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Article ; Online: Carbapenem Resistance in Gram-Negative Bacteria: A Hospital-Based Study in Egypt.

Elrahem, Amira Abd / El-Mashad, Noha / Elshaer, Mohammed / Ramadan, Hazem / Damiani, Giovanni / Bahgat, Monir / Mercuri, Santo Raffaele / Elemshaty, Wafaa

Medicina (Kaunas, Lithuania)

2023 Volume 59, Issue 2

Abstract: Background and Objectives: ...

Abstract	Background and Objectives:
MeSH term(s)	Humans ; Anti-Bacterial Agents ; beta-Lactamases/genetics ; Carbapenems ; Cross-Sectional Studies ; Egypt ; Escherichia coli ; Gram-Negative Bacteria ; Hospitals, University ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/genetics ; Drug Resistance, Bacterial
Chemical Substances	Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6) ; Carbapenems
Language	English
Publishing date	2023-02-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2188113-3
ISSN	1648-9144 ; 1010-660X
ISSN (online)	1648-9144
ISSN	1010-660X
DOI	10.3390/medicina59020285
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Article: Adherence and Effectiveness of HBV Vaccination among Healthcare Workers in Egypt.

Elshaer, Mohammed / Elsayed, Eman / El-Gilany, Abdel-Hady / El-Mashad, Noha / Mansour, Mostafa

Indian journal of occupational and environmental medicine

2021 Volume 25, Issue 2, Page(s) 91–95

Abstract: Context: Healthcare workers (HCWs) face a potential risk of acquiring different bloodborne pathogens, by occupational exposure to contaminated blood and body fluids. Hepatitis B vaccine is a safe, effective method of conferring long-term protection ... ...

Abstract	Context: Healthcare workers (HCWs) face a potential risk of acquiring different bloodborne pathogens, by occupational exposure to contaminated blood and body fluids. Hepatitis B vaccine is a safe, effective method of conferring long-term protection against HBV infection. Aims: The study aimed to assess the adherence and effectiveness of HBV vaccination among HCWs at Gastrointestinal Surgical Center, Mansoura University, Egypt. Settings and design: A prospective descriptive study was carried out between June 2019 and December 2019 at Gastrointestinal Surgical Center, Mansoura University, Egypt. Methods and material: All HCWs with anti-HBs levels below 10 mIU/mL were advised to receive 3 doses of recombinant HBV vaccine, at 0, 1, and 6 months. The anti-HBs levels were checked 3 months after the third dose of the HBV vaccine. Statistical analysis used: Data was analyzed using the Statistical Package of Social Science (SPSS) program for windows (version 16). Results: A total of 442 healthcare providers were included. Most of them completed the 3 doses of the vaccine (81.7%), 10.2% refused the vaccine, while 0.9% and 7.2% received 1 and 2 doses, respectively. Odds of vaccination were the highest (88.1%, 273/310) among nurses (OR, 4.7; 95% CI, 2.6-5.2; Conclusions: The outcome of the present study emphasizes the need to apply alternative and innovative measures to build a positive attitude toward the HBV vaccine among HCWs.
Language	English
Publishing date	2021-07-09
Publishing country	India
Document type	Journal Article
ZDB-ID	1456082-3
ISSN	1998-3670 ; 0973-2284 ; 0019-5278
ISSN (online)	1998-3670
ISSN	0973-2284 ; 0019-5278
DOI	10.4103/ijoem.IJOEM_139_20
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Article ; Online: Extensive drug resistant Acinetobacter baumannii: a comparative study between non-colistin based combinations.

Elsayed, Eman / Elarabi, Mohamed A / Sherif, Dana A / Elmorshedi, Mohamed / El-Mashad, Noha

International journal of clinical pharmacy

2019 Volume 42, Issue 1, Page(s) 80–88

Abstract: Background The Gastrointestinal Surgery Center (GISC)-Mansoura University, faced a series of extensive drug resistant (XDR) A. baumannii cases, that were microbiologically resistant to penicillins, cephalosporins, fluoroquinolones, aminoglycosides, ... ...

