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  1. Article ; Online: The alamar blue assay in the context of safety testing of nanomaterials.

    Longhin, Eleonora Marta / El Yamani, Naouale / Rundén-Pran, Elise / Dusinska, Maria

    Frontiers in toxicology

    2022  Volume 4, Page(s) 981701

    Abstract: The Alamar Blue (AB) assay is widely used to investigate cytotoxicity, cell proliferation and cellular metabolic activity within different fields of toxicology. The use of the assay with nanomaterials (NMs) entails specific aspects including the ... ...

    Abstract The Alamar Blue (AB) assay is widely used to investigate cytotoxicity, cell proliferation and cellular metabolic activity within different fields of toxicology. The use of the assay with nanomaterials (NMs) entails specific aspects including the potential interference of NMs with the test. The procedure of the AB assay applied for testing NMs is described in detail and step-by-step, from NM preparation, cell exposure, inclusion of interference controls, to the analysis and interpretation of the results. Provided that the proper procedure is followed, and relevant controls are included, the AB assay is a reliable and high throughput test to evaluate the cytotoxicity/proliferation/metabolic response of cells exposed to NMs.
    Language English
    Publishing date 2022-09-28
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-3080
    ISSN (online) 2673-3080
    DOI 10.3389/ftox.2022.981701
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The colony forming efficiency assay for toxicity testing of nanomaterials-Modifications for higher-throughput.

    Rundén-Pran, Elise / Mariussen, Espen / El Yamani, Naouale / Elje, Elisabeth / Longhin, Eleonora Marta / Dusinska, Maria

    Frontiers in toxicology

    2022  Volume 4, Page(s) 983316

    Abstract: To cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods ... ...

    Abstract To cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods for
    Language English
    Publishing date 2022-09-07
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-3080
    ISSN (online) 2673-3080
    DOI 10.3389/ftox.2022.983316
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Lack of mutagenicity of TiO

    El Yamani, Naouale / Rubio, Laura / García-Rodríguez, Alba / Kažimírová, Alena / Rundén-Pran, Elise / Magdalena, Barančoková / Marcos, Ricard / Dusinska, Maria

    Mutation research. Genetic toxicology and environmental mutagenesis

    2022  Volume 882, Page(s) 503545

    Abstract: The potential genotoxicity of titanium dioxide ( ... ...

    Abstract The potential genotoxicity of titanium dioxide (TiO
    MeSH term(s) Comet Assay ; DNA ; DNA Damage ; Hypoxanthine Phosphoribosyltransferase/genetics ; Metal Nanoparticles/toxicity ; Mutagenicity Tests ; Mutagens/toxicity ; Nanoparticles/toxicity ; Purines ; Titanium/toxicity
    Chemical Substances Mutagens ; Purines ; titanium dioxide (15FIX9V2JP) ; DNA (9007-49-2) ; Titanium (D1JT611TNE) ; Hypoxanthine Phosphoribosyltransferase (EC 2.4.2.8)
    Language English
    Publishing date 2022-09-05
    Publishing country Netherlands
    Document type Journal Article
    ISSN 1879-3592
    ISSN (online) 1879-3592
    DOI 10.1016/j.mrgentox.2022.503545
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  4. Article: The Effect of Time on the Stability of Iron Oxide Nanoparticles in Environmental Acids

    Rabajczyk, Anna / El Yamani, Naouale / Dusinska, Maria

    Water environment research. 2017 May, v. 89, no. 5

    2017  

    Abstract: Advanced technologies seek for development of new materials and substances with extraordinary physicochemical properties at nanoscale level that boosts their increased use in everyday life. Manufacture of metal nanomaterials, including iron, carries the ... ...

    Abstract Advanced technologies seek for development of new materials and substances with extraordinary physicochemical properties at nanoscale level that boosts their increased use in everyday life. Manufacture of metal nanomaterials, including iron, carries the risk of their emission to surface waters. Suspended particulate matter (SPM) plays an important role in the transport of pollutants, such as metals which are an essential component of surface waters. The humic substances (HA), part of the SPM, interact with metal ions present in the aquatic environment. However, the previously available data on these compounds were obtained at the macro level and only scant information exist on nanomaterials. Thus, the present work has focused on the relationship between humic substances and nanosized particles, such as n‐Fe₂O₃, in environmental acids.
    Keywords aquatic environment ; iron ; iron oxides ; manufacturing ; particulates ; research ; risk
    Language English
    Dates of publication 2017-05
    Size p. 416-423.
    Publishing place Water Environment Federation
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 1098976-6
    ISSN 1554-7531 ; 1047-7624 ; 1061-4303
    ISSN (online) 1554-7531
    ISSN 1047-7624 ; 1061-4303
    DOI 10.2175/106143016X14609975747685
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4344: Show issues

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  5. Article: The Effect of Time on the Stability of Iron Oxide Nanoparticles in Environmental Acids.

