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Article ; Online: Modelling of technical, environmental, and economic evaluations of the effect of the organic loading rate in semi-continuous anaerobic digestion of pre-treated organic fraction municipal solid waste.

Francesca, Demichelis / Elisa, Robotti / Alessandro, Deorsola Fabio / Emilio, Marengo / Tonia, Tommasi / Debora, Fino

Environmental pollution (Barking, Essex : 1987)

2024 Volume 344, Page(s) 123417

Abstract: The study concerned technical feasibility, economic profitability, and carbon footprint (CF) analysis of semi-continuous anaerobic digestion (sAD) of organic fraction of municipal solid waste (OFMSW). The research assessed the pre-treatment effect on sAD ...

Abstract	The study concerned technical feasibility, economic profitability, and carbon footprint (CF) analysis of semi-continuous anaerobic digestion (sAD) of organic fraction of municipal solid waste (OFMSW). The research assessed the pre-treatment effect on sAD by varying organic loading rates (OLR) from 3.38 to 6.75 kgvs/m
MeSH term(s)	Cost-Benefit Analysis ; Solid Waste ; Anaerobiosis ; Algorithms ; Hydrodynamics ; Bioreactors ; Refuse Disposal ; Methane
Chemical Substances	Solid Waste ; Methane (OP0UW79H66)
Language	English
Publishing date	2024-01-20
Publishing country	England
Document type	Journal Article
ZDB-ID	280652-6
ISSN	1873-6424 ; 0013-9327 ; 0269-7491
ISSN (online)	1873-6424
ISSN	0013-9327 ; 0269-7491
DOI	10.1016/j.envpol.2024.123417
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: On the Traceability of the Hazelnut Production Chain by Means of Trace Elements

Elisa Calà / Andrea Fracchia / Elisa Robotti / Federica Gulino / Francesca Gullo / Matteo Oddone / Marco Massacane / Gianluigi Cordone / Maurizio Aceto

Molecules, Vol 27, Iss 3854, p

2022 Volume 3854

Abstract: The production chain of hazelnuts has been studied by analyzing three sets of samples produced in purity from three different pools of hazelnuts of cultivar “Tonda Gentile Trilobata”, “Tonda Gentile Romana” and “Mortarella”, all cultivated in Italy. From ...

Abstract	The production chain of hazelnuts has been studied by analyzing three sets of samples produced in purity from three different pools of hazelnuts of cultivar “Tonda Gentile Trilobata”, “Tonda Gentile Romana” and “Mortarella”, all cultivated in Italy. From each pool, five processed products were obtained: roasted hazelnuts, hazelnut paste, hazelnut cream, Gianduja paste and Gianduiotto paste. After pre-treatment by means of dry ashing, all samples from each cultivar, including raw hazelnuts, were then analyzed by means of Inductively Coupled Plasma–Optical Emission Spectroscopy (ICP-OES) and Inductively Coupled Plasma–Mass Spectrometry (ICP-MS). A good discrimination was obtained among the different chain stages according to the distribution of the trace elements, as expected. More interesting was the discrimination among the different cultivars: it was possible to distinguish the samples produced from the respective cultivar by means of specific chemical markers, particularly Mo and Ni.
Keywords	ICP-MS ; traceability ; hazelnuts ; PCA ; production chain ; Organic chemistry ; QD241-441
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Photodegradation of the pure and formulated alpha-cypermethrin insecticide gives different products

Gosetti, Fabio / Bianca Bolfi / Elisa Robotti / Emilio Marengo / Marcello Manfredi / Ugo Chiuminatto

Environmental chemistry letters. 2018 June, v. 16, no. 2

2018

Abstract: Alpha-cypermethrin is a broad-spectrum insecticide widely used in the treatment of rice crops, mainly commercialized as a CONTEST® formulation. The photodegradation of alpha-cypermethrin and of the commercial formulation has not yet been systematically ... ...

