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  1. AU="Elrod, Virginia A"
  2. AU=Alpaydin Aylin O AU=Alpaydin Aylin O
  3. AU=Amin Kritika Premnath
  4. AU="Subramanian, Prem S"
  5. AU="S.Wang, "
  6. AU="Kotrotsiou, Evagelia"
  7. AU="Belnap, Ethan"
  8. AU="Luo, Jianming"
  9. AU=Hsu Pin-Jui
  10. AU=Debaud Charlotte
  11. AU="García-Rubia, Alfonso"
  12. AU=Belot Alexandre
  13. AU="Jorge Ospina Duque"
  14. AU="Chalot, Michel"
  15. AU="Gunsolley, J N"
  16. AU="Tuè, Giovanni"
  17. AU="de Laat, Bart"
  18. AU=Drory Omri
  19. AU="Pothlichet, Julien"
  20. AU="Douwes, Rianne M"
  21. AU="Jarzebowski, Mary"
  22. AU="Shetty, Sunil"
  23. AU="Ricardo Ryoshim Kunyioshi"
  24. AU=Santos Maria Cristina Leme Godoy Dos AU=Santos Maria Cristina Leme Godoy Dos

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  1. Artikel ; Online: Deep-Sea DuraFET: A Pressure Tolerant pH Sensor Designed for Global Sensor Networks.

    Johnson, Kenneth S / Jannasch, Hans W / Coletti, Luke J / Elrod, Virginia A / Martz, Todd R / Takeshita, Yuichiro / Carlson, Robert J / Connery, James G

    Analytical chemistry

    2016  Band 88, Heft 6, Seite(n) 3249–3256

    Abstract: Increasing atmospheric carbon dioxide is driving a long-term decrease in ocean pH which is superimposed on daily to seasonal variability. These changes impact ecosystem processes, and they serve as a record of ecosystem metabolism. However, the temporal ... ...

    Abstract Increasing atmospheric carbon dioxide is driving a long-term decrease in ocean pH which is superimposed on daily to seasonal variability. These changes impact ecosystem processes, and they serve as a record of ecosystem metabolism. However, the temporal variability in pH is observed at only a few locations in the ocean because a ship is required to support pH observations of sufficient precision and accuracy. This paper describes a pressure tolerant Ion Sensitive Field Effect Transistor pH sensor that is based on the Honeywell Durafet ISFET die. When combined with a AgCl pseudoreference sensor that is immersed directly in seawater, the system is capable of operating for years at a time on platforms that cycle from depths of several km to the surface. The paper also describes the calibration scheme developed to allow calibrated pH measurements to be derived from the activity of HCl reported by the sensor system over the range of ocean pressure and temperature. Deployments on vertical profiling platforms enable self-calibration in deep waters where pH values are stable. Measurements with the sensor indicate that it is capable of reporting pH with an accuracy of 0.01 or better on the total proton scale and a precision over multiyear periods of 0.005. This system enables a global ocean observing system for ocean pH.
    Sprache Englisch
    Erscheinungsdatum 2016-03-15
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.5b04653
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  2. Artikel: Deep-Sea DuraFET: A Pressure Tolerant pH Sensor Designed for Global Sensor Networks

    Johnson, Kenneth S / Carlson Robert J / Coletti Luke J / Connery James G / Elrod Virginia A / Jannasch Hans W / Martz Todd R / Takeshita Yuichiro

    Analytical chemistry. 2016 Mar. 15, v. 88, no. 6

    2016  

    Abstract: Increasing atmospheric carbon dioxide is driving a long-term decrease in ocean pH which is superimposed on daily to seasonal variability. These changes impact ecosystem processes, and they serve as a record of ecosystem metabolism. However, the temporal ... ...

