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  1. Article ; Online: A Glossary for Chemical Approaches towards Unlocking the Trove of Metabolic Treasures in Actinomycetes

    Jianye Zhang / Heba Ali Hassan / Usama Ramadan Abdelmohsen / Eman Maher Zahran

    Molecules, Vol 27, Iss 142, p

    2022  Volume 142

    Abstract: Actinobacterial natural products showed a critical basis for the discovery of new antibiotics as well as other lead secondary metabolites. Varied environmental and physiological signals touch the antibiotic machinery that faced a serious decline in the ... ...

    Abstract Actinobacterial natural products showed a critical basis for the discovery of new antibiotics as well as other lead secondary metabolites. Varied environmental and physiological signals touch the antibiotic machinery that faced a serious decline in the last decades. The reason was exposed by genomic sequencing data, which revealed that Actinomycetes harbor a large portion of silent biosynthetic gene clusters in their genomes that encrypt for secondary metabolites. These gene clusters are linked with a great reservoir of yet unknown molecules, and arranging them is considered a major challenge for biotechnology approaches. In the present paper, we discuss the recent strategies that have been taken to augment the yield of secondary metabolites via awakening these cryptic genes in Actinomycetes with emphasis on chemical signaling molecules used to induce the antibiotics biosynthesis. The rationale, types, applications and mechanisms are discussed in detail, to reveal the productive path for the unearthing of new metabolites, covering the literature until the end of 2020.
    Keywords Actinomycetes ; antibiotics biosynthesis ; cryptic genes ; elicitors ; biological potential ; Organic chemistry ; QD241-441
    Subject code 500 ; 540
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Wound Restorative Power of Halimeda macroloba / Mesenchymal Stem Cells in Immunocompromised Rats via Downregulating Inflammatory/Immune Cross Talk

    Eman Maher Zahran / Reham H. Mohyeldin / Fatma Mohamed Abd El-Mordy / Sherif A. Maher / Omar Y. Tammam / Entesar Ali Saber / Faisal H. Altemani / Naseh A. Algehainy / Mohammad A. Alanazi / Mohammed M. Jalal / Mahmoud A. Elrehany / Usama Ramadan Abdelmohsen

    Marine Drugs, Vol 21, Iss 336, p

    2023  Volume 336

    Abstract: Impaired skin wound healing is still a major challenge, especially with immunocompromised patients who express delayed healing and are susceptible to infections. Injection of rat-derived bone marrow mesenchymal stem cells (BMMSCs) via the tail vein ... ...

    Abstract Impaired skin wound healing is still a major challenge, especially with immunocompromised patients who express delayed healing and are susceptible to infections. Injection of rat-derived bone marrow mesenchymal stem cells (BMMSCs) via the tail vein accelerates cutaneous wound healing via their paracrine activity. The present work aimed to investigate the combined wound-healing potential of BMMSCs and Halimeda macroloba algae extract in immunocompromised rats. High-resolution liquid chromatography-mass spectrometry (HR-LC-MS) investigation of the extract revealed the presence of variant phytochemicals, mostly phenolics, and terpenoids, known for their angiogenic, collagen-stimulating, anti-inflammatory, and antioxidant properties. The BMMSCs were isolated and characterized for CD markers, where they showed a positive expression of CD90 by 98.21% and CD105 by 97.1%. Twelve days after inducing immunocompromise (40 mg/kg hydrocortisone daily), a circular excision was created in the dorsal skin of rats and the treatments were continued for 16 days. The studied groups were sampled on days 4, 8, 12, and 16 after wounding. The gross/histopathological results revealed that the wound closure (99%), thickness, density of new epidermis and dermis, and skin elasticity in the healed wounds were considerably higher in the BMMSCs/ Halimeda group than the control group ( p < 0.05). RT-PCR gene expression analysis revealed that the BMMSCs/ Halimeda extract combination had perfectly attenuated oxidative stress, proinflammatory cytokines, and NF- K B activation at day 16 of wounding. The combination holds promise for regenerative medicine, representing a revolutionary step in the wound healing of immunocompromised patients, with still a need for safety assessments and further clinical trials.
    Keywords Halimeda macroloba ; stem cells ; wound healing ; inflammatory mediators ; immunocompromise ; Biology (General) ; QH301-705.5
    Subject code 610
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: New Halogenated Compounds from Halimeda macroloba Seaweed with Potential Inhibitory Activity against Malaria