Abstract	Alpha-cypermethrin is a broad-spectrum insecticide widely used in the treatment of rice crops, mainly commercialized as a CONTEST® formulation. The photodegradation of alpha-cypermethrin and of the commercial formulation has not yet been systematically investigated in paddy water under natural conditions. Here, paddy water solutions of alpha-cypermethrin and CONTEST® formulation at 5.0 mg L−1 were irradiated under simulated sunlight for 10 days. Hydrolysis experiments were carried out on the same solutions preserved in the dark. Analysis by ultra-high-performance liquid chromatography–tandem mass spectrometry was developed for the identification of photodegradation products. Results show that degradation of pure alpha-cypermethrin and the formulation counterpart produces both common and different photodegradation products. Five out eleven photodegradation products were identified for the first time, in particular three in the alpha-cypermethrin paddy water solution and four in the formulation. Our findings underline the importance of carrying out photodegradation experiments directly on the commercial formulation, since degradation products could be different from the pure insecticide.
Keywords	crops ; cypermethrin ; hydrolysis ; lighting ; liquid chromatography ; paddies ; photolysis ; rice ; tandem mass spectrometry
Language	English
Dates of publication	2018-06
Size	p. 581-590.
Publishing place	Springer International Publishing
Document type	Article
ZDB-ID	2107984-5
ISSN	1610-3661 ; 1610-3653
ISSN (online)	1610-3661
ISSN	1610-3653
DOI	10.1007/s10311-017-0685-4
Database	NAL-Catalogue (AGRICOLA)

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Article: A hydrophilic interaction liquid chromatography–tandem mass spectrometry method for the identification and determination of cationic starch derivatives in air samples

Gosetti, Fabio / Eleonora Mazzucco / Andrea Secco / Elisa Robotti / Marcello Manfredi / Emilio Marengo

Analytical methods. 2017 Mar. 9, v. 9, no. 10

2017

Abstract: The industrial processes of starch cationization are widely used and the workers must protect their health from the potential presence of toxic species, such as 2,3-epoxypropyltrimethylammonium chloride (ETA or Quab 151®) or 3-chloro-2- ... ...

Abstract	The industrial processes of starch cationization are widely used and the workers must protect their health from the potential presence of toxic species, such as 2,3-epoxypropyltrimethylammonium chloride (ETA or Quab 151®) or 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA or Quab 188®) used during the industrial processes. In this study, a sensitive and fast chromatographic method for the identification and determination of these toxic species in samples placed in fixed air sampling positions near to the reactor of starch processing has been developed and fully validated. The method is based on hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry detection. The limits of detection are 3.0 ng mL⁻¹ and 1.0 ng mL⁻¹ for ETA and CTA, respectively, and the RSD% of the intraday (n = 5) and the interday (n = 35) precision of concentration determination are lower than 3.4% and 6.1%, respectively. The solid–liquid extraction procedure employed here is very simple (sonication) and fast (5 min) and allows obtaining reliable recovery values greater than 91.0%. No evidence of statistically significant matrix effects (at a 95% of confidence level) during the mass spectrometry analysis has been found. The method has been successfully applied to filter samples used in routine controls for the safety of workers involved in these type of industrial processes.
Keywords	air ; cationization ; detection limit ; hydrophilic interaction chromatography ; quaternary ammonium compounds ; sampling ; sonication ; starch ; tandem mass spectrometry ; toxicity ; worker honey bees
Language	English
Dates of publication	2017-0309
Size	p. 1634-1642.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/c6ay03409h
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Direct Analysis in Real Time Mass Spectrometry for the Nondestructive Investigation of Conservation Treatments of Cultural Heritage

Marcello Manfredi / Elisa Robotti / Greg Bearman / Fenella France / Elettra Barberis / Pnina Shor / Emilio Marengo

Journal of Analytical Methods in Chemistry, Vol

2016 Volume 2016

Abstract: Today the long-term conservation of cultural heritage is a big challenge: often the artworks were subjected to unknown interventions, which eventually were found to be harmful. The noninvasive investigation of the conservation treatments to which they ... ...