    Abstract Increasing atmospheric carbon dioxide is driving a long-term decrease in ocean pH which is superimposed on daily to seasonal variability. These changes impact ecosystem processes, and they serve as a record of ecosystem metabolism. However, the temporal variability in pH is observed at only a few locations in the ocean because a ship is required to support pH observations of sufficient precision and accuracy. This paper describes a pressure tolerant Ion Sensitive Field Effect Transistor pH sensor that is based on the Honeywell Durafet ISFET die. When combined with a AgCl pseudoreference sensor that is immersed directly in seawater, the system is capable of operating for years at a time on platforms that cycle from depths of several km to the surface. The paper also describes the calibration scheme developed to allow calibrated pH measurements to be derived from the activity of HCl reported by the sensor system over the range of ocean pressure and temperature. Deployments on vertical profiling platforms enable self-calibration in deep waters where pH values are stable. Measurements with the sensor indicate that it is capable of reporting pH with an accuracy of 0.01 or better on the total proton scale and a precision over multiyear periods of 0.005. This system enables a global ocean observing system for ocean pH.
    Schlagwörter carbon dioxide ; ecosystems ; environmental impact ; hydrochloric acid ; metabolism ; oceans ; pH ; seasonal variation ; seawater ; silver chloride ; temperature
    Sprache Englisch
    Erscheinungsverlauf 2016-0315
    Umfang p. 3249-3256.
    Erscheinungsort American Chemical Society
    Dokumenttyp Artikel
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021%2Facs.analchem.5b04653
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  3. Artikel ; Online: Abundance and Biogeography of Picoprasinophyte Ecotypes and Other Phytoplankton in the Eastern North Pacific Ocean.

    Simmons, Melinda P / Sudek, Sebastian / Monier, Adam / Limardo, Alexander J / Jimenez, Valeria / Perle, Christopher R / Elrod, Virginia A / Pennington, J Timothy / Worden, Alexandra Z

    Applied and environmental microbiology

    2016  Band 82, Heft 6, Seite(n) 1693–1705

    Abstract: Eukaryotic algae within the picoplankton size class (≤2 μm in diameter) are important marine primary producers, but their spatial and ecological distributions are not well characterized. Here, we studied three picoeukaryotic prasinophyte genera and their ...

    Abstract Eukaryotic algae within the picoplankton size class (≤2 μm in diameter) are important marine primary producers, but their spatial and ecological distributions are not well characterized. Here, we studied three picoeukaryotic prasinophyte genera and their cyanobacterial counterparts, Prochlorococcus and Synechococcus, during two cruises along a North Pacific transect characterized by different ecological regimes. Picoeukaryotes and Synechococcus reached maximum abundances of 1.44 × 10(5) and 3.37 × 10(5) cells · ml(-1), respectively, in mesotrophic waters, while Prochlorococcus reached 1.95 × 10(5) cells · ml(-1) in the oligotrophic ocean. Of the picoeukaryotes, Bathycoccus was present at all stations in both cruises, reaching 21,368 ± 327 18S rRNA gene copies · ml(-1). Micromonas and Ostreococcus clade OI were detected only in mesotrophic and coastal waters and Ostreococcus clade OII only in the oligotrophic ocean. To resolve proposed Bathycoccus ecotypes, we established genetic distances for 1,104 marker genes using targeted metagenomes and the Bathycoccus prasinos genome. The analysis was anchored in comparative genome analysis of three Ostreococcus species for which physiological and environmental data are available to facilitate data interpretation. We established that two Bathycoccus ecotypes exist, named here BI (represented by coastal isolate Bathycoccus prasinos) and BII. These share 82% ± 6% nucleotide identity across homologs, while the Ostreococcus spp. share 75% ± 8%. We developed and applied an analysis of ecomarkers to metatranscriptomes sequenced here and published -omics data from the same region. The results indicated that the Bathycoccus ecotypes cooccur more often than Ostreococcus clades OI and OII do. Exploratory analyses of relative transcript abundances suggest that Bathycoccus NRT2.1 and AMT2.2 are high-affinity NO3 (-) and low-affinity NH4 (+) transporters, respectively, with close homologs in multiple picoprasinophytes. Additionally, in the open ocean, where dissolved iron concentrations were low (0.08 nM), there appeared to be a shift to the use of nickel superoxide dismutases (SODs) from Mn/Fe/Cu SODs closer inshore. Our study documents the distribution of picophytoplankton along a North Pacific ecological gradient and offers new concepts and techniques for investigating their biogeography.
    Mesh-Begriff(e) Chlorophyta/classification ; Chlorophyta/genetics ; DNA, Plant/chemistry ; DNA, Plant/genetics ; DNA, Ribosomal/chemistry ; DNA, Ribosomal/genetics ; Ecotype ; Metagenomics ; Pacific Ocean ; Phylogeography ; Phytoplankton/classification ; Phytoplankton/genetics ; Phytoplankton/isolation & purification ; RNA, Ribosomal, 18S/genetics ; Seawater/microbiology ; Sequence Analysis, DNA
    Chemische Substanzen DNA, Plant ; DNA, Ribosomal ; RNA, Ribosomal, 18S
    Sprache Englisch
    Erscheinungsdatum 2016-01-04
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.02730-15
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

