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  1. AU="Erdmann, Niklas"
  2. AU="Kocataş, A (Kanuni Sultan Suleyman Training And Research Hospital)"

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  1. Artikel: Sporosarcina pasteurii

    Erdmann, Niklas / Kästner, Felix / de Payrebrune, Kristin / Strieth, Dorina

    Engineering in life sciences

    2022  Band 22, Heft 12, Seite(n) 760–768

    Abstract: When using microbiologically induced calcium carbonate precipitation (MICP) to produce calcium carbonate crystals in the cavities between mineral particles to consolidate them, the inhomogeneous distribution of the precipitated calcium carbonate poses a ... ...

    Abstract When using microbiologically induced calcium carbonate precipitation (MICP) to produce calcium carbonate crystals in the cavities between mineral particles to consolidate them, the inhomogeneous distribution of the precipitated calcium carbonate poses a problem for the production of construction materials with consistent parameters. Various approaches have been investigated in the literature to increase the homogeneity of consolidated samples. One approach can be the targeted application of ureolytic organisms by 3D printing. However, to date, this possibility has been little explored in the literature. In this study, the potential to use MICP to print calcium carbonate layers on mineral particles will be investigated. For this purpose, a dispensing unit was modified to apply both a suspension of
    Sprache Englisch
    Erscheinungsdatum 2022-06-16
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 2071199-2
    ISSN 1618-2863 ; 1618-0240
    ISSN (online) 1618-2863
    ISSN 1618-0240
    DOI 10.1002/elsc.202100074
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: New procedure for separation and analysis of the main components of cyanobacterial EPS.

    Strieth, Dorina / Schwarz, Anna / Stiefelmaier, Judith / Erdmann, Niklas / Muffler, Kai / Ulber, Roland

    Journal of biotechnology

    2021  Band 328, Seite(n) 78–86

    Abstract: Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer. EPS mainly consist of water, polysaccharides, proteins, lipids and ... ...

    Abstract Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer. EPS mainly consist of water, polysaccharides, proteins, lipids and nucleic acids as well as lysis and hydrolysis products which makes the composition very complex. Thus, rough simplifications are used and commonly one or at most two components of the EPS are examined. In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %. The method was established with synthetic EPS, which based on the composition of real EPS described in literature. Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample. The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days. It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture. While the amount of proteins in the EPS increased during the cultivation period, carbohydrates and lipids were dominant in the beginning and decreased afterwards.
    Mesh-Begriff(e) Biofilms ; Cyanobacteria ; Extracellular Polymeric Substance Matrix ; Polysaccharides ; Proteins
    Chemische Substanzen Polysaccharides ; Proteins
    Sprache Englisch
    Erscheinungsdatum 2021-01-20
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 843647-2
    ISSN 1873-4863 ; 0168-1656 ; 1389-0352
    ISSN (online) 1873-4863
    ISSN 0168-1656 ; 1389-0352
    DOI 10.1016/j.jbiotec.2021.01.007
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Characterization of terrestrial phototrophic biofilms of cyanobacterial species

    Stiefelmaier, Judith / Strieth, Dorina / Di Nonno, Sarah / Erdmann, Niklas / Muffler, Kai / Ulber, Roland

    Algal research. 2020 Sept., v. 50

    2020  

    Abstract: Terrestrial cyanobacteria are phototrophic microorganisms, which grow embedded in a matrix of extracellular polymeric substances (EPS) as biofilms. To perform photosynthesis, cyanobacteria have developed, additionally to chlorophyll-a and carotenoids, ... ...

    Abstract Terrestrial cyanobacteria are phototrophic microorganisms, which grow embedded in a matrix of extracellular polymeric substances (EPS) as biofilms. To perform photosynthesis, cyanobacteria have developed, additionally to chlorophyll-a and carotenoids, the so-called phycobilisomes, which consist of differently pigmented phycobiliproteins (PBPs). The production of natural dyes, pharmaceutical substances and their nutrient rich biomass makes cyanobacterial biofilms interesting research targets for industrial development. However, characterization of biofilms during cultivation implies several difficulties, since standard sampling applied in suspended cultures is not possible. The aim of this study was to find new possibilities for documenting biofilm growth. Therefore, two methods for non-invasive examination of phototrophic biofilms were combined. The terrestrial cyanobacteria Trichocoleus sociatus und Coleofasciculus chthonoplastes were cultivated surface-associated in an aerosol. Every three to four days biofilm thickness was measured using optical coherence tomography (OCT) and biofilm area using PAM fluorometry. Additionally, three replicates were harvested to determine biomass formation and pigment composition. According to our results, an increase of biomass, biofilm thickness and biofilm area was detected. The non-linear correlation between these parameters indicates an increase of biofilm area and thickness in the early phase of cultivation with a time-delayed biomass production. Hereby, possibly the proportion of EPS and biomass changed in the beginning in combination with biofilm spreading to increase the utilization of available light and aerosol components. Afterwards, biomass production was increased. Concerning pigment composition in the course of cultivation, for T. sociatus all PBPs were degraded, whereas in C. chthonoplastes an increase of all PBPs was measured. This can be explained with the role of PBPs for photosynthesis but as well as nitrogen storage, which is adjusted strain dependent. Summarizing, the shown methods are suitable for phototrophic biofilm characterization in a small scale. This allows the examination of cyanobacteria and a valuation of their suitability for possible production processes.
    Schlagwörter Cyanobacteria ; aerosols ; algae ; area ; biofilm ; biomass production ; carotenoids ; chlorophyll ; correlation ; fluorometry ; industrialization ; light ; microorganisms ; natural dyes ; nitrogen ; photosynthesis ; phycobiliprotein ; polymers ; research ; sampling ; storage ; thickness ; tomography
    Sprache Englisch
    Erscheinungsverlauf 2020-09
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    Anmerkung NAL-light
    ISSN 2211-9264
    DOI 10.1016/j.algal.2020.101996
    Datenquelle NAL Katalog (AGRICOLA)

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  4. Artikel ; Online: Passively immobilized cyanobacteria Nostoc species BB 92.2 in a moving bed photobioreactor (MBPBR): Design, cultivation, and characterization.

