Article ; Online: Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis.
2019 Volume 36, Issue 1, Page(s) 39–55
Abstract: Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. However, the mechanisms underlying such properties have only been partially elucidated. In this study, we isolated and purified a cell-wall associated ... ...
Abstract | Lactobacillus farciminis CIP 103136 is a bacterial strain with recognized probiotic properties. However, the mechanisms underlying such properties have only been partially elucidated. In this study, we isolated and purified a cell-wall associated polysaccharide (CWPS), and evaluated its biological role in vitro. The structure of CWPS and responses from stimulation of (i) human macrophage-like THP-1 cells, (ii) human embryonal kidney (HEK293) cells stably transfected with Toll-like receptors (TLR2 or TLR4) and (iii) human colonocyte-like T84 intestinal epithelial cells, upon exposure to CWPS were studied. The structure of the purified CWPS from L. farciminis CIP 103136 was analyzed by nuclear magnetic resonance (NMR), MALDI-TOF-TOF MS, and methylation analyses in its native form and following Smith degradation. It was shown to be a novel branched polysaccharide, composed of linear backbone of trisaccharide repeating units of: [→6αGlcpNAc1 → 4βManpNAc1 → 4βGlcpNAc1→] highly substituted with single residues of αGlcp, αGalp and αGlcpNAc. Subsequently, the lack of pro- or anti-inflammatory properties of CWPS was established on macrophage-like THP-1 cells. In addition, CWPS failed to modulate cell signaling pathways dependent of TLR2 and TLR4 in transfected HEK-cells. Finally, in T84 cells, CWPS neither influenced intestinal barrier integrity under basal conditions nor prevented TNF-α/IFN-γ cytokine-mediated epithelium impairment. |
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MeSH term(s) | Cell Wall/chemistry ; Cell Wall/ultrastructure ; Cytokines/metabolism ; HEK293 Cells ; Hexosamines/analysis ; Humans ; Lactobacillus/chemistry ; Polysaccharides, Bacterial/chemistry ; Polysaccharides, Bacterial/immunology ; Polysaccharides, Bacterial/pharmacology ; Probiotics/chemistry ; Signal Transduction/immunology ; Toll-Like Receptors/metabolism |
Chemical Substances | Cytokines ; Hexosamines ; Polysaccharides, Bacterial ; Toll-Like Receptors |
Language | English |
Publishing date | 2019-01-12 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 283770-5 |
ISSN | 1573-4986 ; 0282-0080 |
ISSN (online) | 1573-4986 |
ISSN | 0282-0080 |
DOI | 10.1007/s10719-018-09854-y |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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