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  1. Article ; Online: Using Microfluidics to Model Mucus.

    Engevik, Amy C

    Cellular and molecular gastroenterology and hepatology

    2020  Volume 9, Issue 3, Page(s) 551–552

    MeSH term(s) Humans ; Irritable Bowel Syndrome ; Lab-On-A-Chip Devices ; Microfluidics ; Mucus
    Language English
    Publishing date 2020-01-06
    Publishing country United States
    Document type Editorial ; Comment
    ISSN 2352-345X
    ISSN (online) 2352-345X
    DOI 10.1016/j.jcmgh.2019.12.003
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  2. Article ; Online: Myosins and membrane trafficking in intestinal brush border assembly.

    Engevik, Melinda A / Engevik, Amy C

    Current opinion in cell biology

    2022  Volume 77, Page(s) 102117

    Abstract: Myosins are a class of motors that participate in a wide variety of cellular functions including organelle transport, cell adhesion, endocytosis and exocytosis, movement of RNA, and cell motility. Among the emerging roles for myosins is regulation of the ...

    Abstract Myosins are a class of motors that participate in a wide variety of cellular functions including organelle transport, cell adhesion, endocytosis and exocytosis, movement of RNA, and cell motility. Among the emerging roles for myosins is regulation of the assembly, morphology, and function of actin protrusions such as microvilli. The intestine harbors an elaborate apical membrane composed of highly organized microvilli. Microvilli assembly and function are intricately tied to several myosins including Myosin 1a, non-muscle Myosin 2c, Myosin 5b, Myosin 6, and Myosin 7b. Here, we review the research progress made in our understanding of myosin mediated apical assembly.
    MeSH term(s) Actins/metabolism ; Cell Membrane/metabolism ; Intestines ; Microvilli/metabolism ; Myosins/metabolism
    Chemical Substances Actins ; Myosins (EC 3.6.4.1)
    Language English
    Publishing date 2022-07-20
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1026381-0
    ISSN 1879-0410 ; 0955-0674
    ISSN (online) 1879-0410
    ISSN 0955-0674
    DOI 10.1016/j.ceb.2022.102117
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  3. Article ; Online: Bioinformatics reveal elevated levels of Myosin Vb in uterine corpus endometrial carcinoma patients which correlates to increased cell metabolism and poor prognosis.

    Engevik, Kristen A / Engevik, Melinda A / Engevik, Amy C

    PloS one

    2023  Volume 18, Issue 1, Page(s) e0280428

    Abstract: Carcinoma of the endometrium of the uterus is the most common female pelvic malignancy. Although uterine corpus endometrial cancer (UCEC) has a favorable prognosis if removed early, patients with advanced tumor stages have a low survival rate. These ... ...

    Abstract Carcinoma of the endometrium of the uterus is the most common female pelvic malignancy. Although uterine corpus endometrial cancer (UCEC) has a favorable prognosis if removed early, patients with advanced tumor stages have a low survival rate. These facts highlight the importance of understanding UCEC biology. Computational analysis of RNA-sequencing data from UCEC patients revealed that the molecular motor Myosin Vb (MYO5B) was elevated in the beginning stages of UCEC and occurred in all patients regardless of tumor stage, tumor type, age, menopause status or ethnicity. Although several mutations were identified in the MYO5B gene in UCEC patients, these mutations did not correlate with mRNA expression. Examination of MYO5B methylation revealed that UCEC patients had undermethylated MYO5B and undermethylation was positively correlated with increased mRNA and protein levels. Immunostaining confirmed elevated levels of apical MYO5B in UCEC patients compared to adjacent tissue. UCEC patients with high expressing MYO5B tumors had far worse prognosis than UCEC patients with low expressing MYO5B tumors, as reflected by survival curves. Metabolic pathway analysis revealed significant alterations in metabolism pathways in UCE patients and key metabolism genes were positively correlated with MYO5B mRNA. These data provide the first evidence that MYO5B may participate in UCEC tumor development.
    MeSH term(s) Humans ; Female ; Carcinoma, Endometrioid ; Endometrial Neoplasms/pathology ; Prognosis ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Computational Biology ; Myosins
    Chemical Substances RNA, Messenger ; Myosins (EC 3.6.4.1)
    Language English
    Publishing date 2023-01-20
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0280428
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  4. Article ; Online: Identification of Differentiated Intestinal Epithelial Cells Using Immunostaining and Fluorescence Microscopy.