Abstract	Today the long-term conservation of cultural heritage is a big challenge: often the artworks were subjected to unknown interventions, which eventually were found to be harmful. The noninvasive investigation of the conservation treatments to which they were subjected to is a crucial step in order to undertake the best conservation strategies. We describe here the preliminary results on a quick and direct method for the nondestructive identification of the various interventions of parchment by means of direct analysis in real time (DART) ionization and high-resolution time-of-flight mass spectrometry and chemometrics. The method has been developed for the noninvasive analysis of the Dead Sea Scrolls, one of the most important archaeological discoveries of the 20th century. In this study castor oil and glycerol parchment treatments, prepared on new parchment specimens, were investigated in order to evaluate two different types of operations. The method was able to identify both treatments. In order to investigate the effect of the ion source temperature on the mass spectra, the DART-MS analysis was also carried out at several temperatures. Due to the high sensitivity, simplicity, and no sample preparation requirement, the proposed analytical methodology could help conservators in the challenging analysis of unknown treatments in cultural heritage.
Keywords	Analytical chemistry ; QD71-142
Subject code	930
Language	English
Publishing date	2016-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Optimization of the Extraction of the Volatile Fraction from Honey Samples by SPME-GC-MS, Experimental Design, and Multivariate Target Functions

Elisa Robotti / Federica Campo / Marta Riviello / Marco Bobba / Marcello Manfredi / Eleonora Mazzucco / Fabio Gosetti / Giorgio Calabrese / Emanuele Sangiorgi / Emilio Marengo

Journal of Chemistry, Vol

2017 Volume 2017

Abstract: Head space (HS) solid phase microextraction (SPME) followed by gas chromatography with mass spectrometry detection (GC-MS) is the most widespread technique to study the volatile profile of honey samples. In this paper, the experimental SPME conditions ... ...

Abstract	Head space (HS) solid phase microextraction (SPME) followed by gas chromatography with mass spectrometry detection (GC-MS) is the most widespread technique to study the volatile profile of honey samples. In this paper, the experimental SPME conditions were optimized by a multivariate strategy. Both sensitivity and repeatability were optimized by experimental design techniques considering three factors: extraction temperature (from 50°C to 70°C), time of exposition of the fiber (from 20 min to 60 min), and amount of salt added (from 0 to 27.50%). Each experiment was evaluated by Principal Component Analysis (PCA) that allows to take into consideration all the analytes at the same time, preserving the information about their different characteristics. Optimal extraction conditions were identified independently for signal intensity (extraction temperature: 70°C; extraction time: 60 min; salt percentage: 27.50% w/w) and repeatability (extraction temperature: 50°C; extraction time: 60 min; salt percentage: 27.50% w/w) and a final global compromise (extraction temperature: 70°C; extraction time: 60 min; salt percentage: 27.50% w/w) was also reached. Considerations about the choice of the best internal standards were also drawn. The whole optimized procedure was than applied to the analysis of a multiflower honey sample and more than 100 compounds were identified.
Keywords	Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: IEF peptide fractionation method combined to shotgun proteomics enhances the exploration of rice milk proteome

Manfredi, Marcello / Jessica Brandi / Eleonora Conte / Paolo Pidutti / Fabio Gosetti / Elisa Robotti / Emilio Marengo / Daniela Cecconi

Analytical biochemistry. 2017 Nov. 15, v. 537

2017

Abstract: We conducted a proteomics study in order to detect the proteomic method which provides the most complete characterization of the proteins of rice milk. In particular, we compared the results obtained from LC-MS/MS after protein precipitation with acetone ...

Abstract	We conducted a proteomics study in order to detect the proteomic method which provides the most complete characterization of the proteins of rice milk. In particular, we compared the results obtained from LC-MS/MS after protein precipitation with acetone or TCA, as well as the results obtained from LC-MS/MS after protein prefractionation based on SDS-PAGE (GeLC-MS/MS) or ProteoMinerâ¢ technology (ProteoMiner-LC-MS/MS), and after peptide prefractionation based on IEF (pIEF-LC-MS/MS). A total of 158 protein species have been detect in rice milk. The physical-chemical analysis and classification of the identified proteins were also reported. In particular, we showed that pIEF-LC-MS/MS method led to a significant increase in the proteome coverage, allowing the identification of a total of 96 proteins of milk rice. This study demonstrates the utility of a prefractionation step based on pIEF before the shotgun proteomic analysis and offers an in-depth insight into the rice milk proteome.
Keywords	acetone ; fractionation ; polyacrylamide gel electrophoresis ; proteins ; proteome ; proteomics ; rice milk
Language	English
Dates of publication	2017-1115
Size	p. 72-77.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	1110-1
ISSN	1096-0309 ; 0003-2697
ISSN (online)	1096-0309
ISSN	0003-2697
DOI	10.1016/j.ab.2017.08.021
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effects of Nickel, Chlorpyrifos and Their Mixture on the Dictyostelium discoideum Proteome

