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  1. Book ; Online ; E-Book: Pathological specimens and genomic medicine

    Tangrea, Michael A. / Rosenberg, Avi Z. / Emmert-Buck, Michael R.

    emerging issues

    2019  

    Author's details editors Michael A Tangrea, Avi Z Rosenberg, Michael R Emmert-Buck
    Keywords Pathology, Molecular / methods ; Genomics / methods
    Language English
    Size 1 Online-Ressource (ix, 370 Seiten), Illustrationen
    Publisher World Scientific
    Publishing place New Jersey
    Publishing country United States
    Document type Book ; Online ; E-Book
    Note Includes bibliographical references and index
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT020116647
    ISBN 9789813276239 ; 9789813276222 ; 9813276231 ; 9813276223
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book: Dissecting the molecular anatomy of tissue

    Emmert-Buck, Michael R.

    with 8 tables

    (Principles and practice)

    2005  

    Author's details Michael R. Emmert-Buck ... (ed.)
    Series title Principles and practice
    Keywords Krebsforschung ; Molekulare Diagnostik ; Gewebe ; Dissektion
    Subject Dissectio ; Molekulardiagnostik ; Krebs ; Onkologie ; Tumorbiologie
    Language English
    Size XVII, 224 S. : Ill., graph. Darst., 235 mm x 155 mm
    Publisher Springer
    Publishing place Berlin u.a.
    Publishing country Germany
    Document type Book
    HBZ-ID HT014220234
    ISBN 3-540-22006-2 ; 978-3-540-22006-0
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2004 A 5325 order for loan Magazin

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  3. Article ; Online: An NIH intramural percubator as a model of academic-industry partnerships: from the beginning of life through the valley of death.

    Emmert-Buck, Michael R

    Journal of translational medicine

    2011  Volume 9, Page(s) 54

    Abstract: In 2009 the NIH publicly announced five strategic goals for the institutes that included the critical need to translate research discoveries into public benefit at an accelerated pace, with a commitment to find novel ways to engage academic investigators ...

    Abstract In 2009 the NIH publicly announced five strategic goals for the institutes that included the critical need to translate research discoveries into public benefit at an accelerated pace, with a commitment to find novel ways to engage academic investigators in the process. The emphasis on moving scientific advancements from the laboratory to the clinic is an opportune time to discuss how the NIH intramural program in Bethesda, the largest biomedical research center in the world, can participate in this endeavor. Proposed here for consideration is a percolator-incubator program, a 'percubator' designed to enable NIH intramural investigators to develop new medical interventions as quickly and efficiently as possible.
    MeSH term(s) Cooperative Behavior ; Industry ; Models, Theoretical ; National Institutes of Health (U.S.) ; Translational Medical Research ; United States ; Universities
    Language English
    Publishing date 2011-05-08
    Publishing country England
    Document type Editorial
    ISSN 1479-5876
    ISSN (online) 1479-5876
    DOI 10.1186/1479-5876-9-54
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: An NIH intramural percubator as a model of academic-industry partnerships

    Emmert-Buck Michael R

    Journal of Translational Medicine, Vol 9, Iss 1, p

    from the beginning of life through the valley of death

    2011  Volume 54

    Abstract: Abstract In 2009 the NIH publicly announced five strategic goals for the institutes that included the critical need to translate research discoveries into public benefit at an accelerated pace, with a commitment to find novel ways to engage academic ... ...

    Abstract Abstract In 2009 the NIH publicly announced five strategic goals for the institutes that included the critical need to translate research discoveries into public benefit at an accelerated pace, with a commitment to find novel ways to engage academic investigators in the process. The emphasis on moving scientific advancements from the laboratory to the clinic is an opportune time to discuss how the NIH intramural program in Bethesda, the largest biomedical research center in the world, can participate in this endeavor. Proposed here for consideration is a percolator-incubator program, a 'percubator' designed to enable NIH intramural investigators to develop new medical interventions as quickly and efficiently as possible.
    Keywords Medicine ; R
    Language English
    Publishing date 2011-05-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.

    Johann, Donald J / Shin, Ik Jae / Roberge, Adam / Laun, Sarah / Peterson, Erich A / Liu, Meei / Steliga, Matthew A / Muesse, Jason / Emmert-Buck, Michael R / Tangrea, Michael A

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

    2022  Volume 70, Issue 9, Page(s) 643–658

    Abstract: Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue ... ...