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  4. Artikel ; Online: Specialized proteomic responses and an ancient photoprotection mechanism sustain marine green algal growth during phosphate limitation.

    Guo, Jian / Wilken, Susanne / Jimenez, Valeria / Choi, Chang Jae / Ansong, Charles / Dannebaum, Richard / Sudek, Lisa / Milner, David S / Bachy, Charles / Reistetter, Emily Nahas / Elrod, Virginia A / Klimov, Denis / Purvine, Samuel O / Wei, Chia-Lin / Kunde-Ramamoorthy, Govindarajan / Richards, Thomas A / Goodenough, Ursula / Smith, Richard D / Callister, Stephen J /
    Worden, Alexandra Z

    Nature microbiology

    2018  Band 3, Heft 7, Seite(n) 781–790

    Abstract: Marine algae perform approximately half of global carbon fixation, but their growth is often limited by the availability of phosphate or other ... ...

    Abstract Marine algae perform approximately half of global carbon fixation, but their growth is often limited by the availability of phosphate or other nutrients
    Mesh-Begriff(e) Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bioreactors/parasitology ; Chlorophyta/classification ; Chlorophyta/growth & development ; Chlorophyta/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Phosphates/metabolism ; Photosynthesis ; Phylogeny ; Phytoplankton ; Proteomics/methods
    Chemische Substanzen Bacterial Proteins ; Phosphates
    Sprache Englisch
    Erscheinungsdatum 2018-06-25
    Erscheinungsland England
    Dokumenttyp Letter ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 2058-5276
    ISSN (online) 2058-5276
    DOI 10.1038/s41564-018-0178-7
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Abundance and biogeography of picoprasinophyte ecotypes and other phytoplankton in the eastern North Pacific Ocean

    Simmons, Melinda P. / Sudek, Sebastian / Monier, Adam / Limardo, Alexander J. / Jimenez, Valeria / Perle, Christopher R. / Elrod, Virginia A. / Pennington, J. Timothy / Worden, Alexandra Z.

    2016  

    Abstract: Eukaryotic algae within the picoplankton size class (< 2 μmin diameter) are important marine primary producers, but their spatial and ecological distributions are not well characterized. Here, we studied three picoeukaryotic prasinophyte genera and their ...

    Abstract Eukaryotic algae within the picoplankton size class (< 2 μmin diameter) are important marine primary producers, but their spatial and ecological distributions are not well characterized. Here, we studied three picoeukaryotic prasinophyte genera and their cyanobacterial counterparts, Prochlorococcus and Synechococcus, during two cruises along a North Pacific transect characterized by different ecological regimes. Picoeukaryotes and Synechococcus reached maximum abundances of 1.44 × 105 and 3.37 × 105 cells · ml-1, respectively, in mesotrophic waters, while Prochlorococcus reached 1.95 × 105 cells · ml-1 in the oligotrophic ocean. Of the picoeukaryotes, Bathycoccus was present at all stations in both cruises, reaching 21,368±327 18S rRNA gene copies · ml-1. Micromonas and Ostreococcus clade OI were detected only in mesotrophic and coastal waters and Ostreococcus clade OII only in the oligotrophic ocean. To resolve proposed Bathycoccus ecotypes, we established genetic distances for 1,104 marker genes using targeted metagenomes and the Bathycoccus prasinos genome. The analysis was anchored in comparative genome analysis of three Ostreococcus species for which physiological and environmental data are available to facilitate data interpretation. We established that two Bathycoccus ecotypes exist, named here BI (represented by coastal isolate Bathycoccus prasinos) and BII. These share 82±6 nucleotide identity across homologs, while the Ostreococcus spp. share 75±8. We developed and applied an analysis of ecomarkers to metatranscriptomes sequenced here and published -omics data from the same region. The results indicated that the Bathycoccus ecotypes cooccur more often than Ostreococcus clades OI and OII do. Exploratory analyses of relative transcript abundances suggest that Bathycoccus NRT2.1 and AMT2.2 are high-affinity NO3 - and low-affinity NH4 + transporters, respectively, with close homologs in multiple picoprasinophytes. Additionally, in the open ocean, where dissolved iron concentrations were low (0.08 nM), ...
    Thema/Rubrik (Code) 551
    Sprache Englisch
    Verlag American Society for Microbiology
    Erscheinungsland de
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Southern Ocean iron enrichment experiment: carbon cycling in high- and low-Si waters.

