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Article ; Online: Grass Pea

Sarkar, Abhimanyu / Emmrich, Peter M.F. / Sarker, Ashutosh / Xuxiao Zong / Martin, Cathie / Wang, Trevor L.

Genomic Designing of Climate-Smart Pulse Crops

Remodeling an Ancient Insurance Crop for Climate Resilience

2023

Keywords	climate ; resilience ; insurance ; pea
Publishing date	2023-03-10T14:33:32Z
Publisher	Springer
Publishing country	fr
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines

Emmrich, Peter M.F. / Rejzek, Martin / Hill, Lionel / Brett, Paul / Edwards, Anne / Sarkar, Abhimanyu / Field, Rob A. / Martin, Cathie / Wang, Trevor L.

BMC Plant Biology

2023

Abstract: Grass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3- ... ...

Abstract	Grass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties.
Keywords	identification ; permits ; pea
Publishing date	2023-03-10T14:41:21Z
Publisher	Springer
Publishing country	fr
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives.

Aloui, Khawla / Choukri, Hasnae / El Haddad, Noureddine / Gupta, Priyanka / El Bouhmadi, Keltoum / Emmrich, Peter M F / Singh, Akanksha / Edwards, Anne / Maalouf, Fouad / Bouhlal, Outmane / Staples, Jasmine / Kumar, Shiv

Plants (Basel, Switzerland)

2023 Volume 12, Issue 19

Abstract: Grasspea ( ...

Abstract	Grasspea (
Language	English
Publishing date	2023-10-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants12193501
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Chromosome-level genome assembly and population genomic resource to accelerate orphan crop lablab breeding.

Njaci, Isaac / Waweru, Bernice / Kamal, Nadia / Muktar, Meki Shehabu / Fisher, David / Gundlach, Heidrun / Muli, Collins / Muthui, Lucy / Maranga, Mary / Kiambi, Davies / Maass, Brigitte L / Emmrich, Peter M F / Domelevo Entfellner, Jean-Baka / Spannagl, Manuel / Chapman, Mark A / Shorinola, Oluwaseyi / Jones, Chris S

Nature communications

2023 Volume 14, Issue 1, Page(s) 1915

Abstract: Under-utilised orphan crops hold the key to diversified and climate-resilient food systems. Here, we report on orphan crop genomics using the case of Lablab purpureus (L.) Sweet (lablab) - a legume native to Africa and cultivated throughout the tropics ... ...

Abstract	Under-utilised orphan crops hold the key to diversified and climate-resilient food systems. Here, we report on orphan crop genomics using the case of Lablab purpureus (L.) Sweet (lablab) - a legume native to Africa and cultivated throughout the tropics for food and forage. Our Africa-led plant genome collaboration produces a high-quality chromosome-scale assembly of the lablab genome. Our assembly highlights the genome organisation of the trypsin inhibitor genes - an important anti-nutritional factor in lablab. We also re-sequence cultivated and wild lablab accessions from Africa confirming two domestication events. Finally, we examine the genetic and phenotypic diversity in a comprehensive lablab germplasm collection and identify genomic loci underlying variation of important agronomic traits in lablab. The genomic data generated here provide a valuable resource for lablab improvement. Our inclusive collaborative approach also presents an example that can be explored by other researchers sequencing indigenous crops, particularly from low and middle-income countries (LMIC).
MeSH term(s)	Metagenomics ; Plant Breeding ; Crops, Agricultural/genetics ; Genome, Plant/genetics ; Fabaceae/genetics ; Chromosomes
Language	English
Publishing date	2023-04-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-37489-7
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Article ; Online: Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines.

Emmrich, Peter M F / Rejzek, Martin / Hill, Lionel / Brett, Paul / Edwards, Anne / Sarkar, Abhimanyu / Field, Rob A / Martin, Cathie / Wang, Trevor L

BMC plant biology

2019 Volume 19, Issue 1, Page(s) 489

Abstract: Background: Grass pea (Lathyrus sativus) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3- ...

Abstract	Background: Grass pea (Lathyrus sativus) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties. Results: A plate assay, based on an established spectrophotometric method enabling high-throughput ODAP measurements, is described. In addition, we describe a novel liquid chromatography mass spectrometry (LCMS)-based method for β-L-ODAP-quantification. This method utilises an internal standard (di- Conclusions: The plate-based spectrophotometric assay allows quantification of total ODAP in large numbers of samples, but its low sensitivity and inability to differentiate α- and β-L-ODAP limit its usefulness for accurate quantification in low-ODAP samples. Coupled to the use of a stable isotope internal standard with LCMS that allows accurate quantification of β-L-ODAP in grass pea samples with high sensitivity, these methods permit the identification and characterisation of grass pea lines with a very low ODAP content. The LCMS method is offered as a new 'gold standard' for β-L-ODAP quantification, especially for the validation of existing and novel low- and/or zero-β-L-ODAP genotypes.
MeSH term(s)	Amino Acids, Diamino/analysis ; Chromatography, Liquid/economics ; Chromatography, Liquid/methods ; Costs and Cost Analysis ; Isotope Labeling ; Lathyrus/chemistry ; Lathyrus/genetics ; Mass Spectrometry/economics ; Mass Spectrometry/methods ; Neurotoxins/analysis ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrophotometry/economics ; Spectrophotometry/methods ; Time Factors
Chemical Substances	Amino Acids, Diamino ; Neurotoxins ; oxalyldiaminopropionic acid (1TG777QI25)
Language	English
Publishing date	2019-11-12
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	2059868-3
ISSN	1471-2229 ; 1471-2229
ISSN (online)	1471-2229
ISSN	1471-2229
DOI	10.1186/s12870-019-2091-5
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Article: Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines

Emmrich, Peter M. F / Rejzek, Martin / Hill, Lionel / Brett, Paul / Edwards, Anne / Sarkar, Abhimanyu / Field, Rob A / Martin, Cathie / Wang, Trevor L

BMC plant biology. 2019 Dec., v. 19, no. 1

2019

Abstract: BACKGROUND: Grass pea (Lathyrus sativus) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3- ... ...

