epidemiological data are available in several regions of the USA, Europe and Asia in contrast to other parts of the world such as North Africa. Blood samples ... ...

Abstract	Anaplasma phagocytophilum is an emerging tick-borne zoonosis with extensive increased interest. Epidemiological data are available in several regions of the USA, Europe and Asia in contrast to other parts of the world such as North Africa. Blood samples of 261 healthy individuals divided in two groups i.e., dog handlers and blood donors were analysed. Indirect immunofluorescent assay using a commercial kit was performed to detect specific A. phagocytophilum IgG. Two dilutions were used to assess the prevalence of seroreactive samples. Demographic variables were assessed as potential risk factors using exact logistic regression. Seropositivity rates reached 37% and 27% in dog handlers and 36% and 22% in blood donors. No statistically significant differences were found in the prevalence rates between the two groups. Analysis of risk factors such as gender, age groups, outdoor activities, self-reported previous exposure to ticks, or contact with domestic animals (dogs, cats, ruminants and horses) did not shown any significant difference. A. phagocytophilum exposure was common in both high-risk population and blood donors in Morocco.
MeSH term(s)	Adolescent ; Adult ; Anaplasma phagocytophilum/immunology ; Anaplasma phagocytophilum/pathogenicity ; Animals ; Animals, Domestic ; Antibodies, Bacterial/blood ; Blood Donors ; Cities ; Dogs ; Ehrlichiosis/epidemiology ; Ehrlichiosis/microbiology ; Ehrlichiosis/transmission ; Female ; Humans ; Immunoglobulin G/blood ; Male ; Middle Aged ; Morocco/epidemiology ; Risk Factors ; Seroepidemiologic Studies ; Young Adult ; Zoonoses/epidemiology ; Zoonoses/microbiology ; Zoonoses/transmission
Chemical Substances	Antibodies, Bacterial ; Immunoglobulin G
Language	English
Publishing date	2016
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0160880
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Results of a collaborative study on DNA identification of aged bone samples.

Vanek, Daniel / Budowle, Bruce / Dubska-Votrubova, Jitka / Ambers, Angie / Frolik, Jan / Pospisek, Martin / Al Afeefi, Ahmed Anwar / Al Hosani, Khalid Ismaeil / Allen, Marie / Al Naimi, Khudooma Saeed / Al Salafi, Dina / Al Tayyari, Wafa Ali Rashid / Arguetaa, Wendy / Bottinelli, Michel / Bus, Magdalena M / Cemper-Kiesslich, Jan / Cepil, Olivier / De Cock, Greet / Desmyter, Stijn /

Croatian medical journal

2017 Volume 58, Issue 3, Page(s) 203–213

Abstract: Aim: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of ... ...

Abstract	Aim: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. Methods: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. Results: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. Conclusion: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
MeSH term(s)	Bone and Bones/chemistry ; Czech Republic ; DNA/analysis ; DNA Fingerprinting/standards ; Forensic Genetics ; Humans
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2017-06-14
Publishing country	Croatia
Document type	Journal Article ; Multicenter Study
ZDB-ID	1157623-6
ISSN	1332-8166 ; 0353-9504
ISSN (online)	1332-8166
ISSN	0353-9504
DOI	10.3325/cmj.2017.58.203
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Article: DNA short tandem repeat profiling of Morocco.

Jauffrit, Alban / El Amri, Hamid / Airaud, Fabrice / André, Marie-Thérèse / Herbert, Odile / Landeau-Trottier, Gaëlle / Giraudet, Stéphanie / Richard, Christel / Chaventré, André / Moisan, Jean-Paul

Journal of forensic sciences

2003 Volume 48, Issue 2, Page(s) 458–459

MeSH term(s)	DNA Fingerprinting/methods ; Genetics, Population ; Humans ; Male ; Morocco ; Tandem Repeat Sequences
Language	English
Publishing date	2003-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	219216-0
ISSN	1556-4029 ; 0022-1198
ISSN (online)	1556-4029
ISSN	0022-1198
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