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  1. Article ; Online: A proteomic overview of the major venom components from Tityus championi from Panama.

    Salazar, Marcos H / Ortíz, Magdalena Hernández / Encarnación, Sergio / Zamudio, Fernando / Possani, Lourival D / Cleghorn, John / Morán, Maricruz / Acosta, Hildaura / Corzo, Gerardo

    Toxicon : official journal of the International Society on Toxinology

    2023  Volume 227, Page(s) 107082

    Abstract: In recent years, morbidity caused by scorpion sting of the species Tityus championi has increased in Panama. Therefore, the ... ...

    Abstract In recent years, morbidity caused by scorpion sting of the species Tityus championi has increased in Panama. Therefore, the LD
    MeSH term(s) Animals ; Mice ; Venoms/metabolism ; Scorpions/chemistry ; Proteomics ; Tandem Mass Spectrometry ; Peptides/chemistry ; Scorpion Venoms/chemistry ; Mammals/metabolism
    Chemical Substances Venoms ; Peptides ; Scorpion Venoms
    Language English
    Publishing date 2023-03-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2023.107082
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  2. Article ; Online: A proteomic overview of the major venom components from Tityus championi from Panama

    Salazar, Marcos H. / Ortíz, Magdalena Hernández / Encarnación, Sergio / Zamudio, Fernando / Possani, Lourival D. / Cleghorn, John / Morán, Maricruz / Acosta, Hildaura / Corzo, Gerardo

    Toxicon. 2023 May, v. 227 p.107082-

    2023  

    Abstract: In recent years, morbidity caused by scorpion sting of the species Tityus championi has increased in Panama. Therefore, the LD₅₀ was determined by intravenous injection in 2.9 mg/kg and the venom of T. championi was separated using a HPLC system and ... ...

    Abstract In recent years, morbidity caused by scorpion sting of the species Tityus championi has increased in Panama. Therefore, the LD₅₀ was determined by intravenous injection in 2.9 mg/kg and the venom of T. championi was separated using a HPLC system and their fractions were tested for biological activities in mice to identify the most toxic fractions to mammals. In addition, the venom fractions were also tested against invertebrates to look for insect-specific toxin peptides. The most toxic fractions were analyzed by MS/MS spectrometry. The primary structures of T. championi venom peptides with the most relevant activity were obtained, and the primary structure of one of most neurotoxic peptides was found at least in other four species of Tityus from Panama. This neurotoxin is quite important to be used as a protein target to be neutralized if developing antivenoms against the sting of this Panamanian scorpion or other relevant species of genera Tityus in the country.
    Keywords Tityus ; antivenoms ; intravenous injection ; morbidity ; neurotoxicity ; neurotoxins ; peptides ; proteomics ; spectroscopy ; venoms ; Panama ; Scorpion ; Toxicity ; Venom
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2023.107082
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  3. Article ; Online: Impact of c-di-GMP on the Extracellular Proteome of Rhizobium etli

    Lorite, María J. / Casas-Román, Ariana / Girard, Lourdes / Encarnación, Sergio / Díaz-Garrido, Natalia / Badía, Josefa / Baldomá, Laura / Pérez-Mendoza, Daniel / Sanjuán, Juan

    Biology (Basel). 2022 Dec. 26, v. 12, no. 1

    2022  

    Abstract: Extracellular matrix components of bacterial biofilms include biopolymers such as polysaccharides, nucleic acids and proteins. Similar to polysaccharides, the secretion of adhesins and other matrix proteins can be regulated by the second messenger cyclic ...