    Abeer H. Elmaidomy / Eman Maher Zahran / Raya Soltane / Ahlam Alasiri / Hani Saber / Che Julius Ngwa / Gabriele Pradel / Faisal Alsenani / Ahmed M. Sayed / Usama Ramadan Abdelmohsen

    Molecules, Vol 27, Iss 5617, p

    2022  Volume 5617

    Abstract: Malaria is one of the most important infectious diseases worldwide. The causative of the most severe forms of malaria, Plasmodium falciparum , has developed resistances against all the available antimalarial drugs. In the present study, the phytochemical ...

    Abstract Malaria is one of the most important infectious diseases worldwide. The causative of the most severe forms of malaria, Plasmodium falciparum , has developed resistances against all the available antimalarial drugs. In the present study, the phytochemical investigation of the green seaweed Halimeda macroloba has afforded two new compounds 1 – 2 , along with 4 known ones 3 – 6 . The structures of the compounds had been confirmed using 1& 2D-NMR and HRESIMS analyses. Extensive machine-learning-supported virtual-screening suggested cytochrome-C enzyme as a potential target for compound 2 . Docking, absolute-binding-free-energy (Δ G binding ) and molecular-dynamics-simulation (MDS) of compound 2 revealed the strong binding interaction of this compound with cytochrome-C. In vitro testing for crude extract and isolated compounds revealed the potential in vitro inhibitory activity of both extract and compound 2 against P. falciparum . The crude extract was able to inhibit the parasite growth with an IC 50 value of 1.8 ± 0.35 µg/mL. Compound 2 also showed good inhibitory activity with an IC 50 value of 3.2 ± 0.23 µg/mL. Meanwhile, compound 6 showed moderate inhibitory activity with an IC 50 value of 19.3 ± 0.51 µg/mL. Accordingly, the scaffold of compound 2 can be considered as a good lead compound for the future development of new antimalarial agents.
    Keywords malaria ; Plasmodium falciparum ; Halimeda macrolaba ; cytochrome C ; docking ; molecular dynamics ; Organic chemistry ; QD241-441
    Subject code 500
    Language English
    Publishing date 2022-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Bioactivity Potential of Marine Natural Products from Scleractinia-Associated Microbes and In Silico Anti-SARS-COV-2 Evaluation

    Eman Maher Zahran / Amgad Albohy / Amira Khalil / Alyaa Hatem Ibrahim / Heba Ali Ahmed / Ebaa M. El-Hossary / Gerhard Bringmann / Usama Ramadan Abdelmohsen

    Marine Drugs, Vol 18, Iss 645, p

    2020  Volume 645

    Abstract: Marine organisms and their associated microbes are rich in diverse chemical leads. With the development of marine biotechnology, a considerable number of research activities are focused on marine bacteria and fungi-derived bioactive compounds. Marine ... ...

    Abstract Marine organisms and their associated microbes are rich in diverse chemical leads. With the development of marine biotechnology, a considerable number of research activities are focused on marine bacteria and fungi-derived bioactive compounds. Marine bacteria and fungi are ranked on the top of the hierarchy of all organisms, as they are responsible for producing a wide range of bioactive secondary metabolites with possible pharmaceutical applications. Thus, they have the potential to provide future drugs against challenging diseases, such as cancer, a range of viral diseases, malaria, and inflammation. This review aims at describing the literature on secondary metabolites that have been obtained from Scleractinian-associated organisms including bacteria, fungi, and zooxanthellae, with full coverage of the period from 1982 to 2020, as well as illustrating their biological activities and structure activity relationship (SAR). Moreover, all these compounds were filtered based on ADME analysis to determine their physicochemical properties, and 15 compounds were selected. The selected compounds were virtually investigated for potential inhibition for SARS-CoV-2 targets using molecular docking studies. Promising potential results against SARS-CoV-2 RNA dependent RNA polymerase (RdRp) and methyltransferase (nsp16) are presented.
    Keywords Scleractinia ; marine bacteria ; marine fungi ; zooxanthellae ; marine natural products ; ADME analysis ; Biology (General) ; QH301-705.5
    Subject code 540 ; 333
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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