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  1. AU="Elizete Rizzo"
  2. AU="El Sayegh, Suzanne"
  3. AU="Vaittinen, Tiina"
  4. AU="Khir, Amir S"
  5. AU=Patterson Andrew D
  6. AU="Kim, Joyce Mary"
  7. AU="Saribay, S Adil"
  8. AU="Couderc, M."
  9. AU="Macerlane de Lira Silva"
  10. AU=Neal Michael S
  11. AU="Nakai, Kozo"
  12. AU="Debatin, Jörg F."
  13. AU="Plant, Laura"
  14. AU="Manuel Tisminetzky"
  15. AU="Monaco, Carlo"
  16. AU="Srivastava, Rupesh"
  17. AU="Nathan, Jaimie D"
  18. AU="Schnegelberger, Regina D"
  19. AU=Doshi Paresh
  20. AU="Cecilia Hognon"
  21. AU="Mason, Jeremy K."
  22. AU=Hasumi Hisashi
  23. AU="Swati Sethi"
  24. AU="Martin G. Myers, Jr."
  25. AU="Marcus-Sekura, Carol"
  26. AU="Petagine, Lucy"
  27. AU="Jessa R. Alexander"
  28. AU=Rauner Martina
  29. AU="Richlen, Mindy L"
  30. AU="Merghani, Nada M"
  31. AU=Splitt M P
  32. AU="Zlatanović, Gordana"

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  1. Artikel ; Online: Metapopulation dynamics of the migratory fish Prochilodus lineatus (Characiformes

    Violeta R. Perini / Alessandro L. Paschoalini / Nilo Bazzoli / Elizete Rizzo / Daniel C. Carvalho

    Neotropical Ichthyology, Vol 19, Iss

    Prochilodontidae) in a lotic remnant of the Grande River, Southeastern Brazil

    2021  Band 4

    Abstract: ABSTRACT River impoundments for electricity generation lead to environmental changes which severely affect fish migration and species richness. However, little is known about their effect on the genetic structure and population dynamics downstream from ... ...

    Abstract ABSTRACT River impoundments for electricity generation lead to environmental changes which severely affect fish migration and species richness. However, little is known about their effect on the genetic structure and population dynamics downstream from the reservoir. Here, we analyzed a set of ten microsatellite loci of Prochilodus lineatus, an important South American migratory fish. Specimens (n = 150) were sampled from five sites in a remnant lotic system that includes sections of the Grande, Pardo and Mogi Guaçu rivers, southeastern Brazil. The data showed that all microsatellites were polymorphic with the allele number per locus ranging from 5 to 32, and genetic diversity (H e ) varied from 0.74 to 0.80. Indices of genetic differentiation and Bayesian analysis showed a significant genetic structure and three genetic clusters inhabiting this river system. An asymmetric gene flow suggests source-sink metapopulation dynamics from tributaries (genetic source) to the main river (genetic sink). A genetic cluster that was not detected in the upper Mogi and Pardo rivers tributaries may indicate there is a “trapped gene pool” downstream from the Porto Colômbia dam. Thus, here we provide new insights into the genetic structure and population dynamics of a migratory fish species in a highly dammed river basin.
    Schlagwörter Anthropogenic environmental impact ; Metapopulation dynamics ; Microsatellites ; Migratory species ; Neotropical ; Zoology ; QL1-991
    Thema/Rubrik (Code) 590
    Sprache Englisch
    Erscheinungsdatum 2021-11-01T00:00:00Z
    Verlag Sociedade Brasileira de Ictiologia
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Reproduction of saguiru Steindachnerina elegans (Steindachner, 1874) (Pisces

    LÍVIO GIORGIO L. DE CAMPOS FILHO / LUCAS MARCON / JOSÉ E. DOS SANTOS / KLEBER B. SANTIAGO / ELIZETE RIZZO / NILO BAZZOLI

    Anais da Academia Brasileira de Ciências, Vol 94, Iss

    Curimatidae) in the São Francisco River, downstream from a large reservoir in south-eastern Brazil

    2021  Band 2

    Abstract: Abstract The gametogenesis and reproduction of S. elegans from the São Francisco River at Três Marias, Minas Gerais, were analyzed in this study. Steindachrina elegans is a species abundant in the São Francisco River basin and an important fish in the ... ...