    Abstract Extracellular matrix components of bacterial biofilms include biopolymers such as polysaccharides, nucleic acids and proteins. Similar to polysaccharides, the secretion of adhesins and other matrix proteins can be regulated by the second messenger cyclic diguanylate (cdG). We have performed quantitative proteomics to determine the extracellular protein contents of a Rhizobium etli strain expressing high cdG intracellular levels. cdG promoted the exportation of proteins that likely participate in adhesion and biofilm formation: the rhizobial adhesion protein RapA and two previously undescribed likely adhesins, along with flagellins. Unexpectedly, cdG also promoted the selective exportation of cytoplasmic proteins. Nearly 50% of these cytoplasmic proteins have been previously described as moonlighting or candidate moonlighting proteins in other organisms, often found extracellularly. Western blot assays confirmed cdG-promoted export of two of these cytoplasmic proteins, the translation elongation factor (EF-Tu) and glyceraldehyde 3-phosphate dehydrogenase (Gap). Transmission Electron Microscopy immunolabeling located the Gap protein in the cytoplasm but was also associated with cell membranes and extracellularly, indicative of an active process of exportation that would be enhanced by cdG. We also obtained evidence that cdG increases the number of extracellular Gap proteoforms, suggesting a link between cdG, the post-translational modification and the export of cytoplasmic proteins.
    Keywords GTPase-activating proteins ; Rhizobium etli ; Western blotting ; adhesins ; adhesion ; biofilm ; biopolymers ; cyclic GMP ; cytoplasm ; exports ; extracellular matrix ; flagellin ; glyceraldehyde-3-phosphate dehydrogenase ; peptide elongation factors ; polysaccharides ; post-translational modification ; proteome ; proteomics ; secretion ; transmission electron microscopy
    Language English
    Dates of publication 2022-1226
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology12010044
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  4. Article: Metaproteomic Insights Into the Microbial Community in Pozol.

    Rizo, Jocelin / Guillén, Daniel / Díaz-Ruiz, Gloria / Wacher, Carmen / Encarnación, Sergio / Sánchez, Sergio / Rodríguez-Sanoja, Romina

    Frontiers in nutrition

    2021  Volume 8, Page(s) 714814

    Abstract: Pozol is an acidic, refreshing, and non-alcoholic traditional Mayan beverage made with nixtamalized corn dough that is fermented spontaneously. The extensive analysis of the microbiology, biochemistry and metaproteomics of pozol allowed the construction ... ...

    Abstract Pozol is an acidic, refreshing, and non-alcoholic traditional Mayan beverage made with nixtamalized corn dough that is fermented spontaneously. The extensive analysis of the microbiology, biochemistry and metaproteomics of pozol allowed the construction of a comprehensive image of the fermentation system. The main changes in both the substrate and the microbiota occurred in the first 9 h of fermentation. The increase in microorganisms correlated with the drop in pH and with the decrease in the contents of carbohydrates, lipids, and nitrogen, which shows that this stage has the highest metabolic activity. Bacterial proteins were mainly represented by those of lactic acid bacteria, and among them, the proteins from genus
    Language English
    Publishing date 2021-08-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2776676-7
    ISSN 2296-861X
    ISSN 2296-861X
    DOI 10.3389/fnut.2021.714814
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  5. Article: Impact of c-di-GMP on the Extracellular Proteome of

    Lorite, María J / Casas-Román, Ariana / Girard, Lourdes / Encarnación, Sergio / Díaz-Garrido, Natalia / Badía, Josefa / Baldomá, Laura / Pérez-Mendoza, Daniel / Sanjuán, Juan

    Biology

    2022  Volume 12, Issue 1

    Abstract: Extracellular matrix components of bacterial biofilms include biopolymers such as polysaccharides, nucleic acids and proteins. Similar to polysaccharides, the secretion of adhesins and other matrix proteins can be regulated by the second messenger cyclic ...