Abstract	Background The Gastrointestinal Surgery Center (GISC)-Mansoura University, faced a series of extensive drug resistant (XDR) A. baumannii cases, that were microbiologically resistant to penicillins, cephalosporins, fluoroquinolones, aminoglycosides, carbapenems and tigecycline. Colistin would have been a last resort therapy in such situation, however, intravenous polymyxins E (colistin) is relatively unavailable in Egypt. Many practitioners tried to form antibiotic combinations from the available antibiotics to overcome the resistance mechanisms of the pathogen. Objective Evaluate the clinical outcomes of these combinations retrospectively. Setting The study took place at the GISC, which is an academic specialized center affiliated with Mansoura University-Egypt. Method Clinical data were collected from the patients' files, where the subjects were classified into two major groups according to the therapeutic intervention. Group 1 included 24 patients divided into 4 subgroups. The first was treated by a Cephalosporin with a Fluoroquinolone (1A), The second was treated by a Carbapenem with a Fluoroquinolone (1B), The third was treated by a B-lactam with an Aminoglycoside (1C) and the fourth was treated by Carbapenem with a Glycylcycline (1D). Group 2 included 6 patients, treated with Tigecycline and Ampicillin-Sulbactam. Main outcome measure Primary outcomes are the A. baumannii microbiological culture negativity after 14 days of therapy and the 30 days' survival after the antibiotic course, while the secondary outcomes are the expected therapies' side effects. Results Group 2 is associated with significant higher primary outcomes without a significant difference regarding the secondary outcomes. Conclusion The combination of Tigecycline and Ampicillin-Sulbactam, appears to be a clinically effective therapy against XDR A. baumannii, despite each agent being resistant alone, without alerting adverse effects.
MeSH term(s)	Acinetobacter Infections/drug therapy ; Acinetobacter baumannii/drug effects ; Acinetobacter baumannii/isolation & purification ; Acinetobacter baumannii/physiology ; Adult ; Aged ; Ampicillin/administration & dosage ; Anti-Bacterial Agents/administration & dosage ; Colistin ; Drug Resistance, Multiple, Bacterial/drug effects ; Drug Resistance, Multiple, Bacterial/physiology ; Drug Therapy, Combination ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Sulbactam/administration & dosage ; Tigecycline/administration & dosage
Chemical Substances	Anti-Bacterial Agents ; sultamicillin (65DT0ML581) ; Tigecycline (70JE2N95KR) ; Ampicillin (7C782967RD) ; Sulbactam (S4TF6I2330) ; Colistin (Z67X93HJG1)
Language	English
Publishing date	2019-11-19
Publishing country	Netherlands
Document type	Comparative Study ; Journal Article
ZDB-ID	2601204-2
ISSN	2210-7711 ; 2210-7703 ; 0928-1231
ISSN (online)	2210-7711
ISSN	2210-7703 ; 0928-1231
DOI	10.1007/s11096-019-00940-1
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Article ; Online: An overview of healthcare-associated infections in a tertiary care hospital in Egypt.

Hassan, Rania / El-Gilany, Abdel-Hady / Abd Elaal, Amina M / El-Mashad, Noha / Azim, Dalia Abdel

Infection prevention in practice

2020 Volume 2, Issue 3, Page(s) 100059

Abstract: Background: Healthcare-associated infection (HAI) is a major problem in healthcare facilities and is associated with increased morbidity and mortality and prolonged hospital stay. This study aims to determine the incidence rate, risk factors, and ... ...

Abstract	Background: Healthcare-associated infection (HAI) is a major problem in healthcare facilities and is associated with increased morbidity and mortality and prolonged hospital stay. This study aims to determine the incidence rate, risk factors, and bacterial aetiology of HAI in a tertiary care hospital in Mansoura, Egypt. Methods: This is a prospective observational study carried out over 12 months in different departments of Mansoura New General Hospital (MNGH). Data were collected from patient's records and laboratory results of the ongoing HAI surveillance program. Results: The incidence of HAI was 3.7% among 6912 patients studied. The independent predictors of HAI were multiple devices (AOR=88.1), central venous catheter (CVC) (AOR=34), urinary catheter (AOR=28.9) and length of stay >20 days (AOR=3.1). Surgical site infections (SSI) were the most frequent (24%) followed by catheter associated urinary tract infections (CAUTI) (20%). The most frequently isolated pathogens were Conclusions: HAI is a significant problem in MNGH.
Language	English
Publishing date	2020-05-19
Publishing country	England
Document type	Journal Article
ISSN	2590-0889
ISSN (online)	2590-0889
DOI	10.1016/j.infpip.2020.100059
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Article: TNF-α gene polymorphisms and expression.