    Abstract Abstract The gametogenesis and reproduction of S. elegans from the São Francisco River at Três Marias, Minas Gerais, were analyzed in this study. Steindachrina elegans is a species abundant in the São Francisco River basin and an important fish in the food chain. The size at first gonadal maturation (indicated by the total length of the shortest spermatid male and spawned female) was 10.5 cm and 15.0 cm, respectively. Reproduction occurred more frequently from September to April. This period had high water temperature values. A high frequency of females was observed at the mature and spawned stages and height gonadosomatic index (GSI). Meanwhile, in males, the reproductive peak was from November to February. The long spawning period and the histological characteristics of the spawned ovaries that contained oocytes at different stages of development along with post-ovulatory and atretic oocytes indicates that the spawning of S. elegans is of the partial type.
    Schlagwörter ovary ; testes ; spawner ; gonadal maturation ; fecundity ; follicles ; Science ; Q
    Thema/Rubrik (Code) 590
    Sprache Englisch
    Erscheinungsdatum 2021-03-01T00:00:00Z
    Verlag Academia Brasileira de Ciências
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Evaluation of the reproductive parameters of female neotropical migratory fish from a lotic and lentic environment of a dammed river

    Luiza Mariz Amador de Souza / Gabriela Gonçalves Theobaldo / Elizete Rizzo / José Enemir dos Santos / Nilo Bazzoli / Alessandro Loureiro Paschoalini

    Tropical Zoology, Vol 34, Iss 1-

    2021  Band 2

    Abstract: The fragmentation of watercourses caused by dams is considered to be one of the main threats to aquatic biodiversity worldwide, especially for ichthyofauna. Several studies have shown that the environmental modifications caused by dammed water bodies can ...

    Abstract The fragmentation of watercourses caused by dams is considered to be one of the main threats to aquatic biodiversity worldwide, especially for ichthyofauna. Several studies have shown that the environmental modifications caused by dammed water bodies can change the various reproductive parameters of freshwater fish. Therefore, the present study aims to comparatively analyze the reproductive potential of female Megaleporinus reinhardti, a migratory species, sampled in the lentic environment of the Três Marias Reservoir and the lotic environment of the São Francisco River. Biometric data were obtained from 79 females and the biological indices were subsequently calculated. Additionally, the microscopic analysis of the gonads was performed, and the follicular atresia index was compared. The results of the study show that fish from the lotic environment presented higher gonadal volume, fecundity, and oocyte diameter, and a lower follicular atresia index when compared to fish from the lentic environment. In summary, the data suggest that environmental changes, i.e. from a lotic to lentic environment, caused by river damming, may negatively affect the reproductive process of migratory fish, such as M. reinhardti, and impair the maintenance of the population of this species in lentic environments
    Schlagwörter Megaleporinus reinhardti ; oogenesis ; fecundity ; neotropical teleosts ; dam impacts ; Zoology ; QL1-991
    Thema/Rubrik (Code) 333
    Sprache Englisch
    Erscheinungsdatum 2021-08-01T00:00:00Z
    Verlag PAGEPress Publications
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Comparative analysis of the reproductive activity of Leporinus piau (Characiformes

    Aline Virtude do Nascimento / Lucas Marcon / José Enemir dos Santos / Kleber Biana Santiago / Elizete Rizzo / Nilo Bazzoli

    Neotropical Ichthyology, Vol 18, Iss

    Anostomidae) in lentic and lotic environments

    2020  Band 4

    Abstract: Abstract In this study, we determined the main reproductive parameters of piau gordura, Leporinus piau, in two sections of the São Francisco River basin. Between May 2015 and April 2016, a total of 573 specimens were captured from a lentic environment ( ... ...