Francesco Marsano / Aldo Viarengo / Emilio Marengo / Elisa Robotti / Lara Boatti

International Journal of Molecular Sciences, Vol 13, Iss 12, Pp 15679-

2012 Volume 15705

Abstract: Mixtures of chemicals can have additive, synergistic or antagonistic interactions. We investigated the effects of the exposure to nickel, the organophosphate insecticide chlorpyrifos at effect concentrations (EC) of 25% and 50% and their binary mixture ( ... ...

Abstract	Mixtures of chemicals can have additive, synergistic or antagonistic interactions. We investigated the effects of the exposure to nickel, the organophosphate insecticide chlorpyrifos at effect concentrations (EC) of 25% and 50% and their binary mixture (Ec25 + EC25) on Dictyostelium discoideum amoebae based on lysosomal membrane stability (LMS). We treated D. discoideum with these compounds under controlled laboratory conditions and evaluated the changes in protein levels using a two-dimensional gel electrophoresis (2DE) proteomic approach. Nickel treatment at EC25 induced changes in 14 protein spots, 12 of which were down-regulated. Treatment with nickel at EC50 resulted in changes in 15 spots, 10 of which were down-regulated. Treatment with chlorpyrifos at EC25 induced changes in six spots, all of which were down-regulated; treatment with chlorpyrifos at EC50 induced changes in 13 spots, five of which were down-regulated. The mixture corresponding to EC25 of each compound induced changes in 19 spots, 13 of which were down-regulated. The data together reveal that a different protein expression signature exists for each treatment, and that only a few proteins are modulated in multiple different treatments. For a simple binary mixture, the proteomic response does not allow for the identification of each toxicant. The protein spots that showed significant differences were identified by mass spectrometry, which revealed modulations of proteins involved in metal detoxification, stress adaptation, the oxidative stress response and other cellular processes.
Keywords	Dictyostelium discoideum ; toxicity ; nickel ; chlorpyrifos ; proteomics ; mass spectrometry ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	500
Language	English
Publishing date	2012-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Haemolymph from Mytilus galloprovincialis: Response to copper and temperature challenges studied by 1H-NMR metabonomics

Digilio, Giuseppe / Aldo Viarengo / Caterina Oliveri / Claudio Cassino / Davide Musso / Domenico Osella / Elisa Robotti / Emilio Marengo / Susanna Sforzini

Comparative biochemistry and physiology. 2016 May, June, v. 183-184

2016

Abstract: Numerous studies on molluscs have been carried out to clarify the physiological roles of haemolymph serum proteins and haemocytes. However, little is known about the presence and functional role of the serum metabolites. In this study, Nuclear Magnetic ... ...

