LIVIVO - Search results -

Search results

Result 1 - 10 of total 253

Search options

Article ; Online: Exploring Mechanisms of Biotic Chlorinated Alkane Reduction: Evidence of Nucleophilic Substitution (S

Heckel, Benjamin / Elsner, Martin

Environmental science & technology

2022 Volume 56, Issue 10, Page(s) 6325–6336

Abstract: Chlorinated alkanes are notorious groundwater contaminants. Their natural reductive dechlorination by microorganisms involves reductive dehalogenases (RDases) containing cobamide as a cofactor. However, underlying mechanisms of reductive dehalogenation ... ...

Abstract	Chlorinated alkanes are notorious groundwater contaminants. Their natural reductive dechlorination by microorganisms involves reductive dehalogenases (RDases) containing cobamide as a cofactor. However, underlying mechanisms of reductive dehalogenation have remained uncertain. Here, observed products, radical trap experiments, UV-vis, and mass spectra demonstrate that (i) reduction by cobalamin (vitamin B
MeSH term(s)	Alkanes ; Biodegradation, Environmental ; Carbon Isotopes ; Groundwater ; Vitamin B 12/analysis ; Vitamins/analysis
Chemical Substances	Alkanes ; Carbon Isotopes ; Vitamins ; Vitamin B 12 (P6YC3EG204)
Language	English
Publishing date	2022-04-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.1c06066
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Book ; Thesis: Renale und hämodynamische Effekte durch Hochdosis-Kontrastmittel-Applikationen im Rahmen der Koronarangiographie

Elsner, Martin

eine prospektive, randomisierte Doppelblind-Studie

2001

Author's details	vorgelegt von Martin Elsner
Language	German
Size	95 Bl. : zahlr. Ill.
Publishing country	Germany
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Essen, Univ., Diss., 2002
HBZ-ID	HT013418810
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

Shelf mark	Loan status	Location
2002 E 3946	order for loan	Magazin

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Exploring Mechanisms of Biotic Chlorinated Alkane Reduction: Evidence of Nucleophilic Substitution (SN2) with Vitamin B₁₂

Heckel, Benjamin / Elsner, Martin

Environmental science & technology. 2022 Apr. 25, v. 56, no. 10

2022

Abstract	Chlorinated alkanes are notorious groundwater contaminants. Their natural reductive dechlorination by microorganisms involves reductive dehalogenases (RDases) containing cobamide as a cofactor. However, underlying mechanisms of reductive dehalogenation have remained uncertain. Here, observed products, radical trap experiments, UV–vis, and mass spectra demonstrate that (i) reduction by cobalamin (vitamin B₁₂) involved chloroalkyl–cobalamin complexes (ii) whose formation involved a second-order nucleophilic substitution (SN2). Dual element isotope analysis subsequently linked insights from our model system to microbial reductive dehalogenation. Identical observed isotope effects in reduction of trichloromethane by Dehalobacter CF and cobalamin (Dehalobacter CF, εC = −27.9 ± 1.7‰; εCₗ = −4.2 ± 0.‰; λ = 6.6 ± 0.1; cobalamin, εC = −26.0 ± 0.9‰; εCₗ = −4.0 ± 0.2‰; λ = 6.5 ± 0.2) indicated the same underlying mechanism, as did identical isotope effects in the reduction of 1,2-dichloroethane by Dehalococcoides and cobalamin (Dehalococcoides, εC = −33.0 ± 0.4‰; εCₗ = −5.1 ± 0.1‰; λ = 6.5 ± 0.2; cobalamin, εC = −32.8 ± 1.7‰; εCₗ = −5.1 ± 0.2‰; λ = 6.4 ± 0.2). In contrast, a different, non-SN2 reaction was evidenced by different isotope effects in reaction of 1,2-dichloroethane with Dehalogenimonas (εC = −23.0 ± 2.0‰; εCₗ = −12.0 ± 0.8‰; λ = 1.9 ± 0.02) illustrating a diversity of biochemical reaction mechanisms manifested even within the same class of enzymes (RDases). This study resolves open questions in our understanding of bacterial reductive dehalogenation and, thereby, provides important information on the biochemistry of bioremediation.
Keywords	Dehalobacter ; Dehalococcoides ; Lewis bases ; alkanes ; bioremediation ; dechlorination ; ethylene dichloride ; groundwater contamination ; isotopes ; technology ; vitamin B12
Language	English
Dates of publication	2022-0425
Size	p. 6325-6336.
Publishing place	American Chemical Society
Document type	Article
ISSN	1520-5851
DOI	10.1021/acs.est.1c06066
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Microbial hydrolysis of atrazine in contaminated groundwater.