    Rabajczyk, Anna / El Yamani, Naouale / Dusinska, Maria

    Water environment research : a research publication of the Water Environment Federation

    2017  Volume 89, Issue 5, Page(s) 416–423

    Abstract: Advanced technologies seek for development of new materials and substances with extraordinary physicochemical properties at nanoscale level that boosts their increased use in everyday life. Manufacture of metal nanomaterials, including iron, carries the ... ...

    Abstract Advanced technologies seek for development of new materials and substances with extraordinary physicochemical properties at nanoscale level that boosts their increased use in everyday life. Manufacture of metal nanomaterials, including iron, carries the risk of their emission to surface waters. Suspended particulate matter (SPM) plays an important role in the transport of pollutants, such as metals which are an essential component of surface waters. The humic substances (HA), part of the SPM, interact with metal ions present in the aquatic environment. However, the previously available data on these compounds were obtained at the macro level and only scant information exist on nanomaterials. Thus, the present work has focused on the relationship between humic substances and nanosized particles, such as n-Fe2O3, in environmental acids.
    MeSH term(s) Environment ; Ferric Compounds/chemistry ; Humic Substances/analysis ; Metal Nanoparticles/chemistry ; Time Factors
    Chemical Substances Ferric Compounds ; Humic Substances ; ferric oxide (1K09F3G675)
    Language English
    Publishing date 2017-05-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1098976-6
    ISSN 1554-7531 ; 1061-4303 ; 1047-7624
    ISSN (online) 1554-7531
    ISSN 1061-4303 ; 1047-7624
    DOI 10.2175/106143016X14609975747685
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 490: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Sensitive detection of DNA oxidation damage induced by nanomaterials.

    Collins, Andrew / El Yamani, Naouale / Dusinska, Maria

    Free radical biology & medicine

    2017  Volume 107, Page(s) 69–76

    Abstract: From a toxicological point of view, nanomaterials are of interest; because - on account of their great surface area relative to mass - they tend to be more reactive than the bulk chemicals from which they are derived. They might in some cases have the ... ...

    Abstract From a toxicological point of view, nanomaterials are of interest; because - on account of their great surface area relative to mass - they tend to be more reactive than the bulk chemicals from which they are derived. They might in some cases have the potential to damage DNA directly, or could act via the induction of oxidative stress. The comet assay (single cell gel electrophoresis) is widely used to measure DNA strand breaks and also oxidised bases, by including in the procedure digestion with lesion-specific enzymes such as formamidopyrimidine DNA glycosylase (which converts oxidised purines to breaks) or endonuclease III (recognising oxidised pyrimidines). We summarise reports in which these enzymes have been used to study a variety of nanomaterials in diverse cell types. We also stress that it is important to carry out tests of cell viability alongside the genotoxicity assay, since cytotoxicity can lead to adventitious DNA damage. Different concentrations of nanomaterials should be investigated, concentrating on a non-cytotoxic range; and incubating for short and longer periods can give valuable information about the mode of damage induction. The use of lesion-specific enzymes can substantially enhance the sensitivity of the comet assay in detecting genotoxic effects.
    Language English
    Publishing date 2017-06
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2017.02.001
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2109: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Current status and future challenges of genotoxicity OECD Test Guidelines for nanomaterials: a workshop report.

    Doak, Shareen H / Andreoli, Cristina / Burgum, Michael J / Chaudhry, Qasim / Bleeker, Eric A J / Bossa, Cecilia / Domenech, Josefa / Drobne, Damjana / Fessard, Valérie / Jeliazkova, Nina / Longhin, Eleonora / Rundén-Pran, Elise / Stępnik, Maciej / El Yamani, Naouale / Catalán, Julia / Dusinska, Maria

    Mutagenesis

    2023  Volume 38, Issue 4, Page(s) 183–191

    Abstract: Genotoxicity testing for nanomaterials remains challenging as standard testing approaches require some adaptation, and further development of nano-specific OECD Test Guidelines (TGs) and Guidance Documents (GDs) are needed. However, the field of ... ...