Abstract	BACKGROUND: Grass pea (Lathyrus sativus) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties. RESULTS: A plate assay, based on an established spectrophotometric method enabling high-throughput ODAP measurements, is described. In addition, we describe a novel liquid chromatography mass spectrometry (LCMS)-based method for β-L-ODAP-quantification. This method utilises an internal standard (di-13C-labelled β-L-ODAP) allowing accurate quantification of β-L-ODAP in grass pea tissue samples. The synthesis of this standard is also described. The two methods are compared; the spectrophotometric assay lacked sensitivity and detected ODAP-like absorbance in chickpea and pea whereas the LCMS method did not detect any β-L-ODAP in these species. The LCMS method was also used to quantify β-L-ODAP accurately in different tissues of grass pea. CONCLUSIONS: The plate-based spectrophotometric assay allows quantification of total ODAP in large numbers of samples, but its low sensitivity and inability to differentiate α- and β-L-ODAP limit its usefulness for accurate quantification in low-ODAP samples. Coupled to the use of a stable isotope internal standard with LCMS that allows accurate quantification of β-L-ODAP in grass pea samples with high sensitivity, these methods permit the identification and characterisation of grass pea lines with a very low ODAP content. The LCMS method is offered as a new ‘gold standard’ for β-L-ODAP quantification, especially for the validation of existing and novel low- and/or zero-β-L-ODAP genotypes.
Keywords	Lathyrus sativus ; absorbance ; chickpeas ; climate change ; drought ; food security ; genotype ; germplasm ; liquid chromatography ; mass spectrometry ; neurotoxins ; new variety ; peas ; rapid methods ; screening ; spectrophotometers ; stable isotopes ; staple crops ; tissues
Language	English
Dates of publication	2019-12
Size	p. 489.
Publishing place	BioMed Central
Document type	Article
ISSN	1471-2229
DOI	10.1186/s12870-019-2091-5
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus.

Edwards, Anne / Njaci, Isaac / Sarkar, Abhimanyu / Jiang, Zhouqian / Kaithakottil, Gemy George / Moore, Christopher / Cheema, Jitender / Stevenson, Clare E M / Rejzek, Martin / Novák, Petr / Vigouroux, Marielle / Vickers, Martin / Wouters, Roland H M / Paajanen, Pirita / Steuernagel, Burkhard / Moore, Jonathan D / Higgins, Janet / Swarbreck, David / Martens, Stefan /

Kim, Colin Y / Weng, Jing-Ke / Mundree, Sagadevan / Kilian, Benjamin / Kumar, Shiv / Loose, Matt / Yant, Levi / Macas, Jiří / Wang, Trevor L / Martin, Cathie / Emmrich, Peter M F

Nature communications

2023 Volume 14, Issue 1, Page(s) 876

Abstract: Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing ...

Abstract	Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop's association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.
MeSH term(s)	Lathyrus/genetics ; Lathyrus/metabolism ; Amino Acids, Diamino/metabolism ; Neurotoxins/metabolism ; Genomics
Chemical Substances	oxalyldiaminopropionic acid (1TG777QI25) ; Amino Acids, Diamino ; Neurotoxins
Language	English
Publishing date	2023-02-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-36503-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Author Correction: Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus.

Kim, Colin Y / Weng, Jing-Ke / Mundree, Sagadevan / Kilian, Benjamin / Kumar, Shiv / Loose, Matt / Yant, Levi / Macas, Jiří / Wang, Trevor L / Martin, Cathie / Emmrich, Peter M F

Nature communications

2023 Volume 14, Issue 1, Page(s) 5199

Language	English
Publishing date	2023-08-25
Publishing country	England
Document type	Published Erratum
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-40984-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A Mobile Health Wallet for pregnancy-related healthcare in Madagascar

Muller, N. / Emmrich, Peter M.F. / Rajemison, E.N. / Neve, J.W. de / Bärnighausen, T. / Knauss, S. / Emmrich, J.V.

JMIR Mhealth Uhealth

A mixed-methods study on opportunities and challenges

2019

Keywords	gender ; health ; women
Language	English
Publishing date	2019-01-02T11:23:10Z
Publisher	JMIR Publications Inc.
Publishing country	fr
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus

Edwards, Anne / Njaci, Isaac / Sarkar, Abhimanyu / Jiang, Zhouqian / Kaithakottil, Gemy George / Moore, Christopher / Cheema, Jitender / Stevenson, Clare E.M. / Rejzek, Martin / Novák, Petr / Vigouroux, Marielle / Vickers, Martin / Wouters, Roland H.M. / Paajanen, Pirita / Steuernagel, Burkhard / Moore, Jonathan D. / Higgins, Janet / Swarbreck, David / Martens, Stefan /

Kim, Colin Y. / Weng, Jing-Ke / Mundree, Sagadevan / Kilian, Benjamin / Kumar, Shiv / Loose, Matt / Yant, Levi / Macas, Jiří / Wang, Trevor L. / Martin, Cathie / Emmrich, Peter M.F.

Nature Communications

2023

Abstract: Grass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a ... ...

Abstract	Grass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.
Keywords	genomics ; biosynthesis ; lathyrus
Publishing date	2023-03-10T14:38:58Z
Publisher	Springer
Publishing country	fr
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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