Arar, Mohammad / Bakkour, Rani / Elsner, Martin / Bernstein, Anat

Chemosphere

2023 Volume 322, Page(s) 138226

Abstract: Degradation of the widespread herbicide atrazine has been intensively studied in soils, while its degradation in groundwater has received less attention. This work studied atrazine degradation in contaminated groundwater adjacent to its production plant. ...

Abstract	Degradation of the widespread herbicide atrazine has been intensively studied in soils, while its degradation in groundwater has received less attention. This work studied atrazine degradation in contaminated groundwater adjacent to its production plant. The degradation potential was first explored in groundwater enrichment cultures. A broad potential for microbial atrazine degradation was observed when atrazine served as the sole nitrogen source, even when incubated with nitrate. Hydroxyatrazine was formed by the cultures, while desethylatrazine and desisopropylatrazine were not detected. Both the atzA and the trzN genes were identified by quantitative PCR analysis, with a clear dominance of atzA. Carbon isotope enrichments throughout the degradation process varied between the different cultures, with ε values ranging from -0.6 to -5.5‰. This implies corresponding uncertainties when using compound-specific isotope analysis to estimate degradation extents. In the field samples, in-situ degradation was reflected by a high percentage of metabolites, with hydroxyatrazine accounting for >95% of the metabolites in most wells. Both atzA and trzN were detected in the groundwater at quantities of ≈10
MeSH term(s)	Atrazine/analysis ; Hydrolysis ; Herbicides/analysis ; Carbon Isotopes/analysis ; Groundwater ; Biodegradation, Environmental
Chemical Substances	Atrazine (QJA9M5H4IM) ; Herbicides ; Carbon Isotopes
Language	English
Publishing date	2023-02-22
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2023.138226
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2540: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Avoiding Interferences in Advance: Cyclodextrin Polymers to Enhance Selectivity in Extraction of Organic Micropollutants for Carbon Isotope Analysis.

Glöckler, David / Wabnitz, Christopher / Elsner, Martin / Bakkour, Rani

Analytical chemistry

2023 Volume 95, Issue 20, Page(s) 7839–7848

Abstract: Compound-specific isotope analysis (CSIA) of organic water contaminants can provide important information about their sources and fate in the environment. Analyte enrichment from water remains nonetheless a critical yet inevitable step before measurement. ...

Abstract	Compound-specific isotope analysis (CSIA) of organic water contaminants can provide important information about their sources and fate in the environment. Analyte enrichment from water remains nonetheless a critical yet inevitable step before measurement. Commercially available solid-phase extraction (SPE) sorbents are inherently nonselective leading to co-extraction of concurrent dissolved organic matter (DOM) and in turn to analytical interferences, especially for low-occurring contaminants. Here, we (i) increased extraction selectivity by synthesizing cyclodextrin polymers (α-, β-, γ-CDP) as SPE sorbents, (ii) assessed their applicability to carbon isotope analysis for a selection of pesticides, and (iii) compared them with commonly used commercial sorbents. Extraction with β-CDP significantly reduced backgrounds in gas chromatography-isotope ratio mass spectrometry (GC-IRMS) and enhanced sensitivity by a factor of 7.5, which was further confirmed by lower carbon-normalized C
Language	English
Publishing date	2023-05-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c05465
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4033: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Avoiding Interferences in Advance: Cyclodextrin Polymers to Enhance Selectivity in Extraction of Organic Micropollutants for Carbon Isotope Analysis