    Walther, Jakob / Erdmann, Niklas / Stoffel, Michael / Wastian, Katharina / Schwarz, Anna / Strieth, Dorina / Muffler, Kai / Ulber, Roland

    Biotechnology and bioengineering

    2022  Band 119, Heft 6, Seite(n) 1467–1482

    Abstract: The cyanobacterium Nostoc sp. BB 92.3. had shown antibacterial activity. A cultivation as biofilm, a self-forming matrix of cells and extracellular polymeric substances, increased the antibacterial effect. A new photobioreactor system was developed that ... ...

    Abstract The cyanobacterium Nostoc sp. BB 92.3. had shown antibacterial activity. A cultivation as biofilm, a self-forming matrix of cells and extracellular polymeric substances, increased the antibacterial effect. A new photobioreactor system was developed that allows a surface-associated cultivation of Nostoc sp. as biofilm. High-density polyethylene carriers operated as a moving bed were selected as surface for biomass immobilization. This system, well established in heterotrophic wastewater treatment, was for the first time used for phototrophic biofilms. The aim was a cultivation on a large scale without inhibiting growth while maximizing immobilization. Cultivation in a small photobioreactor (1.5 L) with different volumetric filling degrees of carriers (13.4%-53.8%) in a batch process achieved immobilization rates of 70%-85% and growth was similar to a no-carrier-control. In a larger photobioreactor (65 L) essentially all of the biomass was immobilized on the carriers and the space-time yield of biomass (0.018 g
    Mesh-Begriff(e) Anti-Bacterial Agents ; Biofilms ; Biomass ; Nostoc ; Photobioreactors/microbiology
    Chemische Substanzen Anti-Bacterial Agents
    Sprache Englisch
    Erscheinungsdatum 2022-03-11
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 280318-5
    ISSN 1097-0290 ; 0006-3592
    ISSN (online) 1097-0290
    ISSN 0006-3592
    DOI 10.1002/bit.28072
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 960: Hefte anzeigen Standort:
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  5. Artikel: A Novel Method to Achieve Precision and Reproducibility in Exposure Parameters for Low-Frequency Pulsed Magnetic Fields in Human Cell Cultures.

    Ronniger, Michael / Aguida, Blanche / Stacke, Christina / Chen, Yangmengfan / Ehnert, Sabrina / Erdmann, Niklas / Eschenburg, Georg / Falldorf, Karsten / Pooam, Marootpong / Wing, Anthony / Ahmad, Margaret

    Bioengineering (Basel, Switzerland)

    2022  Band 9, Heft 10

    Abstract: The effects of extremely low-frequency electromagnetic field (ELF-MF) exposure on living systems have been widely studied at the fundamental level and also claimed as beneficial for the treatment of diseases for over 50 years. However, the underlying ... ...

    Abstract The effects of extremely low-frequency electromagnetic field (ELF-MF) exposure on living systems have been widely studied at the fundamental level and also claimed as beneficial for the treatment of diseases for over 50 years. However, the underlying mechanisms and cellular targets of ELF-MF exposure remain poorly understood and the field has been plagued with controversy stemming from an endemic lack of reproducibility of published findings. To address this problem, we here demonstrate a technically simple and reproducible EMF exposure protocol to achieve a standardized experimental approach which can be readily adopted in any lab. As an assay system, we chose a commercially available inflammatory model human cell line; its response to magnetic fields involves changes in gene expression which can be monitored by a simple colorimetric reporter gene assay. The cells were seeded and cultured in microplates and inserted into a custom-built, semi-automated incubation and exposure system which accurately controls the incubation (temperature, humidity, CO2) and magnetic-field exposure conditions. A specific alternating magnetic field (<1.0% spatial variance) including far-field reduction provided defined exposure conditions at the position of each well of the microplate. To avoid artifacts, all environmental and magnetic-field exposure parameters were logged in real time throughout the duration of the experiment. Under these extensively controlled conditions, the effect of the magnetic field on the cell cultures as assayed by the standardized operating procedure was highly reproducible between experiments. As we could fully define the characteristics (frequency, intensity, duration) of the pulsed magnetic field signals at the position of the sample well, we were, for the first time, able to accurately determine the effect of changing single ELF-MF parameters such as signal shape, frequency, intensity and duty cycle on the biological response. One signal in particular (10 Hz, 50% duty cycle, rectangular, bipolar, 39.6μT) provided a significant reduction in cytokine reporter gene expression by 37% in our model cell culture line. In sum, the accuracy, environmental control and data-logging capacity of the semi-automated exposure system should greatly facilitate research into fundamental cellular response mechanisms and achieve the consistency necessary to bring ELF-MF/PEMF research results into the scientific mainstream.
    Sprache Englisch
    Erscheinungsdatum 2022-10-21
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2746191-9
    ISSN 2306-5354
    ISSN 2306-5354
    DOI 10.3390/bioengineering9100595
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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