Article: [Simultaneous determination of eight organic acids in Fructus mume by RP-HPLC].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
2006 Volume 31, Issue 21, Page(s) 1783–1786
Abstract: Objective: To develop an HPLC method for the simultaneous separation and determination of oxalic acid (OA), tartaric acid(TA), malic acid(MA), vitamin C (VC), lactic acid (LA), acetic acid (AA) citric acid (CA) and succinic acid (SA) in Fructus mume.: ...
| Abstract | Objective: To develop an HPLC method for the simultaneous separation and determination of oxalic acid (OA), tartaric acid(TA), malic acid(MA), vitamin C (VC), lactic acid (LA), acetic acid (AA) citric acid (CA) and succinic acid (SA) in Fructus mume. Method: Analytical column was Zorbax Eclipse XDB C18. Mobile phase was 0.5% (NH4) H2PO4 aqueous solution and detection wavelength was 214 nm. The flow rate of mobile phase was 0.5 mL x min(-1). Result: The regression equations (pH 2. 8, adjusted with phosphoric acid) of eight constituents have been established, r = 0.999 7, 0. 999 8, 0.999 2, 0.999 6, 0.999 1, 0.999 5, 0.999 8, 0.999 2 respectively. Meanwhile, the content and proportion relationship of eight organic acids in Fructus mume which yielded in Fujian (China) were investigated. Conclusion: This method was simple, accuracy and quick. The method can be used for the purpose of routine analysis and the quality control of a botanic (Fructus mume) containing these organic acid components. |
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| MeSH term(s) | Acetic Acid/analysis ; Ascorbic Acid/analysis ; Carboxylic Acids/analysis ; Chromatography, High Pressure Liquid/methods ; Citric Acid/analysis ; Fruit/chemistry ; Lactic Acid/analysis ; Malates/analysis ; Oxalic Acid/analysis ; Plants, Medicinal/chemistry ; Prunus/chemistry ; Quality Control ; Reproducibility of Results ; Succinic Acid/analysis ; Tartrates/analysis | |||||
| Chemical Substances | Carboxylic Acids ; Malates ; Tartrates ; Citric Acid (2968PHW8QP) ; Lactic Acid (33X04XA5AT) ; malic acid (817L1N4CKP) ; Oxalic Acid (9E7R5L6H31) ; Succinic Acid (AB6MNQ6J6L) ; Ascorbic Acid (PQ6CK8PD0R) ; Acetic Acid (Q40Q9N063P) ; tartaric acid (W4888I119H) | |||||
| Language | Chinese | |||||
| Publishing date | 2006-11 | |||||
| Publishing country | China | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 1004649-5 | |||||
| ISSN | 1001-5302 ; 0254-0029 | |||||
| ISSN | 1001-5302 ; 0254-0029 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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