    Abstract Abstract In this study, we determined the main reproductive parameters of piau gordura, Leporinus piau, in two sections of the São Francisco River basin. Between May 2015 and April 2016, a total of 573 specimens were captured from a lentic environment (section 1), the Três Marias Reservoir (TMR), and a lotic environment (section 2), downstream of the TMR at the confluence of the São Francisco River (SFR) with the Abaeté River. Analysis of reproductive activity showed that L. piau from both sections reproduced, but females and males from section 1 exhibited higher total length, body weight, Fulton condition factor, and gonadosomatic index values, as compared to section 2. Sexual dimorphism was evident in the species, with females being larger than males. Moreover, males reached first gonadal maturation at a smaller size than females. The peak maturation/mature stage was observed in November/April for females and males in section 1 and in November/December in section 2, coinciding with high temperatures and precipitation in the region. In both sections of the river, L. piau exhibited the typical characteristics of partial spawning, with a prolonged spawning period, and preferential reproduction in lentic environments.
    Schlagwörter Fecundity ; Follicles ; Gonadal maturation ; Gonadosomatic index ; Zoology ; QL1-991
    Thema/Rubrik (Code) 590
    Sprache Englisch
    Erscheinungsdatum 2020-12-01T00:00:00Z
    Verlag Sociedade Brasileira de Ictiologia
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids

    Ana Paula Barbosa Pinheiro / Rafael Magno Costa Melo / Daniel Fonseca Teixeira / José Luís Olivan Birindelli / Daniel Cardoso Carvalho / Elizete Rizzo

    Scientific Reports, Vol 9, Iss 1, Pp 1-

    2019  Band 12

    Abstract: Abstract Despite its relevance for ecology, evolution and conservation of species, natural hybridization and hybrids biology are still poorly studied in freshwater fish. Here, we tested the hypothesis that sympatric species Astyanax paranae and A. ... ...

    Abstract Abstract Despite its relevance for ecology, evolution and conservation of species, natural hybridization and hybrids biology are still poorly studied in freshwater fish. Here, we tested the hypothesis that sympatric species Astyanax paranae and A. fasciatus are able to interbreed in the natural environment and presented evidence for the first record of hybridization between these species. We analyzed anatomical traits, gametogenesis, reproductive biology, and genetic variations of the COI and S7 genes of both species and putative hybrids. Intermediate morphometric and meristic features were observed in hybrids when compared to A. paranae and A. fasciatus. Overlap in reproductive season was showed for these species, with greater reproductive activity from August to January, but hybrids did not present any sign of gonadal maturation. Oogonia and perinucleolar follicles as well as spermatogonia and primary spermatocytes were found in hybrids, but previtellogenic and vitellogenic follicles, spermatids, and spermatozoa were absent. Moreover, several alterations in gametogenesis were detected, such as interrupted meiosis in both males and females, vacuolated and degenerated germ cells, increased interstitial tissue, and presence of immune cells. Molecular analyses supported the hypothesis of hybridization between A. paranae and A. fasciatus. Overall, our multidisciplinary approach also provides strong evidence that hybrids are infertile.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 590
    Sprache Englisch
    Erscheinungsdatum 2019-03-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Biomarker responses induced by bisphenol A on spermatogenesis in a Neotropical teleost fish are temperature-dependent

    Yves Moreira Ribeiro / André Alberto Weber / Alessandro Loureiro Paschoalini / Davidson Peruci Moreira / Camila Ferreira Sales / Thais Victória Pires de Almeida / Mirra Angelina Neres / Nilo Bazzoli / Elizete Rizzo

    Ecotoxicology and Environmental Safety, Vol 224, Iss , Pp 112670- (2021)

    2021  

    Abstract: Bisphenol A (BPA) is an organic synthetic compound used in the plastic industry with endocrine disrupting activity. Although it is frequently found in surface waters, few studies have investigated its impact on fish gametogenesis, particularly when ... ...