El-Tahan, Radwa R / Ghoneim, Ahmed M / El-Mashad, Noha

SpringerPlus

2016 Volume 5, Issue 1, Page(s) 1508

Abstract: Tumor necrosis factor alpha (TNF-α) is a proinflammatory cytokine with an important role in the pathogenesis of several diseases. Its encoding gene is located in the short arm of chromosome 6 in the major histocompatibility complex class III region. Most ...

Abstract	Tumor necrosis factor alpha (TNF-α) is a proinflammatory cytokine with an important role in the pathogenesis of several diseases. Its encoding gene is located in the short arm of chromosome 6 in the major histocompatibility complex class III region. Most of the TNF-α gene polymorphisms are located in its promoter region and they are thought to affect the susceptibility and/or severity of different human diseases. This review summarizes the data related to the association between TNF-α gene and its receptor polymorphisms, and the development of autoimmune diseases. Among these polymorphisms the -308G/A TNF-α promotor polymorphism has been associated several times with the the development of autoimmune diseases, however some discrepant results have been recorded. The other TNF-α gene polymorphisms had little or no association with autoimmune diseases. Current results about the molecules controlling TNF-α expression are also presented. The discrepancy between different records could be related partly to either the differences in the ethnic origin or number of the studied individuals, or the abundance and activation of other molecules that interact with the TNF-α promotor region or other elements.
Language	English
Publishing date	2016-09-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2661116-8
ISSN	2193-1801
ISSN	2193-1801
DOI	10.1186/s40064-016-3197-y
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Article ; Online: A study of the treatment of cutaneous fungal infection in animal model using photoactivated composite of methylene blue and gold nanoparticle.

Tawfik, Abeer Attia / Noaman, Islam / El-Elsayyad, Hasan / El-Mashad, Noha / Soliman, Mona

Photodiagnosis and photodynamic therapy

2016 Volume 15, Page(s) 59–69

Abstract: Background: Onychomycosis is a widespread public health problem, in which T. rubrum and T. mentagrophytes is the commenest causative organisms. Current medical therapy has many drawbacks and side effects. Methylene blue (m.b) photodynamic therapy (pdt) ... ...

Abstract	Background: Onychomycosis is a widespread public health problem, in which T. rubrum and T. mentagrophytes is the commenest causative organisms. Current medical therapy has many drawbacks and side effects. Methylene blue (m.b) photodynamic therapy (pdt) proved efficacy but with lengthy sessions. Objectives: Optimizing methylene blue photodynamic therapy by combination of methylene blue photosensitizer and gold nanoparticles (aunps) in a composite as gold nanoparticles are efficient delivery systems and efficient enhancers of photosensitizers for antifungal photodynamic therapy. Materials and methods: Eighty newzealand rabbit (Oryctolagus cuniculus) were used and categorized in eight equal groups as follows; healthy and infection control, composite photodynamic therapy and five comparative groups. Photodynamic therapy was initiated at day three to five post inoculation, for four sessions forty eight hours apart. Each group divided and light exposure at two fluencies; 80J and 100J. All groups were investigated macroscopically and microscopically (histopathology and scanning electron microscope) also flowcytometry assessment for cell death and X-ray analysis for gold nanoparticles accumulation in brain and liver tissues were determined. Results: Recovery from infection approaching 96% in gold nanoparticles+light group, around 40% in methylene blue photodynamic therapy and 34% in composite photodynamic therapy. The observed findings confirmed by apparent decrease of apoptosis, however small amounts of gold nanoparticles detected in brain and liver. Conclusion: Light stimulated gold nanoparticles is a promising tool in treatment of onychomycosis.
MeSH term(s)	Animals ; Dose-Response Relationship, Drug ; Dose-Response Relationship, Radiation ; Drug Therapy, Combination/methods ; Gold/administration & dosage ; Gold/chemistry ; Humans ; Light ; Metal Nanoparticles/administration & dosage ; Metal Nanoparticles/chemistry ; Metal Nanoparticles/ultrastructure ; Methylene Blue/administration & dosage ; Nanocomposites/administration & dosage ; Nanocomposites/chemistry ; Nanocomposites/ultrastructure ; Onychomycosis/drug therapy ; Onychomycosis/microbiology ; Onychomycosis/pathology ; Particle Size ; Photochemotherapy/methods ; Photosensitizing Agents/administration & dosage ; Rabbits ; Tinea/drug therapy ; Tinea/microbiology ; Tinea/pathology ; Treatment Outcome
Chemical Substances	Photosensitizing Agents ; Gold (7440-57-5) ; Methylene Blue (T42P99266K)
Language	English
Publishing date	2016-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2149918-4
ISSN	1873-1597 ; 1572-1000
ISSN (online)	1873-1597
ISSN	1572-1000
DOI	10.1016/j.pdpdt.2016.05.010
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Article ; Online: Does the serum testosterone level have a relation to coronary artery disease in elderly men?