    Digrazia, Jessica R / Engevik, Melinda A / Engevik, Amy C

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2650, Page(s) 17–34

    Abstract: Immunofluorescence imaging enables visualization of a wide range of molecules in diverse cells and tissues. Determining the localization and endogenous protein levels in cells using immunostaining can be highly informative for researchers studying cell ... ...

    Abstract Immunofluorescence imaging enables visualization of a wide range of molecules in diverse cells and tissues. Determining the localization and endogenous protein levels in cells using immunostaining can be highly informative for researchers studying cell structure and function. The small intestinal epithelium is composed of numerous cell types including absorptive enterocytes, mucus-producing goblet cells, lysozyme positive Paneth cells, proliferative stem cells, chemosensing tuft cells, and hormone-producing enteroendocrine cells. Each cell type in the small intestine has unique functions and structures that are critical for maintaining intestinal homeostasis and identifiable by immunofluorescence labeling. In this chapter we provide a detailed protocol and representative images of immunostaining of paraffin-embedded mouse small intestinal tissue. The method highlights antibodies and micrographs that identify differentiated cell types. These details are important because quality immunofluorescence imaging can provide novel insights and a greater understanding of healthy and disease states.
    MeSH term(s) Animals ; Mice ; Epithelial Cells ; Intestines ; Cell Differentiation ; Enteroendocrine Cells ; Microscopy, Fluorescence
    Language English
    Publishing date 2023-07-17
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3076-1_2
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  5. Article ; Online: The Age of Angiogenesis: A Novel Role of NGF in Gastric Repair.

    Engevik, Amy C

    Cellular and molecular gastroenterology and hepatology

    2018  Volume 6, Issue 2, Page(s) 227–228

    Language English
    Publishing date 2018-06-28
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 2819778-1
    ISSN 2352-345X ; 2352-345X
    ISSN (online) 2352-345X
    ISSN 2352-345X
    DOI 10.1016/j.jcmgh.2018.06.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Exploring the impact of intestinal ion transport on the gut microbiota.

    Engevik, Amy C / Engevik, Melinda A

    Computational and structural biotechnology journal

    2020  Volume 19, Page(s) 134–144

    Abstract: The gut microbiota and the host are intimately connected. The host physiology dictates the intestinal environment through regulation of pH, ion concentration, mucus production, ...

    Abstract The gut microbiota and the host are intimately connected. The host physiology dictates the intestinal environment through regulation of pH, ion concentration, mucus production,
    Language English
    Publishing date 2020-12-16
    Publishing country Netherlands
    Document type Journal Article ; Review
    ISSN 2001-0370
    ISSN 2001-0370
    DOI 10.1016/j.csbj.2020.12.008
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  7. Article ; Online: Modeling Microvillus Inclusion Formation In Vitro.

    Engevik, Amy C / Goldenring, James R

    Cellular and molecular gastroenterology and hepatology

    2018  Volume 6, Issue 4, Page(s) 472–473

    MeSH term(s) Enterocytes ; Humans ; Inclusion Bodies ; Microvilli ; Mucolipidoses ; Myosin Type V
    Chemical Substances Myosin Type V (EC 3.6.1.-)
    Language English
    Publishing date 2018-09-05
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 2819778-1
    ISSN 2352-345X ; 2352-345X
    ISSN (online) 2352-345X
    ISSN 2352-345X
    DOI 10.1016/j.jcmgh.2018.08.002
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  8. Article: Acinetobacter calcoaceticus

    Glover, Janiece S / Browning, Brittney D / Ticer, Taylor D / Engevik, Amy C / Engevik, Melinda A

    Frontiers in physiology

    2022  Volume 13, Page(s) 880024

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2022-05-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2022.880024
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  9. Article ; Online: The Physiology of the Gastric Parietal Cell.

    Engevik, Amy C / Kaji, Izumi / Goldenring, James R

    Physiological reviews

    2019  Volume 100, Issue 2, Page(s) 573–602

    Abstract: Parietal cells are responsible for gastric acid secretion, which aids in the digestion of food, absorption of minerals, and control of harmful bacteria. However, a fine balance of activators and inhibitors of parietal cell-mediated acid secretion is ... ...

    Abstract Parietal cells are responsible for gastric acid secretion, which aids in the digestion of food, absorption of minerals, and control of harmful bacteria. However, a fine balance of activators and inhibitors of parietal cell-mediated acid secretion is required to ensure proper digestion of food, while preventing damage to the gastric and duodenal mucosa. As a result, parietal cell secretion is highly regulated through numerous mechanisms including the vagus nerve, gastrin, histamine, ghrelin, somatostatin, glucagon-like peptide 1, and other agonists and antagonists. The tight regulation of parietal cells ensures the proper secretion of HCl. The H
    MeSH term(s) Animals ; Cell Shape ; Gastric Acid/metabolism ; H(+)-K(+)-Exchanging ATPase/metabolism ; Homeostasis ; Humans ; Parietal Cells, Gastric/drug effects ; Parietal Cells, Gastric/metabolism ; Potassium/metabolism ; Proton Pump Inhibitors/pharmacology ; Secretory Pathway ; Signal Transduction
    Chemical Substances Proton Pump Inhibitors ; H(+)-K(+)-Exchanging ATPase (EC 3.6.3.10) ; Potassium (RWP5GA015D)
    Keywords covid19
    Language English
    Publishing date 2019-10-31
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 209902-0
    ISSN 1522-1210 ; 0031-9333
    ISSN (online) 1522-1210
    ISSN 0031-9333
    DOI 10.1152/physrev.00016.2019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Uc I Zs.166: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  10. Article ; Online: Chief cell plasticity is the origin of metaplasia following acute injury in the stomach mucosa.

    Caldwell, Brianna / Meyer, Anne R / Weis, Jared A / Engevik, Amy C / Choi, Eunyoung

    Gut

    2021  Volume 71, Issue 6, Page(s) 1068–1077

    Abstract: Objective: Metaplasia arises from differentiated cell types in response to injury and is considered a precursor in many cancers. Heterogeneous cell lineages are present in the reparative metaplastic mucosa with response to injury, including foveolar ... ...

    Abstract Objective: Metaplasia arises from differentiated cell types in response to injury and is considered a precursor in many cancers. Heterogeneous cell lineages are present in the reparative metaplastic mucosa with response to injury, including foveolar cells, proliferating cells and spasmolytic polypeptide-expressing metaplasia (SPEM) cells, a key metaplastic cell population. Zymogen-secreting chief cells are long-lived cells in the stomach mucosa and have been considered the origin of SPEM cells; however, a conflicting paradigm has proposed isthmal progenitor cells as an origin for SPEM.
    Design: Gastric intrinsic factor (GIF) is a stomach tissue-specific gene and exhibits protein expression unique to mature mouse chief cells. We generated a novel chief cell-specific driver mouse allele, GIF-rtTA. GIF-GFP reporter mice were used to validate specificity of GIF-rtTA driver in chief cells. GIF-Cre-RnTnG mice were used to perform lineage tracing during homoeostasis and acute metaplasia development. L635 treatment was used to induce acute mucosal injury and coimmunofluorescence staining was performed for various gastric lineage markers.
    Results: We demonstrated that mature chief cells, rather than isthmal progenitor cells, serve as the predominant origin of SPEM cells during the metaplastic process after acute mucosal injury. Furthermore, we observed long-term label-retaining chief cells at 1 year after the GFP labelling in chief cells. However, only a very small subset of the long-term label-retaining chief cells displayed the reprogramming ability in homoeostasis. In contrast, we identified chief cell-originating SPEM cells as contributing to lineages within foveolar cell hyperplasia in response to the acute mucosal injury.
    Conclusion: Our study provides pivotal evidence for cell plasticity and lineage contributions from differentiated gastric chief cells during acute metaplasia development.
    MeSH term(s) Animals ; Cell Plasticity ; Chief Cells, Gastric/metabolism ; Gastric Mucosa/metabolism ; Humans ; Metaplasia/metabolism ; Mice ; Stomach ; Stomach Neoplasms/metabolism
    Language English
    Publishing date 2021-09-08
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 80128-8
    ISSN 1468-3288 ; 0017-5749
    ISSN (online) 1468-3288
    ISSN 0017-5749
    DOI 10.1136/gutjnl-2021-325310
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