    Abstract Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (
    MeSH term(s) Antigens ; DNA/analysis ; Epitopes ; Genomics ; Humans ; Lung Neoplasms/genetics ; Mutation ; Peptide Hydrolases ; Proto-Oncogene Proteins p21(ras)/genetics
    Chemical Substances Antigens ; Epitopes ; DNA (9007-49-2) ; Peptide Hydrolases (EC 3.4.-) ; Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2)
    Language English
    Publishing date 2022-09-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 218208-7
    ISSN 1551-5044 ; 0022-1554
    ISSN (online) 1551-5044
    ISSN 0022-1554
    DOI 10.1369/00221554221124163
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.321: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision Medicine.

    Johann, Donald J / Laun, Sarah / Shin, Ik Jae / Weigman, Robert / Stephens, Owen / Roberge, Adam / Liu, Meeiyueh / Greisman, Valerie / Steliga, Mathew / Muesse, Jason / Peterson, Erich / Emmert-Buck, Michael R / Tangrea, Michael A

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2394, Page(s) 93–107

    Abstract: Improving the utilization of tumor tissue from diagnostic biopsies is an unmet medical need. This is especially relevant today in the rapidly evolving precision oncology field where tumor genotyping is often essential for the indication of many advanced ... ...

    Abstract Improving the utilization of tumor tissue from diagnostic biopsies is an unmet medical need. This is especially relevant today in the rapidly evolving precision oncology field where tumor genotyping is often essential for the indication of many advanced and targeted therapies. National Comprehensive Cancer Network (NCCN) guidelines now mandate molecular testing for clinically actionable targets in certain malignancies. Utilizing advanced stage lung cancer as an example, an improved genotyping approach for solid tumors is possible. The strategy involves optimization of the microdissection process and analysis of a large number of identical target cells from formalin-fixed paraffin-embedded (FFPE) specimens sharing similar characteristics, in other words, single-cell subtype analysis. The shared characteristics can include immunostaining status, cell phenotype, and/or spatial location within a histological section. Synergy between microdissection and droplet digital PCR (ddPCR) enhances the molecular analysis. We demonstrate here a methodology that illustrates genotyping of a solid tumor from a small tissue biopsy sample in a time- and cost-efficient manner, using immunostain targeting as an example.
    MeSH term(s) Formaldehyde ; Humans ; Microdissection/methods ; Neoplasms ; Paraffin Embedding/methods ; Polymerase Chain Reaction/methods ; Precision Medicine ; Tissue Fixation/methods
    Chemical Substances Formaldehyde (1HG84L3525)
    Language English
    Publishing date 2022-01-31
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1811-0_7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Book: Dissecting the molecular anatomy of tissue

    Emmert-Buck, Michael R

    with 8 tables

    (Principles and practice)

    2005  

    Abstract: The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of ... ...

    Author's details Michael R. Emmert-Buck, ... (eds.)
    Series title Principles and practice
    Abstract The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of handling clinical tissue samples, including the impact of handling on subsequent molecular analysis, are also discussed. In addition, detailed protocols for molecular analysis of DNA, RNA and protein, with special emphasis on molecular analysis of highly complex human tissue samples containing mixtures of cell populations, are provided. TOC:I Clinical Issues and Tissue Processing: Molecular Pathology Laboratory of the Future; Clinical Utilities of Microdissected Samples; Tissue Processing; Sample Management and Tracking.- II Methods of Tissue Microanalysis: Tissue Microarrays.- III Molecular Analysis of Micodissected Samples: Methods of Microdissection and New Technologies; Standard Diagnostic Techniques; DNA Applications; mRNA Applications; Proteomic Applications; Protocols for Tissue Handling and Molecular Analysis
    Keywords Krebsforschung ; Gewebe ; Dissektion ; Molekulare Diagnostik
    Language English
    Size X, 214 S, Ill., graph. Darst, 23 cm
    Publisher Springer
    Publishing place Berlin u.a.
    Document type Book
    ISBN 3540220062 ; 9783540220060
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  8. Article ; Online: Laser Microdissection.

    Frost, Andra R / Eltoum, Isam-Eldin / Siegal, Gene P / Emmert-Buck, Michael R / Tangrea, Michael A

    Current protocols in molecular biology

    2015  Volume 112, Page(s) 25A.1.1–25A.1.30

    Abstract: Laser microdissection (LM) offers a relatively rapid and precise method of isolating and removing specified cells from complex tissues for subsequent analysis of their RNA, DNA, protein or metabolite content, thereby allowing assessment of the role of ... ...