    Abstract Extracellular matrix components of bacterial biofilms include biopolymers such as polysaccharides, nucleic acids and proteins. Similar to polysaccharides, the secretion of adhesins and other matrix proteins can be regulated by the second messenger cyclic diguanylate (cdG). We have performed quantitative proteomics to determine the extracellular protein contents of a
    Language English
    Publishing date 2022-12-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology12010044
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  6. Article ; Online: The human bile salt sodium deoxycholate induces metabolic and cell envelope changes in

    Olivar-Casique, Isaac B / Medina-Aparicio, Liliana / Mayo, Selena / Gama-Martínez, Yitzel / Rebollar-Flores, Javier E / Martínez-Batallar, Gabriel / Encarnación, Sergio / Calva, Edmundo / Hernández-Lucas, Ismael

    Journal of medical microbiology

    2022  Volume 71, Issue 1

    Abstract: Introduction. ...

    Abstract Introduction.
    MeSH term(s) Bile ; Bile Acids and Salts/chemistry ; Deoxycholic Acid/chemistry ; Humans ; Proteomics ; Salmonella typhi/metabolism ; Transcriptome
    Chemical Substances Bile Acids and Salts ; Deoxycholic Acid (005990WHZZ)
    Language English
    Publishing date 2022-01-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 218356-0
    ISSN 1473-5644 ; 0022-2615
    ISSN (online) 1473-5644
    ISSN 0022-2615
    DOI 10.1099/jmm.0.001461
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    In stock of ZB MED Cologne/Königswinter

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  7. Article ; Online: Nitrogen Fixation in Pozol, a Traditional Fermented Beverage.

    Rizo, Jocelin / Rogel, Marco A / Guillén, Daniel / Wacher, Carmen / Martinez-Romero, Esperanza / Encarnación, Sergio / Sánchez, Sergio / Rodríguez-Sanoja, Romina

    Applied and environmental microbiology

    2020  Volume 86, Issue 16

    Abstract: Traditional fermentations have been widely studied from the microbiological point of view, but little is known from the functional perspective. In this work, nitrogen fixation by free-living nitrogen-fixing bacteria was conclusively demonstrated in pozol, ...

    Abstract Traditional fermentations have been widely studied from the microbiological point of view, but little is known from the functional perspective. In this work, nitrogen fixation by free-living nitrogen-fixing bacteria was conclusively demonstrated in pozol, a traditional Mayan beverage prepared with nixtamalized and fermented maize dough. Three aspects of nitrogen fixation were investigated to ensure that fixation actually happens in the dough: (i) the detection of acetylene reduction activity directly in the substrate, (ii) the presence of potential diazotrophs, and (iii) an
    MeSH term(s) Acetylene/metabolism ; Bacteria/metabolism ; Enterobacter/isolation & purification ; Enterobacter/metabolism ; Enterobacteriaceae/isolation & purification ; Enterobacteriaceae/metabolism ; Fermented Foods and Beverages/microbiology ; Klebsiella/isolation & purification ; Klebsiella/metabolism ; Mexico ; Nitrogen Fixation ; Oxidation-Reduction ; Oxidoreductases/analysis ; RNA, Bacterial/analysis ; RNA, Ribosomal, 16S/analysis
    Chemical Substances RNA, Bacterial ; RNA, Ribosomal, 16S ; Oxidoreductases (EC 1.-) ; nitrogenase reductase (EC 1.18.6.1) ; Acetylene (OC7TV75O83)
    Language English
    Publishing date 2020-08-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.00588-20
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  8. Article: Qualitative changes in proteins contained in outer membrane vesicles produced by Rhizobium etli grown in the presence of the nod gene inducer naringenin

    Taboada, Hermenegildo / Andrade-Domínguez, Andrés / Buchs, Natasha / Dunn, Michael F / Encarnación, Sergio / Meneses, Niurka / Vargas-Lagunas, Carmen

    Archives of microbiology. 2019 Nov., v. 201, no. 9

    2019  

    Abstract: In this work, we compared the proteomic profiles of outer membrane vesicles (OMVs) isolated from Rhizobium etli CE3 grown in minimal medium (MM) with and without exogenous naringenin. One-hundred and seven proteins were present only in OMVs from ... ...

