Article ; Online: Selective Schiff base formation via gas-phase ion/ion reactions to enable differentiation of isobaric lipids in imaging mass spectrometry.
Analytical and bioanalytical chemistry
2023 Volume 415, Issue 18, Page(s) 4319–4331
Abstract: The separation and identification of lipids in complex mixtures are critical to deciphering their cellular functions. Failure to resolve isobaric compounds (e.g., via high mass resolution or tandem mass spectrometry) can result in incorrect ... ...
| Abstract | The separation and identification of lipids in complex mixtures are critical to deciphering their cellular functions. Failure to resolve isobaric compounds (e.g., via high mass resolution or tandem mass spectrometry) can result in incorrect identifications in mass spectrometry experiments. In imaging mass spectrometry, unresolved peaks can also result in composite images of multiple compounds, giving inaccurate depictions of molecular distributions. Gas-phase ion/ion reactions can be used to selectively react with specific chemical functional groups on a target analyte, thereby extracting it from a complex mixture and shifting its m/z value to an unobstructed region of the mass range. Herein, we use selective Schiff base formation via a novel charge inversion ion/ion reaction to purify phosphatidylserines from other isobaric (i.e., same nominal mass) lipids and reveal their singular distributions in imaging mass spectrometry. The selective Schiff base formation between singly deprotonated phosphatidylserine (PS) lipid anions and doubly charged N,N,N',N'-tetramethyl-N,N'-bis(6-oxohexyl)hexane-1,6-diaminium (TMODA) cations is performed using a modified commercial dual source hybrid Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. This process is demonstrated using the isobaric lipids [PS 40:6 - H] |
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| MeSH term(s) | Schiff Bases/chemistry ; Anions ; Cations ; Tandem Mass Spectrometry ; Lipids |
| Chemical Substances | Schiff Bases ; Anions ; Cations ; Lipids |
| Language | English |
| Publishing date | 2023-01-11 |
| Publishing country | Germany |
| Document type | Journal Article |
| ZDB-ID | 201093-8 |
| ISSN | 1618-2650 ; 0016-1152 ; 0372-7920 |
| ISSN (online) | 1618-2650 |
| ISSN | 0016-1152 ; 0372-7920 |
| DOI | 10.1007/s00216-023-04523-y |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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