    Abstract Bisphenol A (BPA) is an organic synthetic compound used in the plastic industry with endocrine disrupting activity. Although it is frequently found in surface waters, few studies have investigated its impact on fish gametogenesis, particularly when associated with natural stressors. In this regard, the present study evaluated BPA toxicity on spermatogenesis in the lambari Astyanax bimaculatus under controlled conditions and its interactive effects with water temperature. Adult specimens were exposed in duplicate to 40 μg/L and 400 μg/L BPA at 23 °C and 28 °C for 21 days; the control group did not receive BPA. Testicular samples were collected and analyzed using different cellular and molecular techniques. The results showed a significant reduction in the gonadosomatic index in the BPA-treated groups at both temperatures. A decrease in the testicular levels of 11-ketotestosterone was observed in the 400 μg/L BPA group at 23 °C, 17β-estradiol increased significantly in the treated groups at 28 °C, and vitellogenin showed no difference between the treatments. The morphometric analysis of spermatogenesis revealed a significant increase in the proportion of spermatogonia, spermatocytes, and Sertoli cells in the treated groups, with a higher proportion at 23 °C than at 28 °C. Otherwise, the proportion of spermatozoa was significantly lower in the BPA-treated groups, with a greater reduction at 23 °C. In addition, BPA also stimulated spermatogonial proliferation in the treated groups, but apoptosis was significantly increased in spermatids at 23 °C. Testis-ova, cell degeneration, and chromatin alterations in spermatids and Sertoli cells were observed in the germinal epithelium of the BPA-treated groups. The integrated biomarker response (IBR) index revealed that the analyzed endpoints are suitable for assessing estrogenic contamination. Taken together, our results indicate that the interactive effects of BPA and temperature contribute to the impairment of spermatogenesis in A. bimaculatus with more severe effects ...
    Schlagwörter Reproductive toxicology ; Endocrine disruption ; Cell proliferation ; Apoptosis ; Astyanax bimaculatus ; Sex steroids ; Environmental pollution ; TD172-193.5 ; Environmental sciences ; GE1-350
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2021-11-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel: Stage-specific testicular protein levels of the oestrogen receptors (ERα and ERβ) and Cyp19 and association with oestrogenic contamination in the lambari Astyanax rivularis (Pisces: Characidae)

    Weber, André Alberto / Davidson Peruci Moreira / Rafael Magno Costa Melo / Augusto Bicalho Cruz Vieira / Nilo Bazzoli / Elizete Rizzo

    Environmental science and pollution research international. 2018 Dec., v. 25, no. 34

    2018  

    Abstract: Oestrogens participate in various biological processes such as oogenesis, vitellogenesis and testicular development, but studies regarding the distribution and protein levels of oestrogen receptors (ERα and ERβ) and aromatase (Cyp19) in testis are rarely ...

    Abstract Oestrogens participate in various biological processes such as oogenesis, vitellogenesis and testicular development, but studies regarding the distribution and protein levels of oestrogen receptors (ERα and ERβ) and aromatase (Cyp19) in testis are rarely investigated in fish species. The aim of the present study was to analyse the expression pattern of ERα, ERβ and Cyp19 in testis of Astyanax rivularis and, in addition, to verify if oestrogenic contamination interferes in the expression levels of these proteins. Quarterly, field samplings were carried out during a reproductive cycle in a stream of the Upper Velhas River with a good conservation status (site S1). In the gonadal maturation peak (June), when ripe stage was most abundant, fish collection was made in three streams: S1, reference site, and S2 and S3, sites contaminated by untreated sewage. The results of immunohistochemistry demonstrated labelling of Cyp19 in Leydig cells and acidophilic granulocytes, but spermatogonia, Sertoli cells, spermatids and spermatozoa were also labelled. ERα was more widely distributed than ERβ being found in all developmental germ cell phases. On the other hand, ERβ was found only in spermatogonia and spermatocytes. During testicular maturation, ELISA levels for Cyp19, ERα and ERβ followed the gonadosomatic index (GSI) with significant higher values in the ripe stage. Regarding to endocrine disruption, the males exposed to domestic sewage presented significant higher expression of Cyp19 and ERα when compared to the non-exposed fish. Together, our results demonstrate expression patterns of Cyp19, ERα and ERβ in the testis of A. rivularis. In addition, we indicate ERα and Cyp19 as sensitive biomarkers for monitoring of oestrogenic contamination in freshwater environments.
    Schlagwörter Astyanax ; Leydig cells ; Sertoli cells ; aromatase ; biomarkers ; conservation status ; enzyme-linked immunosorbent assay ; estrogen receptors ; estrogens ; fish ; freshwater ; gonadosomatic index ; granulocytes ; immunohistochemistry ; males ; monitoring ; protein content ; rivers ; sewage ; spermatids ; spermatocytes ; spermatogonia ; spermatozoa ; streams ; testicular development ; vitellogenesis
    Sprache Englisch
    Erscheinungsverlauf 2018-12
    Umfang p. 34403-34413.
    Erscheinungsort Springer Berlin Heidelberg
    Dokumenttyp Artikel
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-018-3392-1
    Datenquelle NAL Katalog (AGRICOLA)

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  8. Artikel: Influence of low temperature on structure and dynamics of spermatogenesis during culture of Oreochromis niloticus

    Melo, Rafael M.C / Elizete Rizzo / Nilo Bazzoli / Ronald K. Luz / Yves M. Ribeiro

    Animal reproduction science. 2016 Sept., v. 172

    2016  

    Abstract: Understanding the influence of different temperature conditions on the spermatogenesis is important for improvement of the fish aquaculture. This study evaluated the influence of low temperature on structural and quantitative dynamics of the ... ...