Helaly, Mohamed A / Daoud, Eid / El-Mashad, Noha

Current gerontology and geriatrics research

2011 Volume 2011, Page(s) 791765

Abstract: Background. The low serum level of testosterone in the elderly subjects may contribute to coronary artery disease (CAD). Our aim is to study serum levels of free testosterone in elderly men with CAD. Subjects and Methods. This study was conducted on 100 ... ...

Abstract	Background. The low serum level of testosterone in the elderly subjects may contribute to coronary artery disease (CAD). Our aim is to study serum levels of free testosterone in elderly men with CAD. Subjects and Methods. This study was conducted on 100 elderly males with CAD, one half of them was presented with ACS (with mean age 69.6 ± 2.44 year), and the other half was presented with stable CAD (with mean age 69.42 ± 2.14 year), in addition to 50 apparently healthy elderly males (with mean age 69.06 ± 1.64 year) as a control group. We detected the levels of serum free testosterone, cortisol, fibrinogen, plasminogen activator inhibitor-1(PAI-1), high sensitive C-reactive protein(hsCRP), interleukin-6(IL-6). Results. Cases with CAD had significant lower values of free testosterone and HDL-c, but they had significant higher values of cortisol, fibrinogen, PAI-1, IL-6, hsCRP, in comparison to control group. Cases with ACS had significant higher values of cortisol, hsCRP, IL-6, fibrinogen, PAI-1, total cholesterol and BMI more than those with stable CAD. The free testosterone had significant negative correlation with fibrinogen, PAI-1, hsCRP and IL-6 in both groups of patients. Conclusion. The lower value of serum free testosterone in elderly male subjects may contribute to CAD.
Language	English
Publishing date	2011-05-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2475149-2
ISSN	1687-7071 ; 1687-7063
ISSN (online)	1687-7071
ISSN	1687-7063
DOI	10.1155/2011/791765
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Article: Susceptibility tests of oropharyngeal Candida albicans from egyptian patients to fluconazole determined by three methods.

El-Mashad, Noha / Foad, Mona F / Saudy, Niveen / Salem, Dalia A

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

2012 Volume 43, Issue 1, Page(s) 266–273

Abstract: Candida albicans frequently cause oropharyngeal candidiasis in immunocompromised patients. As some of these isolates show resistance against azoles, the clinician is wary of initiating therapy with fluconazole (FZ) until a final susceptibility report is ... ...

Abstract	Candida albicans frequently cause oropharyngeal candidiasis in immunocompromised patients. As some of these isolates show resistance against azoles, the clinician is wary of initiating therapy with fluconazole (FZ) until a final susceptibility report is generated. We aimed to evaluate the efficacy of rapid flow cytometry (FCM) and disc diffusion (DD) methods in comparison to reference microdilution (MD) of Clinical and Laboratory Standards Institute (CLSI) method for FZ. Thirty seven Candida albicans isolates were tested by the three methods. By both MD and FCM, 26/37 (70.3%) were sensitive with minimal inhibitory concentration (MIC) ≤ 8μg/ml, 5/37 (13.5%) were susceptible dose dependant (S-DD) with MIC 16-32 μg/ml and 6/37 (16.2%) were resistant with MIC ≥64μg/ml. More than 92% of isolates susceptible to FZ by the MD were susceptible by the DD methods with good agreement (81.08%, P = 0.000). However, 4/5 isolates diagnosed as S-DD by MD were resistant by DD. Interestingly, the MIC by FCM at 4 h showed excellent agreement (95.59%, P = 0.000) to that obtained by MD method at 24 h. Overall, FCM antifungal susceptibility testing provided rapid, reproducible results that are valuable alternative to MD. The DD test is recommended as a simple and reliable screening test for the detection of susceptible Candida albicans isolates to FZ.
Language	English
Publishing date	2012-06-01
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2042078-X
ISSN	1517-8382
ISSN	1517-8382
DOI	10.1590/S1517-838220120001000031
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Article ; Online: Genotypic detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains by DNA sequencing