    Coale, Kenneth H / Johnson, Kenneth S / Chavez, Francisco P / Buesseler, Ken O / Barber, Richard T / Brzezinski, Mark A / Cochlan, William P / Millero, Frank J / Falkowski, Paul G / Bauer, James E / Wanninkhof, Rik H / Kudela, Raphael M / Altabet, Mark A / Hales, Burke E / Takahashi, Taro / Landry, Michael R / Bidigare, Robert R / Wang, Xiujun / Chase, Zanna /
    Strutton, Pete G / Friederich, Gernot E / Gorbunov, Maxim Y / Lance, Veronica P / Hilting, Anna K / Hiscock, Michael R / Demarest, Mark / Hiscock, William T / Sullivan, Kevin F / Tanner, Sara J / Gordon, R Mike / Hunter, Craig N / Elrod, Virginia A / Fitzwater, Steve E / Jones, Janice L / Tozzi, Sasha / Koblizek, Michal / Roberts, Alice E / Herndon, Julian / Brewster, Jodi / Ladizinsky, Nicolas / Smith, Geoffrey / Cooper, David / Timothy, David / Brown, Susan L / Selph, Karen E / Sheridan, Cecelia C / Twining, Benjamin S / Johnson, Zackary I

    Science (New York, N.Y.)

    2004  Band 304, Heft 5669, Seite(n) 408–414

    Abstract: The availability of iron is known to exert a controlling influence on biological productivity in surface waters over large areas of the ocean and may have been an important factor in the variation of the concentration of atmospheric carbon dioxide over ... ...

    Abstract The availability of iron is known to exert a controlling influence on biological productivity in surface waters over large areas of the ocean and may have been an important factor in the variation of the concentration of atmospheric carbon dioxide over glacial cycles. The effect of iron in the Southern Ocean is particularly important because of its large area and abundant nitrate, yet iron-enhanced growth of phytoplankton may be differentially expressed between waters with high silicic acid in the south and low silicic acid in the north, where diatom growth may be limited by both silicic acid and iron. Two mesoscale experiments, designed to investigate the effects of iron enrichment in regions with high and low concentrations of silicic acid, were performed in the Southern Ocean. These experiments demonstrate iron's pivotal role in controlling carbon uptake and regulating atmospheric partial pressure of carbon dioxide.
    Mesh-Begriff(e) Atmosphere ; Biomass ; Carbon/analysis ; Carbon/metabolism ; Carbon Dioxide/analysis ; Carbon Dioxide/metabolism ; Chlorophyll/analysis ; Diatoms/growth & development ; Diatoms/metabolism ; Ecosystem ; Iron/analysis ; Iron/metabolism ; Nitrates/analysis ; Nitrates/metabolism ; Nitrogen/analysis ; Nitrogen/metabolism ; Oceans and Seas ; Photosynthesis ; Phytoplankton/growth & development ; Phytoplankton/metabolism ; Seawater/chemistry ; Silicic Acid/analysis ; Silicic Acid/metabolism
    Chemische Substanzen Nitrates ; Silicic Acid (1343-98-2) ; Chlorophyll (1406-65-1) ; Carbon Dioxide (142M471B3J) ; Carbon (7440-44-0) ; Iron (E1UOL152H7) ; Nitrogen (N762921K75) ; chlorophyll a (YF5Q9EJC8Y)
    Sprache Englisch
    Erscheinungsdatum 2004-04-16
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    DOI 10.1126/science.1089778
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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