    Abstract Understanding the influence of different temperature conditions on the spermatogenesis is important for improvement of the fish aquaculture. This study evaluated the influence of low temperature on structural and quantitative dynamics of the spermatogenesis in Oreochromis niloticus. Adult males were cultivated with room temperature water (20.28–22.46°C) and testes were collected for histological, ultrastructural and morphometric analyses. This species has unrestricted lobular testis with cystic spermatogenesis and type I spermiogenesis that results in a single anacrosomal aquasperm. Seminiferous lobules and spermatogenic cells had a radial arrangement toward the spermatic duct. Superior and central portions of testes had a greater lobular area than the inferior portion in all samplings. Spermatogonia (9.3%) were distributed in the inferior portion of testes, spermatocytes (25.3%) and spermatids (34.4%) in the central portion, while spermatozoids (39.4%) and secretory cells (4.6%) in the superior portion. Throughout the study, correlation between water temperature and lobular area characteristics was significant only in the inferior portion of testes (r2=0.95), although the lobular area in the other testicular portions increased when the water temperature increased by 2°C. Correlation between the water temperature and spermatogenic cells was significant for undifferentiated spermatogonia (r2=0.54) and number of spermatids (r2=0.67). It is concluded that low cultivation temperatures may positively influence the generation of primary spermatogonia in the inferior periphery of O. niloticus testes. In addition, males maintain reservoirs of germ cells at low temperatures and the radial zonation of spermatogenesis has an important role in the renewal and production of germ cells.
    Schlagwörter adults ; ambient temperature ; fish culture ; males ; morphometry ; Oreochromis niloticus ; spermatids ; spermatocytes ; spermatogonia ; spermiogenesis ; testes ; water temperature
    Sprache Englisch
    Erscheinungsverlauf 2016-09
    Umfang p. 148-156.
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 429674-6
    ISSN 1873-2232 ; 0378-4320
    ISSN (online) 1873-2232
    ISSN 0378-4320
    DOI 10.1016/j.anireprosci.2016.07.013
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  9. Artikel: Relationship between bcl-2, bax, beclin-1, and cathepsin-D proteins during postovulatory follicular regression in fish ovary

    Morais, Roberto D.V.S / Elizete Rizzo / Hélio B. Santos / Nilo Bazzoli / Ralph G. Thomé

    Theriogenology. 2016 Apr. 01, v. 85, no. 6

    2016  

    Abstract: In fish ovaries, postovulatory follicles (POFs) are key biomarkers of breeding and provide an interesting model for studying the relationship between autophagy and apoptosis. In this study, we investigated the immunohistochemical expression of autophagic ...

    Abstract In fish ovaries, postovulatory follicles (POFs) are key biomarkers of breeding and provide an interesting model for studying the relationship between autophagy and apoptosis. In this study, we investigated the immunohistochemical expression of autophagic and apoptotic proteins to improve the knowledge on the mechanisms regulating ovarian remodeling after spawning. Females from three neotropical fish species kept in captivity were submitted to hormonal induction. After ova stripping, ovarian sections were sampled daily until 5 days postspawning (dps). Similar events of POF regression were detected by histology, terminal transferase-mediated dUTP nick-end labeling (TUNEL), and electron microscopy in the three species: follicular cells hypertrophy, progressive disintegration of the basement membrane, gradual closing of the follicular lumen, theca thickening, and formation of large autophagic vacuoles preceding apoptosis of the follicular cells. Autophagic and apoptotic proteins were assessed by immunohistochemistry. Morphometric analysis of the immunolabeling revealed a more intense reaction for bcl-2 and beclin-1 (BECN1) in POFs at 0 to 1 dps and for bax at 2 to 3 dps (P < 0.001), the later period being the peak of apoptosis of the follicular cells. The immunostaining for cathepsin-D was more elevated until 2 to 3 dps and decreased significantly at 4 to 5 dps, when the POFs were in late stage of regression. Double labeling for BECN1 and caspase-3 indicated a shift in the relationship between autophagy and apoptosis at 2 to 3 dps, a critical period in determining the fate of follicular cells in POFs. Together, these results indicate that the bcl-2 family, BECN1, and cathepsin-D can be involved in the regulation of ovarian remodeling in teleost fish.
    Schlagwörter apoptosis ; autophagy ; basement membrane ; biomarkers ; breeding ; captive animals ; caspase-3 ; cathepsin D ; electron microscopy ; females ; fish ; hypertrophy ; immunohistochemistry ; models ; morphometry ; ova ; proteins ; spawning ; tropics ; vacuoles
    Sprache Englisch
    Erscheinungsverlauf 2016-0401
    Umfang p. 1118-1131.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel
    ZDB-ID 189232-0
    ISSN 1879-3231 ; 0093-691X
    ISSN (online) 1879-3231
    ISSN 0093-691X
    DOI 10.1016/j.theriogenology.2015.11.024
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  10. Artikel: Reproductive biomarkers responses induced by xenoestrogens in the characid fish Astyanax fasciatus inhabiting a South American reservoir: An integrated field and laboratory approach

    Prado, Paula S / Ana Paula B. Pinheiro / Elizete Rizzo / Nilo Bazzoli

    Environmental Research. 2014 May, v. 131

    2014  

    Abstract: Field studies evaluating the effects of endocrine disruption chemicals (EDCs) on the fish reproduction are scarce worldwide. The goal of this study was to assess hepatic levels of vitellogenin (Vtg), zona radiata proteins (Zrp) and insulin-like growth ... ...

    Abstract Field studies evaluating the effects of endocrine disruption chemicals (EDCs) on the fish reproduction are scarce worldwide. The goal of this study was to assess hepatic levels of vitellogenin (Vtg), zona radiata proteins (Zrp) and insulin-like growth factors (IGF-I and IGF-II), and relating them to reproductive endpoints in a wild fish population habiting a reservoir that receive domestic sewage, agricultural and industrial residues. Adult fish Astyanax fasciatus were sampled during the reproductive season in five sites from the Furnas Reservoir, Grande River, and Paraguay–Paraná basin. As a control to field data, fish were experimentally exposed via dietary intake, to oestradiol benzoate (OB) for 7 days. Fish from site with little anthropogenic interference showed hepatic levels of Vtg, Zrp and IGF-I and IGF-II similar to those from the non-treated experimental group. In sites located immediately downstream from the municipal wastewater discharges, the water total oestrogen was >120ng/l, and male fish displayed increased Vtg and Zrp and decreased IGF-I levels similar to OB treated fish. In females, levels of Vtg, Zrp, IGF-I and IGF-II suggest an impairment of final oocyte maturation and spawning, as also detected by frequency of over-ripening, follicular atresia and fecundity. At the sites that receive agricultural and industrial residues, the water total oestrogen was <50ng/l and females showed decreased Zrp and increased IGF-II levels associated to reduced diameter of vitellogenic follicles, indicating an inhibition of oocyte growth. Overall, the current study reports oestrogenic contamination impairing the reproduction of a wild fish from a hydroeletric reservoir and, the data contribute to improving the current knowledge on relationship between hepatic Vtg, Zrp and IGF-I and IGF-II, and reproductive endpoints in a teleost fish. In addition, our data point out novel reproductive biomarkers (IGF-I, IGF-II and over-ripening) to assessing xenoestrogenic contamination in freshwater ecosystems.
    Schlagwörter adults ; Astyanax fasciatus ; basins ; biomarkers ; breeding season ; estradiol ; fecundity ; females ; follicular atresia ; food intake ; freshwater ecosystems ; insulin-like growth factor I ; insulin-like growth factor II ; males ; municipal wastewater ; oocytes ; rivers ; sewage ; spawning ; vitellogenin ; wild fish ; xenoestrogens
    Sprache Englisch
    Erscheinungsverlauf 2014-05
    Umfang p. 165-173.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel
    ZDB-ID 205699-9
    ISSN 1096-0953 ; 0013-9351
    ISSN (online) 1096-0953
    ISSN 0013-9351
    DOI 10.1016/j.envres.2014.03.002
    Datenquelle NAL Katalog (AGRICOLA)

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