Glöckler, David / Wabnitz, Christopher / Elsner, Martin / Bakkour, Rani

Analytical Chemistry. 2023 May 11, v. 95, no. 20 p.7839-7848

2023

Abstract	Compound-specific isotope analysis (CSIA) of organic water contaminants can provide important information about their sources and fate in the environment. Analyte enrichment from water remains nonetheless a critical yet inevitable step before measurement. Commercially available solid-phase extraction (SPE) sorbents are inherently nonselective leading to co-extraction of concurrent dissolved organic matter (DOM) and in turn to analytical interferences, especially for low-occurring contaminants. Here, we (i) increased extraction selectivity by synthesizing cyclodextrin polymers (α-, β-, γ-CDP) as SPE sorbents, (ii) assessed their applicability to carbon isotope analysis for a selection of pesticides, and (iii) compared them with commonly used commercial sorbents. Extraction with β-CDP significantly reduced backgrounds in gas chromatography-isotope ratio mass spectrometry (GC-IRMS) and enhanced sensitivity by a factor of 7.5, which was further confirmed by lower carbon-normalized CDOM/Cₐₙₐₗyₜₑ ratios in corresponding extracts as derived from dissolved organic carbon (DOC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Gibbs free energies of adsorption demonstrated weak competition between DOM and analyte on the three CDPs. No isotopic fractionation (Δδ¹³C within ± 0.3‰) was observed for the investigated pesticides after using β-CDP as an SPE sorbent covering a range of concentrations (5–500 μg L–¹), flow velocities (5–40 cm min–¹), and sorbent regeneration (up to six times). The present study highlights the benefit of selecting innovative extraction sorbents to avoid interferences in advance. This strategy in combination with existing cleanup approaches offers new prospects for CSIA at field concentrations of tens to hundreds of nanograms per liter.
Keywords	adsorption ; analytical chemistry ; cyclodextrins ; dissolved organic carbon ; isotope fractionation ; isotopes ; liquid chromatography ; pollutants ; solid phase extraction ; sorbents ; tandem mass spectrometry ; water pollution
Language	English
Dates of publication	2023-0511
Size	p. 7839-7848.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c05465
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 4' 52/94: Show issues
Z 2.9: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Standard addition method for rapid, cultivation-independent quantification of

Schwaiger, Gerhard / Matt, Marco / Streich, Philipp / Bromann, Sarah / Clauß, Marcus / Elsner, Martin / Seidel, Michael

The Analyst

2024

Abstract: Cultivation-independent molecular biological methods are essential to rapidly quantify pathogens ... ...

Abstract	Cultivation-independent molecular biological methods are essential to rapidly quantify pathogens like
Language	English
Publishing date	2024-04-11
Publishing country	England
Document type	Journal Article
ZDB-ID	210747-8
ISSN	1364-5528 ; 0003-2654
ISSN (online)	1364-5528
ISSN	0003-2654
DOI	10.1039/d3an02207b
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2423: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: TUM-ParticleTyper 2: automated quantitative analysis of (microplastic) particles and fibers down to 1 [Formula: see text]m by Raman microspectroscopy.

Jacob, Oliver / Ramírez-Piñero, Alejandro / Elsner, Martin / Ivleva, Natalia P

Analytical and bioanalytical chemistry

2023 Volume 415, Issue 15, Page(s) 2947–2961

Abstract: Accurate quantification of small microplastics in environmental and food samples is a prerequisite for studying their potential hazard. Knowledge of numbers, size distributions and polymer type for particles and fibers is particularly relevant in this ... ...