Abstract	Numerous studies on molluscs have been carried out to clarify the physiological roles of haemolymph serum proteins and haemocytes. However, little is known about the presence and functional role of the serum metabolites. In this study, Nuclear Magnetic Resonance (NMR) was used to assess whether changes of the metabolic profile of Mytilus galloprovincialis haemolymph may reflect alterations of the physiological status of the organisms due to environmental stressors, namely copper and temperature. Mussel haemolymph was taken from the posterior adductor muscle after a 4-day exposure to ambient (16°C) or high temperature (24°C) and in the absence or presence (5μg/L, 20μg/L, or 40μg/L) of sublethal copper (Cu2+). The total glutathione (GSH) concentration in the haemolymph of both control and treated mussels was minimal, indicating the absence of significant contaminations by muscle intracellular metabolites due to the sampling procedure. In the 1H-NMR spectrum of haemolymph, 27 metabolites were identified unambiguously. The separate and combined effects of exposure to copper and temperature on the haemolymph metabolic profile were assessed by Principal Component Analysis (PCA) and Ranking-PCA multivariate analysis. Changes of the metabolomic profile due to copper exposure at 16°C became detectable at a dose of 20μg/L copper. Alanine, lysine, serine, glutamine, glycogen, glucose and protein aliphatics played a major role in the classification of the metabolic changes according to the level of copper exposition. High temperature (24°C) and high copper levels caused a coherent increase of a common set of metabolites (mostly glucose, serine, and lysine), indicating that the metabolic impairment due to high temperature is enforced by the presence of copper. Overall, the results demonstrate that, as for human blood plasma, the analysis of haemolymph metabolites represents a promising tool for the diagnosis of pollutant-induced stress syndrome in marine mussels.
Keywords	alanine ; blood proteins ; blood serum ; copper ; glucose ; glutamine ; glutathione ; glycogen ; hemocytes ; humans ; lysine ; metabolites ; metabolomics ; multivariate analysis ; muscles ; mussels ; Mytilus galloprovincialis ; nuclear magnetic resonance spectroscopy ; physiological state ; principal component analysis ; serine ; temperature
Language	English
Dates of publication	2016-05
Size	p. 61-71.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	189285-x
ISSN	0306-4492 ; 0742-8413 ; 1532-0456
ISSN	0306-4492 ; 0742-8413 ; 1532-0456
DOI	10.1016/j.cbpc.2016.02.003
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Impact of Beneficial Microorganisms on Strawberry Growth, Fruit Production, Nutritional Quality, and Volatilome

Valeria Todeschini / Nassima AitLahmidi / Eleonora Mazzucco / Francesco Marsano / Fabio Gosetti / Elisa Robotti / Elisa Bona / Nadia Massa / Laurent Bonneau / Emilio Marengo / Daniel Wipf / Graziella Berta / Guido Lingua

Frontiers in Plant Science, Vol

2018 Volume 9

Abstract: Arbuscular mycorrhizal fungi (AMF) colonize the roots of most terrestrial plant species, improving plant growth, nutrient uptake and biotic/abiotic stress resistance and tolerance. Similarly, plant growth promoting bacteria (PGPB) enhance plant fitness ... ...

Abstract	Arbuscular mycorrhizal fungi (AMF) colonize the roots of most terrestrial plant species, improving plant growth, nutrient uptake and biotic/abiotic stress resistance and tolerance. Similarly, plant growth promoting bacteria (PGPB) enhance plant fitness and production. In this study, three different AMF (Funneliformis mosseae, Septoglomus viscosum, and Rhizophagus irregularis) were used in combination with three different strains of Pseudomonas sp. (19Fv1t, 5Vm1K and Pf4) to inoculate plantlets of Fragaria × ananassa var. Eliana F1. The effects of the different fungus/bacterium combinations were assessed on plant growth parameters, fruit production and quality, including health-promoting compounds. Inoculated and uninoculated plants were maintained in a greenhouse for 4 months and irrigated with a nutrient solution at two different phosphate levels. The number of flowers and fruits were recorded weekly. At harvest, fresh and dry weights of roots and shoots, mycorrhizal colonization and concentration of leaf photosynthetic pigments were measured in each plant. The following fruit parameters were recorded: pH, titratable acids, concentration of organic acids, soluble sugars, ascorbic acids, and anthocyanidins; volatile and elemental composition were also evaluated. Data were statistically analyzed by ANOVA and PCA/PCA-DA. Mycorrhizal colonization was higher in plants inoculated with R. irregularis, followed by F. mosseae and S. viscosum. In general, AMF mostly affected the parameters associated with the vegetative portion of the plant, while PGPB were especially relevant for fruit yield and quality. The plant physiological status was differentially affected by inoculations, resulting in enhanced root and shoot biomass. Inoculation with Pf4 bacterial strain increased flower and fruit production per plant and malic acid content in fruits, while decreased the pH value, regardless of the used fungus. Inoculations affected fruit nutritional quality, increasing sugar and anthocyanin concentrations, and modulated pH, ...
Keywords	PGPB ; AMF ; strawberry ; nutritional quality ; volatile compounds ; sustainable agriculture ; Plant culture ; SB1-1110
Subject code	580 ; 571
Language	English
Publishing date	2018-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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