    Abstract In this work, we compared the proteomic profiles of outer membrane vesicles (OMVs) isolated from Rhizobium etli CE3 grown in minimal medium (MM) with and without exogenous naringenin. One-hundred and seven proteins were present only in OMVs from naringenin-containing cultures (N-OMVs), 57 proteins were unique to OMVs from control cultures lacking naringenin (C-OMVs) and 303 proteins were present in OMVs from both culture conditions (S-OMVs). Although we found no absolute predominance of specific types of proteins in the N-, C- or S-OMV classes, there were categories of proteins that were significantly less or more common in the different OMV categories. Proteins for energy production, translation and membrane and cell wall biogenesis were overrepresented in C-OMVs relative to N-OMVs. Proteins for carbohydrate metabolism and transport and those classified as either general function prediction only, function unknown, or without functional prediction were more common in N-OMVs than C-OMVs. This indicates that naringenin increased the proportion of these proteins in the OMVs, although NodD binding sites were only slightly more common in the promoters of genes for proteins found in the N-OMVs. In addition, OMVs from naringenin-containing cultures contained nodulation factor.
    Keywords binding sites ; biogenesis ; carbohydrate metabolism ; cell walls ; energy ; genes ; naringenin ; nodulation ; prediction ; proteins ; proteomics ; Rhizobium etli
    Language English
    Dates of publication 2019-11
    Size p. 1173-1194.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 124824-8
    ISSN 1432-072X ; 0302-8933
    ISSN (online) 1432-072X
    ISSN 0302-8933
    DOI 10.1007/s00203-019-01682-4
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  9. Article ; Online: The space of enzyme regulation in HeLa cells can be inferred from its intracellular metabolome.

    Diener, Christian / Muñoz-Gonzalez, Felipe / Encarnación, Sergio / Resendis-Antonio, Osbaldo

    Scientific reports

    2016  Volume 6, Page(s) 28415

    Abstract: During the transition from a healthy state to a cancerous one, cells alter their metabolism to increase proliferation. The underlying metabolic alterations may be caused by a variety of different regulatory events on the transcriptional or post- ... ...

    Abstract During the transition from a healthy state to a cancerous one, cells alter their metabolism to increase proliferation. The underlying metabolic alterations may be caused by a variety of different regulatory events on the transcriptional or post-transcriptional level whose identification contributes to the rational design of therapeutic targets. We present a mechanistic strategy capable of inferring enzymatic regulation from intracellular metabolome measurements that is independent of the actual mechanism of regulation. Here, enzyme activities are expressed by the space of all feasible kinetic constants (k-cone) such that the alteration between two phenotypes is given by their corresponding kinetic spaces. Deriving an expression for the transformation of the healthy to the cancer k-cone we identified putative regulated enzymes between the HeLa and HaCaT cell lines. We show that only a few enzymatic activities change between those two cell lines and that this regulation does not depend on gene transcription but is instead post-transcriptional. Here, we identify phosphofructokinase as the major driver of proliferation in HeLa cells and suggest an optional regulatory program, associated with oxidative stress, that affects the activity of the pentose phosphate pathway.
    Language English
    Publishing date 2016-06-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/srep28415
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  10. Article: The CRISPR-Cas System Is Involved in OmpR Genetic Regulation for Outer Membrane Protein Synthesis in

    Medina-Aparicio, Liliana / Rodriguez-Gutierrez, Sarahí / Rebollar-Flores, Javier E / Martínez-Batallar, Ángel G / Mendoza-Mejía, Blanca D / Aguirre-Partida, Eira D / Vázquez, Alejandra / Encarnación, Sergio / Calva, Edmundo / Hernández-Lucas, Ismael

    Frontiers in microbiology

    2021  Volume 12, Page(s) 657404

    Abstract: The CRISPR-Cas cluster is found in many prokaryotic genomes including those of the Enterobacteriaceae family. ...

    Abstract The CRISPR-Cas cluster is found in many prokaryotic genomes including those of the Enterobacteriaceae family.
    Language English
    Publishing date 2021-03-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2021.657404
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