LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article ; Online: An in-solution snapshot of SARS-COV-2 main protease maturation process and inhibition.

    Noske, Gabriela Dias / Song, Yun / Fernandes, Rafaela Sachetto / Chalk, Rod / Elmassoudi, Haitem / Koekemoer, Lizbé / Owen, C David / El-Baba, Tarick J / Robinson, Carol V / Oliva, Glaucius / Godoy, Andre Schutzer

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 1545

    Abstract: The main protease from SARS-CoV-2 ( ... ...

    Abstract The main protease from SARS-CoV-2 (M
    MeSH term(s) Antiviral Agents ; Protease Inhibitors/pharmacology ; SARS-CoV-2/enzymology ; Coronavirus 3C Proteases/chemistry
    Chemical Substances 3C-like proteinase, SARS-CoV-2 (EC 3.4.22.-) ; Antiviral Agents ; Protease Inhibitors ; Coronavirus 3C Proteases (EC 3.4.22.28)
    Language English
    Publishing date 2023-03-20
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-37035-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: A novel, essential trans-splicing protein connects the nematode SL1 snRNP to the CBC-ARS2 complex.

    Fasimoye, Rotimi Yemi / Spencer, Rosie Elizabeth Barker / Soto-Martin, Eva / Eijlers, Peter / Elmassoudi, Haitem / Brivio, Sarah / Mangana, Carolina / Sabele, Viktorija / Rechtorikova, Radoslava / Wenzel, Marius / Connolly, Bernadette / Pettitt, Jonathan / Müller, Berndt

    Nucleic acids research

    2022  Volume 50, Issue 13, Page(s) 7591–7607

    Abstract: Spliced leader trans-splicing is essential for gene expression in many eukaryotes. To elucidate the molecular mechanism of this process, we characterise the molecules associated with the Caenorhabditis elegans major spliced leader snRNP (SL1 snRNP), ... ...

    Abstract Spliced leader trans-splicing is essential for gene expression in many eukaryotes. To elucidate the molecular mechanism of this process, we characterise the molecules associated with the Caenorhabditis elegans major spliced leader snRNP (SL1 snRNP), which donates the spliced leader that replaces the 5' untranslated region of most pre-mRNAs. Using a GFP-tagged version of the SL1 snRNP protein SNA-1 created by CRISPR-mediated genome engineering, we immunoprecipitate and identify RNAs and protein components by RIP-Seq and mass spectrometry. This reveals the composition of the SL1 snRNP and identifies associations with spliceosome components PRP-8 and PRP-19. Significantly, we identify a novel, nematode-specific protein required for SL1 trans-splicing, which we designate SNA-3. SNA-3 is an essential, nuclear protein with three NADAR domains whose function is unknown. Mutation of key residues in NADAR domains inactivates the protein, indicating that domain function is required for activity. SNA-3 interacts with the CBC-ARS2 complex and other factors involved in RNA metabolism, including SUT-1 protein, through RNA or protein-mediated contacts revealed by yeast two-hybrid assays, localisation studies and immunoprecipitations. Our data are compatible with a role for SNA-3 in coordinating trans-splicing with target pre-mRNA transcription or in the processing of the Y-branch product of the trans-splicing reaction.
    MeSH term(s) Animals ; 5' Untranslated Regions ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/metabolism ; Nuclear Proteins/metabolism ; Ribonucleoproteins, Small Nuclear/genetics ; RNA Splicing ; RNA, Helminth/genetics ; RNA, Spliced Leader/genetics ; Trans-Splicing
    Chemical Substances 5' Untranslated Regions ; Caenorhabditis elegans Proteins ; Nuclear Proteins ; Ribonucleoproteins, Small Nuclear ; RNA, Helminth ; RNA, Spliced Leader ; sna-3 protein, C elegans
    Language English
    Publishing date 2022-06-27
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkac534
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top