    Abstract Laser microdissection (LM) offers a relatively rapid and precise method of isolating and removing specified cells from complex tissues for subsequent analysis of their RNA, DNA, protein or metabolite content, thereby allowing assessment of the role of different cell types in the normal physiological or disease processes being studied. In this unit, protocols for the preparation of mammalian frozen tissues, fixed tissues, and cytologic specimens for LM, including tissue freezing, tissue processing and paraffin embedding, histologic sectioning, cell processing, hematoxylin and eosin staining, immunohistochemistry, and image-guided cell targeting are presented. Also provided are recipes for generating lysis buffers for the recovery of nucleic acids and proteins. The Commentary section addresses the types of specimens that can be utilized for LM and approaches to staining of specimens for cell visualization. Emphasis is placed on the preparation of tissue or cytologic specimens as this is critical to effective LM.
    MeSH term(s) Animals ; Humans ; Laser Capture Microdissection/methods ; Mammals ; Single-Cell Analysis/methods ; Specimen Handling/methods
    Language English
    Publishing date 2015-10-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural ; Research Support, Non-U.S. Gov't
    ISSN 1934-3647
    ISSN (online) 1934-3647
    DOI 10.1002/0471142727.mb25a01s112
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Proteomic profiling of cancer--opportunities, challenges, and context.

    Arnott, David / Emmert-Buck, Michael R

    The Journal of pathology

    2010  Volume 222, Issue 1, Page(s) 16–20

    Abstract: The article by Roesch-Ely and colleagues in a recent issue of The Journal of Pathology describes the use of proteomic techniques to examine mucosal biopsies in patients with head and neck squamous cell cancer (HNSCC) and in corresponding control samples. ...

    Abstract The article by Roesch-Ely and colleagues in a recent issue of The Journal of Pathology describes the use of proteomic techniques to examine mucosal biopsies in patients with head and neck squamous cell cancer (HNSCC) and in corresponding control samples. The authors were able to determine the anatomical site of origin of the biopsies based on modelling of multiplex protein datasets, and to use the information to analyse field cancerization as a means of predicting tumour recurrence. Although the study included only a relatively small number of cases, and will require future validation in a larger patient cohort, the results point to the potential of proteomics to increase our understanding of cancer biology, and in this instance to offer clinical value.
    MeSH term(s) Biomarkers, Tumor/metabolism ; Carcinoma, Squamous Cell/metabolism ; Head and Neck Neoplasms/metabolism ; Humans ; Neoplasm Proteins/metabolism ; Neoplasms/metabolism ; Proteomics/methods ; Proteomics/trends
    Chemical Substances Biomarkers, Tumor ; Neoplasm Proteins
    Language English
    Publishing date 2010-09
    Publishing country England
    Document type Comment ; Journal Article
    ZDB-ID 3119-7
    ISSN 1096-9896 ; 0022-3417
    ISSN (online) 1096-9896
    ISSN 0022-3417
    DOI 10.1002/path.2750
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.12: Show issues Location:
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  10. Article: Proteomic profiling of cancer--opportunities, challenges, and context

    Arnott, David / Emmert-Buck, Michael R

    Journal of pathology. 2010 Sept., v. 222, no. 1

    2010  

    Abstract: The article by Roesch-Ely and colleagues in a recent issue of The Journal of Pathology describes the use of proteomic techniques to examine mucosal biopsies in patients with head and neck squamous cell cancer (HNSCC) and in corresponding control samples. ...

    Abstract The article by Roesch-Ely and colleagues in a recent issue of The Journal of Pathology describes the use of proteomic techniques to examine mucosal biopsies in patients with head and neck squamous cell cancer (HNSCC) and in corresponding control samples. The authors were able to determine the anatomical site of origin of the biopsies based on modelling of multiplex protein datasets, and to use the information to analyse field cancerization as a means of predicting tumour recurrence. Although the study included only a relatively small number of cases, and will require future validation in a larger patient cohort, the results point to the potential of proteomics to increase our understanding of cancer biology, and in this instance to offer clinical value. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
    Language English
    Dates of publication 2010-09
    Size p. 16-20.
    Publishing place John Wiley & Sons, Ltd.
    Document type Article
    ZDB-ID 3119-7
    ISSN 1096-9896 ; 0022-3417
    ISSN (online) 1096-9896
    ISSN 0022-3417
    DOI 10.1002/path.2750
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 66/40: Show issues

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