Abstract	Accurate quantification of small microplastics in environmental and food samples is a prerequisite for studying their potential hazard. Knowledge of numbers, size distributions and polymer type for particles and fibers is particularly relevant in this respect. Raman microspectroscopy can identify particles down to 1 [Formula: see text]m in diameter. Here, a fully automated procedure for quantifying microplastics across the entire defined size range is presented as the core of the new software TUM-ParticleTyper 2. This software implements the theoretical approaches of random window sampling and on-the-fly confidence interval estimation during ongoing measurements. It also includes improvements to image processing and fiber recognition (when compared to the previous software TUM-ParticleTyper for analysis of particles/fibers [Formula: see text] [Formula: see text]m), and a new approach to adaptive de-agglomeration. Repeated measurements of internally produced secondary reference microplastics were evaluated to assess the precision of the whole procedure.
Language	English
Publishing date	2023-06-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-023-04712-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 69/97: Show issues
Archiv 3: Show issues
Z 9.4: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Immunomagnetic separation coupled with flow cytometry for the analysis of Legionella pneumophila in aerosols.

Heining, Lena / Welp, Laura / Hugo, Achim / Elsner, Martin / Seidel, Michael

Analytical and bioanalytical chemistry

2023 Volume 415, Issue 21, Page(s) 5139–5149

Abstract: Legionella pneumophila are pathogenic bacteria that can be found in high concentrations in artificial water systems like evaporative cooling towers, which have been the source of frequent outbreaks in recent years. Since inhaled L. pneumophila can lead ... ...

Abstract	Legionella pneumophila are pathogenic bacteria that can be found in high concentrations in artificial water systems like evaporative cooling towers, which have been the source of frequent outbreaks in recent years. Since inhaled L. pneumophila can lead to Legionnaires' disease, the development of suitable sampling and rapid analysis strategies for these bacteria in aerosols is therefore of great relevance. In this work, different concentrations of viable L. pneumophila Sg 1 were nebulized and sampled by the cyclone sampler Coriolis® µ under defined conditions in a bioaerosol chamber. To quantify intact Legionella cells, the collected bioaerosols were subsequently analyzed by immunomagnetic separation coupled with flow cytometry (IMS-FCM) on the platform rqmicro.COUNT. For analytical comparison, measurements with qPCR and cultivation were performed. Limits of detection (LOD) of 2.9 × 10
MeSH term(s)	Humans ; Legionella pneumophila ; Immunomagnetic Separation/methods ; Flow Cytometry ; Respiratory Aerosols and Droplets ; Legionnaires' Disease/microbiology ; Water Microbiology
Language	English
Publishing date	2023-05-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-023-04738-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 69/97: Show issues
Archiv 3: Show issues
Z 9.4: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Flow-Based Chemiluminescence Microarrays as Screening Platform for Affinity Binders to Capture and Elute Bacteria.

Neumair, Julia / Elsner, Martin / Seidel, Michael

Sensors (Basel, Switzerland)

2022 Volume 22, Issue 22

Abstract: Affinity describes the non-covalent but selective interaction between an affinity binder (e.g., proteins, antibiotics, or antibodies) and its counterpart (e.g., bacteria). These affinity binders can serve to detect bacteria and respond to the need for ... ...

Abstract	Affinity describes the non-covalent but selective interaction between an affinity binder (e.g., proteins, antibiotics, or antibodies) and its counterpart (e.g., bacteria). These affinity binders can serve to detect bacteria and respond to the need for selective concentration via affinity chromatography for trace analysis. By changing the pH value or salt and protein contents, affinity bindings can be reversed, and bacteria can be recovered for characterisation. Analytical microarrays use multiple affinity binders immobilised on the surface in a distinct pattern, which immensely reduces screening time for the discovery of superior binding motifs. Here, flow-based microarray systems can inform not only about binding, but also about desorption. In this work, we pioneer a screening assay for affinity binders against both gram-positive and negative bacteria based on an automated flow-based chemiluminescence (CL) microarray. Biotinylation of model organisms
MeSH term(s)	Luminescence ; Escherichia coli ; Polymyxin B ; Microarray Analysis ; Antibodies ; Anti-Bacterial Agents
Chemical Substances	Polymyxin B (J2VZ07J96K) ; Antibodies ; Anti-Bacterial Agents
Language	English
Publishing date	2022-11-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s22228606
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito