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Article: Diagnostic kit based on halloysite nanoclay-ionic liquid nanocomposite modified electrode for electrochemical determination of cancer biomarker

Yildiz, Esma / Yurdacan, Beste / Erac, Yasemin / Erdem, Arzum

Talanta. 2023 Jan. 15, v. 252

2023

Abstract: Nucleic acid hybridization is occurred between the selective single-stranded nucleic acid sequence and its target sequence, which is one of the essential procedure for electrochemical detection of nucleic acid. microRNA-21 (miRNA-21) is known as a ... ...

Abstract	Nucleic acid hybridization is occurred between the selective single-stranded nucleic acid sequence and its target sequence, which is one of the essential procedure for electrochemical detection of nucleic acid. microRNA-21 (miRNA-21) is known as a biomarker in various cancers. The determination of miRNA-21 was attained through by hybridization of inosine substituted miRNA-21 specific DNA probe (Pᵢₙₒₛᵢₙₑ) with its target miRNA-21. In this study, the surface of pencil graphite electrode (PGE) was firstly modified with halloysite nanoclay-ionic liquid (HNT/IL) nanocomposite. The characterization of surface was performed by Scanning Electron Microscope (SEM) images and Energy Dispersive X-Ray Analysis (EDX) analysis, and the differences at surface modifications were also shown by electrochemical methods with electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). For sensitive and selective determination of miRNA-21, Pᵢₙₒₛᵢₙₑ and target miRNA concentration, immobilization and hybridization time were optimized by using HNT/IL modified PGE in combination with differential pulse voltammetry (DPV). The detection limit was achieved as 0.17 μg/mL (equals to 23.69 nM) in the linear range of 0.25–2 μg/mL miRNA-21. The selectivity of voltammetric method based on HNT/IL-PGE developed for miRNA-21 was examined in the presence of mismatch (MM) and non-complementary (NC) sequences. Because miRNA-21 is over-expressed in cancer cells, it has been tested in total RNA samples isolated from cancer cell line (breast cancer cell line, MCF-7). In the total RNA samples obtained from MCF-7, the detection limit was calculated as 0.28 μg/mL in the linear range of 1–4 μg/mL. Besides, the healthy cell line (human embryonic kidney cell line, HEK-293) was used as a control group and the results obtained by MCF-7 total RNA samples were compared to the results using HEK-293 total RNA samples in terms of miRNA-21 level.
Keywords	DNA probes ; RNA ; analytical kits ; biomarkers ; breast neoplasms ; carbon electrodes ; cell lines ; detection limit ; dielectric spectroscopy ; electrochemistry ; energy-dispersive X-ray analysis ; halloysite ; humans ; hybridization ; inosine ; kidneys ; liquids ; nanocomposites ; neoplasm cells ; nucleic acid hybridization ; nucleotide sequences ; voltammetry
Language	English
Dates of publication	2023-0115
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2022.123854
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Diagnostic kit based on halloysite nanoclay-ionic liquid nanocomposite modified electrode for electrochemical determination of cancer biomarker.

Yildiz, Esma / Yurdacan, Beste / Erac, Yasemin / Erdem, Arzum

Talanta

2022 Volume 252, Page(s) 123854

Abstract	Nucleic acid hybridization is occurred between the selective single-stranded nucleic acid sequence and its target sequence, which is one of the essential procedure for electrochemical detection of nucleic acid. microRNA-21 (miRNA-21) is known as a biomarker in various cancers. The determination of miRNA-21 was attained through by hybridization of inosine substituted miRNA-21 specific DNA probe (P
MeSH term(s)	Humans ; Ionic Liquids ; Clay ; Biomarkers, Tumor/genetics ; Biosensing Techniques/methods ; HEK293 Cells ; Electrodes ; Electrochemical Techniques/methods ; Graphite/chemistry ; DNA Probes/chemistry ; MicroRNAs/analysis ; Nanocomposites ; Inosine ; Prostaglandins E ; Neoplasms/diagnosis
Chemical Substances	Ionic Liquids ; Clay (T1FAD4SS2M) ; Biomarkers, Tumor ; Graphite (7782-42-5) ; DNA Probes ; MicroRNAs ; Inosine (5A614L51CT) ; Prostaglandins E
Language	English
Publishing date	2022-08-19
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2022.123854
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Article: Electrochemical Investigation of Curcumin-DNA Interaction by Using Hydroxyapatite Nanoparticles-Ionic Liquids Based Composite Electrodes.

Uca, Merve / Eksin, Ece / Erac, Yasemin / Erdem, Arzum

Materials (Basel, Switzerland)

2021 Volume 14, Issue 15

Abstract: Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy ( ...

Abstract	Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applied to examine the microscopic and electrochemical characterization of HaP and IL-modified biosensors. The interaction of curcumin with nucleic acids and polymerase chain reaction (PCR) samples was investigated by measuring the changes at the oxidation signals of both curcumin and guanine by differential pulse voltammetry (DPV) technique. The optimization of curcumin concentration, DNA concentration, and the interaction time was performed. The interaction of curcumin with PCR samples was also investigated by gel electrophoresis.
Language	English
Publishing date	2021-08-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma14154344
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Article ; Online: The inhibitory effect of escitalopram on mouse detrusor contractility: The role of L-type calcium channels.

Engin, Seçkin / Barut, Elif Nur / Erac, Yasemin / Sari, Suat / Kadioglu, Mine

Toxicology and applied pharmacology

2023 Volume 461, Page(s) 116408

Abstract: Selective serotonin reuptake inhibitors (SSRIs) are associated with urinary problems attributed to their central effects. ESC is a preferred SSRI and several case reports described that ESC is related to urinary retention. However, the direct effect of ... ...

Abstract	Selective serotonin reuptake inhibitors (SSRIs) are associated with urinary problems attributed to their central effects. ESC is a preferred SSRI and several case reports described that ESC is related to urinary retention. However, the direct effect of ESC on detrusor contractility is still not completely elucidated. Thus, we investigated the effect of ESC on detrusor contractility and mechanism(s) of its action in isolated mouse detrusor strips. Molecular docking and measurement of intracellular calcium were performed to determine the possible calcium channel blocking effect of ESC. The contractile responses to carbachol (CCh), KCl and electrical field stimulation of detrusor strips were significantly abolished by ESC (10 or 100 μM). ESC relaxed KCl-precontracted detrusor strips concentration-dependently, which was not affected by tetraethylammonium, glibenclamide, 4-aminopyridine, propranolol, L-NAME or methylene blue. ESC (10 or 100 μM) reduced both the CaCl
MeSH term(s)	Mice ; Animals ; Calcium Channels, L-Type ; Urinary Bladder ; Escitalopram ; Molecular Docking Simulation ; Carbachol/pharmacology ; Muscle Contraction
Chemical Substances	Calcium Channels, L-Type ; Escitalopram (4O4S742ANY) ; Carbachol (8Y164V895Y)
Language	English
Publishing date	2023-02-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	204477-8
ISSN	1096-0333 ; 0041-008X
ISSN (online)	1096-0333
ISSN	0041-008X
DOI	10.1016/j.taap.2023.116408
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Zs.B 14: Show issues

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Article ; Online: Nonsteroidal antiinflammatory drugs alter antibiotic susceptibility and expression of virulence-related genes and protein A of Staphylococcus aureus

Öztürk, İsmail / Eraç, Yasemin / Ballar Kırmızibayrak, Petek / Ermertcan, Şafak

Turkish journal of medical sciences

2021 Volume 51, Issue 2, Page(s) 835–847

Abstract: Background/aim: Nonsteroidal antiinflammatory drugs (NSAIDs) including diclofenac, naproxen, ibuprofen, acetylsalicylic acid, and acetaminophen have been shown to have antimicrobial effects on various microorganisms. The aim of this study was to ... ...

Abstract	Background/aim: Nonsteroidal antiinflammatory drugs (NSAIDs) including diclofenac, naproxen, ibuprofen, acetylsalicylic acid, and acetaminophen have been shown to have antimicrobial effects on various microorganisms. The aim of this study was to investigate the antibacterial effects of NSAIDs on Staphylococcus aureus. Materials and methods: Susceptibilities of S. aureus strains to NSAIDs with or without antimicrobials (moxifloxacin, vancomycin, ciprofloxacin, clindamycin, and gentamicin) were determined using the microdilution method and disk diffusion test. Expression levels of genes in the presence of drugs were investigated by real-time quantitative RT-PCR (qRT-PCR), and immunoblotting analysis was performed for staphylococcal protein A (SpA). Results: Our results showed that all NSAIDs were active against S. aureus strains with MIC values ranging from 195 μg/mL to 6250 μg/ mL. NSAIDs increased the antibiotic susceptibility of the strains, and diclofenac was found to be more effective than the other drugs. Drugs showed different effects on expression levels of virulence factor and/or regulatory genes. Immunoblotting analysis of SpA protein was mostly in accordance with qRT-PCR results. Conclusion: The regulatory/virulence factor genes and proteins of S. aureus investigated in this study may be reasonable targets for these drugs, and we suggest that the data may contribute to the field of infection control and antimicrobial resistance.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Diclofenac/pharmacology ; Gene Expression/drug effects ; Humans ; Microbial Sensitivity Tests ; Real-Time Polymerase Chain Reaction ; Staphylococcal Infections/drug therapy ; Staphylococcal Protein A/genetics ; Staphylococcal Protein A/therapeutic use ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/genetics ; Virulence/drug effects ; Virulence/genetics ; Virulence Factors/genetics
Chemical Substances	Anti-Bacterial Agents ; Anti-Inflammatory Agents, Non-Steroidal ; Staphylococcal Protein A ; Virulence Factors ; Diclofenac (144O8QL0L1)
Language	English
Publishing date	2021-04-30
Publishing country	Turkey
Document type	Journal Article
ZDB-ID	1183461-4
ISSN	1303-6165 ; 1300-0144
ISSN (online)	1303-6165
ISSN	1300-0144
DOI	10.3906/sag-2003-60
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Zs.A 3242: Show issues

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Article ; Online: The inhibitory effect of trimetazidine on detrusor contractility - a potential repositioning of trimetazidine for the treatment of overactive bladder.

Engin, Seckin / Kaya Yasar, Yesim / Barut, Elif Nur / Getboga, Damla / Erac, Yasemin / Sezen, Sena F

The Journal of pharmacy and pharmacology

2021 Volume 74, Issue 1, Page(s) 94–102

Abstract: Objectives: This study aimed to identify the effect of trimetazidine (TMZ), an antianginal drug, on detrusor smooth muscle (DSM) contractility and its possible mechanisms of action.: Methods: We performed in-vitro contractility studies on isolated ... ...

Abstract	Objectives: This study aimed to identify the effect of trimetazidine (TMZ), an antianginal drug, on detrusor smooth muscle (DSM) contractility and its possible mechanisms of action. Methods: We performed in-vitro contractility studies on isolated mouse DSM strips and investigated the effect of TMZ on Ca2+ levels in fura-2-loaded A7r5 cells. Key findings: TMZ (300 or 1000 µM) inhibited carbachol (CCh)- and KCl-induced contractions and produced a concentration-dependent (10-1000 µM) relaxation in KCl-precontracted DSM strips. TMZ-induced relaxation was markedly decreased by BaCl2, an inward-rectifying K+ channel blocker, but was not altered by preincubation with tetraethylammonium, glibenclamide, 4-aminopyridine, propranolol, L-NAME or methylene blue. TMZ (300 or 1000 µM) reduced both the CaCl2-induced contraction of depolarized DSM strips under Ca2+-free conditions and the CCh-induced contraction of DSM strips preincubated with nifedipine in Ca2+-containing Krebs solution. Furthermore, TMZ (1000 µM) significantly decreased the Ca2+ levels in fura-2-loaded A7r5 cells. Conclusions: TMZ decreased DSM contractility and caused a concentration-dependent relaxation of the tissue possibly through its actions on Ca2+ transients and K+ channels. Our results provide preclinical evidence that TMZ would be a potential candidate to treat disorders related to the overactivity of the bladder.
MeSH term(s)	Animals ; Calcium Channel Blockers/pharmacology ; Calcium Channels, T-Type/metabolism ; Drug Repositioning/methods ; Ion Channels/metabolism ; Mice ; Muscle Contraction/drug effects ; Muscle Contraction/physiology ; Muscle, Smooth/drug effects ; Nifedipine/pharmacology ; Trimetazidine/pharmacology ; Urinary Bladder/drug effects ; Urinary Bladder/pathology ; Urinary Bladder/physiopathology ; Urinary Bladder, Overactive/drug therapy ; Urinary Bladder, Overactive/physiopathology ; Vasodilator Agents/pharmacology
Chemical Substances	Calcium Channel Blockers ; Calcium Channels, T-Type ; Ion Channels ; Vasodilator Agents ; Nifedipine (I9ZF7L6G2L) ; Trimetazidine (N9A0A0R9S8)
Language	English
Publishing date	2021-06-10
Publishing country	England
Document type	Journal Article
ZDB-ID	3107-0
ISSN	2042-7158 ; 0022-3573 ; 0373-1022
ISSN (online)	2042-7158
ISSN	0022-3573 ; 0373-1022
DOI	10.1093/jpp/rgab072
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Uf I Zs.149: Show issues

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Article: Implications of Possible HBV-Driven Regulation of Gene Expression in Stem Cell-like Subpopulation of Huh-7 Hepatocellular Carcinoma Cell Line.

Demir, Ayse Banu / Benvenuto, Domenico / Karacicek, Bilge / Erac, Yasemin / Spoto, Silvia / Angeletti, Silvia / Ciccozzi, Massimo / Tosun, Metiner

Journal of personalized medicine

2022 Volume 12, Issue 12

Abstract: Elevated levels of STIM1, an endoplasmic reticulum ... ...

Abstract	Elevated levels of STIM1, an endoplasmic reticulum Ca
Language	English
Publishing date	2022-12-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662248-8
ISSN	2075-4426
ISSN	2075-4426
DOI	10.3390/jpm12122065
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Article ; Online: Mechanism of adrenomedullin 2/intermedin mediated vasorelaxation in rat main pulmonary artery.

Telli, Gokcen / Erac, Yasemin / Tel, Banu Cahide / Gumusel, Bulent

Peptides

2018 Volume 103, Page(s) 65–71

Abstract: Adrenomedullin 2/intermedin (AM2/IMD) is a member of calcitonin related gene peptide family and an important nitric oxide mediated vasorelaxant in various vascular beds. However, the mechanism of post receptor-interaction is not clear and may differ ... ...

Abstract	Adrenomedullin 2/intermedin (AM2/IMD) is a member of calcitonin related gene peptide family and an important nitric oxide mediated vasorelaxant in various vascular beds. However, the mechanism of post receptor-interaction is not clear and may differ depending on tissue type and species. In this study, we aimed to investigate the exact mechanism and the role of BK
MeSH term(s)	Adrenomedullin/antagonists & inhibitors ; Adrenomedullin/metabolism ; Animals ; Apamin/pharmacology ; Calcium/metabolism ; Glyburide/pharmacology ; Neuropeptides/antagonists & inhibitors ; Neuropeptides/metabolism ; Peptides/pharmacology ; Pulmonary Artery/drug effects ; Rats ; Vasodilation/drug effects ; Verapamil/pharmacology
Chemical Substances	Adm2 protein, rat ; Neuropeptides ; Peptides ; Adrenomedullin (148498-78-6) ; Apamin (24345-16-2) ; iberiotoxin (773HER9B6T) ; Verapamil (CJ0O37KU29) ; Glyburide (SX6K58TVWC) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2018-03-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	769028-9
ISSN	1873-5169 ; 0196-9781
ISSN (online)	1873-5169
ISSN	0196-9781
DOI	10.1016/j.peptides.2018.03.015
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Zs.A 1649: Show issues

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Article ; Online: Silencing acpP gene via antisense oligonucleotide-niosome complex in clinical Pseudomonas aeruginosa isolates.

Tekintas, Yamac / Demir-Dora, Devrim / Erac, Bayrı / Erac, Yasemin / Yilmaz, Ozlem / Aydemir, Sabire Sohret / Kocagoz, Zuhtu Tanil / Hosgor-Limoncu, Mine

Research in microbiology

2021 Volume 172, Issue 4-5, Page(s) 103834

Abstract: Pseudomonas aeruginosa, an opportunistic Gram-negative pathogen, is one of the major causes of nosocomial infections. In addition to its physiological adaptation capacity, it can develop resistance to disinfectants and antibiotics through various ... ...

Abstract	Pseudomonas aeruginosa, an opportunistic Gram-negative pathogen, is one of the major causes of nosocomial infections. In addition to its physiological adaptation capacity, it can develop resistance to disinfectants and antibiotics through various mechanisms. Recently, new eradication methods are gaining attention. Therefore, in this study, an LNA-2'-O-methyl hybrid antisense oligonucleotide targeting the acyl carrier protein P (acpP) gene was introduced into P. aeruginosa isolates. The design was determined through sequence analysis and prediction of the secondary structure of mRNA by software. Niosomes were used for enhancing cellular uptake. The control of the binding and transfection ability of the sequence was determined fluorometrically by labeling with 6-Fam. The effects were determined with broth microdilution method and qPCR studies. Eight different formulations were prepared. Among these, one formulation has shown to have ASO complexation ability whose composition was 312 μl Span 80 + 69.5 mg Cholesterol+ 36.4 mg CTAB+1 ml Chloroform and 5 ml dH
MeSH term(s)	Acyl Carrier Protein/antagonists & inhibitors ; Anti-Bacterial Agents/pharmacology ; Biofilms/growth & development ; Drug Delivery Systems ; Gene Silencing ; Humans ; Liposomes/chemistry ; Liposomes/pharmacology ; Microbial Sensitivity Tests ; Oligonucleotides, Antisense/pharmacology ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/drug effects
Chemical Substances	Acyl Carrier Protein ; Anti-Bacterial Agents ; Liposomes ; Oligonucleotides, Antisense
Language	English
Publishing date	2021-04-21
Publishing country	France
Document type	Journal Article
ZDB-ID	1004220-9
ISSN	1769-7123 ; 0923-2508
ISSN (online)	1769-7123
ISSN	0923-2508
DOI	10.1016/j.resmic.2021.103834
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Zs.A 890: Show issues

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Article: Effects of cell seeding density on real-time monitoring of anti-proliferative effects of transient gene silencing.

Selli, Cigdem / Erac, Yasemin / Tosun, Metiner

Journal of biological research (Thessalonike, Greece)

2016 Volume 23, Page(s) 20

Abstract: Background: Real-time cellular analysis systems enable impedance-based label-free and dynamic monitoring of various cellular events such as proliferation. In this study, we describe the effects of initial cell seeding density on the anti-proliferative ... ...

Abstract	Background: Real-time cellular analysis systems enable impedance-based label-free and dynamic monitoring of various cellular events such as proliferation. In this study, we describe the effects of initial cell seeding density on the anti-proliferative effects of transient gene silencing monitored via real-time cellular analysis. We monitored the real-time changes in proliferation of Huh7 hepatocellular carcinoma and A7r5 vascular smooth muscle cells with different initial seeding densities following transient receptor potential canonical 1 (TRPC1) silencing using xCELLigence system. Huh7 and A7r5 cells were seeded on E-plate 96 at 10,000, 5000, 1250 and 5000, 2500 cells well Results: TRPC1 silencing did not inhibit the proliferation rates of Huh7 cells at 10,000 cells well Conclusions: Data suggest that the effects of transient silencing on cell proliferation differ depending on the initial cell seeding density. While high seeding densities mask the significant changes in proliferation, the inhibitory effects of silencing become apparent at lower seeding densities as the entry into log phase is delayed. Using the optimal initial seeding density is crucial when studying the effects of transient gene silencing. In addition, the results suggest that TRPC1 may contribute to proliferation and phenotypic switching of vascular smooth muscle cells.
Language	English
Publishing date	2016-12-01
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2186880-3
ISSN	2241-5793 ; 1790-045X
ISSN (online)	2241-5793
ISSN	1790-045X
DOI	10.1186/s40709-016-0057-4
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