El mashad Noha / Zaghloul Mohammad / El-Ghaffar Hassan / Abdelaal Amina / Badran Ehab / Fathy Amal

Annals of Clinical Microbiology and Antimicrobials, Vol 8, Iss 1, p

a randomized trial

2009 Volume 4

Abstract: Abstract Background Tuberculosis is a growing international health concern. It is the biggest killer among the infectious diseases in the world today. Early detection of drug resistance allows starting of an appropriate treatment. Resistance to drugs is ... ...

Abstract	Abstract Background Tuberculosis is a growing international health concern. It is the biggest killer among the infectious diseases in the world today. Early detection of drug resistance allows starting of an appropriate treatment. Resistance to drugs is due to particular genomic mutations in specific genes of Mycobacterium tuberculosis(MTB). The aim of this study was to identify the presence of Isoniazid (INH) and Rifampicin(RIF) drug resistance in new and previously treated tuberculosis (TB) cases using DNA sequencing. Methods This study was carried out on 153 tuberculous patients with positive Bactec 460 culture for acid fast bacilli. Results Of the 153 patients, 105 (68.6%) were new cases and 48 (31.4%) were previously treated cases. Drug susceptibility testing on Bactec revealed 50 resistant cases for one or more of the first line antituberculous. Genotypic analysis was done only for rifampicin resistant specimens (23 cases) and INH resistant specimens (26 cases) to detect mutations responsible for drug resistance by PCR amplification of rpoB gene for rifampicin resistant cases and KatG gene for isoniazid resistant cases. Finally, DNA sequencing was done for detection of mutation within rpoB and KatG genes. Genotypic analysis of RIF resistant cases revealed that 20/23 cases (86.9%) of RIF resistance were having rpoB gene mutation versus 3 cases (13.1%) having no mutation with a high statistical significant difference between them (P < 0.001). Direct sequencing of Kat G gene revealed point mutation in 24/26 (92.3%) and the remaining 2/26 (7.7%) had wild type KatG i.e. no evidence of mutation with a high statistical significant difference between them (P < 0.001). Conclusion We can conclude that rifampicin resistance could be used as a useful surrogate marker for estimation of multidrug resistance. In addition, Genotypic method was superior to that of the traditional phenotypic method which is time-consuming taking several weeks or longer.
Keywords	Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Subject code	630
Language	English
Publishing date	2009-01-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
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Article ; Online: Genotypic detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains by DNA sequencing

El mashad Noha / Zaghloul Mohammad / El-Ghaffar Hassan / Abdelaal Amina / Badran Ehab / Fathy Amal

Annals of Clinical Microbiology and Antimicrobials, Vol 8, Iss 1, p

a randomized trial

2009 Volume 5

Abstract: Abstract Correction to Genotypic detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains by DNA sequencing: a randomized trial Amina Abdelaal, Hassan Abd El-Ghaffar, Mohammad Hosam Eldeen Zaghloul, Noha El mashad, Ehab Badran, ... ...

Abstract	Abstract Correction to Genotypic detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains by DNA sequencing: a randomized trial Amina Abdelaal, Hassan Abd El-Ghaffar, Mohammad Hosam Eldeen Zaghloul, Noha El mashad, Ehab Badran, Amal Fathy Annals of Clinical Microbiology and Antimicrobials 2009, 8:4 (30 January 2009)
Keywords	Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Therapeutics. Pharmacology ; RM1-950 ; Infectious and parasitic diseases ; RC109-216
Language	English
Publishing date	2009-02-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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