    Abstract Genotoxicity testing for nanomaterials remains challenging as standard testing approaches require some adaptation, and further development of nano-specific OECD Test Guidelines (TGs) and Guidance Documents (GDs) are needed. However, the field of genotoxicology continues to progress and new approach methodologies (NAMs) are being developed that could provide relevant information on the range of mechanisms of genotoxic action that may be imparted by nanomaterials. There is a recognition of the need for implementation of new and/or adapted OECD TGs, new OECD GDs, and utilization of NAMs within a genotoxicity testing framework for nanomaterials. As such, the requirements to apply new experimental approaches and data for genotoxicity assessment of nanomaterials in a regulatory context is neither clear, nor used in practice. Thus, an international workshop with representatives from regulatory agencies, industry, government, and academic scientists was convened to discuss these issues. The expert discussion highlighted the current deficiencies that exist in standard testing approaches within exposure regimes, insufficient physicochemical characterization, lack of demonstration of cell or tissue uptake and internalization, and limitations in the coverage of genotoxic modes of action. Regarding the latter aspect, a consensus was reached on the importance of using NAMs to support the genotoxicity assessment of nanomaterials. Also highlighted was the need for close engagement between scientists and regulators to (i) provide clarity on the regulatory needs, (ii) improve the acceptance and use of NAM-generated data, and (iii) define how NAMs may be used as part of weight of evidence approaches for use in regulatory risk assessments.
    MeSH term(s) Organisation for Economic Co-Operation and Development ; Mutagenicity Tests/methods ; Nanostructures/toxicity ; Nanostructures/chemistry ; Risk Assessment
    Language English
    Publishing date 2023-05-26
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632903-2
    ISSN 1464-3804 ; 0267-8357
    ISSN (online) 1464-3804
    ISSN 0267-8357
    DOI 10.1093/mutage/gead017
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2189: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Rapid identification of in vitro cell toxicity using an electrochemical membrane screening platform.

    Kohl, Yvonne / William, Nicola / Elje, Elisabeth / Backes, Nadine / Rothbauer, Mario / Srancikova, Annamaria / Rundén-Pran, Elise / El Yamani, Naouale / Korenstein, Rafi / Madi, Lea / Barbul, Alexander / Kozics, Katarina / Sramkova, Monika / Steenson, Karen / Gabelova, Alena / Ertl, Peter / Dusinska, Maria / Nelson, Andrew

    Bioelectrochemistry (Amsterdam, Netherlands)

    2023  Volume 153, Page(s) 108467

    Abstract: This study compares the performance and output of an electrochemical phospholipid membrane platform against respective in vitro cell-based toxicity testing methods using three toxicants of different biological action (chlorpromazine (CPZ), colchicine ( ... ...

    Abstract This study compares the performance and output of an electrochemical phospholipid membrane platform against respective in vitro cell-based toxicity testing methods using three toxicants of different biological action (chlorpromazine (CPZ), colchicine (COL) and methyl methanesulphonate (MMS)). Human cell lines from seven different tissues (lung, liver, kidney, placenta, intestine, immune system) were used to validate this physicochemical testing system. For the cell-based systems, the effective concentration at 50 % cell death (EC
    MeSH term(s) Humans ; Cell Line ; Toxicity Tests/methods ; Liver ; Chlorpromazine ; Hazardous Substances ; Phospholipids
    Chemical Substances Chlorpromazine (U42B7VYA4P) ; Hazardous Substances ; Phospholipids
    Language English
    Publishing date 2023-05-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2010650-6
    ISSN 1878-562X ; 0302-4598 ; 1567-5394
    ISSN (online) 1878-562X
    ISSN 0302-4598 ; 1567-5394
    DOI 10.1016/j.bioelechem.2023.108467
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1178: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Fluorescent in situ hybridization on comets: FISH comet.

    Shaposhnikov, Sergey / El Yamani, Naouale / Collins, Andrew R

    Methods in molecular biology (Clifton, N.J.)

    2015  Volume 1288, Page(s) 363–373

    Abstract: The DNA in eukaryotic cells is organized into loop domains that represent basic structural and functional units of chromatin packaging. The comet assay, a sensitive method for monitoring DNA damage and repair, involves electrophoresis of nucleoids ... ...

    Abstract The DNA in eukaryotic cells is organized into loop domains that represent basic structural and functional units of chromatin packaging. The comet assay, a sensitive method for monitoring DNA damage and repair, involves electrophoresis of nucleoids comprising supercoiled DNA attached to the nuclear matrix. Breaks in the DNA relax the supercoiling and allow DNA loops to expand, and on electrophoresis to move towards the anode, giving the appearance of a comet tail. We use fluorescent in situ hybridization (FISH) to investigate the structure of the chromatin within comet preparations and to study specific DNA sequences within comets. In this chapter we describe our FISH comets protocols, deal with some technical questions and outline the theory. FISH with comets should be useful to researchers interested in the structural organization of DNA and chromatin, the localization of DNA damage, and the kinetics of repair of damage.
    MeSH term(s) Comet Assay/methods ; In Situ Hybridization, Fluorescence/methods
    Language English
    Publishing date 2015
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-2474-5_21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Effects of Titanium Dioxide Nanoparticles on the

    Kazimirova, Alena / El Yamani, Naouale / Rubio, Laura / García-Rodríguez, Alba / Barancokova, Magdalena / Marcos, Ricard / Dusinska, Maria

    Nanomaterials (Basel, Switzerland)

    2020  Volume 10, Issue 3

    Abstract: The genotoxicity of anatase/rutile ... ...

    Abstract The genotoxicity of anatase/rutile TiO
    Language English
    Publishing date 2020-03-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano10030465
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